Development of holographic X-ray diffraction imaging method and application to structural study of cell division in an unicellular algae

• Project/Area Number: 16K21621
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Biological physics/Chemical physics/Soft matter physics; Quantum beam science
• Research Institution: University of Hyogo
• Principal Investigator: Takayama Yuki 兵庫県立大学, 物質理学研究科, 助教 (40710132)
• Project Period (FY): 2016-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2017: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2016: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
• Keywords: コヒーレントX線回折イメージング / X線自由電子レーザー / ホログラフィー / 細胞分裂 / 凍結水和試料 / 凍結水和 / 生体試料 / 細胞・オルガネラ

Outline of Final Research Achievements

I developed a holographic diffraction imaging method with an X-ray laser and applied it to structural analysis of primitive red algae cells for understanding of structural basis of cell division, especially focusing on organelle interplay. Imaging red algae cells in interphase and M phase at spatial resolutions of 110 - 200 nm revealed common structural features of organelles and also visualized changes in the electron density distribution in a nucleus associating with cell division without sectioning and/or staining.

Research Products

• [Journal Article] Atmospheric coherent X-ray diffraction imaging for in-situ structural analysis at SPring-8 Hyogo beamline BL24XU (2018)
  • Author(s): Yuki Takayama, Yuki Takami, Keizo Fukuda, Takamasa Miyagawa and Yasushi Kagoshima
  • Journal Title: Journal of Synchrotron Radiation Volume: 印刷中
  • Peer Reviewed
• [Journal Article] Common structural features of toxic intermediates from α-synuclein and GroES fibrillogenesis detected using cryogenic coherent X-ray diffraction imaging (2017)
  • Author(s): H. Kameda, S. Usugi, M. Kobayashi, N. Fukui, S. Lee, K. Hongo, T. Mizobata, Y. Sekiguchi, Y. Masaki, A. Kobayashi, T. Oroguchi, M. Nakasako, Y. Takayama, M. Yamamoto, and Y. Kawata
  • Journal Title: Journal of Biochemistry (Tokyo) Volume: １６１ Issue: 1 Pages: 55-65
  • DOI: 10.1093/jb/mvw052
  • NAID: 40021091513
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] TAKASAGO-6 apparatus for cryogenic coherent X-ray diffraction imaging of biological non-crystalline particles using X-ray free electron laser at SACLA (2016)
  • Author(s): A. Kobayashi, Y. Sekiguchi, Y. Takayama, T. Oroguchi, K. Shirahama, Y. Torizuka, M. Manoda, M. Nakasako* and M. Yamamoto
  • Journal Title: Review of Scientific Instruments Volume: 87 Issue: 5 Pages: 053109-053109
  • DOI: 10.1063/1.4948317
  • Peer Reviewed / Acknowledgement Compliant
• [Presentation] Coherent X-ray Diffraction Imaging at SPring-8 Hyogo Beamline BL24XU (2017)
  • Author(s): Yuki Takayama, Yuki Takami, Keizo Fukuda, Takamasa Miyagawa, Yoshimasa Urushihara, Shigeo Kuwamoto and Yasushi Kagoshima
  • Organizer: The 24th Congress of the International Commission for Optics
  • Int'l Joint Research
• [Presentation] Coherent X-ray Diffraction Imaging at SPring-8 Hyogo Beamline BL24XU (2017)
  • Author(s): Yuki Takayama, Yuki Takami, Takamasa Miyagawa and Yasushi Kagoshima
  • Organizer: International Conference on X-ray Optics and Application 2017
  • Int'l Joint Research
• [Presentation] XFELによる細胞の構造解析に向けたホログラフィックX線回折イメージング法の開発 (2017)
  • Author(s): 高山裕貴、山本雅貴、米倉功治、小林周、関口優希、岡島公司、苙口友隆、中迫雅由、乾弥生、松永幸大
  • Organizer: 第30回日本放射光学会年会・放射光科学合同シンポジウム
  • Place of Presentation: 神戸芸術センター、兵庫県神戸市
• [Presentation] Cryogenic coherent diffraction imaging of biological samples with synchrotron and X-ray laser (2016)
  • Author(s): Yuki Takayama, Masaki Yamamoto, Koji Yonekura, Yuki Sekiguchi, Amane Kobayashi, Koji Okajima, Tomotaka Oroguchi, Masayoshi Nakasako, Yayoi Inui and Sachihiro Matsunaga
  • Organizer: 13th International Conference on X-ray Microscopy
  • Place of Presentation: University of Oxford, Oxford, UK
  • Int'l Joint Research
• [Presentation] X線レーザーと放射光による細胞内空間階層構造のイメージング研究 (2016)
  • Author(s): 高山裕貴
  • Organizer: 多重極限物質科学研究センター・高度産業科学技術研究所合同研究会
  • Place of Presentation: 兵庫県立先端科学技術支援センター、兵庫県赤穂郡上郡町