Research Project
Grant-in-Aid for Young Scientists (B)
Using genome editing technology, knock-in vector containing eGFP gene under cricket minimal promoter was introduced into Hox gene loci in the cricket genome. eGFP fluorescent was observed as expected pattern. We succeeded to establish the method for generating enhancer trap line in the cricket. For introducing correctly knock-in direction and junction, knock-in method via MMEJ pathway was applied in the cricket. eGFP gene was correctly introduced into stop codon loci in target gene via MMEJ mediated knock-in, and eGFP expression was observed. In addition, RNA-seq analysis was performed using PacBioRSII. Extracted mRNA from cricket embryo and nymph was fractionated into four, and each fraction was sequenced in 4 cells. As a result, 760,000 reads and 2.25 Gb sequence was obtained in total.
All 2017 2016
All Journal Article (5 results) (of which Int'l Joint Research: 1 results, Peer Reviewed: 4 results, Open Access: 4 results, Acknowledgement Compliant: 2 results) Presentation (3 results) (of which Int'l Joint Research: 2 results) Book (1 results) Patent(Industrial Property Rights) (1 results)
Scientific Reports
Volume: 7 Issue: 1 Pages: 7330-7336
10.1038/s41598-017-07520-1
Volume: 7 Issue: 1 Pages: 507-507
10.1038/s41598-017-00501-4
Methods in Molecular Biology
Volume: 1630 Pages: 219-233
10.1007/978-1-4939-7128-2_18
Volume: 6 Issue: 1 Pages: 26685-26685
10.1038/srep26685
120006240041
Proc Nati Acad Sci USA
Volume: 113 Issue: 20 Pages: 5634-5639
10.1073/pnas.1600612113
120007168252
