Development of targeted genome sequencing in microbiome

• Project/Area Number: 16K21144
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: General medical chemistry; Medical genome science
• Research Institution: The University of Tokyo (2017); Osaka University (2016)
• Principal Investigator: Anda Mizue 東京大学, 大学院理学系研究科(理学部), 特任研究員 (60759455)
• Project Period (FY): 2016-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2017: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2016: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
• Keywords: 全ゲノム増幅 / FISH / FACS / ロングリードシークエンス / キメラDNA / ロングリードシークエンサー / phi29 DNAポリメラーゼ / 次世代シークエンサー / 微量DNA / メタゲノム

Outline of Final Research Achievements

The aim of this study was to develop a methodology for targeted genome sequencing in microbiome by using fluorescent activated cell sorting.
This study showed that hybrid assembly of short and long reads improves genome assembly derived from whole genome amplification product.