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Epigenome analysis in the commercial F1 hybrid cultivar in Chinese cabbage(Fostering Joint International Research)

Research Project

Project/Area Number 16KK0171
Research Category

Fund for the Promotion of Joint International Research (Fostering Joint International Research)

Allocation TypeMulti-year Fund
Research Field Horticultural science
Research InstitutionKobe University

Principal Investigator

Fujimoto Ryo  神戸大学, 農学研究科, 准教授 (60620375)

Project Period (FY) 2017 – 2022
Project Status Completed (Fiscal Year 2022)
Budget Amount *help
¥14,040,000 (Direct Cost: ¥10,800,000、Indirect Cost: ¥3,240,000)
Keywordsヘテロシス / エピジェネティクス / トランスクリプトーム / アブラナ科 / 育種学 / 園芸学 / 遺伝学 / ゲノム
Outline of Final Research Achievements

Epigenome analysis was performed to elucidate the molecular mechanisms of hybrid vigor in Chinese cabbage. Whole genome DNA methylation analysis revealed that DNA methylation in the upstream and downstream 200 bp of the gene was negatively associated with expression level. The repressive histone mark, H3K27me3, was shown to potentially play an important role in the regulation of tissue-specific gene expression. Genes with active histone modifications, H3K4me3 or H3K36me3, tended to have a higher expression level. We also identified genes having both the active H3K4me3 and repressive H3K27me3 modifications. We showed that histone modification levels in F1 were close to those of either parent.

Academic Significance and Societal Importance of the Research Achievements

Brassica rapa種において、ヒストン修飾を対象としたエピゲノム解析は実施されていなかった。本研究によりB. rapaにおいて、ヒストン修飾が遺伝子発現に果たす役割を明らかにできた。両親系統とF1の比較解析から、F1では親のエピゲノム状態が維持されていることを明らかにした。以上より、本成果は学術的な意義があると考える。今後、遺伝学的な研究とエピゲノム情報を統合して解析を進めていくことで雑種強勢に重要な遺伝領域を明らかにする計画で、一代雑種育種の省力化に繋がる発展性が期待できることから、本研究の社会的な意義は大きいと考える。

Report

(2 results)
  • 2022 Annual Research Report   Final Research Report ( PDF )
  • Research Products

    (17 results)

All 2023 2022 2018

All Int'l Joint Research (1 results) Journal Article (4 results) (of which Int'l Joint Research: 4 results,  Peer Reviewed: 4 results,  Open Access: 2 results) Presentation (11 results) (of which Int'l Joint Research: 6 results,  Invited: 1 results) Book (1 results)

  • [Int'l Joint Research] CSIRO(オーストラリア)2018

    • Related Report
      2022 Annual Research Report
  • [Journal Article] Studies on the Molecular Basis of Heterosis in Arabidopsis thaliana and Vegetable Crops2023

    • Author(s)
      Shiraki Saaya、Fujiwara Kazumasa、Kamiya Yoshiki、Akter Mst. Arjina、Dennis Elizabeth S.、Fujimoto Ryo、Mehraj Hasan
    • Journal Title

      Horticulturae

      Volume: 9 Issue: 3 Pages: 366-366

    • DOI

      10.3390/horticulturae9030366

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] The role of histone modification in gene expression in <i>Brassica rapa</i> vegetables2023

    • Author(s)
      Shiraki S.、Kamiya Y.、Mehraj H.、Takahashi S.、Seki M.、Dennis E.S.、Fujimoto R.
    • Journal Title

      Acta Horticulturae

      Volume: 1362 Issue: 1362 Pages: 107-112

    • DOI

      10.17660/actahortic.2023.1362.15

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] The role of epigenetic modifications in the transcriptional regulation of long noncoding RNAs in <i>Brassica rapa</i> vegetables2023

    • Author(s)
      Kamiya Y.、Shiraki S.、Mehraj H.、Akter M.A.、Takahashi S.、Seki M.、Dennis E.S.、Osabe K.、Fujimoto R.
    • Journal Title

      Acta Horticulturae

      Volume: 1362 Issue: 1362 Pages: 65-70

    • DOI

      10.17660/actahortic.2023.1362.10

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Will epigenetics be a key player in crop breeding?2022

    • Author(s)
      Tonosaki Kaoru、Fujimoto Ryo、Dennis Elizabeth S.、Raboy Victor、Osabe Kenji
    • Journal Title

      Frontiers in Plant Science

      Volume: 13 Pages: 958350-958350

    • DOI

      10.3389/fpls.2022.958350

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Presentation] Brassica rapaにおけるH3K4me3とH3K36me3の遺伝子発現における役割2023

