| Outline of Final Research Achievements |
In this study, we analyzed the subcellular localization of Na+/H+- anti-transporters (NHX5 and NHX6) during salt stress response. The results suggest that NHX5 localizes mainly to MVEs, and functions in the vacuolar transport pathway. Interestingly, salt stress treatment decreased the percentage of NHX5-GFP dot-like structures that colocalized with the TGN. Conversely, salt stress treatment significantly increased the percentage of NHX5 dot-like structures that co-localized with MVEs. These results suggest that Na+ storage in the membrane structures such as TGNs, MVEs, and novel vesicles, is important in the salt stress response.
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