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Analysis of osteoclast precursor niche regulated by mesenchymal stem cells(Fostering Joint International Research)

Research Project

Project/Area Number 16KK0190
Research Category

Fund for the Promotion of Joint International Research (Fostering Joint International Research)

Allocation TypeMulti-year Fund
Research Field Functional basic dentistry
Research InstitutionTokyo Dental College (2019)
Matsumoto Dental University (2016-2017)

Principal Investigator

Mizoguchi Toshihide  東京歯科大学, 歯学部, 准教授 (90329475)

Project Period (FY) 2017 – 2019
Project Status Completed (Fiscal Year 2019)
Budget Amount *help
¥14,560,000 (Direct Cost: ¥11,200,000、Indirect Cost: ¥3,360,000)
Keywords破骨細胞 / 破骨細胞前駆細胞 / ニッチ / 骨髄間葉系幹細胞 / 1細胞解析 / RANKL / PTH / Nestin-GFP / 間葉系幹細胞 / シングルセルアッセイ / イメージング解析
Outline of Final Research Achievements

In this project, we tried to understand the regulatory mechanism QOP differentiation identified as an osteoclast precursor in vivo. We hypothesized that bone marrow mesenchymal stem cells (BM-MSC) support the QOP as their microenvironment (niche), and performed characterization of the BM-MSC. We examined effects of parathyroid hormone (PTH)(1-34), a medicine for treatment of osteoporosis, on lineage differentiation of BM-MSC. We found that the contribution of BM-MSC into osteoblasts was accelerated in response to PTH (1-34) treatment (Sci Rep 7:4928, 2017). The PTH(1-34) decreased BM adipocytes, indicating that PTH(1-34) treatment recover osteoporotic bone tissue by skewing the lineage differentiation of BM-MSC toward osteoblasts from adipocytes (J Bone Miner Res 34:1952, 2019).

Academic Significance and Societal Importance of the Research Achievements

本研究成果より、骨粗鬆症の治療薬がBM-MSCの分化制御を介して作用することが明らかになった。BM-MSCの分化制御については不明な点が未だ多く、本研究から得られた成果は高い学術的な意義を包含する。また、本邦が直面している超高齢化社会において、骨粗鬆症の発症および治療効果のメカニズムの理解は急務であることから、本研究で得られた知見は社会的意義があると考える。

Report

(2 results)
  • 2019 Annual Research Report   Final Research Report ( PDF )
  • Research Products

    (21 results)

All 2019 2018 2017

All Int'l Joint Research (1 results) Journal Article (3 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 3 results,  Open Access: 2 results) Presentation (17 results) (of which Int'l Joint Research: 3 results,  Invited: 8 results)

  • [Int'l Joint Research] Albert Einstein College of Medicine(米国)2017

    • Related Report
      2019 Annual Research Report
  • [Journal Article] Parathyroid Hormone Shifts Cell Fate of a Leptin Receptor-Marked Stromal Population from Adipogenic to Osteoblastic Lineage.2019

    • Author(s)
      Yang M, Arai A, Udagawa N, Zhao L, Nishida D, Murakami K, Hiraga T, Takao-Kawabata R, Matsuo K, Komori T, Kobayashi Y, Takahashi N, Isogai Y, Ishizuya T, Yamaguchi A, Mizoguchi T
    • Journal Title

      J Bone Miner Res

      Volume: 34 Issue: 10 Pages: 1952-1963

    • DOI

      10.1002/jbmr.3811

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] 硬組織維持に働く幹細胞の新たな研究手法と最近の知見2019

    • Author(s)
      溝口利英
    • Journal Title

      日本歯科医師会雑誌

      Volume: 72 Pages: 17-25

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Bone Marrow Cells Inhibit BMP-2-Induced Osteoblast Activity in the Marrow Environment2018

    • Author(s)
      Nguyen Ha Thi、Ono Mitsuaki、Oida Yasutaka、Hara Emilio Satoshi、Komori Taishi、Akiyama Kentaro、Nguyen Ha Thi Thu、Aung Kyaw Thu、Pham Hai Thanh、Tosa Ikue、Takarada Takeshi、Matsuo Koichi、Mizoguchi Toshihide、Oohashi Toshitaka、Kuboki Takuo
    • Journal Title

      Journal of Bone and Mineral Research

      Volume: 34 Issue: 2 Pages: 327-332

    • DOI

      10.1002/jbmr.3598

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Presentation] 遺伝子改変マウスを用いた硬組織イメージング解析2019

    • Author(s)
      溝口利英
    • Organizer
      第425回北海道歯学会例会
    • Related Report
      2019 Annual Research Report
    • Invited
  • [Presentation] 遺伝子改変マウスによるイメージング手法を用いた硬組織研究2019

