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The molecular basis of the recognition of nematodes by plants

Research Project

Project/Area Number 16KT0037
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeMulti-year Fund
Section特設分野
Research Field Food Cycle Research
Research InstitutionInstitute of Physical and Chemical Research

Principal Investigator

Kadota Yasuhiro  国立研究開発法人理化学研究所, 環境資源科学研究センター, 専任研究員 (80548975)

Co-Investigator(Kenkyū-buntansha) 植原 健人  国立研究開発法人農業・食品産業技術総合研究機構, 中央農業研究センター, 産学連携コーディネーター (30355458)
Project Period (FY) 2016-07-19 – 2020-03-31
Project Status Completed (Fiscal Year 2019)
Budget Amount *help
¥18,460,000 (Direct Cost: ¥14,200,000、Indirect Cost: ¥4,260,000)
Fiscal Year 2018: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2017: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000)
Fiscal Year 2016: ¥7,540,000 (Direct Cost: ¥5,800,000、Indirect Cost: ¥1,740,000)
Keywords植物寄生線虫 / 植物免疫 / 根圏微生物 / ネコブ線虫 / 作物寄生線虫防除 / ネコブセンチュウ / 線虫抵抗性 / 植物 / 病害抵抗性
Outline of Final Research Achievements

Plant-parasitic nematodes (PPNs) are some of the most devastating pests in agriculture. PPNs can infect almost all cultivated plants, causing about $100 billion in losses annually. Nematode disease is controlled by chemical pesticides, but most of them were banned in EU countries due to their substantial toxicity. To establish an environmentally friendly control method, we focused on rhizosphere microorganisms such as bacteria and free-living nematodes and tried to identify the compounds which confer resistance against PPNs on plants. The biochemical approach identified that peptides from two secreted proteins in free-living nematodes have a strong activity to induce plant immune responses in roots. These peptides might be candidates for potential use in controlling nematode disease in agriculture. For an acculate identification of PPN species, we sequenced the genome of two isolates of root-knot nematode Meloidogyne arenaria that causes substantial loss in Solanaceae plants in Japan.

Academic Significance and Societal Importance of the Research Achievements

線虫の防除には主に強毒性の殺線虫剤が用いられるが、効果は持続的でなく環境負荷も高いことから欧州などでは使用が禁止されている。本研究により、植物に強い免疫反応を誘導するペプチドが自活性線虫から発見された。これらペプチドは線虫防除のための免疫誘導剤として作物に利用できるかもしれない。さらに、これらペプチドは植物に作用して植物に接触した微生物に作用するため、土壌中の多くの微生物には影響を及ぼさないことから環境負荷の低いと考えられる。また、これらペプチドの作用機構は学術的にも大変興味深く、植物によるペプチド認識機構の解明や、ペプチドにより誘導される免疫機構の解明につながる。

Report

(4 results)
  • 2019 Final Research Report ( PDF )
  • 2018 Research-status Report
  • 2017 Research-status Report
  • 2016 Research-status Report
  • Research Products

    (52 results)

All 2019 2018 2017 2016 Other

All Int'l Joint Research (11 results) Journal Article (12 results) (of which Int'l Joint Research: 9 results,  Peer Reviewed: 10 results,  Open Access: 7 results,  Acknowledgement Compliant: 1 results) Presentation (25 results) (of which Invited: 5 results) Remarks (4 results)

  • [Int'l Joint Research] The University of Alabama at Birmingham/University of California, Davis(米国)

    • Related Report
      2018 Research-status Report
  • [Int'l Joint Research] University of Bonn(ドイツ)

    • Related Report
      2018 Research-status Report
  • [Int'l Joint Research] INRA(フランス)

    • Related Report
      2018 Research-status Report
  • [Int'l Joint Research] The Sainsbury laboratory(英国)

    • Related Report
      2018 Research-status Report
  • [Int'l Joint Research] The Sainsbury Laboratory(英国)

    • Related Report
      2017 Research-status Report
  • [Int'l Joint Research] University of California, Davis/The University of Alabama(米国)

    • Related Report
      2017 Research-status Report
  • [Int'l Joint Research] Universidad Politecnica de Madrid(スペイン)

    • Related Report
      2017 Research-status Report
  • [Int'l Joint Research] University of Bonn,(ドイツ)

    • Related Report
      2017 Research-status Report
  • [Int'l Joint Research] University of Toronto(カナダ)

    • Related Report
      2017 Research-status Report
  • [Int'l Joint Research]

    • Related Report
      2017 Research-status Report
  • [Int'l Joint Research] The University of Bonn(ドイツ)

