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Comprehensive analysis of mRNA alternative splicing patterns in barley seeds(Fostering Joint International Research)

Research Project

Project/Area Number 16KK0160
Research Category

Fund for the Promotion of Joint International Research (Fostering Joint International Research)

Allocation TypeMulti-year Fund
Research Field Plant molecular biology/Plant physiology
Research InstitutionTokyo University of Agriculture and Technology

Principal Investigator

Umezawa Taishi  東京農工大学, (連合)農学研究科(研究院), 教授 (70342756)

Project Period (FY) 2017 – 2019
Project Status Completed (Fiscal Year 2019)
Budget Amount *help
¥12,740,000 (Direct Cost: ¥9,800,000、Indirect Cost: ¥2,940,000)
Keywordsオオムギ / 種子休眠 / 後熟 / アブシシン酸 / 選択的スプライシング / プロテオーム / リン酸化 / 穂発芽 / スプライシング
Outline of Final Research Achievements

Preharvest sprouting of cereals, like barley or wheat, is one of the most significant problems in agricultural production worldwide. In general, seed germination depends on seed dormancy, which is mainly regulated by a phytohormone abscisic acid (ABA). Barley seeds have a strong seed dormancy just after harvesting, but its dormancy level is gradually impaired during after-ripening process. In this project, we aim to understand what happens in barley seeds during after-ripening process, in collaboration with a research group of CSIRO, Australia. We performed comparative phosphoproteomic analysis and RNA-seq analysis using two barley seeds with different seed dormancy level, to display ABA signaling events and transcriptional regulation during after-ripening process.

Academic Significance and Societal Importance of the Research Achievements

本研究で行ったオオムギ種子のリン酸化プロテオーム解析によって、オオムギ種子におけるリン酸化タンパク質のデータをこれまでにない規模で収集することに成功した。したがって、種子休眠以外の様々な研究にも有用なデータとなることが期待される。また、これまで種子休眠の研究は遺伝子発現のレベルで行うことが多かったが、本研究ではRNA-seq解析のデータから選択的スプライシングを検出している点が特徴となっている。
オオムギをはじめとする穀物種子の休眠性は、穂発芽等の農業上の問題に関わってくるため重要である。本研究によって、オオムギの種子休眠機構の理解が進めば、将来的に穂発芽の被害軽減につながる可能性がある。

Report

(2 results)
  • 2019 Annual Research Report   Final Research Report ( PDF )
  • Research Products

    (13 results)

All 2020 2019 2018

All Int'l Joint Research (1 results) Journal Article (5 results) (of which Int'l Joint Research: 4 results,  Peer Reviewed: 5 results,  Open Access: 3 results) Presentation (7 results)

  • [Int'l Joint Research] オーストラリア連邦科学産業研究機構(オーストラリア)2018

    • Related Report
      2019 Annual Research Report
  • [Journal Article] Arabidopsis Raf‐like kinases act as positive regulators of subclass III SnRK2 in osmostress signaling2020

    • Author(s)
      Shohei Katsuta 、Goro Masuda、Hyeokjin Bak、Akihisa Shinozawa、Yoshiaki Kamiyama、Taishi Umezawa、Daisuke Takezawa、Izumi Yotsui、Teruaki Taji、Yoichi Sakata
    • Journal Title

      The Plant Journal

      Volume: 324 Issue: 2 Pages: 1064-1064

    • DOI

      10.1111/tpj.14756

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] A role for PM19-Like 1 in seed dormancy in Arabidopsis2019

    • Author(s)
      Barrero Jose M.、Dorr Marie M.、Talbot Mark J.、Ishikawa Shinnosuke、Umezawa Taishi、White Rosemary G.、Gubler Frank
    • Journal Title

      Seed Science Research

      Volume: 29 Issue: 3 Pages: 184-196

    • DOI

      10.1017/s0960258519000151

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Comparative Phosphoproteomic Analysis Reveals a Decay of ABA Signaling in Barley Embryos during After-Ripening2019

