Elucidation of Molecular Mechanism Responsible for Peroxidase Activity of a Heme-Nucleic Acid Complex through the Nature of an Axial H2O ligand and Its Application to Design of Novel Catalysts

• Project/Area Number: 16KT0048
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Multi-year Fund
• Section: 特設分野
• Research Field: Transition State Control
• Research Institution: University of Tsukuba
• Principal Investigator: Yamamoto Yasuhiko 筑波大学, 数理物質系, 教授 (00191453)
• Project Period (FY): 2016-07-19 – 2020-03-31
• Project Status: Completed (Fiscal Year 2019)
• Budget Amount: ¥18,460,000 (Direct Cost: ¥14,200,000、Indirect Cost: ¥4,260,000); Fiscal Year 2019: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000); Fiscal Year 2018: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000); Fiscal Year 2017: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2016: ¥7,540,000 (Direct Cost: ¥5,800,000、Indirect Cost: ¥1,740,000)
• Keywords: 四重鎖DNA / ヘム / G-カルテット / 酸化触媒作用 / テロメア / 機能性核酸 / 触媒機構 / 軸配位子 / 水分子

Outline of Final Research Achievements

The molecular mechanism of the peroxidase activity of a complex composed of a G-quadruplex DNA formed from guanine-rich DNA and heme, found as prosthetic groups of various hemoproteins abundant in nature, has been elucidated. A water molecule (H2O) is located at the interface between the heme and DNA in the complex and is coordinated to the heme Fe atom. We found that the axial H2O in the complex is capable of being an electron-donating ligand which increases the Lewis basicity of the heme Fe atom to facilitate heterolytic O-O bond cleavage of Fe-bound peroxide (H2O2) to promote the formation of a high-valent iron(IV)oxo porphyrin π-cation radical.

Academic Significance and Societal Importance of the Research Achievements

ヘム核酸複合体の酸化触媒作用の遷移状態が軸配位子H2Oの電子的性質により調節される機構の解明を通して、H2Oの新しい機能が発見された。当該H2Oは、電子的性質が特異であるだけでなく、ヘム核酸複合体のヘムと四重鎖DNAの接触界面で孤立しているため、その運動性も特異である。これらの知見は、H2Oを利用する新しい反応の発見に役立つことが期待される。また、ヘムが核酸の補欠分子族として機能することから、ヘムタンパク質の機能を変えずにタンパク質部分を核酸で置換することが可能であることが示された。

Research Products

• [Int'l Joint Research] Simon Fraser University(カナダ)
• [Int'l Joint Research] Simon Fraser Univsrsity(Canada)
• [Journal Article] Characterization of Structure and Catalytic Activity of a Complex between Heme and an All Parallel-Stranded Tetrameric G-Quadruplex Formed from DNA/RNA Chimera Sequence d(TTA)r(GGG)dT (2020)
  • Author(s): Hayasaka Kosuke、Shibata Tomokazu、Sugahara Aya、Momotake Atsuya、Matsui Toru、Neya Saburo、Ishizuka Takumi、Xu Yan、Yamamoto Yasuhiko
  • Journal Title: Bulletin of the Chemical Society of Japan Volume: 93 Issue: 5 Pages: 621-629
  • DOI: 10.1246/bcsj.20200013
  • NAID: 130007842975
  • Peer Reviewed
• [Journal Article] Specific Binding of an Anionic Phthalocyanine Derivative to G-Quadruplex DNAs (2020)
  • Author(s): Uchiyama Mami、Momotake Atsuya、Kobayashi Nagao、Yamamoto Yasuhiko
  • Journal Title: Chemistry Letters Volume: 49 Issue: 5 Pages: 530-533
  • DOI: 10.1246/cl.200110
  • NAID: 130007840180
  • Peer Reviewed