    • Related Report
      2016 Research-status Report
  • [Journal Article] Chitin perception in plasmodesmata identifies subcellular, context-specific immune signalling in plants2019

    • Author(s)
      Cheval Cecilia、Johnston Matthew、Samwald Sebastian、Liu Xiaokun、Bellandi Annalisa、Breakspear Andrew、Kadota Yasuhiro、Zipfel Cyril、Faulkner Christine
    • Journal Title

      bioRxiv

      Volume: -

    • DOI

      10.1101/611582

    • Related Report
      2018 Research-status Report
    • Int'l Joint Research
  • [Journal Article] Quantitative phosphoproteomic analysis reveals common regulatory mechanisms between effector‐ and PAMP‐triggered immunity in plants2018

    • Author(s)
      Kadota Yasuhiro、Liebrand Thomas W. H.、Goto Yukihisa、Sklenar Jan、Derbyshire Paul、Menke Frank L. H.、Torres Miguel‐Angel、Molina Antonio、Zipfel Cyril、Coaker Gitta、Shirasu Ken
    • Journal Title

      New Phytologist

      Volume: 221 Issue: 4 Pages: 2160-2175

    • DOI

      10.1111/nph.15523

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Phosphocode-dependent functional dichotomy of a common co-receptor in plant signalling2018

    • Author(s)
      Perraki Artemis、DeFalco Thomas A.、Derbyshire Paul、Avila Julian、S?r? David、Sklenar Jan、Qi Xingyun、Stransfeld Lena、Schwessinger Benjamin、Kadota Yasuhiro、Macho Alberto P.、Jiang Shushu、Couto Daniel、Torii Keiko U.、Menke Frank L. H.、Zipfel Cyril
    • Journal Title

      Nature

      Volume: 561 Issue: 7722 Pages: 248-252

    • DOI

      10.1038/s41586-018-0471-x

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] High-Quality Genome Sequence of the Root-Knot Nematode Meloidogyne arenaria Genotype A2-O2018

    • Author(s)
      Kazuki Sato, Yasuhiro Kadota, Pamela Gan, Takahiro Bino, Taketo Uehara, Katsushi Yamaguchi, Yasunori Ichihashi, Noriko Maki, Hideaki Iwahori, Takamasa Suzuki, Shuji Shigenobu, Ken Shirasu
    • Journal Title

      Genome Announcements

      Volume: 6 Issue: 26 Pages: 00519-18

    • DOI

      10.1128/genomea.00519-18

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Same tune, different song-cytokinins as virulence factors in plant-pathogen interactions?2018

    • Author(s)
      Thomas Spallek, Pamela Gan, Yasuhiro Kadota and Ken Shirasu,
    • Journal Title

      Current Opinion in Plant Biology

      Volume: 44 Pages: 82-87

    • DOI

      10.1016/j.pbi.2018.03.002

    • Related Report
      2018 Research-status Report 2017 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Molecular plant-root knot nematodes interactions2018

    • Author(s)
      Yasuhiro Kadota, Kazuki Sato, Pamela Gan, Taketo Uehara, Takahiro Bino, Katsushi Yamaguchi, Yasunori Ichihashi, Hideaki Iwahori, Noriko Maki, Takamasa Suzuki, Shuji Shigenobu, Ken Shirasu
    • Journal Title

      PSJ Plant-Microbe Interactions Symposium Report

      Volume: 53 Pages: 87-96

    • Related Report
      2018 Research-status Report
  • [Journal Article] Differences in parasitism of Meloidogyne incognita and two genotypes of M. arenaria on Solanum torvum in Japan,2017

    • Author(s)
      Uehara T, Tateishi Y, Kadota Y, Iwahori H,
    • Journal Title

      Journal of Phytopathology

      Volume: 165 Issue: 9 Pages: 575-579

    • DOI

      10.1111/jph.12594

    • Related Report
      2017 Research-status Report
    • Peer Reviewed
  • [Journal Article] Differences in parasitism of Meloidogyne incognita and two genotypes of M. 5 arenaria on Solanum torvum in Japan2017

    • Author(s)
      Uehara T, Tateishi Y, Kadota Y, Iwahori H
    • Journal Title

      Journal of Phytopathology

      Volume: 印刷中

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Immunoprecipitation of plasma membrane receptor-like kinases for identification of phosphorylation sites and associated proteins.2016

