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Sustainable control of pathogens and beneficial endophytes with tryptophan-derived metabolism in plants

Research Project

Project/Area Number 16KT0031
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeMulti-year Fund
Section特設分野
Research Field Food Cycle Research
Research InstitutionNara Institute of Science and Technology

Principal Investigator

Saijo Yusuke  奈良先端科学技術大学院大学, 先端科学技術研究科, 准教授 (50587764)

Co-Investigator(Kenkyū-buntansha) 中尾 佳亮  京都大学, 工学研究科, 教授 (60346088)
藤田 美紀  国立研究開発法人理化学研究所, 環境資源科学研究センター, 研究員 (70332294)
Research Collaborator HIRUMA kei  
Project Period (FY) 2016-07-19 – 2019-03-31
Project Status Completed (Fiscal Year 2018)
Budget Amount *help
¥18,330,000 (Direct Cost: ¥14,100,000、Indirect Cost: ¥4,230,000)
Fiscal Year 2018: ¥6,110,000 (Direct Cost: ¥4,700,000、Indirect Cost: ¥1,410,000)
Fiscal Year 2017: ¥7,930,000 (Direct Cost: ¥6,100,000、Indirect Cost: ¥1,830,000)
Fiscal Year 2016: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Keywords共生 / 植物 / 微生物 / 二次代謝 / 病害抵抗性 / 植物微生物相互作用 / 化学合成 / 植物免疫 / 内生菌 / 環境調和型植物生産 / 内生菌・共生菌 / 植物成長・生理 / 植物分子生理学 / 植物代謝調節 / 生体分子 / 有機化学
Outline of Final Research Achievements

To exploit beneficial plant-inhabiting microbes and repel plant pathogens, we investigate into the mechanisms by which plants accommodate pathogens without discernible disease symptoms. We reveal the endophytic fungus Colletotrichum spp (CgE) from radish roots, which colonize and protects the host from pathogens' infection and diseases. Co-inoculation of CgE restricts the growth of different root-infecting pathogenic fungi in Brassicaceae plants. Genetic studies in Arabidopsis thaliana indicate a critical role for ethylene-mediated defenses and tryptophan-derived antimicrobial metabolites of the host in CgE-mediated plant protection. We also reveal several host metabolites, of which exogenous application is effective in the proper control of CgE and host protection. Moreover, comparative genomics studies between CgE and its pathogenic relatives and transcriptome profiling of CgE-colonized plants gain important insight into the basis for endophyte-mediated plant protection.

Academic Significance and Societal Importance of the Research Achievements

持続的な食料生産技術の開発は世界的に喫緊の課題である。中でも、植物生産に甚大な被害を与える病原糸状菌(カビ)に対する作物保護技術を確立すること、並びに環境に低負荷で植物生産の増加を図ることは極めて重要な位置付けにある。植物が体表や組織内に病気を起こさずに宿している多様な共棲菌には植物の成長補助や生体防御に貢献するものが知られ、低肥料・持続型農業のシーズとして高い期待を集めている。したがって、上記の成果をベースに、一見相反する「病原糸状菌の防除」と「有用共棲菌の活用」を両立する技術の開発を推進することの意義は大きい。また、有効性が示された研究手法も他の系に活用が可能で、波及効果も期待できる。

Report

(4 results)
  • 2018 Annual Research Report   Final Research Report ( PDF )
  • 2017 Research-status Report
  • 2016 Research-status Report
  • Research Products

    (18 results)

All 2019 2018 2017 2016 Other

All Int'l Joint Research (1 results) Journal Article (7 results) (of which Int'l Joint Research: 3 results,  Peer Reviewed: 7 results,  Open Access: 4 results,  Acknowledgement Compliant: 2 results) Presentation (9 results) (of which Int'l Joint Research: 1 results,  Invited: 3 results) Book (1 results)

  • [Int'l Joint Research] マックスプランク植物育種科学研究所(ドイツ)

    • Related Report
      2016 Research-status Report
  • [Journal Article] Pattern recognition receptors and signaling in plant-microbe interactions.2018

    • Author(s)
      Saijo Y, Loo EP, and Yasuda S.
    • Journal Title

      Plant J

      Volume: 93 Issue: 4 Pages: 592-613

    • DOI

      10.1111/tpj.13808

    • Related Report
      2018 Annual Research Report 2017 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Identifying the target genes of SUPPRESSOR OF GAMMA RESPONSE 1, a master transcription factor controlling DNA damage response in Arabidopsis.2018

    • Author(s)
      Ogita N, Okushima Y, Tokizawa M, Yamamoto YY, Tanaka M, Seki M, Makita Y, Matsui M, Okamoto-Yoshiyama K, Sakamoto T, Kurata T, Hiruma K, Saijo Y, Takahashi N, Umeda M.
    • Journal Title

      The Plant Journal

      Volume: - Issue: 3 Pages: 439-453

    • DOI

      10.1111/tpj.13866

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] A look at plant immunity through the window of the multitasking coreceptor BAK1.2017

    • Author(s)
      Yasuda S, Okada K, Saijo Y
    • Journal Title

      Current Opinion in Plant Biology

      Volume: 38 Pages: 10-18

    • DOI

      10.1016/j.pbi.2017.04.007

    • Related Report
      2017 Research-status Report 2016 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] NLR locus-mediated trade-off between abiotic and biotic stress adaptation in Arabidopsis2017

