Novel theranostic strategy against peritoneal metastasis of gastric cancer

• Project/Area Number: 16K21185
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Digestive surgery; Gastroenterology
• Research Institution: Okayama University
• Principal Investigator: Kikuchi Satoru 岡山大学, 大学病院, 助教 (40736584)
• Research Collaborator: ISHIKAWA Wataru
• Project Period (FY): 2016-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2017: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2016: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 胃癌 / 腹膜播種 / 腹腔内投与 / 腫瘍溶解ウイルス / パクリタキセル / 癌 / ウィルス / 外科

Outline of Final Research Achievements

We investigated the efficacy of combination therapy using oncolytic adenovirus; OBP-401 which could replicate only within the tumor cells selectively and express green fluorescent protein (GFP) and chemotherapeutic agent, paclitaxel (PTX). OBP-401 synergistically suppressed the viability of two kinds of human SGC cells in combination with PTX in vitro. And PTX enhanced the efficacy of OBP-401 replication, resulted in synergistic effect. Peritoneal metastatic cells were visualized as GFP-positive cells selectively by the intra-peritoneal (i.p.) administration of OBP-401 in the orthotopic mouse model. The combination therapy with i.p. administration of OBP-401 and PTX could also significantly inhibited the tumor growth in vivo.

Research Products

• [Journal Article] A Case of Multiple Liver Metastasis from Gastric Cancer Successfully Treated with CPT-11 as Third-Line Chemotherapy (2016)
  • Author(s): Kikuchi S, Nishizaki M, Kuroda S, Kagawa S, Fujiwara T
  • Journal Title: Gan To Kagaku Ryoho Volume: 43 Pages: 2219-2221
  • Peer Reviewed
• [Journal Article] Recurrence after Endoscopic Curative Resection of Mucosal Gastric Cancer Associated with an Adjacent Neoplastic Precursor Lesion. (2016)
  • Author(s): Kikuchi S, Kagawa S, Ohara T, Kubota T, Kuwada K, Kagawa T, Kuroda S, Shirakawa Y, Nishizaki M, Fujiwara T.
  • Journal Title: Acta Med Okayama Volume: 70 Pages: 213-216
  • NAID: 120005770690
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] A case of right-sided Bochdalek hernia incidentally diagnosed in a gastric cancer patient. (2016)
  • Author(s): Kikuchi S, Nishizaki M, Kuroda S, Kagawa S, Fujiwara T
  • Journal Title: BMC Surg Volume: 16 Issue: 1 Pages: 34-34
  • DOI: 10.1186/s12893-016-0145-2
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Targeted photodynamic virotherapy armed with a genetically encoded photosensitizer. (2016)
  • Author(s): Takehara K, Kagawa S, Fujiwara T, et al.
  • Journal Title: Molecular Cancer Therapeutics Volume: 15 Issue: 1 Pages: 199-208
  • DOI: 10.1158/1535-7163.mct-15-0344
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Presentation] Novel theranostic strategy against scirrhous gastric cancer: combination with fluorescence oncolytic adenovirus and chemotherapy (2018)
  • Author(s): Wataru Ishikawa, Satoru Kikuchi, Hiroshi Tazawa, Toshaki Ohara, Shinji Kuroda, Kazuhiro Noma, Hiroyuki Kishimoto, Masahiko Nishizaki, Yasuo Urata, Shunsuke Kagawa, Toshiyoshi Fujiwara
  • Organizer: AACR Annual Meeting 2018
  • Int'l Joint Research
• [Presentation] スキルス胃癌の腹膜播種に対する新たな診断的治療戦略：腫瘍融解型アデノウイルスと化学療法の融合 (2018)
  • Author(s): 石川 亘、菊地 覚次、田澤 大、大原 利章、黒田 新士、野間 和広、西崎 正彦、香川 俊輔、藤原 俊義
  • Organizer: 第73回日本消化器外科学会
• [Presentation] Novel theranostic strategy against scirrhous gastric cancer; combination of chemotherapy and fluorescence oncolytic adenovirus (2017)
  • Author(s): Wataru Ishikawa, Satoru Kikuchi, Hiroshi Tazawa, Shinji Kuroda, Kazuhiro Noma, Hiroyuki Kishimoto, Takeshi Nagasaka, Masahiko Nishizaki, Shunsuke Kagawa, Toshiyoshi Fujiwara
  • Organizer: AACR Annual Meeting 2017
  • Int'l Joint Research
• [Presentation] Novel theranostic strategy with combination of fluorescence oncolytic adenovirus and chemotherapy for peritoneal metastasis of gastric cancer (2017)
  • Author(s): Wataru Ishikawa, Satoru Kikuchi, Hiroshi Tazawa, Shinji Kuroda, Kazuhiro Noma, Hiroyuki Kishimoto, Takeshi Nagasaka, Masahiko Nishizaki, Yasuo Urata, Shunsuke Kagawa, Toshiyoshi Fujiwara
  • Organizer: 第23回日本遺伝子細胞治療学会
• [Presentation] Novel theranostic strategy : combination of fluorescence oncolytic virus and chemotherapy for scirrhous gastric cancer (2017)
  • Author(s): Wataru Ishikawa, Satoru Kikuchi, Hiroshi Tazawa, Shinji Kuroda, Kazuhiro Noma, Hiroyuki Kishimoto, Takeshi Nagasaka, Masahiko Nishizaki, Yasuo Urata, Shunsuke Kagawa, Toshiyoshi Fujiwara
  • Organizer: 第76回日本癌学会
• [Presentation] リンパ節転移分布からみた残胃進行癌の治療戦略 (2016)
  • Author(s): 菊地覚次 黒田新士 渡邉めぐみ 白川靖博 西﨑正彦 香川俊輔 藤原俊義
  • Organizer: 第71回日本消化器外科学会総会
  • Place of Presentation: 徳島
  • Year and Date: 2016-07-14