Identification of transcription factors related to mouse ovarian differentiation and construction of transcription network
| Project/Area Number |
16K21664
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Applied molecular and cellular biology
General anatomy (including histology/embryology)
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| Research Institution | Tokyo Metropolitan Institute of Medical Science (2017-2018)
National Center for Child Health and Development (2016) |
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Principal Investigator |
KATO Tomoko 公益財団法人東京都医学総合研究所, 生体分子先端研究分野, 研究員 (10638802)
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| Project Period (FY) |
2016-04-01 – 2019-03-31
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| Project Status |
Completed (Fiscal Year 2018)
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| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2017: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2016: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Keywords | 性分化 / 卵巣 / ノックアウトマウス / 機能解析 / ゲノム編集 / 卵巣分化 / 発現解析 / 卵巣形成 / CRISPR/Cas9 / 生殖腺 / 転写カスケード / 細胞・組織 / 発現制御 / 発生・分化 / 遺伝子 |
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| Outline of Final Research Achievements |
The key genes involved in fetal mouse ovarian development and molecular mechanism through ovarian differentiation are almost unknown because the ovarian structure is poor during these stages. In this study, we tested to identify the cell type of 18 candidate genes involved in ovarian differentiation by using in situ hybridization and immunostaining. In addition, we generated 18 ovarian genes knock out mice to clarify their functions.
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| Academic Significance and Societal Importance of the Research Achievements |
哺乳類の生殖腺分化過程において、精巣分化過程における分子メカニズムは徐々に明らかになってきているが、卵巣分化過程における分子メカニズムは未だ不明な点が多い、しかし、近年、卵巣分化を引き起こす重要な遺伝子の存在が指摘されてきており、これらの遺伝子は卵巣・卵子の質の低下、及び妊孕性の低下に大きく関わる可能性がある。これらの問題は、女性の社会進出と共に問題視されている高齢出産の壁を解決する糸口となり得ることが期待される。
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Report
(4 results)
Research Products
(13 results)
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[Journal Article] Peptidyl arginine deiminase 2 (Padi2) is expressed in Sertoli cells in a specific manner and regulated by SOX9 during testicular development.2018
Author(s)
Tsuji-Hosokawa A, Kashimada K, Kato T, Ogawa Y, Nomura R, Takasawa K, Lavery R, Coschiera A, Schiessinger D, Harley V, Takada S and Morio T.
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Journal Title
Scientific Reports
Volume: 8
Issue: 1
Pages: 13263-13263
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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