• [Journal Article] Multimodal Multiphoton Imaging of the Lipid Bilayer by Dye-Based Sum-Frequency Generation and Coherent Anti-Stokes Raman Scattering (2020)
  • Author(s): Mizuguchi Takaha、Momotake Atsuya、Hishida Mafumi、Yasui Masato、Yamamoto Yasuhiko、Saiki Toshiharu、Nuriya Mutsuo
  • Journal Title: Analytical Chemistry Volume: 92 Issue: 8 Pages: 5656-5660
  • DOI: 10.1021/acs.analchem.0c00673
  • Peer Reviewed
• [Journal Article] Monitoring the morphological evolution of giant vesicles by azo dye-based sum-frequency generation (SFG) microscopy (2020)
  • Author(s): Momotake Atsuya、Mizuguchi Takaha、Hishida Mafumi、Yamamoto Yasuhiko、Yasui Masato、Nuriya Mutsuo
  • Journal Title: Colloids and Surfaces B: Biointerfaces Volume: 186 Pages: 110716-110716
  • DOI: 10.1016/j.colsurfb.2019.110716
  • Peer Reviewed / Open Access
• [Journal Article] Identification of Intermediates in Peroxidase Catalytic Cycle of a DNAzyme Possessing Heme (2019)
  • Author(s): Shinomiya Ryosuke、Araki Haruka、Momotake Atsuya、Kotani Hiroaki、Kojima Takahiko、Yamamoto Yasuhiko
  • Journal Title: Bulletin of the Chemical Society of Japan Volume: 92 Issue: 10 Pages: 1729-1736
  • DOI: 10.1246/bcsj.20190157
  • NAID: 130007728639
  • Peer Reviewed
• [Journal Article] Structures and Catalytic Activities of Complexes between Heme and All Parallel-Stranded Monomeric G-Quadruplex DNAs (2018)
  • Author(s): Yamamoto Yasuhiko、Araki Haruka、Shinomiya Ryosuke、Hayasaka Kosuke、Nakayama Yusaku、Ochi Kentaro、Shibata Tomokazu、Momotake Atsuya、Ohyama Takako、Hagihara Masaki、Hemmi Hikaru
  • Journal Title: Biochemistry Volume: 57 Issue: 41 Pages: 5938-5948
  • DOI: 10.1021/acs.biochem.8b00792
  • Peer Reviewed
• [Journal Article] Characterization of Catalytic Activities and Heme Coordination Structures of Heme?DNA Complexes Composed of Some Chemically Modified Hemes and an All Parallel-Stranded Tetrameric G-Quadruplex DNA Formed from d(TTAGGG) (2018)
  • Author(s): Shinomiya Ryosuke、Katahira Yuya、Araki Haruka、Shibata Tomokazu、Momotake Atsuya、Yanagisawa Sachiko、Ogura Takashi、Suzuki Akihiro、Neya Saburo、Yamamoto Yasuhiko
  • Journal Title: Biochemistry Volume: 57 Issue: 41 Pages: 5930-5937
  • DOI: 10.1021/acs.biochem.8b00793
  • Peer Reviewed
• [Journal Article] An Intramolecular Antiparallel G-quadruplex Formed by Human Telomere RNA (2018)
  • Author(s): Chao-Da Xiao, Tomokazu Shibata, Yasuhiko Yamamoto, and Yan Xu
  • Journal Title: Chemical Communications Volume: 54 Issue: 32 Pages: 3944-3946
  • DOI: 10.1039/c8cc01427b
  • Peer Reviewed
• [Journal Article] Synergistic Effect of Distal Polar Interactions in Myoglobin and Their Structural Consequences (2018)
  • Author(s): Watanabe Miho、Kanai Yuki、Nakamura Shunpei、Nishimura Ryu、Shibata Tomokazu、Momotake Atsuya、Yanagisawa Sachiko、Ogura Takashi、Matsuo Takashi、Hirota Shun、Neya Saburo、Suzuki Akihiro、Yamamoto Yasuhiko
  • Journal Title: Inorganic Chemistry Volume: 57 Issue: 22 Pages: 14269-14279
  • DOI: 10.1021/acs.inorgchem.8b02302
  • Peer Reviewed
• [Journal Article] A Nuclear Resonance Vibrational Spectroscopic Study of Oxy Myoglobins Reconstituted with Chemically Modified Heme Cofactors: Insights into the Fe?O2 Bonding and Internal Dynamics of the Protein (2018)