    • Author(s)
      白木沙彩、Mehraj Hasan、安田剛志、藤本龍
    • Organizer
      園芸学会令和5年度春季大会
    • Related Report
      2022 Annual Research Report
  • [Presentation] シロイヌナズナの雑種強勢に関係する遺伝領域の同定2022

    • Author(s)
      藤原一将、宮路直実、安田剛志、藤本龍
    • Organizer
      日本育種学会第142回講演会
    • Related Report
      2022 Annual Research Report
  • [Presentation] The role of histone modification in gene expression in Brassica rapa vegetables2022

    • Author(s)
      Saaya Shiraki, Hasan Mehraj, Satoshi Takahashi, Ayasha Akter, Naomi Miyaji, Motoaki Seki, Elizabeth S. Dennis, Ryo Fujimoto
    • Organizer
      IHC2022
    • Related Report
      2022 Annual Research Report
    • Int'l Joint Research
  • [Presentation] ハクサイにおいて活性型ヒストン修飾が遺伝子発現に及ぼす影響2022

    • Author(s)
      白木沙彩、Mehraj Hasan、高橋聡史、安田剛志、関原明、藤本龍
    • Organizer
      園芸学会令和4年度秋季大会
    • Related Report
      2022 Annual Research Report
  • [Presentation] Identification of the genetic region related to heterosis in Arabidopsis thaliana2022

    • Author(s)
      Kazumasa Fujiwara, Naomi Miyaji, Satoshi Takahashi, Motoki Shimizu, Motoaki Seki, Takeshi Yasuda, Elizabeth S. Dennis, Ryo Fujimoto
    • Organizer
      Cold Spring Harbor Asia conference on Integrative Epigenetics in Plants
    • Related Report
      2022 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Change of trimethylation of lysine 27 of histone H3 in the decrease in DNA methylation 1 mutant of Arabidopsis thaliana2022

    • Author(s)
      Saaya Shiraki, Kodai Matsuo, Satoshi Takahashi, Motoki Shimizu, Elizabeth S. Dennis, Motoaki Seki, Ryo Fujimoto
    • Organizer
      Cold Spring Harbor Asia conference on Integrative Epigenetics in Plants
    • Related Report
      2022 Annual Research Report
  • [Presentation] The epigenome analysis in Brassica rapa L.2022

    • Author(s)
      Ryo Fujimoto
    • Organizer
      School of Life and Medical Sciences Research Conference
    • Related Report
      2022 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Role of four major histone midifications in regulating heterosis in Chinease cabbage2022

    • Author(s)
      Hasan Mehraj, Satoshi Takahashim Motoki Shimizu, Takeshi Yasuda, Elizabeth S. Dennis, Motoaki Seki, Ryo Fujimoto
    • Organizer
      Cold Spring Harbor Asia conference on Integrative Epigenetics in Plants
    • Related Report
      2022 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Genetic and epigenetic approaches for understanding the molecular mechanism of heterosis in Arabidopsis thaliana and Brassica rapa2022

    • Author(s)
      Ryo Fujimoto, Hasan Mehraj, Satoshi Takahashi, Kazumasa Fujiwara, Naomi Miyaji, Saaya Shiraki, Motoki Shimizu, Motoaki Seki, Elizabeth S. Dennis
    • Organizer
      Cold Spring Harbor Asia conference on Integrative Epigenetics in Plants
    • Related Report
      2022 Annual Research Report
    • Int'l Joint Research
  • [Presentation] シロイヌナズナの耐塩性における雑種強勢の評価2022

    • Author(s)
      神矢良樹、Mehraj Hasan、安田剛志、藤本龍
    • Organizer
      日本育種学会第142回講演会
    • Related Report
      2022 Annual Research Report
  • [Presentation] The relationship between the function of DECREASE IN DNA METHYLATION 1 (DDM1) and the salinity tolerance of Arabidopsis thaliana accessions, Columbia-0 and C242022

    • Author(s)
      Yoshiki Kamiya, Hasan Mehraj, Kodai Matsuo, Motoki Shimizu, Satoshi Takahashi, Motoaki Seki, Takeshi Takasaki, Ryo Fujimoto
    • Organizer
      Cold Spring Harbor Asia conference on Integrative Epigenetics in Plants
    • Related Report
      2022 Annual Research Report
    • Int'l Joint Research
  • [Book] Smart Plant Breeding for Vegetable Crops in Post-genomics Era2023

    • Author(s)
      Yoshiki Kamiya, Saaya Shiraki, Kazumasa Fujiwara, Mst. Arjina Akter, Ayasha Akter, Ryo Fujimoto, Hasan Mehraj
    • Total Pages
      426
    • Publisher
      Springer, Singapore
    • Related Report
      2022 Annual Research Report

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Published: 2017-03-15   Modified: 2025-01-30  

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