| Budget Amount *help |
¥18,330,000 (Direct Cost: ¥14,100,000、Indirect Cost: ¥4,230,000)
Fiscal Year 2018: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2017: ¥6,760,000 (Direct Cost: ¥5,200,000、Indirect Cost: ¥1,560,000)
Fiscal Year 2016: ¥6,890,000 (Direct Cost: ¥5,300,000、Indirect Cost: ¥1,590,000)
|
|---|
| Outline of Final Research Achievements |
During cell division, the animal cell assembles the contractile actomyosin ring to separate the cell body into two daughter cells. To elucidate the assembly mechanism of the contractile ring, we purified six key components of the contractile ring including actin, myosin, and formin from living cells. Then, we performed single-molecule manipulation experiments and in vitro reconstitution experiments to address following two questions: 1) How the force generated by myosin molecules modulates the actin polymerization activity of formin transmitted through the polymerizing actin filament. 2) How the force orchestrates proteins to regulate the contractile ring assembly and constriction. Our findings from this project will help us understand the regulatory mechanism of the cytokinetic ring dynamics in living cells.
|