  • Author(s): Ohta Takehiro、Shibata Tomokazu、Kobayashi Yasuhiro、Yoda Yoshitaka、Ogura Takashi、Neya Saburo、Suzuki Akihiro、Seto Makoto、Yamamoto Yasuhiko
  • Journal Title: Biochemistry Volume: 57 Issue: 48 Pages: 6649-6652
  • DOI: 10.1021/acs.biochem.8b00829
  • Peer Reviewed
• [Journal Article] NMR of Paramagnetic Compounds (2018)
  • Author(s): Yasuhiko Yamamoto and Tomokazu Shibata
  • Journal Title: Experimental Approaches of NMR Spectroscopy, Methodology and Application to Life Science and Materials Science Pages: 491-518
  • Peer Reviewed
• [Journal Article] Characterization of Heme Orientational Disorder in a Myoglobin Reconstituted with a Trifluoromethyl-Group-Substituted Heme Cofactor (2017)
  • Author(s): 1.Yuki Kanai, Ayaka Harada, Tomokazu Shibata, Ryu Nishimura, Kosuke Namiki, Miho Watanabe, Shunpei Nakamura, Fumiaki Yuymoto, Toshiya Senda, Akihiro Suzuki, Saburo Neya, and Yasuhiko Yamamoto
  • Journal Title: Biochemistry Volume: ５６ Issue: 34 Pages: 4500-4508
  • DOI: 10.1021/acs.biochem.7b00457
  • Peer Reviewed
• [Journal Article] Effects of Heme Electronic Structure and Distal Polar Interaction on Functional and Vibrational Properties of Myoglobin (2016)
  • Author(s): Y. Kanai, R. Nishimura, K. Nishiyama, T. Shibata, S. Yanagisawa, T. Ogura, T. Matsuo, S. Hirota, S. Neya, A. Suzuki, Y. Yamamoto
  • Journal Title: Inorg. Chem. Volume: 55 Issue: 4 Pages: 1613-1622
  • DOI: 10.1021/acs.inorgchem.5b02520
  • Peer Reviewed / Open Access
• [Journal Article] Ferrous Heme Iron in Deoxy Myoglobin Reconstitutedwith Trifluoromethyl Group-Substituted Heme Cofactors (2016)
  • Author(s): T. Shibata, Y. Kanai, R. Nishimura, L. Xu, Y. Moritaka, A. Suzuki, S. Neya, M. Nakamura, and Y. Yamamoto
  • Journal Title: Inorganic Chemistry Volume: 55 Issue: 23 Pages: 12128-12136
  • DOI: 10.1021/acs.inorgchem.6b01360
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Characterization of the interaction between heme and a parallel G-quadruplexDNA formed from d(TTGAGG) (2016)
  • Author(s): T. Shibata, Y. Nakayama, Y. Katahira, H. Tai, Y.Moritaka, Y. Nakano, and Y. Yamamoto
  • Journal Title: Biochim. Biophys. Acta Volume: - Issue: 5 Pages: 1264-1270
  • DOI: 10.1016/j.bbagen.2016.11.005
  • Peer Reviewed / Acknowledgement Compliant
• [Presentation] メソ位にトリフルオロメチル基を導入したヘムをもつミオグロビンの研究 (2020)
  • Author(s): 村田莉恵、百武篤也、山本泰彦、鈴木秋弘、松尾貴史、廣田俊、根矢三郎
  • Organizer: 日本化学会第100春季年会 (2020)
• [Presentation] 四重鎖DNAとフタロシアニン誘導体の複合体 (2020)
  • Author(s): 内山真見、柴田友和、百武篤也、池上崇久、松井亨、山本泰彦
  • Organizer: 日本化学会第100春季年会 (2020)
• [Presentation] カチオン性共重合体によるヘム-DNA 複合体のペルオキシダーゼ活性の増大 (2020)
  • Author(s): 荒木はるか、百武篤也、山本泰彦、落合拓郎、嶋田直彦、丸山厚
  • Organizer: 日本化学会第100春季年会 (2020)
• [Presentation] ヒトテロメア関連塩基配列のDNA が2 分子で形成する平行型四重鎖DNA およびそのヘムとの複合体の研究 (2020)
  • Author(s): 岡本千奈、篠宮僚介、山田果央、百武篤也、山本泰彦
  • Organizer: 日本化学会第100春季年会 (2020)
• [Presentation] ヘム-DNA 複合体のペルオキシダーゼ活性におけるPush 効果とPull 効果の相関関係の解析 (2020)
  • Author(s): 萩原翔太、篠宮僚介、百武篤也、鈴木秋弘、根矢三郎、山本泰彦
  • Organizer: 日本化学会第100春季年会 (2020)
