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Identification of transcription factors related to mouse ovarian differentiation and construction of transcription network

Research Project

Project/Area Number 16K21664
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Applied molecular and cellular biology
General anatomy (including histology/embryology)
Research InstitutionTokyo Metropolitan Institute of Medical Science (2017-2018)
National Center for Child Health and Development (2016)

Principal Investigator

KATO Tomoko  公益財団法人東京都医学総合研究所, 生体分子先端研究分野, 研究員 (10638802)

Project Period (FY) 2016-04-01 – 2019-03-31
Project Status Completed (Fiscal Year 2018)
Budget Amount *help
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2017: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2016: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Keywords性分化 / 卵巣 / ノックアウトマウス / 機能解析 / ゲノム編集 / 卵巣分化 / 発現解析 / 卵巣形成 / CRISPR/Cas9 / 生殖腺 / 転写カスケード / 細胞・組織 / 発現制御 / 発生・分化 / 遺伝子
Outline of Final Research Achievements

The key genes involved in fetal mouse ovarian development and molecular mechanism through ovarian differentiation are almost unknown because the ovarian structure is poor during these stages. In this study, we tested to identify the cell type of 18 candidate genes involved in ovarian differentiation by using in situ hybridization and immunostaining. In addition, we generated 18 ovarian genes knock out mice to clarify their functions.

Academic Significance and Societal Importance of the Research Achievements

哺乳類の生殖腺分化過程において、精巣分化過程における分子メカニズムは徐々に明らかになってきているが、卵巣分化過程における分子メカニズムは未だ不明な点が多い、しかし、近年、卵巣分化を引き起こす重要な遺伝子の存在が指摘されてきており、これらの遺伝子は卵巣・卵子の質の低下、及び妊孕性の低下に大きく関わる可能性がある。これらの問題は、女性の社会進出と共に問題視されている高齢出産の壁を解決する糸口となり得ることが期待される。

Report

(4 results)
  • 2018 Annual Research Report   Final Research Report ( PDF )
  • 2017 Research-status Report
  • 2016 Research-status Report
  • Research Products

    (13 results)

All 2018 2017 2016 Other

All Journal Article (9 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 9 results,  Open Access: 9 results,  Acknowledgement Compliant: 2 results) Presentation (3 results) (of which Int'l Joint Research: 1 results) Remarks (1 results)

  • [Journal Article] Mapping of a responsible region for sex reversal upstream of Sox9 by production of mice with serial deletion in a genomic locus.2018

    • Author(s)
      Yuya Ogawa, Miho Terao, Satoshi Hara, Moe Tamano, Haruka Okayasu, Tomoko Kato, Shuji Takada.
    • Journal Title

      Scientific Reports

      Volume: 8 Issue: 1 Pages: 17514-17514

    • DOI

      10.1038/s41598-018-35746-0

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Peptidyl arginine deiminase 2 (Padi2) is expressed in Sertoli cells in a specific manner and regulated by SOX9 during testicular development.2018

    • Author(s)
      Tsuji-Hosokawa A, Kashimada K, Kato T, Ogawa Y, Nomura R, Takasawa K, Lavery R, Coschiera A, Schiessinger D, Harley V, Takada S and Morio T.
    • Journal Title

      Scientific Reports

      Volume: 8 Issue: 1 Pages: 13263-13263

    • DOI

      10.1038/s41598-018-31376-8

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] A tandem repeat array in IG-DMR is essential for imprinting of paternal allele at the Dlk1/Dio3 domain during embryonic development.2018

    • Author(s)
      Saito T, Hara S, Kato T, Tamano M, Muramatsu A, Asahara H and Takada S.
    • Journal Title

      Human Molecular Genetics

      Volume: 27 Issue: 18 Pages: 3283-3292

    • DOI

      10.1093/hmg/ddy235

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 with improved proof-reading enhances homology-directed repair2018

    • Author(s)
      Tomoko Kati-Inui, Gou Takahashi, Szuyin Hsu, Yuichiro Miyaoka
    • Journal Title

