Outline of Final Research Achievements |
Preharvest sprouting of cereals, like barley or wheat, is one of the most significant problems in agricultural production worldwide. In general, seed germination depends on seed dormancy, which is mainly regulated by a phytohormone abscisic acid (ABA). Barley seeds have a strong seed dormancy just after harvesting, but its dormancy level is gradually impaired during after-ripening process. In this project, we aim to understand what happens in barley seeds during after-ripening process, in collaboration with a research group of CSIRO, Australia. We performed comparative phosphoproteomic analysis and RNA-seq analysis using two barley seeds with different seed dormancy level, to display ABA signaling events and transcriptional regulation during after-ripening process.
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