    • Author(s)
      Ishikawa Shinnosuke、Barrero Jose M、Takahashi Fuminori、Nakagami Hirofumi、Peck Scott C、Gubler Frank、Shinozaki Kazuo、Umezawa Taishi
    • Journal Title

      Plant and Cell Physiology

      Volume: 60 Issue: 12 Pages: 2758-2768

    • DOI

      10.1093/pcp/pcz163

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] SnRK2 protein kinases represent an ancient system in plants for adaptation to a terrestrial environment2019

    • Author(s)
      Shinozawa Akihisa、Otake Ryoko、Takezawa Daisuke、Umezawa Taishi、Komatsu Kenji、Tanaka Keisuke、Amagai Anna、Ishikawa Shinnosuke、Hara Yurie、Kamisugi Yasuko、Cuming Andrew C.、Hori Koichi、Ohta Hiroyuki、Takahashi Fuminori、Shinozaki Kazuo、Hayashi Takahisa、Taji Teruaki、Sakata Yoichi
    • Journal Title

      Communications Biology

      Volume: 2 Issue: 1 Pages: 30-30

    • DOI

      10.1038/s42003-019-0281-1

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Comparative Phosphoproteomic Analysis of Barley Embryos with Different Dormancy during Imbibition2019

    • Author(s)
      Ishikawa S, Barrero J, Takahashi F, Peck S, Gubler F, Shinozaki K, Umezawa T.
    • Journal Title

      International Journal of Molecular Sciences

      Volume: 20 Issue: 2 Pages: 451-451

    • DOI

      10.3390/ijms20020451

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Presentation] アブシジン酸応答に関わるグループC Raf型タンパク質リン酸化酵素の機能解析2019

    • Author(s)
      神山佳明、廣谷美咲、峯岸芙有子、野元美佳、多田安臣、坂田洋一、竹澤大輔、Scott Peck、梅澤泰史
    • Organizer
      日本植物学会第83回大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] SnRK2-substrate 1は植物の開花期制御に関与する2019

    • Author(s)
      片桐壮太郎、鈴木梨沙、飯泉沙良、石塚梢、神山佳明、梅澤泰史
    • Organizer
      日本植物学会第83回大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] アブシジン酸応答に関与するSnRK2プロテインキナーゼの活性阻害剤のスクリーニング2019

    • Author(s)
      松岡頌子、佐藤花綸、今村理世、能年義輝、岡部隆義、梅澤泰史
    • Organizer
      日本植物学会第83回大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] ABAシグナル伝達の主要経路に関わるグループC Raf型タンパク質リン酸化酵素の機能解析2019

    • Author(s)
      神山佳明、廣谷美咲、峯岸芙有子、野元美佳、多田安臣、坂田洋一、竹澤大輔、Scott Peck、梅澤泰史
    • Organizer
      日本植物生理学会第60回年会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 後熟期におけるオオムギ種子胚の比較リン酸化プロテオーム解析2019

    • Author(s)
      "石川慎之祐,Jose Barrero,高橋史憲,中神弘史,Scott Peck,Frank Gubler,篠崎一雄,梅澤 泰史"
    • Organizer
      日本植物生理学会第60回年会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 開花時期の調節におけるSnRK2 substrate1 の機能解析2019

    • Author(s)
      片桐壮太郎,鈴木梨沙,神山佳明,梅澤泰史
    • Organizer
      日本植物生理学会第60回年会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 低分子化合物スクリーニングによるSnRK2プロテインキナーゼ活性化剤の探索2019

    • Author(s)
      松岡頌子,佐藤花綸,今村理世,能年義輝,岡部隆義,梅澤泰史
    • Organizer
      日本植物生理学会第60回年会
    • Related Report
      2019 Annual Research Report

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Published: 2017-03-15   Modified: 2021-02-19  

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