    • Author(s)
      溝口利英
    • Organizer
      第71回再生医療カンファランス
    • Related Report
      2019 Annual Research Report
    • Invited
  • [Presentation] 歯と骨の幹細胞の話2019

    • Author(s)
      溝口利英
    • Organizer
      インプラント研究会
    • Related Report
      2019 Annual Research Report
    • Invited
  • [Presentation] 遺伝子改変マウスを用いた形態学的硬組織研究2019

    • Author(s)
      溝口利英
    • Organizer
      第124回日本解剖学会総会全国学術集会
    • Related Report
      2019 Annual Research Report
    • Invited
  • [Presentation] Hard tissue imaging analysis using genetically modified mice2019

    • Author(s)
      Toshihide Mizoguchi
    • Organizer
      The7th Seoul Symposium on Bone Health in conjunction with the 31th Spring Scientific Congress of Korean Society for Bone and Mineral Research
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Maintenance of hard tissue homeostasis by mesenchymal stem cells2019

    • Author(s)
      Toshihide Mizoguchi
    • Organizer
      The16th Meeting of Bone Biology Forum
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 間葉系幹細胞が司る硬組織維持機構の解析2019

    • Author(s)
      溝口利英
    • Organizer
      第61回歯科基礎医学会学術大会
    • Related Report
      2019 Annual Research Report
    • Invited
  • [Presentation] In vivo Dynamics of Mesenchymal Stem Cells Maintaining Hard Tissue Homeostasis2019

    • Author(s)
      Toshihide Mizoguchi
    • Organizer
      Japan Bone Academy
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 歯髄における破歯細胞抑制メカニズムの解析2019

    • Author(s)
      西田大輔, 荒井敦, 宇田川信之, 中村美どり, 堀部寛治, 小林泰浩, 高橋直之, 溝口利英
    • Organizer
      第4回日本骨免疫学会ウインターセミナー
    • Related Report
      2019 Annual Research Report
  • [Presentation] 象牙芽細胞の枯渇は修復象牙質を誘導する2019

    • Author(s)
      趙麗娟, 荒井敦, 堀部寛治, 細矢明宏, 増子倫也, 小林泰浩, 宇田川信之, 高橋直之, 李憲起, 各務秀明, 溝口利英
    • Organizer
      第4回日本骨免疫学会ウインターセミナー
    • Related Report
      2019 Annual Research Report
  • [Presentation] Depletion of odontoblasts induces reparative dentin formation2019

    • Author(s)
      Zhao L., Arai A., Udagawa N., Horibe K., Hosoya A., Masuko R., Okabe K., Shin M., Kobayashi Y., Takahashi N., Li X., Kagami H., Mizoguchi T.
    • Organizer
      The 7th Seoul Symposium on Bone Health in conjunction with the 31th Spring Scientific Congress of Korean Society for Bone and Mineral Research
    • Related Report
      2019 Annual Research Report
  • [Presentation] Gli1 陽性歯根膜細胞は幹細胞特性を有し,歯槽骨再生に寄与する2019

    • Author(s)
      Shakehin Nazmus,細矢明宏,建部廣明,溝口利英,吉羽永子,吉羽邦彦,中村浩彰,HASAN Md Riasat,入江一元
    • Organizer
      第61回歯科基礎医学会学術大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 象牙芽細胞の枯渇は象牙質形成を誘導する2019

    • Author(s)
      趙麗娟,荒井敦,宇田川信之,細矢明宏,岡部幸司,進正史,李憲起,小林泰浩,高橋直之, 溝口利英
    • Organizer
      第61回歯科基礎医学会学術大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 破歯/破骨細胞形成を負に制御する歯髄環境の解析2019

    • Author(s)
      西田大輔,荒井敦,宇田川信之,中村美どり,堀部寛治,小林泰浩,高橋直之,溝口利英
    • Organizer
      第61回歯科基礎医学会学術大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] チャネルキナーゼTRPM7 の骨格形成における発現と軟骨形成制御2019

    • Author(s)
      進正史,森志穂美,溝口利英,岡本富士雄,鍛治屋浩,荒井敦,宇田川信之,岡部幸司
    • Organizer
      第37回日本骨代謝学会学術集会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 象牙芽細胞の枯渇は象牙芽細胞の分化と石灰化を誘導する2019

    • Author(s)
      溝口利英
    • Organizer
      第4回Skeletal Science Retreat
    • Related Report
      2019 Annual Research Report
  • [Presentation] 破歯/破骨細胞形成を負に制御する歯髄環境の解析2019

    • Author(s)
      西田大輔, 荒井敦, 宇田川信之, 中村美どり, 堀部寛治, 小林泰浩, 高橋直之, 溝口利英
    • Organizer
      第5回日本骨免疫学会ウインタースクール
    • Related Report
      2019 Annual Research Report

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Published: 2017-03-15   Modified: 2024-01-30  

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