| Project/Area Number |
16K21192
|
|---|
| Research Category |
Grant-in-Aid for Young Scientists (B)
|
| Allocation Type | Multi-year Fund |
|---|
| Research Field |
Applied health science
Laboratory animal science
|
|---|
| Research Institution | Kyoto Sangyo University (2017)
The University of Tokyo (2016) |
|---|
Principal Investigator |
|
|---|
| Research Collaborator |
MIYAKI Shigeru 広島大学, 病院, 講師 (10392490)
|
|---|
| Project Period (FY) |
2016-04-01 – 2018-03-31
|
| Project Status |
Completed (Fiscal Year 2017)
|
| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2017: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
|
|---|
| Keywords | 変形性関節症 / microRNA / カルノシン酸 / Exosome / 運動 / 老化 / 食品 |
|---|
| Outline of Final Research Achievements |
In this study, we explored the effect of carnosic acid (CA) on osteoarthritis (OA) using human synovial fibroblast and human chondrocytes. The expression of heme oxygenase-1(HO-1) was upregulated in human synovial fibroblasts and chondrocytes treated with CA in a dose-dependent manner. CA had no effect on the expression of Col2a1 in chondrocytes. CA suppressed the expression of OA-related genes in human synovial fibroblast and chondrocytes.In addition, the expression of the cartilage-degrading enzyme was suppressed. We found that CA activates miR-140 which maintains cartilage homeostasis and activates HO-1 by suppressing the expression of Bach1 gene as its molecular mechanism. These findings suggest that CA is an effective HO-1 inducer and have a potential to be a supplement for OA prevention.
|