    • Author(s)
      Kadota Y, Macho AP, Zipfel C
    • Journal Title

      Methods in Molecular Biology, Springer

      Volume: 1363 Pages: 133-144

    • DOI

      10.1007/978-1-4939-3115-6_11

    • ISBN
      9781493931149, 9781493931156
    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] The Arabidopsis Malectin-Like/LRR-RLK IOS1 is Critical for BAK1-Dependent and BAK1-Independent Pattern-Triggered Immunity.2016

    • Author(s)
      Yeh YH, Panzeria D*, Kadota Y*, Huanga YC, Huanga PY, Taoa CN, Roux M, Chiena HC, China TC, Chua PW, Zipfel C, Zimmerlia L (*co-2nd author)
    • Journal Title

      The Plant Cell

      Volume: 28 Pages: 1701-1721

    • DOI

      10.1105/tpc.16.00313

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] NbCSPR underlies age-dependent immune responses to bacterial cold shock protein in Nicotiana benthamiana.2016

    • Author(s)
      Saur IM, Kadota Y, Sklenar J, Holton NJ, Smakowska E, Belkhadir Y, Zipfel C, Rathjen JP
    • Journal Title

      Proc Natl Acad Sci U S A

      Volume: 113 Issue: 12 Pages: 3389-3394

    • DOI

      10.1073/pnas.1511847113

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] The Arabidopsis NADPH oxidases RbohD and RbohF display differential expression patterns and contributions during plant immunity.2016

    • Author(s)
      Morales J, Kadota Y, Zipfel C, Molina A, Torres MA
    • Journal Title

      Journal of Experimental Botany

      Volume: 67 Issue: 6 Pages: 1663-1676

    • DOI

      10.1093/jxb/erv558

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Presentation] アレナリアネコブセンチュウによる線虫抵抗性植物Solanum torvumの免疫応答抑制2019

    • Author(s)
      佐藤 一輝, 門田 康弘, Gan Pamela, 植原 健人, 尾納 隆大, 山口 勝司, 市橋 泰範, 岩堀 英晶, 槇 紀子, 重信 秀治, 鈴木 孝征, 白須 賢
    • Organizer
      平成31年度 日本植物病理学会大会
    • Related Report
      2018 Research-status Report
  • [Presentation] The suppression of immune responses in nematode-resistant plant Solanum torvum by root-knot nematode, Meloidogyne arenaria2019

    • Author(s)
      Kazuki Sato、Yasuhiro Kadota、Pamela Gan、Taketo Uehara、Takahiro Bino、Katsushi Yamaguchi、Yasunori Ichihashi、Hideaki Iwahori、Noriko Maki、Shuji Shigenobu、Takamasa Suzuki、Ken Shirasu
    • Organizer
      第60回 日本植物生理学会年会
    • Related Report
      2018 Research-status Report
  • [Presentation] 新規PRR複合体構成因子REAL1によるPAMP誘導性免疫の制御機構2019

    • Author(s)
      後藤幸久, 門田康弘, 松井英譲, Jan Sklenar, Paul Derbyshire , Frank Menke, 中神弘史, Cyril Zipfel ,白須賢
    • Organizer
      平成31年度 日本植物病理学会大会
    • Related Report
      2018 Research-status Report
  • [Presentation] Regulatory mechanism of PAMP-triggered immunity by REAL1, a novel component of PRR complex2019

    • Author(s)
      Yukihisa Goto, Yasuhiro Kadota, Hidenori Matsui, Jan Sklenar, Paul Derbyshire, Frank Menke, Hirofumi Nakagami, Cyril Zipfel, Ken Shirasu
    • Organizer
      第60回 日本植物生理学会年会
    • Related Report
      2018 Research-status Report
  • [Presentation] ネコブセンチュウの病原性と植物免疫の解明2018

    • Author(s)
      門田 康弘,佐藤 一輝, Pamela Gan, 植原 健人, 尾納 隆大, 山口 勝司, 市橋 泰範, 岩堀 英晶, 槇 紀子, 鈴木 孝征, 重信 秀治, 白須 賢
    • Organizer
      2018年 線虫学会 シンポジウム“「新」線虫研究 加速する多様化と応用への期待”
    • Related Report
      2018 Research-status Report
    • Invited
  • [Presentation] 植物と線虫の戦いを分子レベルで紐解く2018

    • Author(s)
      門田 康弘,佐藤 一輝・植原 健人・Pamela Gan・尾納 隆大・山口 勝司・市橋 泰範・岩堀 英晶・槇 紀子・鈴木 孝征・重信 秀治・白須 賢
    • Organizer
      (第 53 回) 感染生理談話会
    • Related Report
      2018 Research-status Report
    • Invited
  • [Presentation] 受容体キナーゼREAL1はPAMP受容体のタンパク質量を調節することで免疫応答を負に制御する2018

