Integrative analysis of metagenomics and metabolomics for eosinophilic gastrointestinal disorders in children

• Project/Area Number: 16K21670
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Pediatrics; Collagenous pathology/Allergology
• Research Institution: National Center for Child Health and Development
• Principal Investigator: Shoda Tetsuo 国立研究開発法人国立成育医療研究センター, 生体防御系内科部, 医師研究員 (00724311)
• Project Period (FY): 2016-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2017: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2016: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 好酸球性消化管疾患 / 腸内細菌叢 / メタボローム / 腸内細菌 / アレルギー・ぜんそく

Outline of Final Research Achievements

Microbes may increase susceptibility to eosinophilic gastrointestinal disorders (EGID) by producing bioactive metabolites that affect immune activity and epithelial function. To uncover disease-associated microbial and metabolomics profile in pediatric EGID by next-generation sequencing analysis, a case-control study (EGID vs control, n=5 vs 10) was conducted. Several analyses, including principal coordinate analysis, cluster analysis and integrated analysis of both microbiome and metabolome, did not show significant differences between EGID and control. In this case-control study, a disease-associated microbial and metabolomics profile in pediatric EGID was not found.

Research Products

• [Journal Article] Serum Biomarkers for the Diagnosis of Eosinophilic Esophagitis and Eosinophilic Gastroenteritis (2017)
  • Author(s): Shunji Ishihara, Tetsuo Shoda, et al.
  • Journal Title: Internal Medicine Volume: 56 Issue: 21 Pages: 2819-2825
  • DOI: 10.2169/internalmedicine.8763-16
  • NAID: 130006191952
  • ISSN: 0918-2918, 1349-7235
  • Peer Reviewed / Open Access
• [Journal Article] Factors Associated with Development of Food Allergy in Young Children after Liver Transplantation: A Retrospective Analysis of 10 Years' Experience. (2017)
  • Author(s): Mitsui M, Shoda T, Natsume O, Nomura I, Narita M, Fukuda A, Sakamoto S, Kasahara M, Ohya Y.
  • Journal Title: J Allergy Clin Immunol Pract. Volume: 5 Issue: 6 Pages: 1698-1706
  • DOI: 10.1016/j.jaip.2017.04.013
  • Peer Reviewed
• [Journal Article] Gene Expression Patterns in Distinct Endoscopic Findings for Eosinophilic Gastritis in Children. (2017)
  • Author(s): Sato M, Shoda T, Shimizu H, Orihara K, Futamura K, Matsuda A, Yamada Y, Irie R, Yoshioka T, Shimizu T, Ohya Y, Nomura I, Matsumoto K, Arai K.
  • Journal Title: The Journal of Allergy and Clinical Immunology: In Practice Volume: 5 Issue: 6 Pages: 1639-1649
  • DOI: 10.1016/j.jaip.2017.03.030
  • Peer Reviewed
• [Journal Article] Yogurt consumption in infancy is inversely associated with atopic dermatitis and food sensitization at 5 years of age: A hospital-based birth cohort study. (2017)
  • Author(s): Shoda T, Futamura M, Yang L, Narita M, Saito H, Ohya Y.
  • Journal Title: J Dermatol Sci Volume: 86 Issue: 2 Pages: 90-6
  • DOI: 10.1016/j.jdermsci.2017.01.006
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Eosinophilic esophagitis versus proton pump inhibitor-responsive esophageal eosinophilia: Transcriptome analysis (2017)
  • Author(s): Shoda T, Matsuda A, Nomura I, Okada N, Orihara K, Mikami H, Ishimura N, Ishihara S, Matsumoto K, Kinoshita Y
  • Journal Title: Journal of Allergy and Clinical Immunology Volume: - Issue: 6 Pages: 2010-2013
  • DOI: 10.1016/j.jaci.2016.11.028
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Factors Associated with Development of Food Allergy in Young Children after Liver Transplantation: A Retrospective Analysis of 10 Years' Experience (2017)
  • Author(s): Mitsui M, Shoda T, Natsume O, Nomura I, Narita M, Fukuda A, Sakamoto S, Kasahara M, Ohya Y
  • Journal Title: J Allergy Clin Immunol Pract Volume: 印刷中
  • Peer Reviewed
• [Journal Article] Gene expression patterns in distinct endoscopic findings for eosinophilic gastritis in children (2017)
  • Author(s): Sato M, Shoda T, Shimizu H, Orihara K, Futamura K, Matsuda A, Yamada Y, Yoshioka T, Shimizu T, Ohya Y, Nomura I, Matsumoto K, Arai K
  • Journal Title: J Allergy Clin Immunol Pract Volume: 印刷中
  • Peer Reviewed
• [Journal Article] Serum biomarkers for diagnosis of eosinophilic esophagitis and eosinophilic gastroenteritis. (2017)
  • Author(s): Ishihara S, Shoda T, Ishimura N, Ohta S, Ono J, Azuma Y, Okimoto E, Izuhara K, Nomura I, Matsumoto K, Kinoshita Y
  • Journal Title: Intern Med Volume: 印刷中
  • NAID: 130006191952
  • Peer Reviewed
• [Journal Article] 好酸球性食道炎の分子機構-食道での発現蛋白解析データを中心に- (2016)
  • Author(s): 正田哲雄
  • Journal Title: G.I.Research Volume: 24 Pages: 175-180