• [Presentation] ヘム－DNA 複合体のイミダゾール付加物の解析 (2020)
  • Author(s): 中島優輔、百武篤也、山本泰彦
  • Organizer: 日本化学会第100春季年会 (2020)
• [Presentation] Deoxyribozyme Possessing Heme (2019)
  • Author(s): Y. Yamamoto, H. Araki, K. Hayasaka, S. Nakamura,T. Shibata, A. Momotake, and H. Hemmi
  • Organizer: 61st Experimental Nuclear Magnetic Resonance Conference
  • Int'l Joint Research
• [Presentation] Structures and Catalytic Activities of DNA Complexes Consisting of Chemically Modified Heme and Tetrameric G-Quadruplex DNA (2019)
  • Author(s): R. Shinomiya, A. Momotake, Y. Katahira, H. Araki, T. Shibata, S. Yanagisawa, T. Ogura, A. Suzuki, S. Neya, and Y. Yamamoto
  • Organizer: 15th International Symposium on Applied Bioinorganic Chemistry (ISABC15)
  • Int'l Joint Research
• [Presentation] Heme-DNAzyme (2019)
  • Author(s): Y. Yamamoto, R. Shinomiya, H. Araki, T. Shibata, A. Momotake, S. Yanagisawa, T. Ogura, A. Suzuki, S. Neya, and H. Hemmi
  • Organizer: Commemorative International Symposium of the Japan Society of Nucleic Acids Chemistry
  • Int'l Joint Research
• [Presentation] Heme Meets DNA. (2019)
  • Author(s): Y. Yamamoto, R. Shinomiya, H. Kotani, H. Araki, A. Momotake, T. Shibata, S. Yanagisawa, T. Ogura, H. Hemmi, A. Suzuki, S. Neya, and T. Kojima
  • Organizer: 19th International Conference on Biological Inorganic Chemistry (ICBIC-19)
  • Int'l Joint Research / Invited
• [Presentation] DNAzyme Possessing Heme (2019)
  • Author(s): Y. Yamamoto, R. Shinomiya, H. Araki, T. Shibata, A. Momotake, S. Yanagisawa, T. Ogura, A. Suzuki, and S. Neya
  • Organizer: The 46th International Symposium on Nucleic Acids Chemistry
  • Int'l Joint Research
• [Presentation] Enhancement of Peroxidase Activity of Heme-DNAzyme through Interaction with Cationic Copolymers (2019)
  • Author(s): H. Araki, A. Momotake, Y. Yamamoto, T. Ochiai, N. Shimada, and A. Maruyama
  • Organizer: The 46th International Symposium on Nucleic Acids Chemistry
  • Int'l Joint Research
• [Presentation] Complexes between G-quadruplex DNA and Phthalocyanines (2019)
  • Author(s): M. Uchiyama, T. Shibata, A. Momotake, T. Ikeue, and Y. Yamamoto
  • Organizer: The 46th International Symposium on Nucleic Acids Chemistry
  • Int'l Joint Research
• [Presentation] Electronic Control of Myoglobin Function (2019)
  • Author(s): T. Sugita, T. Matsuo, S. Hirota, S. Yanagisawa, T. Ogura, O. Shoji, Y. Watanabe, A. Suzuki, S. Neya, A. Momotake, and Y. Yamamoto
  • Organizer: 17th International Symposium on Blood Substitutes & Oxygen Therapeutics (XVII-ISBS-2019)
  • Int'l Joint Research
• [Presentation] ヘムをもつ DNA 酵素 (2019)
  • Author(s): 山本泰彦、 篠宮僚介、小谷弘明、 荒木はるか、柴田友和、 百武篤也、 柳澤幸子、小倉尚志、逸見光、鈴木秋弘、根矢三郎、小島隆彦
  • Organizer: 第46回生体分子科学討論会
• [Presentation] 非線形光学イメージングに向けた無蛍光性分子プローブの開発と応用 (2019)
  • Author(s): 百武篤也、水口高翔、塗谷睦生、安井正人、山本泰彦
  • Organizer: 第46回生体分子科学討論会
• [Presentation] 四重鎖 DNA の形成様式がヘムをもつ DNA 酵素の性質に与える影響 (2019)
  • Author(s): 荒川和基、山田果央、片平裕弥、越智健太郎、中山優作、荒木はるか、篠宮僚介、百武篤也、山本泰彦
  • Organizer: 第46回生体分子科学討論会
• [Presentation] 3'末端に I-カルテットをもつ四重鎖 DNA とヘムの複合体の研究 (2019)
  • Author(s): 菅原綾、篠宮僚介、木下真志、柴田友和、百武篤也、山本泰彦、 柳澤幸子、小倉尚志、鈴木秋弘、根矢三郎