    • Author(s)
      後藤幸久, 門田康弘, 松井英譲, Jan Sklenar, Paul Derbyshire , Frank Menke, 中神弘史, Cyril Zipfel ,白須賢
    • Organizer
      新学術領域研究植物の成長可塑性を支える環境認識と記憶の自律分散型統御システム第4回 若手の会
    • Related Report
      2018 Research-status Report
  • [Presentation] Dual RNA-sequencing of root-knot nematodes and their host plants reveals plant immune responses and nematode virulent effectors.2018

    • Author(s)
      Goto, Y, Kadota, Y, Matsui, H, Sklenar, J, Derbyshire, P, Menke, F, Nakagami, H, Zipfel, C, Shirasu, K.
    • Organizer
      第59回日本植物生理学会年会
    • Related Report
      2017 Research-status Report
  • [Presentation] Dual RNA-sequencing of root-knot nematodes and their host plants reveals plant immune responses and nematode virulent effectors.2018

    • Author(s)
      Sato, K, Kadota, Y, Ichihashi, Y, Gan, P, Uehara, T, Iwahori, H, Maki, N, Suzuki, T, Shirasu, K.
    • Organizer
      第59回日本植物生理学会年会
    • Related Report
      2017 Research-status Report
  • [Presentation] PAMP情報伝達系を負に制御する受容体型キナーゼREAL1の機能解析2018

    • Author(s)
      後藤幸久、 門田康弘、松井英譲,、Jan Sklenar、Paul Derbyshire、Frank Menke、中神弘史、Cyril Zipfel、白須賢.
    • Organizer
      平成30年度日本植物病理学会大会
    • Related Report
      2017 Research-status Report
  • [Presentation] ネコブセンチュウの感染に対する植物の免疫応答と線虫の病原性因子のDual RNA-seq解析2018

    • Author(s)
      佐藤一輝、門田康弘、Pamela Gan、植原健人、尾納隆大、山口勝司、市橋泰範、岩堀英晶、槇紀子、重信秀治、鈴木孝征、白須賢
    • Organizer
      平成30年度日本植物病理学会大会
    • Related Report
      2017 Research-status Report
  • [Presentation] REAL1, a novel factor of PRR complex negatively regulates PAMP-triggered signal transduction pathways2018

    • Author(s)
      Goto Y, Kadota Y, Matsui H, Sklenar J, Derbyshire P, Frank Menke, Nakagami H, Zipfel C, Shirasu K,
    • Organizer
      Keystone symposia Plant Signaling: Molecular Pathways and Network Integration,
    • Related Report
      2017 Research-status Report
  • [Presentation] PRR複合体の新規因子REAL1はPAMP情報伝達系を負に制御する2017

    • Author(s)
      後藤 幸久, 門田 康弘, 松井 英譲, Jan Sklenar, Paul Derbyshire, Frank Menke, 中神 弘史, Cyril Zipfel, 白須 賢
    • Organizer
      平成29年度日本植物病理学会大会
    • Place of Presentation
      マリオス、アイーナ・岩手県民情報交流センター(岩手県盛岡市)
    • Year and Date
      2017-04-27
    • Related Report
      2016 Research-status Report
  • [Presentation] ネコブセンチュウの病原性機構と植物の感染防御機構の解明2017

    • Author(s)
      門田 康弘, 市橋 泰範, 植原 健人, 岩堀 英晶, 槇 紀子, 鈴木 孝征, 白須 賢
    • Organizer
      平成29年度日本植物病理学会大会
    • Place of Presentation
      マリオス、アイーナ・岩手県民情報交流センター(岩手県盛岡市)
    • Year and Date
      2017-04-26
    • Related Report
      2016 Research-status Report
  • [Presentation] Plant immunity against Root-knot nematode2017

    • Author(s)
      Kadota Y, Ichihashi Y, Uehara T, Iwahori H, Maki N, Suzuki T, Shirasu K
    • Organizer
      第58回日本植物生理学会
    • Place of Presentation
      鹿児島大学群元キャンパス(鹿児島県鹿児島市)
    • Year and Date
      2017-03-17
    • Related Report
      2016 Research-status Report
  • [Presentation] Phosphorylation-dependent self-ubiquitination of ATL31 during plant immunity2017

