| Project/Area Number |
16K21133
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Molecular biology
General medical chemistry
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| Research Institution | Kyoto University |
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Principal Investigator |
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| Project Period (FY) |
2016-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2017: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2016: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | 哺乳類 / 始原生殖細胞 / 多能性幹細胞 / エピゲノム / 遺伝子 / ゲノム / クロマチン / エピジェネティクス / 生殖細胞 |
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| Outline of Final Research Achievements |
In mammalian embryos, the specified primordial germ cells undergo epigenetic reprogramming, in which repressive epigenetic marks dynamically change; however, the molecular details remains unknown. Recently, we have developed an in vitro system to induce primordial germ cell-like cells (PGCLCs) using human pluripotent stem cells (PSCs) as starting materials. In this research, we first evaluated various PSC clones regarding the induction efficiency into PGCLCs, and performed comparable analysis of repressive epigenetic states in the selected clones. We further examine the dynamics of repressive chromatin during the in vitro induction process.
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