| Project/Area Number |
16K21419
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Immunology
Biological pharmacy
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| Research Institution | Hoshi University |
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Principal Investigator |
Oku Teruaki 星薬科大学, 薬学部, 講師 (20409361)
|
|---|
| Research Collaborator |
ANDO Yusuke
KANEKO Yutaka
NAMIKI Toshikazu
HIRAI Satoshi
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| Project Period (FY) |
2016-04-01 – 2018-03-31
|
| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2016: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | Coronin-1 / 結核 / リン酸化 / 細胞内寄生 / LpdC / 結核菌 / アクチン結合タンパク質 / PKC / 貪食 / 免疫学 / 感染症 / 細胞内寄生菌 |
|---|
| Outline of Final Research Achievements |
The interaction between Coronin-1 in host cells and Lipoamide dehydrogenase C (LpdC) from mycobacteria is considered the cause of intracellular parasitism of pathogenic mycobacteria. In this study, we demonstrated the direct association of Coronin-1 and LpdC which are recombinant proteins by pull-down assay. And this association was indicated possible regulation by phosphorylation of Thr-412 on Coronin-1. Furthermore, we elucidated that LpdA which is M. smegmatis (non-pathogenic mycobacteria) homologue of LpdC also associated with Coronin-1. This result suggested that differences other than association with Coronin-1 of Lpds are involved in intracellular survival.
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