Development of a cooling preservation method that secures the function of retinal tissue for regenerative medicine
| Project/Area Number |
16K21633
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Ophthalmology
Conservation of biological resources
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| Research Institution | Institute of Physical and Chemical Research |
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Principal Investigator |
Koide Naoshi 国立研究開発法人理化学研究所, 生命機能科学研究センター, 研究員 (40714126)
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| Research Collaborator |
SUNAGAWA Genshiro
TSUDA Sakae
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| Project Period (FY) |
2016-04-01 – 2019-03-31
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| Project Status |
Completed (Fiscal Year 2018)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2018: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2017: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2016: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | 再生医療 / 網膜 / 細胞移植 / 細胞保存 / 不凍タンパク質 / 蛍光異方性 / RPE / 網膜組織 / 眼発生・再生医学 / 細胞・組織・種子保存 / 低温 |
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| Outline of Final Research Achievements |
In this research, we worked on development of preservation technology that applied fluorescence anisotropy and antifreeze protein. With regard to fluorescence anisotropy, it was not possible to minimize the light scattering by water due to the thickness problem unique to the three-dimensional structure and the floating state, and it could not be completed. Antifreeze protein was confirmed to improve cell viability by the addition of type III AFP in a refrigerated environment. On the other hand, with regard to cell transport in clinical research conducted in our laboratory, we have reached the determination of transport conditions in clinical research by making use of the know-how acquired in this task. As evidence from scientific evidence, cytotoxicity due to overcooling was also clearly confirmed, and vibration verification was found to be sufficient to provide limited consideration in preparing transport materials at a certain level.
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| Academic Significance and Societal Importance of the Research Achievements |
本研究課題において、蛍光異方性と不凍タンパク質の臨床応用には至らなかったものの、研究開発で得られたノウハウをベースに臨床研究での細胞輸送要件を策定することに成功した。iPS細胞を用いた再生医療臨床研究（RPE他家懸濁液移植）において国内初の細胞輸送工程を実現したことは今後の普及・拡大を考慮した際に重要な前進であったといえる。今回は振動への対応は輸送資材を考慮することで限定的な対応で十分であることが明確となり、温度については細胞ごとに最適化する必要がある知見が得られた。今後は、懸濁液以外のシートや立体成型された構造物などより複雑な特定細胞加工物の輸送にむけて研究開発を促進させる心算である。
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Report
(4 results)
Research Products
(5 results)
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[Journal Article] Knockout-Rescue Embryonic Stem Cell-Derived Mouse Reveals Circadian-Period Control by Quality and Quantity of CRY1.2017
Author(s)
Ode KL, Ukai H, Susaki EA, Narumi R, Matsumoto K, Hara J, Koide N, Abe T, Kanemaki MT, Kiyonari H, Ueda HR.
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Journal Title
Molecular Cell
Volume: 65
Pages: 176-190
Related Report
Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
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