  • Organizer: 第46回生体分子科学討論会
• [Presentation] ヘム鉄の軸配位子としてイミダゾールをもつミオグロビンの研究 (2019)
  • Author(s): 杉田達郎、百武篤也、山本泰彦、松尾貴史、廣田俊、鈴木秋弘、根矢三郎、荘司長三、渡辺芳人
  • Organizer: 第46回生体分子科学討論会
• [Presentation] ヘムをもつDNA 酵素の酸化触媒機構 (2019)
  • Author(s): 山本泰彦、篠宮僚介、小谷弘明、荒木はるか、柴田友和、百武篤也、柳澤幸子、小倉尚志、鈴木秋弘、根矢三郎、小島隆彦
  • Organizer: 第13回バイオ関連化学シンポジウム2019
• [Presentation] Structural characterization of deoxyribozyme possessing heme (2019)
  • Author(s): Y. Yamamoto, Y. Suzuki, Y. Nakajima, T. Shibata, A. Momotake, S. Neya, and A. Suzuki
  • Organizer: 第58回NMR討論会(2019)
• [Presentation] 四重鎖DNAとヘムの複合体の酸化触媒機構 (2019)
  • Author(s): 菅原綾、篠宮僚介、小谷弘明、荒木はるか、柴田友和、百武篤也、柳澤幸子、小倉尚志、鈴木秋弘、根矢三郎、小島隆彦、山本泰彦
  • Organizer: 第52回酸化反応討論会
• [Presentation] 四重鎖DNAの形成様式がヘムをもつDNA酵素の性質に与える影響 (2019)
  • Author(s): 山田果央、荒川和基、篠宮僚介、荒木はるか、柴田友和、百武篤也、山本泰彦
  • Organizer: 日本化学会 第99春季年会 (2019)
• [Presentation] ヘムをもつDNA酵素のヘム鉄に軸配位子として結合する水分子の性質 (2019)
  • Author(s): 山本泰彦、篠宮僚介、荒木はるか、柴田友和、百武篤也、柳澤幸子、小倉尚志、鈴木秋弘、根矢三郎、逸見光
  • Organizer: 日本化学会 第99春季年会 (2019)
• [Presentation] ヘムをもつDNA酵素におけるペルオキシダーゼ活性発現機構の解明 (2019)
  • Author(s): 篠宮僚介、百武篤也、柳澤幸子、鈴木秋弘、根矢三郎、小谷弘明、小島隆彦、Sen Dipankar、山本泰彦
  • Organizer: 日本化学会 第99春季年会 (2019)
• [Presentation] Redox-catalyzing Deoxyribozymes Composed of Heme and DNAs (2018)
  • Author(s): Y. Yamamoto, H. Araki, R. Shinomiya, K. Hayasaka, T. Shibata, A. Momotake, S. Yanagisawa, T. Ogura, H. Hemmi, A. Suzuki, and S. Neya
  • Organizer: 9th Asian Biological Inorganic Chemistry Conference (AsBiC9)
• [Presentation] Deoxyribozymes Composed of Heme and G-quadruplex DNAs (2018)
  • Author(s): Y. Yamamoto, H. Araki, M. Uchiyama, K. Hayasaka, R. Shinomiya, T. Shibata, A. Momotake, M. Hagihara, and H. Hemmi
  • Organizer: Advances in Noncanonical Nucleic Acids 2018 (ANNA2018)
• [Presentation] ヒトテロメア類似塩基配列の四重鎖DNAとヘムとの複合体の構造と機能の解析 (2018)
  • Author(s): 荒木はるか、篠宮僚介、柴田友和、逸見光、萩原正規、小倉尚志、柳澤幸子、鈴木秋弘、根矢三郎、Sen Dipankar、山本泰彦
  • Organizer: 第12回バイオ関連化学シンポジウム2018
• [Presentation] Structure-function investigation of deoxyribozymes composed of heme and G-quadruplex DNAs (2018)
  • Author(s): Y. Yamamoto, H. Araki, K. Hayasaka, R. Shinomiya, Y. Nakayama, K. Ochi, T. Shibata, A. Momotake, M. Hagihara, D. Sen, and H. Hemmi
  • Organizer: 第57回NMR討論会(2018)
• [Presentation] キメラ塩基配列d(TTA)r(GGG)d(T)の四重鎖とヘムの複合体 (2018)
  • Author(s): 早坂 公佑、柴田 友和、松井 亨、山本 泰彦、逸見 光、石塚 匠、徐 岩
  • Organizer: 第20回 RNA学会年会 (RNA2018 OSAKA)
• [Presentation] ヘムの化学修飾がミオグロビンの酸化還元電位に与える影響 (2018)
  • Author(s): 中村 朝香、中村 俊平、柴田 友和、山本 泰彦、鈴木 秋弘、根矢 三郎、三重 安弘
  • Organizer: 第45回生体分子科学討論会
• [Presentation] Characterization of Peroxidase Activities and Structures of Complexes between Chemically Modified Hemes and G-quadruplex DNA Formed from d(TTAGGG) (2018)
  • Author(s): R. Shinomiya, T. Shibata, S. Yanagisawa, T. Ogura, S. Neya, A. Suzuki, and Y. Yamamoto
  • Organizer: 43rd International Conference on Coordination Chemistry (ICCC2018)
• [Presentation] Characterization of Catalytic Activities and Heme Coordination Structures of Heme-DNA complexes Composed of Chemically-modified Hemes and an All Parallel G-quadruplex DNA [d(TTAGGG)]4 (2018)
