Analysis of the water splitting and oxigen evolving mechanis of Photosystem II comples using serial femto-second crystallography
| Project/Area Number |
16K21181
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Structural biochemistry
Plant molecular biology/Plant physiology
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| Research Institution | Okayama University |
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Principal Investigator |
Akita Fusamichi 岡山大学, 異分野基礎科学研究所, 准教授 (50751418)
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| Project Period (FY) |
2016-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2017: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2016: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | 光合成 |
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| Outline of Final Research Achievements |
Photosystem II is a supercomplex, and catalyzes water-splitting, leading to the generation of electrons, protons and oxygen. Since no intermediate structures of the S-state has been solved, the mechanism of water-splitting is still not clear. To analyze the structures of the intermediate S-states, we prepared PSII microcrystals and collected their diffraction images using time-resolved serial femtosecond crystallography with X-ray free electron laser at SACLA. We obtained two-flashes illuminated PSII structure at 2.35 angstrom resolution. By comparing with the S1-state structure, it was revealed that a new oxygen atom was inserted into a position close to one of the oxo-bridges (O5) within Mn cluster.
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Report
(3 results)
Research Products
(8 results)
