| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2016: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Outline of Final Research Achievements |
In this study, risk assessment of 4 types of benzotriazole ultraviolet stabilizers (BUVSs) which show AhR ligand activities was attempted. First, oxidation of BUVSs by 8 types of major cytochrome P450s and changes in AhR ligand activity caused by oxidation were evaluated. As a result, BUVSs were oxidized by both AhR-dependent and independent cytochrome P450s, then, lose their original AhR ligand activity. It means that BUVSs would be categorized "no risk AhR ligand". Metabolite search was also conducted using LC-MS/MS, then UV-P and UV-9 confirmed the presence of oxidative metabolites. In addition, when the DNA damage property of BUVSs was evaluated using umu test, all BUVSs except for UV-326 were negative. From above, BUVSs other than UV-326 were considered as harmless low-risk AhR ligands. In the case of UV-326, it showed nearly positive response by umu test, therefore, comprehensive genotoxicity assessment were required.
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