  • Author(s): R. Shinomiya, T. Shibata, S. Yanagisawa, T. Ogura, S. Neya, A. Suzuki, and Y. Yamamoto
  • Organizer: Tsukuba Global Science Week 2018 (TGSW2018)
• [Presentation] Characterization of G-quadruplex formed from Chimeric Sequence d(TTA)r(GGG)d(T) and its Complex with Heme (2018)
  • Author(s): K. Hayasaka, T. Shibata, T. Matsui, Y. Yamamoto, H. Hemmi, T. Ishizuka, and Y. Xu
  • Organizer: Tsukuba Global Science Week 2018 (TGSW2018)
• [Presentation] Structural Characterization of G-Quadruplex DNAs Formed from Human Telomere-Related Sequences and Their Complexes with Heme (2017)
  • Author(s): Y. Yamamoto, K. Ochi, T. Shibata, H. Hemmi, and M. Hagihara
  • Organizer: 58th Experimental Nuclear Magnetic Resonance Conference (ENC)
  • Place of Presentation: Asilomar Conference Center (California, USA)
  • Year and Date: 2017-03-26
• [Presentation] DNA塩基配列がヘム-DNA複合体の構造と機能に与える影響 (2017)
  • Author(s): 片平祐弥，柴田友和, 松井亨, 守橋健二, 渡部明莉, 中尾知美, 柳澤幸子, 小倉尚志, 山本泰彦
  • Organizer: 日本化学会 第97春季年会(2017)
  • Place of Presentation: 慶応義塾大学日吉キャンパス（神奈川県横浜市）
  • Year and Date: 2017-03-16
• [Presentation] 種々のフッ素化ヘムと四重鎖DNA[d(TTAGGG)]4の複合 体の機能と構造 (2017)
  • Author(s): 篠宮僚介, 片平祐弥, 柴田友和, 小倉尚志, 柳澤幸 子, 中尾知美, 鈴木秋弘, 山本泰彦
  • Organizer: 日本化学会 第97春季年会(2017)
  • Place of Presentation: 慶応義塾大学日吉キャンパス（神奈川県横浜市）
  • Year and Date: 2017-03-16
• [Presentation] ヘムの系統的化学修飾および遠位アミノ酸置換がミオグロビンの機能に与える影響 (2017)
  • Author(s): 渡邉美帆, 金井佑生, 西村龍, 柴田友和, 松尾貴史, 廣田俊, 柳澤幸子, 小倉尚志, 鈴木秋弘, 根矢三郎, 山本泰彦
  • Organizer: 日本化学会 第97春季年会(2017)
  • Place of Presentation: 慶応義塾大学日吉キャンパス（神奈川県横浜市）
  • Year and Date: 2017-03-16
• [Presentation] デオキシミオグロビンとデオキシ西洋わさびペルオキシダーゼにおけるヘムの電子状態の比較 (2017)
  • Author(s): 並木孝介, 柴田友和, 鈴木秋弘, 根矢三郎, 山本泰彦
  • Organizer: 日本化学会 第97春季年会(2017)
  • Place of Presentation: 慶応義塾大学日吉キャンパス（神奈川県横浜市）
  • Year and Date: 2017-03-16
• [Presentation] ヘムと四重鎖DNAの複合体の構造解析 (2017)
  • Author(s): 越智健太郎, 山本泰彦, 柴田友和, 萩原正規, 逸見光
  • Organizer: 日本化学会 第97春季年会(2017)
  • Place of Presentation: 慶応義塾大学日吉キャンパス（神奈川県横浜市）
  • Year and Date: 2017-03-16
• [Presentation] Characterization of peroxidase cycle through a change in electron density of heme atom (2017)
  • Author(s): 1.Kosuke Namiki, Tomokazu Shibata, Saburo Neya, Akihiro Suzuki, and Yasuhiko Yamamoto
  • Organizer: 6th Georgian Bay International Conference on Bioinorganic Chemistry
  • Int'l Joint Research
• [Presentation] Heme-DNA Complexes (2017)
  • Author(s): Yasuhiko Yamamoto, Tomokazu Shibata, Yuya Katahira, Kentaro Ochi, Yusaku Nakayama, Hulin Tai, Akari Watanabe, Tomomi Nakao, Sachiko Yanagisawa, Takashi Ogura, Hikaru Hemmi, Masaki Hagihara, Akihiro Suzuki, and Saburo Neya
  • Organizer: 14th International Symposium on Applied Bioinorganic Chemistry(ISABC14)
  • Int'l Joint Research
• [Presentation] ミオグロビンにおけるヘム配向の決定機構の解明 (2017)
  • Author(s): 中村俊平、金井佑生、原田彩佳、柴田友和、西村龍、並木孝介、渡邉美帆、湯本史明、千田俊哉、鈴木秋弘、根矢三郎、山本泰彦
  • Organizer: 第44回生体分子科学討論会
• [Presentation] ヘムの電子構造および遠位アミノ酸変異がミオグロビンの機能調節に与える影響 (2017)
  • Author(s): 渡邉美帆、金井佑生、西村龍、柴田友和、松尾貴史、廣田俊、柳澤幸子、小倉尚志、鈴木秋弘、根矢三郎、山本泰彦
  • Organizer: 錯体化学会 第67回討論会
• [Presentation] ミオグロビンのオキシ体における共鳴混成体の解析 (2017)
