| Budget Amount *help |
¥18,200,000 (Direct Cost: ¥14,000,000、Indirect Cost: ¥4,200,000)
Fiscal Year 2018: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2017: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2016: ¥8,190,000 (Direct Cost: ¥6,300,000、Indirect Cost: ¥1,890,000)
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| Outline of Final Research Achievements |
Oxygen-evolving Photosystem II (PSII) protein in photosynthetic organisms has a Mn4CaO5 cluster as the reaction center of catalyzing a water-splitting reaction. In this study, the oxidation state in each of four Mn atoms in the cluster was investigated as well as the crystal structure of PSII in the four intermediate states of the reaction. To understand the detailed process of the water-splitting reaction in PSII, we developed a novel crystallographic analysis method using the wavelength of the absorption inflection point of a target metal atom. We prepared a lot of tinny PSII crystal, excited by pulse-laser, and then cryo-fixed. Both redox properties of each Mn atom in the cluster and crystal structure of PSII were simultaneously analyzed. As a result, we observed the redox changes in each Mn atom in the process of water-splitting reaction. Simultaneously with this redox analysis, we also captured the movements of water and proteins in PSII in the process of PSII.
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