Structural study of FIP200, a scaffold protein of mammalian autophagy initiation complex

• Project/Area Number: 16K21593
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Structural biochemistry; Physical pharmacy
• Research Institution: Microbial Chemistry Research Foundation
• Principal Investigator: SUZUKI Hironori 公益財団法人微生物化学研究会, 微生物化学研究所, 博士研究員 (20625694)
• Project Period (FY): 2016-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2017: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2016: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: X線結晶構造解析 / オートファジー / Ｘ線結晶構造解析

Outline of Final Research Achievements

A lot of proteins involved in autophagy are widely conserved in eukaryotes from budding yeast to humans. However, some proteins are unique factors to higher eukaryotes such as human. One of which is FIP200, a scaffold protein of mammalian autophagy initiation complex.
In this study, we first prepared to stable region to use biochemical analysis and crystallographic studies. Moreover, we crystallized and determined the structure of a part of FIP200. The C-terminal region of FIP200 formed a parallel coiled-coil dimer. This result indicates that the large protein FIP200 functioning as a scaffold protein assumes a parallel dimeric structure.

Research Products

• [Journal Article] Atg7 Activates an Autophagy-Essential Ubiquitin-like Protein Atg8 through Multi-Step Recognition (2018)
  • Author(s): Yamaguchi Masaya、Satoo Kenji、Suzuki Hironori、Fujioka Yuko、Ohsumi Yoshinori、Inagaki Fuyuhiko、Noda Nobuo N.
  • Journal Title: Journal of Molecular Biology Volume: 430 Issue: 3 Pages: 249-257
  • DOI: 10.1016/j.jmb.2017.12.002
  • Peer Reviewed
• [Journal Article] Biophysical characterization of Atg11, a scaffold protein essential for selective autophagy in yeast (2017)
  • Author(s): Suzuki, H. and *Noda, N. N.
  • Journal Title: FEBS Open Bio Volume: 8 Issue: 1 Pages: 110-116
  • DOI: 10.1002/2211-5463.12355
  • Peer Reviewed / Open Access
• [Journal Article] Structural biology of the core autophagy machinery. (2017)
  • Author(s): Suzuki H, Osawa T, Fujioka Y, Noda NN
  • Journal Title: Current Opinion in Structural Biology Volume: 43 Pages: 10-17
  • DOI: 10.1016/j.sbi.2016.09.010
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] The Intrinsically Disordered Protein Atg13 Mediates Supramolecular Assembly of Autophagy Initiation Complexes. (2016)
  • Author(s): Yamamoto H, Fujioka Y, Suzuki SW, Noshiro D, Suzuki H, Kondo-Kakuta C, Kimura Y, Hirano H, Ando T, Noda NN, Ohsumi Y
  • Journal Title: Developmental Cell Volume: 38 Issue: 1 Pages: 86-99
  • DOI: 10.1016/j.devcel.2016.06.015
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Presentation] Structure of Atg101 in complex with Atg13: Functional insights into mammalian autophagy initiation (2016)
  • Author(s): Suzuki H, Kaizuka T, Mizushima N, Noda NN
  • Organizer: 第42回内藤コンファレンス 生命科学に革命をもたらす最先端構造生物学
  • Place of Presentation: シャトレーゼガトーキングダムサッポロ(北海道札幌市)
  • Year and Date: 2016-10-04
  • Int'l Joint Research