The molecular basis of the recognition of nematodes by plants
Project/Area Number |
16KT0037
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Research Category |
Grant-in-Aid for Scientific Research (B)
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Allocation Type | Multi-year Fund |
Section | 特設分野 |
Research Field |
Food Cycle Research
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Research Institution | Institute of Physical and Chemical Research |
Principal Investigator |
Kadota Yasuhiro 国立研究開発法人理化学研究所, 環境資源科学研究センター, 専任研究員 (80548975)
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Co-Investigator(Kenkyū-buntansha) |
植原 健人 国立研究開発法人農業・食品産業技術総合研究機構, 中央農業研究センター, 産学連携コーディネーター (30355458)
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Project Period (FY) |
2016-07-19 – 2020-03-31
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Project Status |
Completed (Fiscal Year 2019)
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Budget Amount *help |
¥18,460,000 (Direct Cost: ¥14,200,000、Indirect Cost: ¥4,260,000)
Fiscal Year 2018: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2017: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000)
Fiscal Year 2016: ¥7,540,000 (Direct Cost: ¥5,800,000、Indirect Cost: ¥1,740,000)
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Keywords | 植物寄生線虫 / 植物免疫 / 根圏微生物 / ネコブ線虫 / 作物寄生線虫防除 / ネコブセンチュウ / 線虫抵抗性 / 植物 / 病害抵抗性 |
Outline of Final Research Achievements |
Plant-parasitic nematodes (PPNs) are some of the most devastating pests in agriculture. PPNs can infect almost all cultivated plants, causing about $100 billion in losses annually. Nematode disease is controlled by chemical pesticides, but most of them were banned in EU countries due to their substantial toxicity. To establish an environmentally friendly control method, we focused on rhizosphere microorganisms such as bacteria and free-living nematodes and tried to identify the compounds which confer resistance against PPNs on plants. The biochemical approach identified that peptides from two secreted proteins in free-living nematodes have a strong activity to induce plant immune responses in roots. These peptides might be candidates for potential use in controlling nematode disease in agriculture. For an acculate identification of PPN species, we sequenced the genome of two isolates of root-knot nematode Meloidogyne arenaria that causes substantial loss in Solanaceae plants in Japan.
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Academic Significance and Societal Importance of the Research Achievements |
線虫の防除には主に強毒性の殺線虫剤が用いられるが、効果は持続的でなく環境負荷も高いことから欧州などでは使用が禁止されている。本研究により、植物に強い免疫反応を誘導するペプチドが自活性線虫から発見された。これらペプチドは線虫防除のための免疫誘導剤として作物に利用できるかもしれない。さらに、これらペプチドは植物に作用して植物に接触した微生物に作用するため、土壌中の多くの微生物には影響を及ぼさないことから環境負荷の低いと考えられる。また、これらペプチドの作用機構は学術的にも大変興味深く、植物によるペプチド認識機構の解明や、ペプチドにより誘導される免疫機構の解明につながる。
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Report
(4 results)
Research Products
(52 results)
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[Journal Article] High-Quality Genome Sequence of the Root-Knot Nematode Meloidogyne arenaria Genotype A2-O2018
Author(s)
Kazuki Sato, Yasuhiro Kadota, Pamela Gan, Takahiro Bino, Taketo Uehara, Katsushi Yamaguchi, Yasunori Ichihashi, Noriko Maki, Hideaki Iwahori, Takamasa Suzuki, Shuji Shigenobu, Ken Shirasu
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Journal Title
Genome Announcements
Volume: 6
Issue: 26
Pages: 00519-18
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Molecular plant-root knot nematodes interactions2018
Author(s)
Yasuhiro Kadota, Kazuki Sato, Pamela Gan, Taketo Uehara, Takahiro Bino, Katsushi Yamaguchi, Yasunori Ichihashi, Hideaki Iwahori, Noriko Maki, Takamasa Suzuki, Shuji Shigenobu, Ken Shirasu
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Journal Title
PSJ Plant-Microbe Interactions Symposium Report
Volume: 53
Pages: 87-96
Related Report
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[Journal Article] The Arabidopsis Malectin-Like/LRR-RLK IOS1 is Critical for BAK1-Dependent and BAK1-Independent Pattern-Triggered Immunity.2016
Author(s)
Yeh YH, Panzeria D*, Kadota Y*, Huanga YC, Huanga PY, Taoa CN, Roux M, Chiena HC, China TC, Chua PW, Zipfel C, Zimmerlia L (*co-2nd author)
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Journal Title
The Plant Cell
Volume: 28
Pages: 1701-1721
DOI
Related Report
Peer Reviewed / Int'l Joint Research
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[Presentation] アレナリアネコブセンチュウによる線虫抵抗性植物Solanum torvumの免疫応答抑制2019
Author(s)
佐藤 一輝, 門田 康弘, Gan Pamela, 植原 健人, 尾納 隆大, 山口 勝司, 市橋 泰範, 岩堀 英晶, 槇 紀子, 重信 秀治, 鈴木 孝征, 白須 賢
Organizer
平成31年度 日本植物病理学会大会
Related Report
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[Presentation] ネコブセンチュウの病原性と植物免疫の解明2018
Author(s)
門田 康弘,佐藤 一輝, Pamela Gan, 植原 健人, 尾納 隆大, 山口 勝司, 市橋 泰範, 岩堀 英晶, 槇 紀子, 鈴木 孝征, 重信 秀治, 白須 賢
Organizer
2018年 線虫学会 シンポジウム“「新」線虫研究 加速する多様化と応用への期待”
Related Report
Invited
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[Presentation] Dual RNA-sequencing of root-knot nematodes and their host plants reveals plant immune responses and nematode virulent effectors.2018
Author(s)
Goto, Y, Kadota, Y, Matsui, H, Sklenar, J, Derbyshire, P, Menke, F, Nakagami, H, Zipfel, C, Shirasu, K.
Organizer
第59回日本植物生理学会年会
Related Report
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[Presentation] Dual RNA-sequencing of root-knot nematodes and their host plants reveals plant immune responses and nematode virulent effectors.2018
Author(s)
Sato, K, Kadota, Y, Ichihashi, Y, Gan, P, Uehara, T, Iwahori, H, Maki, N, Suzuki, T, Shirasu, K.
Organizer
第59回日本植物生理学会年会
Related Report
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[Presentation] REAL1, a novel factor of PRR complex negatively regulates PAMP-triggered signal transduction pathways2018
Author(s)
Goto Y, Kadota Y, Matsui H, Sklenar J, Derbyshire P, Frank Menke, Nakagami H, Zipfel C, Shirasu K,
Organizer
Keystone symposia Plant Signaling: Molecular Pathways and Network Integration,
Related Report
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[Presentation] PRR複合体の新規因子REAL1はPAMP情報伝達系を負に制御する2017
Author(s)
後藤 幸久, 門田 康弘, 松井 英譲, Jan Sklenar, Paul Derbyshire, Frank Menke, 中神 弘史, Cyril Zipfel, 白須 賢
Organizer
平成29年度日本植物病理学会大会
Place of Presentation
マリオス、アイーナ・岩手県民情報交流センター(岩手県盛岡市)
Year and Date
2017-04-27
Related Report
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