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Structural and functional analysis of hexametric ATPases.

Research Project

Project/Area Number 16K21472
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Functional biochemistry
Biophysics
Research InstitutionKyoto Sangyo University

Principal Investigator

KISHIKAWA Jun-ichi  京都産業大学, 総合生命科学部, 研究助教 (80599241)

Research Collaborator MITSUOKA Kaoru  
UCHIHASHI Takayuki  
Project Period (FY) 2016-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2016: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Keywords6 量体 ATPase / V-ATPase / 回転分子モーター / 単粒子解析 / 1分子観察 / 電子顕微鏡 / 低温電子顕微鏡 / 6量体 ATPase / AAA+-ATPase / 6量体ATPase / 高速原子間力顕微鏡
Outline of Final Research Achievements

Hexametric ATPases, like AAA+-ATPase, play important roles in cell. The conformational changes of hexametric ATPases associated with ATP hydrolysis is essential for its function. However, the mechanisms of the conformational changes are remains unknown. In this study, I attempted to reveal the mechanism by single-molecule-analysis (SPA) using cryogenic electron microscopy and single molecule direct observation technique.
I solved three structures of V-ATPase, a kind of hexametric ATPase, by SPA. From this result, the catalytic mechanism of V-ATPase was partially revealed. In addition, to investigate the interaction between hexametric ATPase and its substrate, I designed artificial substrate and measured the interaction. As the result, it is strongly indicated that the interaction among them is rough rather than specific and robust.

Report

(3 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Research-status Report
  • Research Products

    (17 results)

All 2018 2017 2016 Other

All Journal Article (4 results) (of which Peer Reviewed: 3 results,  Open Access: 2 results) Presentation (11 results) (of which Invited: 3 results) Remarks (2 results)

  • [Journal Article] Cryo EM structure of intact rotary H+-ATPase/synthase from Thermus thermophilus.2018

    • Author(s)
      #Nakanishi A, #Kishikawa J (#共同筆頭著者), Tamakoshi M, Mitsuoka K, Yokoyama K.
    • Journal Title

      Nat Commun.

      Volume: 9 Issue: 1 Pages: 89-89

    • DOI

      10.1038/s41467-017-02553-6

    • NAID

      40021660371

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] General anesthetics cause mitochondrial dysfunction and reduction of intracellular ATP levels.2018

    • Author(s)
      Kishikawa JI, Inoue Y, Fujikawa M, Nishimura K, Nakanishi A, Tanabe T, Imamura H, Yokoyama K.
    • Journal Title

      PLoS ONE

      Volume: 13 Issue: 1 Pages: e0190213-e0190213

    • DOI

      10.1371/journal.pone.0190213

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] 低温電子顕微鏡を用いた回転分子モーター V 型 ATPase の単粒子解析.2018

    • Author(s)
      岸川 淳一
    • Journal Title

      顕微鏡

      Volume: 印刷中

    • NAID

      130007686938

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] クライオ電子顕微鏡による液胞型 ATPase (VoV1) の構造解析2017

    • Author(s)
      岸川淳一、中西温子、横山謙
    • Journal Title

      京都産業大学 総合学術研究所所報

      Volume: 印刷中

    • Related Report
      2016 Research-status Report
  • [Presentation] Single particle analysis of V-type ATP synthase2017

    • Author(s)
      Jun-ichi Kishikawa, Atsuko Nakanishi, Kaoru Mitsuoka, Ken Yokoyama
    • Organizer
      The 1st ABiS Symposium
    • Place of Presentation
      自然科学研究機構 岡崎コンファレンスセンター(愛知県・岡崎市)
    • Year and Date
      2017-02-19
    • Related Report
      2016 Research-status Report
  • [Presentation] 低温電子顕微鏡を用いたトモグラフィ法によるミトコンドリア膜タンパク質複合体の構造解析の試み2017