    • Author(s)
      Yasuda S, Hasegawa Y, Kadota Y, Sato T, Saijo Y, Yamaguchi J
    • Organizer
      第58回日本植物生理学会
    • Place of Presentation
      鹿児島大学群元キャンパス(鹿児島県鹿児島市)
    • Year and Date
      2017-03-16
    • Related Report
      2016 Research-status Report
  • [Presentation] Identification of novel regulators of the NADPH oxidase RBOHD during plant immunity2017

    • Author(s)
      後藤 幸久, 門田 康弘, 松井 英譲, Jan Sklenar, Paul Derbyshire, Frank Menke, 中神 弘史, Cyril Zipfel, 白須 賢
    • Organizer
      第58回日本植物生理学会
    • Place of Presentation
      鹿児島大学群元キャンパス(鹿児島県鹿児島市)
    • Year and Date
      2017-03-16
    • Related Report
      2016 Research-status Report
  • [Presentation] 植物と病原微生物の戦いを分子レベルで紐解く2017

    • Author(s)
      門田康弘
    • Organizer
      岡山植物病理セミナー
    • Related Report
      2017 Research-status Report
    • Invited
  • [Presentation] Regulation of NADPH oxidase, RBOHD, during plant immunity2017

    • Author(s)
      Yasuhiro Kadota
    • Organizer
      University of Bonn シンポジウム “Role of ROS in Plant Stress Signaling”,
    • Related Report
      2017 Research-status Report
    • Invited
  • [Presentation] ネコブセンチュウの病原性機構と植物の免疫機構の解明2017

    • Author(s)
      門田康弘, 佐藤一輝, 市橋泰範, 植原健人, 岩堀英晶, 槇 紀子, 鈴木孝征, 白須賢
    • Organizer
      平成29年度日本線虫学会大会
    • Related Report
      2017 Research-status Report
  • [Presentation] ネコブセンチュウの病原性機構と植物の感染防御機構の解明2017

    • Author(s)
      門田康弘, 市橋泰範, 植原健人, 岩堀英晶, 槇 紀子, 鈴木孝征, 白須賢
    • Organizer
      平成29年度日本植物病理学会大会
    • Related Report
      2017 Research-status Report
  • [Presentation] Plant immunity against Meloidogyne arenaria2017

    • Author(s)
      Yasuhiro Kadota
    • Organizer
      International workshop on Plant Parasitic Nematode in Kumamoto
    • Related Report
      2017 Research-status Report
  • [Presentation] 植物寄生線虫の病原性機構と植物の抵抗性機構2016

    • Author(s)
      門田 康弘, 白須 賢
    • Organizer
      日本植物学会第80回大会 シンポジウム「Induced Development:環境要因に誘発される発生の多様性と共通性」
    • Place of Presentation
      沖縄コンベンションセンター(沖縄県宜野湾市)
    • Year and Date
      2016-09-16
    • Related Report
      2016 Research-status Report
    • Invited
  • [Presentation] ネコブセンチュウの病原性機構と植物の感染防御機構の解明2016

    • Author(s)
      門田 康弘, 市橋 泰範, 植原 健人, 岩堀 英晶, 槇 紀子, 鈴木 孝征, 白須 賢
    • Organizer
      日本線虫学会第24回大会
    • Place of Presentation
      東京農工大学小金井キャンパス(東京都小金井市)
    • Year and Date
      2016-09-14
    • Related Report
      2016 Research-status Report
  • [Presentation] 感染防御応答に重要なNADPH oxidase RBOHDの制御機構解明2016

    • Author(s)
      後藤 幸久, 門田 康弘, 松井 英譲, Jan Sklenar, Paul Derbyshire, Frank Menke, 中神 弘史, Cyril Zipfel, 白須 賢
    • Organizer
      感染生理談話会
    • Place of Presentation
      神戸市須磨浦海岸 シーパル須磨(兵庫県神戸市)
    • Year and Date
      2016-08-11
    • Related Report
      2016 Research-status Report
  • [Remarks] RIKEN CSRS Plant Immunity Research Group

    • URL

      http://plantimmunity.riken.jp/

    • Related Report
      2018 Research-status Report
  • [Remarks] 理化学研究所 環境資源科学研究センター 植物免疫研究グループ

    • Related Report
      2017 Research-status Report
  • [Remarks] http://plantimmunity.riken.jp/

    • Related Report
      2017 Research-status Report
  • [Remarks] 理化学研究所 植物免疫研究グループ ホームページ

    • URL

      http://plantimmunity.riken.jp/

    • Related Report
      2016 Research-status Report

URL: 

Published: 2016-07-20   Modified: 2023-03-08  

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