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Quantification of fusion alleles and resistant mutation alleles in plasma of patients with lung cancer

Research Project

Project/Area Number 16K21644
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Respiratory organ internal medicine
Laboratory medicine
Research InstitutionNational Cancer Center Japan

Principal Investigator

Yoshitaka Seki  国立研究開発法人国立がん研究センター, 研究所, 外来研究員 (00733213)

Project Period (FY) 2016-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2017: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywordsリキッドバイオプシー / 血中循環細胞外DNA / ドライバー遺伝子変異 / チロシンキナーゼ阻害薬 / 薬剤耐性診断 / 次世代シークエンシング / 血中循環細胞外DNA(cfDNA)) / 肺がん / チロシンキナーゼ阻害剤 / 耐性化克服 / 癌 / ゲノム / 遺伝子 / 医療・福祉
Outline of Final Research Achievements

Detection of EML4-ALK fusion gene and resistant mutation from genomic DNA is useful for clinical application. We aimed to quantify EML4-ALK fusion alleles and resistant mutation alleles in plasma of patients with lung cancer harboring EML4-ALK fusion.
Our group tried quantitative analysis of EML4-ALK fusion gene in plasma cell-free DNA(cfDNA) using the next-generation sequencer.At first, with the new genomic DNA concentration method, we developed a custom panel including the target sequence mainly on a fused gene and the resistant gene.
Using this technique, every alleles with sequence including the target fusion gene could be quantified, and sequence including breakpoints were identified from cell lines. The quantifiability and reproducibility were also confirmed.

Report

(3 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Research-status Report
  • Research Products

    (4 results)

All 2018 2016 Other

All Journal Article (1 results) (of which Peer Reviewed: 1 results,  Open Access: 1 results) Presentation (2 results) (of which Int'l Joint Research: 1 results) Remarks (1 results)

  • [Journal Article] Circulating cell-free plasma tumour DNA shows a higher incidence of EGFR mutations in patients with extrathoracic disease progression2018

    • Author(s)
      Seki Yoshitaka、Fujiwara Yutaka、Kohno Takashi、Yoshida Kazushi、Goto Yasushi、Horinouchi Hidehito、Kanda Shintaro、Nokihara Hiroshi、Yamamoto Noboru、Kuwano Kazuyoshi、Ohe Yuichiro
    • Journal Title

      ESMO open

      Volume: 3 Issue: 2 Pages: e000292-e000292

    • DOI

      10.1136/esmoopen-2017-000292

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Presentation] 血液中細胞外DNA(cfDNA)からのEGFR 遺伝 子変異検出が可能であった肺腺がん患者の臨床 的背景2016

    • Author(s)
      関 好孝
    • Organizer
      57回日本肺癌学会学術集会 2016年12月19日(月)~21日
    • Place of Presentation
      福岡国際会議場、福岡サンパレス、福岡国際センター(福岡県福岡市)
    • Year and Date
      2016-12-19
    • Related Report
      2016 Research-status Report
  • [Presentation] Analysis of circulating cell-free DNA in plasma shows a higher detection rate of EGFR mutations in patients with extrathoracic disease progression2016

    • Author(s)
      Yoshitaka Seki
    • Organizer
      ESMO 2016 Congress
    • Place of Presentation
      Copenhagen, Denmark
    • Year and Date
      2016-10-07
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research
  • [Remarks] 東京慈恵会医科大学 学術リポジトリ

    • URL

      http://hdl.handle.net/10328/9083

    • Related Report
      2016 Research-status Report

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Published: 2016-04-21   Modified: 2019-03-29  

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