    • Author(s)
      岸川 淳一、中西 温子、光岡 薫、横山 謙
    • Organizer
      第43回生体エネルギー研究会討論会
    • Related Report
      2017 Annual Research Report
  • [Presentation] クライオ EM による好熱菌由来 V 型 ATP 合成酵素の単粒子解析2017

    • Author(s)
      岸川 淳一、中西 温子、光岡 薫、横山 謙
    • Organizer
      ConBio2017
    • Related Report
      2017 Annual Research Report
  • [Presentation] de novo 設計した人工コイルドコイルは回転子として機能する2017

    • Author(s)
      岸川 淳一、 馬場 みほ里、 中西 温子、 横山 謙
    • Organizer
      第55回日本生物物理学会年会
    • Related Report
      2017 Annual Research Report
  • [Presentation] Artificial design of rotary axis reveals the rotation mechanism of rotary motor.2017

    • Author(s)
      岸川 淳一、馬場 みほ里、中西 温子、横山 謙
    • Organizer
      第17回日本蛋白質科学会年会
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] Single Particle Analysis of V-type ATP Synthase.2017

    • Author(s)
      Jun-cihi Kishikawa
    • Organizer
      he 1st ABiS Symposium -Towards the Future of Advanced Bioimaging for Life Sciences-
    • Related Report
      2017 Annual Research Report
  • [Presentation] 人工回転軸の設計とその回転2016

    • Author(s)
      岸川淳一
    • Organizer
      第42回日本生体エネルギー研究会
    • Place of Presentation
      名古屋工業大学(愛知県・名古屋市)
    • Year and Date
      2016-12-19
    • Related Report
      2016 Research-status Report
  • [Presentation] 全身麻酔薬は細胞内ATPレベルを減少させる2016

    • Author(s)
      岸川淳一、井上勇奎、藤川誠、中西温子、今村博臣、横山謙
    • Organizer
      第39回日本分子生物学会年会
    • Place of Presentation
      パシフィコ横浜(神奈川県・横浜市)
    • Year and Date
      2016-11-30
    • Related Report
      2016 Research-status Report
  • [Presentation] De novo 設計軸の回転から明らかになったトルク発生機構2016

    • Author(s)
      岸川淳一、馬場みほ里、中西温子、横山謙
    • Organizer
      第54回 日本生物物理学会年会
    • Place of Presentation
      つくば国際会議場(茨城県・つくば市)
    • Year and Date
      2016-11-25
    • Related Report
      2016 Research-status Report
    • Invited
  • [Presentation] The relationship between general anesthetic effects and ATP level in cells.2016

    • Author(s)
      Jun-ichi Kishikawa, Yuki Inoue, Atsuko Nakanishi, Makoto Fujikawa, Hiromi Imamura, Ken Yokoyama
    • Organizer
      European Bioenergetics Conference 2016
    • Place of Presentation
      イタリア・リーヴァ・デル・ガルダ
    • Year and Date
      2016-07-02
    • Related Report
      2016 Research-status Report
  • [Presentation] De novo 設計人工軸の回転とそこから明らかになったトルク発生の仕組み2016

    • Author(s)
      岸川淳一、馬場みほ里、中西温子、横山謙
    • Organizer
      第16回 日本蛋白質科学会年会
    • Place of Presentation
      福岡国際会議場(福岡県・福岡市)
    • Year and Date
      2016-06-07
    • Related Report
      2016 Research-status Report
    • Invited
  • [Remarks] 京都産業大学 生体膜エネルギー研究室

    • URL

      http://www.cc.kyoto-su.ac.jp/~yokoken/index-j.htm

    • Related Report
      2017 Annual Research Report
  • [Remarks] 京都産業大学 研究者データベース

    • URL

      https://gyoseki.kyoto-su.ac.jp/ktsuhp/KgApp?kyoinId=ymbygdoeggy

    • Related Report
      2017 Annual Research Report

URL: 

Published: 2016-04-21   Modified: 2019-03-29  

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