Comparative proteomics of FFPE tissue for Exploring biomarker of well differentiated liposarcomas

• Project/Area Number: 16K21250
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Human pathology; Tumor diagnostics
• Research Institution: Sapporo Medical University
• Principal Investigator: Aoyama Tomoyuki 札幌医科大学, 医学部, 研究員 (50737781)
• Project Period (FY): 2016-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000); Fiscal Year 2017: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: FFPE / ALT/WDL / Proteomics / ALT/WDLS / プロテオミクス / IHC / プロてオミクス解析 / 病理診断 / プロテオミクス解析 / 診断病理学

Outline of Final Research Achievements

In this study, comparable proteome analysis of FFPE tissues revealed multiple candidate marker molecules for differentiating atypical lipomatous tumor/well-differentiated liposarcoma (ALT/WDL) from lipoma. We identified 181 unique proteins for ALT/WDL. Of the identified proteins, we finally focused on CCDC180 and LRRC4 as candidate markers of ALT/WDL. We showed that CCDC180 and LRRC4 immunohistochemistry clearly stained tumor cells of ALT/WDL and dedifferentiated liposarcoma and could differentiate them from lipoma with high accuracy. We also used cell biological methods to further examine the expression of the CCDC180 and LRRC4 in liposarcoma cells.
Their expression was associated with adipocyte differentiation and contributed to malignant potentials of liposarcoma cells.

Research Products

• [Journal Article] Prognostic significance of the co-expression of EGFR and HER2 in adenocarcinoma of the uterine cervix. (2017)
  • Author(s): Ueda A, Takasawa A, Akimoto T, Takasawa K, Aoyama T, Ino Y, Nojima M, Ono Y, Murata M, Osanai M, Hasegawa T, Saito T, Sawada N.
  • Journal Title: PLoS One Volume: 12(8) Issue: 2 Pages: 172-180
  • DOI: 10.1016/j.yexcr.2018.03.018
  • Peer Reviewed
• [Journal Article] Epigenetic silencing of SMOC1 in traditional serrated adenoma and colorectal cancer. (2017)
  • Author(s): Aoki H, Yamamoto E, Takasawa A, Niinuma T, Yamano HO, Harada T, Matsushita HO, Yoshikawa K, Takagi R, Harada E, Tanaka Y, Yoshida Y, Aoyama T, Eizuka M, Yorozu A, Kitajima H, Kai M, Sawada N, Sugai T, Nakase H, Suzuki H.
  • Journal Title: Oncotarget. Volume: 9 Issue: 4 Pages: 4707-4721
  • DOI: 10.18632/oncotarget.23523
  • Peer Reviewed / Open Access
• [Journal Article] Claudin-18 coupled with EGFR/ERK signaling contributes to the malignant potentials of bile duct cancer. (2017)
  • Author(s): Takasawa K, Takasawa A, Osanai M, Aoyama T, Ono Y, Kono T, Hirohashi Y, Murata M, Sawada N.
  • Journal Title: Cancer Lett. Volume: 403 Pages: 66-73
  • DOI: 10.1016/j.canlet.2017.05.033
  • Peer Reviewed
• [Journal Article] Diagnostic utility of automated SureFISH (Dako Omnis) in the diagnosis of musculoskeletal translocation-related sarcomas. (2017)
  • Author(s): 22.Sugita S, Aoyama T, Ito Y, Asanuma H, Sugawara T, Segawa K, Ito Y, Kikuchi N, Tsujiwaki M, Fujita H, Ono Y, Hasegawa T
  • Journal Title: Pathol Int Volume: 67 Issue: 10 Pages: 510-513
  • DOI: 10.1111/pin.12562
  • Peer Reviewed
• [Journal Article] Prognostic significance of the co-expression of EGFR and HER2 in adenocarcinoma of the uterine cervix. (2017)
  • Author(s): 23.Ueda A, Takasawa A, Akimoto T, Takasawa K, Aoyama T, Ino Y, Nojima M, Ono Y, Murata M, Osanai M, Hasegawa T, Saito T, Sawada N
  • Journal Title: PLoS One Volume: 12 Pages: 1-13
  • Peer Reviewed / Open Access
• [Journal Article] NUTM2A-CIC fusion small round cell sarcoma: A genetically distinct variant of CIC-rearranged sarcoma (2017)
  • Author(s): Sugita S, Arai Y, Aoyama T, Asanuma H, Mukai W, Hama N, Emori M, Shibata T, Hasegawa T
  • Journal Title: Human Pathology Volume: 印刷中 Pages: 225-230
  • DOI: 10.1016/j.humpath.2017.01.012
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Diagnostic utility of FOSB immunohistochemistry in pseudomyogenic hemangioendothelioma and its histological mimics. (2016)
  • Author(s): Shintaro Sugita, Hiroshi Hirano, Noriaki Kikuchi, Terufumi Kubo, Hiroko Asanuma, Tomoyuki Aoyama, Makoto Emori, Tadashi Hasegawa
  • Journal Title: Diagnostic Pathology Volume: 75 Issue: 1
  • DOI: 10.1186/s13000-016-0530-2
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Analysis of the expression and localization of tight junction transmembrane proteins, claudin-1, -4, -7, occludin and JAM-A, in human cervical adenocarcinoma (2016)
  • Author(s): Taishi Akimoto, Akira Takasawa, Masaki Murata, Yui Kojima, Kumi Takasawa, Masanori Nojima, Tomoyuki Aoyama, Yutaro Hiratsuka, Yusuke Ono, Satoshi Tanaka, Makoto Osanai, Tadashi Hasegawa, Tsuyoshi Saito, Norimasa Sawada
  • Journal Title: HISTOLOGY AND HISTOPATHOLOGY Volume: 31 Pages: 921-931
  • Peer Reviewed
• [Presentation] 平滑筋肉腫のFFPE組織標本を用いた新規バイオマーカー探索の試み (2017)
  • Author(s): 青山智志、高澤 啓、伊野善彦、小野佑輔、村田雅樹、小山内 誠、長谷川 匡、 澤田典均
  • Organizer: 第42回日本医用マススペクトル学会年会
• [Presentation] 平滑筋肉腫のFFPE組織標本を用いた 新規バイオマーカー探索の試み (2016)
  • Author(s): 青山 智志、高澤 啓、村田 雅樹、中西 敬太郎、 小山内 誠、長谷川 匡、澤田 典均
  • Organizer: 第96回 北海道医学大会病理部会・第49回 北海道病理談話会
  • Place of Presentation: 北大医学部学友会館(北海道札幌市)
• [Presentation] FFPE組織を用いた比較プロテオミクスによる高分化型脂肪肉腫のバイオマーカー探索 (2016)
  • Author(s): 青山 智志、高澤 啓、村田 雅樹、中西 敬太郎、田中 敏、小山内 誠、長谷川 匡、澤田 典
  • Organizer: 第105回 日本病理学会総会
  • Place of Presentation: 仙台国際センター(宮城県仙台市)