    • Author(s)
      Ariga Hirotaka、Katori Taku、Tsuchimatsu Takashi、Hirase Taishi et al.
    • Journal Title

      Nature Plants

      Volume: 3 Issue: 6 Pages: 17072-17072

    • DOI

      10.1038/nplants.2017.72

    • NAID

      120006309713

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Host immunity-mediated control of endophytic/symbiotic microbes in plants.2017

    • Author(s)
      Lee, T.-H., Kitagawa, N., Hiruma, K. Saijo, Y.
    • Journal Title

      Journal of Environmental Biotechnology

      Volume: 17

    • Related Report
      2016 Research-status Report
    • Peer Reviewed
  • [Journal Article] Epigenetic control of defense signaling and priming in plants2016

    • Author(s)
      Espinas NA, Saze H, Saijo Y
    • Journal Title

      Frontiers in Plant Science

      Volume: 7 Pages: 1201-1201

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Regulation of sugar transporter activity for antibacterial defense in Arabidopsis2016

    • Author(s)
      Yamada K, Saijo Y, Nakagami H, Takano Y
    • Journal Title

      Science

      Volume: 354(6318) Issue: 6318 Pages: 1427-1430

    • DOI

      10.1126/science.aah5692

    • NAID

      120005895801

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Presentation] Endophyte-mediated control of fungal pathogenesis in Arabidopsis thaliana2019

    • Author(s)
      Pathompitaknukul K, Hiruma K and Saijo Y
    • Organizer
      平成31年日本植物病理学会大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 糸状菌 Colletotrichum tofieldiae の種内比較から明らかになった Colletotrichum属菌の多様な宿主感染様式2018

    • Author(s)
      晝間 敬、杉田 亮平、田野井 慶太朗、佐藤 豊三、西條 雄介
    • Organizer
      平成30年度日本植物病理学会大会
    • Related Report
      2017 Research-status Report
  • [Presentation] Colletotrichum endophyte-mediated control of fungal pathogenesis in Arabidopsis thaliana2018

    • Author(s)
      Kuldanai Pathompitaknukul, Kei Hiruma and Yusuke Saijo
    • Organizer
      平成30年度日本植物病理学会大会
    • Related Report
      2017 Research-status Report
  • [Presentation] Natural variations of interactions with a root-colonizing endophytic fungus in Arabidopsis thaliana2018

    • Author(s)
      Shion Yamaguchi, Shigetaka Yasuda, Nozomi Kitagawa, Mutusumi Watanabe, Takayuki Tohge、Kei Hiruma, and Yusuke Saijo
    • Organizer
      第59回日本植物生理学会年会
    • Related Report
      2017 Research-status Report
  • [Presentation] Tryptophan metabolite-based control of endophytic fungi in beneficial association with Arabidopsis thaliana2018

    • Author(s)
      Shigetaka Yasuda, Kei Hiruma, Shion Yamaguchi, Lee Tae Hong, Semba Kazuhiko, Mutsumi Watanabe, Takayuki Tohge, Yoshiaki Nakao, Yusuke Saijo
    • Organizer
      第59回日本植物生理学会年会
    • Related Report
      2017 Research-status Report
  • [Presentation] Colletotrichum endophyte-mediated control of fungal pathogenesis in Arabidopsis thaliana2018

    • Author(s)
      Kuldanai Pathompitaknukul, Kei Hiruma and Yusuke Saijo
    • Organizer
      5th Plant Genomics & Gene Editing Congress Asia
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] Phosphate status-dependent control of interactions with pathogenic and endophytic fungi in Arabidopsis thaliana2017

    • Author(s)
      Yusuke Saijo
    • Organizer
      第58回 日本植物生理学会年会
    • Place of Presentation
      鹿児島大学(鹿児島県鹿児島市)
    • Year and Date
      2017-03-16
    • Related Report
      2016 Research-status Report
    • Invited
  • [Presentation] Endophyte-pathogen interactions of closely-related Colletotrichum fungi on the host Arabidopsis thaliana2017

    • Author(s)
      Kuldanai Pathompitaknukul
    • Organizer
      第58回 日本植物生理学会年会
    • Place of Presentation
      鹿児島大学(鹿児島県鹿児島市)
    • Year and Date
      2017-03-16
    • Related Report
      2016 Research-status Report
  • [Presentation] 植物の免疫システムと共棲微生物を介した環境適応2016

    • Author(s)
      西條 雄介
    • Organizer
      第68回 日本生物工学会大会
    • Place of Presentation
      富山国際会議場(富山県富山市)
    • Year and Date
      2016-09-28
    • Related Report
      2016 Research-status Report
    • Invited
  • [Book] Analysis for Protein Glycosylation of Pattern Recognition Receptors in Plants (Plant Pattern Recognition Receptors: Methods and Protocols2017

    • Author(s)
      Inokuchi T, Saijo Y
    • Total Pages
      8
    • Publisher
      Springer
    • Related Report
      2016 Research-status Report

URL: 

Published: 2016-07-20   Modified: 2023-03-08  

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