      Nucleic Acids Research

      Volume: 1 Issue: 9 Pages: 4677-4688

    • DOI

      10.1093/nar/gky264

    • Related Report
      2018 Annual Research Report 2017 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Creation of mutant mice with megabase-sized deletions containing custom-designed breakpoints by means of the CRISPR/Cas9 system2017

    • Author(s)
      Kato T, Hara S, Goto Y, Ogawa Y, Okayasu H, Kubota S, Tamano M, Terao M, Takada S
    • Journal Title

      Scientific Reports

      Volume: 7 Issue: 1

    • DOI

      10.1038/s41598-017-00140-9

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] CRISPR/Cas9-mediated simultaneous knockout of Dmrt1 and Dmrt3 does not recapitulate the 46,XY gonadal dysgenesis observed in 9p24.3 deletion patients2017

    • Author(s)
      Masafumi Inui, Moe Tamano, Tomoko Kato, Shuji Takada
    • Journal Title

      BB Reports

      Volume: 9 Pages: 238-244

    • DOI

      10.1016/j.bbrep.2017.01.001

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Microinjection-based generation of mutant mice with a double mutation and a 0.5 Mb deletion in their genome by the CRISPR/Cas9 system2016

    • Author(s)
      Hara S, Kato T, Goto Y, Kubota S, Tamano M, Terao M, Takada S
    • Journal Title

      Journal of Reproduction and Development

      Volume: 62 Issue: 5 Pages: 531-536

    • DOI

      10.1262/jrd.2016-058

    • NAID

      130005431273

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Cord Blood-Derived Endothelial Colony-Forming Cell Function is Disrupted in Congenital Diaphragmatic Hernia2016

    • Author(s)
      Hideshi Fujinaga, Hiroko Fujinaga, Nobuyuki Watanabe, Tomoko Kato, Moe Tamano, Miho Terao, Shuji Takada, Yushi Ito, Akihiro Umezawa, Masahiko Kuroda
    • Journal Title

      American Journal of Physiology-Lung Cellular and Molecular Physiology

      Volume: 310 Issue: 11 Pages: 1143-1154

    • DOI

      10.1152/ajplung.00357.2015

    • NAID

      120005845870

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Utilization of the CRISPR/Cas9 system for the efficient production of mutant mice using crRNA/tracrRNA with Cas9 nickase and FokI-dCas92016

    • Author(s)
      Terao M, Tamano M, Hara S, Kato T, Kinoshita M, Takada S
    • Journal Title

      Experimental Animals

      Volume: 65 Issue: 3 Pages: 275-283

    • DOI

      10.1538/expanim.15-0116

    • NAID

      130006882365

    • ISSN
      0007-5124, 1341-1357, 1881-7122
    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] Genome editing via Cas9 RNP improves the HDR/NHEJ ratio2018

    • Author(s)
      Tomoko Kato, Gou Takahashi, Szuyin Hsu, Yuichiro Miyaoka
    • Organizer
      Cold Spring Harbor Laboratory Meeting (Genome Engineering: The CRISPR-Cas Revolution
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Cas9リボ核タンパク質(RNP)を介したゲノム編集はHDR/NHEJ比を亢進する2018

    • Author(s)
      加藤朋子、高橋剛、許絲茵、宮岡佑一郎
    • Organizer
      日本ゲノム編集学会
    • Related Report
      2018 Annual Research Report
  • [Presentation] Cas9とDNAの結合力がHDRとNHEJの活性を規定する2017

    • Author(s)
      加藤朋子、高橋剛、許絲茵、宮岡佑一郎
    • Organizer
      2017年度生命科学系学会合同年次大会(ConBio2017)
    • Related Report
      2017 Research-status Report
  • [Remarks]

    • URL

      https://www.igakuken-regmed.com/

    • Related Report
      2018 Annual Research Report

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Published: 2016-04-21   Modified: 2020-03-30  

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