  • Author(s): 長谷川和康、齋藤真器名、瀬戸誠、小林康浩、太田雄大、柳澤幸子、小倉尚志、山本泰彦、柴田友和、根矢三郎、鈴木秋弘
  • Organizer: 錯体化学会 第67回討論会
• [Presentation] Heme as a Prosthetic Group of DNAzymes (2017)
  • Author(s): Yasuhiko Yamamoto
  • Organizer: The First University of Tsukuba and University of Grenoble-Alpes Joint Symposium
  • Int'l Joint Research / Invited
• [Presentation] ヘム軸配位子ヒスチジンの電子構造における共鳴がミオグロビンの外部配位子識別に与える影響 (2017)
  • Author(s): 山本泰彦、田口耕太朗、鳥澤真鈴、渡邉美帆、西村龍、柴田友和、鈴木秋弘、根矢三郎、逸見光
  • Organizer: 第56回 NMR討論会(2017)
• [Presentation] 四重鎖DNAとヘムが拓く物質科学 (2017)
  • Author(s): 山本泰彦
  • Organizer: エネルギー物質科学研究センター（TREMS）スタートアップミーティング
  • Invited
• [Presentation] 四重鎖DNAとヘムの相互作用が拓く物質科学 (2017)
  • Author(s): 山本泰彦
  • Organizer: 第一回プレ戦略研究会「次世代物質・デバイス戦略開発拠点」
  • Invited
• [Presentation] Characterization of Complexes between heme and G-Quadruplex DNAs Formed from Human Telomere-Related Sequences (2017)
  • Author(s): Tomokazu Shibata, Kentaro Ochi, Haruka Araki, Yusaku Nakayama, Ryosuke Shinomiya, Sachiko Yanagisawa, Takashi Ogura, Hikaru Hemmi, Dipankar Sen, and Yasuhiko Yamamoto
  • Organizer: The second international symposium on biofunctional chemistry(ISBC2017)
• [Presentation] Characterization of Peroxidase Activities and Structures of Complexes, between Chemically Modified Hemes and All Parallel G-Quadruplex DNA, formed from d(TTAGGG) (2017)
  • Author(s): Ryosuke Shinomiya, Tomokazu Shibata, Sachiko Yanagisawa, Takashi Ogura, Akihiro Suzuki, Saburo Neya, Dipankar Sen, and Yasuhiko Yamamoto
  • Organizer: The second international symposium on biofunctional chemistry(ISBC2017)
• [Presentation] ヘムタンパク質の電子論 (2017)
  • Author(s): 山本泰彦
  • Organizer: 生命の機能とかたち、連携セミナーシリーズ第４回
  • Invited
• [Presentation] Characterization of Complexes between Heme and G-quadruplex DNAs (2017)
  • Author(s): Yasuhiko Yamamoto Tomokazu Shibata, Yusaku Nakayama Ryosuke Shinomiya and Haruka Araki
  • Organizer: 2018 Joint Symposium on Energy Materials Science and Technology (Workshop of Pre-Strategic Initiatives)
  • Invited
• [Presentation] Characterization of Peroxidase Activities and Heme Coordination Structures of Complexes between Chemically Modified Hemes and G-quadruplex DNA formed from d(TTAGGG) (2017)
  • Author(s): Ryosuke Shinomiya, Tomokazu Shibata, Takashi Ogura, Sachiko Yanagisawa, Akihiro Suzuki, Saburo Neya, Dipankar Sen, and Yasuhiko Yamamoto
  • Organizer: 2018 Joint Symposium on Energy Materials Science and Technology (Workshop of Pre-Strategic Initiatives)
• [Presentation] Characterization of Heme Orientational Disorder in a Myoglobin Reconstituted with a fluorinated Heme Cofactor (2017)
  • Author(s): Shunpei Nakamura, Yuki Kanai, Ayaka Harada, Tomokazu Shibata, Ryu Nishimura, Fumiaki Yumoto, Toshiya Senda, Akihiro Suzuki, Saburo Neya, and Yasuhiko Yamamoto
  • Organizer: 2018 Joint Symposium on Energy Materials Science and Technology (Workshop of Pre-Strategic Initiatives)
• [Presentation] 化学修飾ヘムと四重鎖DNA [d(TTAGGG)]4 の複合体のペルオキシダーゼ活性およびヘム配位構造の解析 (2017)
  • Author(s): 篠宮僚介, 柴田友和, 小倉尚志, 柳澤 幸子, 鈴木秋弘, 根矢三郎, Sen Dipankar, 山本泰彦
  • Organizer: 日本化学会 第98春季年会 (2018)
