Search for early marker of Osteoarthritis using Exosome and establish monitoring index of exercise and nutritional effects
| Project/Area Number |
16K21192
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Applied health science
Laboratory animal science
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| Research Institution | Kyoto Sangyo University (2017)
The University of Tokyo (2016) |
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Principal Investigator |
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| Research Collaborator |
MIYAKI Shigeru 広島大学, 病院, 講師 (10392490)
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| Project Period (FY) |
2016-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2017: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | 変形性関節症 / microRNA / カルノシン酸 / Exosome / 運動 / 老化 / 食品 |
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| Outline of Final Research Achievements |
In this study, we explored the effect of carnosic acid (CA) on osteoarthritis (OA) using human synovial fibroblast and human chondrocytes. The expression of heme oxygenase-1(HO-1) was upregulated in human synovial fibroblasts and chondrocytes treated with CA in a dose-dependent manner. CA had no effect on the expression of Col2a1 in chondrocytes. CA suppressed the expression of OA-related genes in human synovial fibroblast and chondrocytes.In addition, the expression of the cartilage-degrading enzyme was suppressed. We found that CA activates miR-140 which maintains cartilage homeostasis and activates HO-1 by suppressing the expression of Bach1 gene as its molecular mechanism. These findings suggest that CA is an effective HO-1 inducer and have a potential to be a supplement for OA prevention.
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Report
(3 results)
Research Products
(8 results)
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[Journal Article] A novel Mucidosphaerium sp. from Beppu hot spring down-regulates inflammatory gene expression in skin and articular cells2018
Author(s)
Miyata M, Iwata S, Mifude C, Tajima M, Kameyama M, Ihara M, Matsui T, Yamagishi S, Ishitobi H, Miyaki S, Kaseda K
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Journal Title
Alternative therapies in health and medicine
Volume: in press
Related Report
Peer Reviewed / Open Access
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