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Reconstructing epithelial invagination by a wave of ERK activity

Research Project

Project/Area Number 16K21629
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Developmental biology
Cell biology
Research InstitutionInstitute of Physical and Chemical Research

Principal Investigator

Ogura Yosuke  国立研究開発法人理化学研究所, 生命機能科学研究センター, 基礎科学特別研究員 (20733158)

Project Period (FY) 2016-04-01 – 2019-03-31
Project Status Completed (Fiscal Year 2018)
Budget Amount *help
¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Fiscal Year 2018: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2017: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Keywords器官形成 / 細胞間シグナル伝達 / 形態形成 / 上皮 / Sos / Ras / ERK / 画像解析 / 上皮の陥入 / MAPK / 数理モデル / 細胞骨格 / アクトミオシン / 光遺伝学 / 細胞間情報伝達
Outline of Final Research Achievements

Extracellular signal-regulated kinase (ERK) regulates a wide variety of phenomenon including cell proliferation, cell movement and cell differentiation. The complex changes in the spatiotemporal patterns of ERK activity occurring in vivo have yet to be elucidated with respect to its role in the regulation of developmental phenomenon. This study clarified how the propagation of ERK activity during epithelial invagination regulates this morphogenetic movement. Specifically, we obtained the following achievements: (1) The positive feedback in the ERK signaling pathway that enables the propagation of ERK activity and its contribution to the regulation of invagination was clarified. (2) The method of FRET image analysis was established and applied to clarify the difference of ERK activity in the cytoplasmic and nuclear compartments.

Academic Significance and Societal Importance of the Research Achievements

再生医学においては、適切な形と大きさをもつ器官を試験管内で再構築する技術基盤の確立が期待されている。今回得られた研究成果は、器官の自律的発生の背景にある分子・細胞レベルの仕組みの一端を解明した。このような知見は、モデル生物の発生の理解にとどまらず、上記のような医療の基盤技術の確立にも応用が期待される。このような、学術的・社会的意義の側面については、理化学研究所のウェブサイトのプレスリリースとして掲載し、神戸新聞でも報道された。

Report

(4 results)
  • 2018 Annual Research Report   Final Research Report ( PDF )
  • 2017 Research-status Report
  • 2016 Research-status Report
  • Research Products

    (11 results)

All 2019 2018 2017 2016

All Journal Article (3 results) (of which Peer Reviewed: 3 results,  Open Access: 1 results,  Acknowledgement Compliant: 1 results) Presentation (8 results) (of which Int'l Joint Research: 1 results,  Invited: 1 results)

  • [Journal Article] Automated FRET quantification shows distinct subcellular ERK activation kinetics in response to graded EGFR signaling in Drosophila2019

    • Author(s)
      Yosuke Ogura, Mustafa M. Sami, Housei Wada , Shigeo Hayashi
    • Journal Title

      Genes to Cells

      Volume: 24 Issue: 4 Pages: 297-306

    • DOI

      10.1111/gtc.12679

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A switch-like activation relay of EGFR-ERK signaling regulates a wave of cellular contractility for epithelial invagination.2018

    • Author(s)
      Yosuke Ogura, Fu-Lai Wen, Mustafa M. Sami, Tatsuo Shibata, Shigeo Hayashi
    • Journal Title

      Developmental Cell

      Volume: 46 Issue: 2 Pages: 162-172

    • DOI

      10.1016/j.devcel.2018.06.004

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] IKKε inhibits PKC to promote Fascin-dependent actin 2016bundling2016

    • Author(s)
      Otani T, Ogura Y, Misaki K, Maeda T, Kimpara A, Yonemura S, Hayashi S
    • Journal Title

      Development

      Volume: 143 Issue: 20 Pages: 3806-3816

    • DOI

      10.1242/dev.138495

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Presentation] Iterative relay of epidermal growth factor receptor signaling regulates epithelial invagination via a wave of cellular contractility2018

    • Author(s)
      Yosuke Ogura, Fu-Lai Wen, Mustafa M. Sami, Tatsuo Shibata, Shigeo Hayashi
    • Organizer
      第70回日本発生生物学会(日本細胞生物学会合同大会)
    • Related Report
      2018 Annual Research Report
  • [Presentation] ショウジョウバエ胚発生過程におけるEGFR-ERKシグナル活性化のダイナミクス2018

    • Author(s)
      小椋 陽介
    • Organizer
      細胞集団運動の統合的理解に向けたメカノバイオロジー研究の国際共同ネットワーク構築(SPIRITS: 「知の越境」融合チーム研究プログラム)
    • Related Report
      2018 Annual Research Report
    • Invited
  • [Presentation] Intercellular propagation of EGF-ERK signaling by relay mechanism directs epithelial invagination2017

    • Author(s)
      Yosuke Ogura, Fu-Lai Wen, Mustafa M. Sami, Tatsuo Shibata, Shigeo Hayashi
    • Organizer
      Taiwan-Japan Joint Meeting on Bioimaging for Young Researchers
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research
  • [Presentation] EGF-ERK/MAPKシグナルの反復リレーによる上皮の陥入2017

    • Author(s)
      小椋 陽介, Fu-Lai Wen, Mustafa M. Sami, 柴田 達夫, 林 茂生
    • Organizer
      分子生物学会年会(生命科学系学会合同年次大会)
    • Related Report
      2017 Research-status Report
  • [Presentation] Automated image processing and analysis software for epithelial cells quantification2017

    • Author(s)
      Mustafa M. Sami, Yosuke Ogura, Yu-Chiun Wang, Shigeo Hayashi
    • Organizer
      17th international ELMI meeting
    • Related Report
      2017 Research-status Report
  • [Presentation] Iterative EGF-ERK signaling relay directs mechanical force required for epithelial invagination2017

    • Author(s)
      Yosuke Ogura, Fu-Lai Wen, Mustafa M. Sami, Tatsuo Shibata and Shigeo Hayashi
    • Organizer
      18th international Congress of Developmental Biology
    • Related Report
      2017 Research-status Report
  • [Presentation] An intercellular relay of EGF-ERK signaling polarizes the mechanical force to facilitate invagination of organ placode2017

    • Author(s)
      Yosuke Ogura, Fu-Lai Wen, Mustafa M. Sami, Tatsuo Shibata and Shigeo Hayashi
    • Organizer
      EMBL Symposium: Mechanical Forces in Biology
    • Related Report
      2017 Research-status Report
  • [Presentation] ERK活性の細胞間伝搬が制御する上皮の陥入2016

    • Author(s)
      小椋 陽介, 林 茂生
    • Organizer
      4D細胞計測若手の会
    • Place of Presentation
      KKR熱海
    • Related Report
      2016 Research-status Report

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Published: 2016-04-21   Modified: 2020-03-30  

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