• [Presentation] ヒトテロメア類似塩基配列の四重鎖DNAとヘム複合体のNMR分光法による構造解析 (2017)
  • Author(s): 山本泰彦, 荒木はるか, 中山優作, 越智健太郎, 柴田友和, 逸見光, 萩原正規、Sen Dipankar
  • Organizer: 日本化学会 第98春季年会 (2018)
• [Presentation] ヒトテロメア類似塩基配列の四重鎖DNAとヘム複合体のペルオキシダーゼ活性と構造の関係の解析 (2017)
  • Author(s): 荒木はるか, 中山優作, 越智健太郎, 篠宮僚介, 柴田友和, 逸見光, 萩原正規, 小倉尚志, 柳澤幸子, 鈴木秋弘, 根矢三郎、Sen Dipankar, 山本泰彦
  • Organizer: 日本化学会 第98春季年会 (2018)
• [Presentation] Characterization of Complexes between Hemes and Parallel G-Quadruplex DNAs (2016)
  • Author(s): Y. Yamamoto, T. Shibata, Y. Katahira, Y. Nakayama, H. Tai, T. Matsui, K. Morihashi, A. Watanabe, T. Nakao, S. Yanagisawa, T. Ogura, A. Suzuki, S. Neya
  • Organizer: 8th Asian Bioligical Inorganic Chemistry Conference(AsBIC 8)
  • Place of Presentation: The University of Auckland (Auckland, New Zealand)
  • Year and Date: 2016-12-04
  • Int'l Joint Research
• [Presentation] ヘムと四重鎖DNAの複合体の構造解析 (2016)
  • Author(s): 越智健太郎, 柴田友和, 逸見光, 萩原正規, 山本泰彦
  • Organizer: 第55回NMR討論会(2016)
  • Place of Presentation: 広島国際会議場（広島県広島市）
  • Year and Date: 2016-11-16
• [Presentation] Structural characterization of Catalytic DNAs Composed of Heme and Parallel G-quadruplexes (2016)
  • Author(s): Katahira, T. Shibata, T. Matsui, K. Morihashi, A. Watanabe, T. Nakao, S. Yanagisawa, T. Ogura, and Y. Yamamoto
  • Organizer: 第43回国際核酸化学シンポジウム(ISNAC 2016)
  • Place of Presentation: 熊本大学工学部百周年記念館（熊本県熊本市）
  • Year and Date: 2016-09-27
  • Int'l Joint Research
• [Presentation] Effects of DNA Sequence Alteration on Structure of a Complex between Heme and All-Parallel G-Quadruplex (2016)
  • Author(s): T. Shibata, Y. Nakano, Y. Moritaka, Y. Nakayama, Y. Katahira, H. Tai, and ○Y. Yamamoto
  • Organizer: 第43回国際核酸化学シンポジウム(ISNAC 2016)
  • Place of Presentation: 熊本大学工学部百周年記念館（熊本県熊本市）
  • Year and Date: 2016-09-27
  • Int'l Joint Research
• [Presentation] ヘムと平行型四重鎖DNAの複合体の構造および機能の解析 (2016)
  • Author(s): 柴田 友和，片平 祐弥，山本 泰彦
  • Organizer: 第17回若手NMR研究会
  • Place of Presentation: 箱根高原ホテル（神奈川県足柄下郡箱根町）
  • Year and Date: 2016-09-10
• [Presentation] ヘムと四重鎖DNAの複合体の触媒機能測定と構造解析 (2016)
  • Author(s): 山本 泰彦，片平 祐弥，中山 優作，柴田 友和，渡部 明莉，中尾 知美，柳澤 幸子，小倉 尚志，鈴木 秋弘，根矢 三郎
  • Organizer: 第10回バイオ関連化学シンポジウム
  • Place of Presentation: 石川県立音楽堂（石川県金沢市）
  • Year and Date: 2016-09-07
• [Presentation] Structural and Functional Characterization of Complexes between Heme and G-quadruplex DNAs Formed from Human Telomere-related Sequences (2016)
  • Author(s): T. Shibata, Y. Katahira, H. Hemmi, and Y. Yamamoto
  • Organizer: The 27th International Conference on Magnetic Resonance in Biological Systems(ICMRBS2016)
  • Place of Presentation: 京都国際会館（京都府京都市）
  • Year and Date: 2016-08-21
  • Int'l Joint Research
• [Book] Experimental Approaches of NMR Spectroscopy, Methodology and Application to Life Science and Materials Science (2018)
  • Author(s): Akira Naito, Tetsuo Asakura, Ichio Shimada, Kiyonori Takegoshi, and Yasuhiko Yamamoto
  • Total Pages: 636
  • Publisher: Springer
  • ISBN: 9789811059650
• [Remarks] 筑波大学 数理物質系 生物無機化学研究室
  • URL: http://www.chem.tsukuba.ac.jp/yamamoto/
• [Remarks] http://www.chem.tsukuba.ac.jp/yamamoto/