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Optical control of transition states in proteins with mid-infrared laser pulses at high intensities

Research Project

Project/Area Number 16KT0163
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section特設分野
Research Field Transition State Control
Research InstitutionSaitama University (2019)
Osaka University (2016-2018)

Principal Investigator

Nakamura Ryosuke  埼玉大学, 理工学研究科, 非常勤研究員 (70379147)

Project Period (FY) 2016-07-19 – 2020-03-31
Project Status Completed (Fiscal Year 2019)
Budget Amount *help
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2018: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Keywords赤外レーザ / 光受容タンパク質 / 赤外レーザー
Outline of Final Research Achievements

Several studies have clarified that the transition among quasi-stable species with their characteristic structures is essential for protein functioning. The purposes of this study are 1) clarification of an essential role of hydrogen-bond network in the transitions; 2) optical control of the transitions with mid-infrared laser pulses.
A femtosecond time-resolved spectroscopy system with high sensitivity was developed based on reaction-trigger UV pulse, tunable mid-infrared pump pulse, and broadband probe pulse. Although a signal change induced by the mid-infrared pulse was clearly observed, it is suggested that the change does not come from the contribution of excited state or intermediate states.

Academic Significance and Societal Importance of the Research Achievements

タンパク質に光を照射することで、その機能性を向上させたり、抑制したり、あるいは本来とは全く異なる機能を発現することができれば、創薬・医療応用に対する新しいストラテジーを描くことができる。本研究では、タンパク質機能の光制御に関して、準安定状態および準安定状態を制御していると予想される水素結合ネットワークに着目した。赤外光によって、水素結合の構造を変調し、それによってタンパク質の機能を制御できるのではないかという仮説を立てた。結果を得るまでには至らなかったが、仮説検証に必要な様々な要素を明らかにすることができ、今後の研究へと展開する素地ができた。

Report

(5 results)
  • 2019 Annual Research Report   Final Research Report ( PDF )
  • 2018 Research-status Report
  • 2017 Research-status Report
  • 2016 Research-status Report
  • Research Products

    (11 results)

All 2018 2017 2016

All Journal Article (4 results) (of which Peer Reviewed: 4 results,  Acknowledgement Compliant: 2 results) Presentation (7 results) (of which Int'l Joint Research: 4 results,  Invited: 1 results)

  • [Journal Article] Polarization sensitive femtosecond mid-infrared spectrometer with tunable Fabry-Perot filter and chirped-pulse upconversion2018

    • Author(s)
      R. Nakamura, Y. Inagaki, T. Kamimura
    • Journal Title

      Applied Optics

      Volume: 57 Issue: 36 Pages: 10517-10521

    • DOI

      10.1364/ao.57.010517

    • Related Report
      2018 Research-status Report
    • Peer Reviewed
  • [Journal Article] Analysis of Resist Removal Phenomenon Using Laser Irradiation2017

    • Author(s)
      T. Kamimura, H. Kuramae, T. Yamashiro, K. Nuno, Y. Umeda, S. Tsujimoto, R. Nakamura, T. Nishiyama, H. Horibe
    • Journal Title

      Journal of Photopolymer Science and Technology

      Volume: 30 Issue: 3 Pages: 291-295

    • DOI

      10.2494/photopolymer.30.291

    • NAID

      130005950263

    • ISSN
      0914-9244, 1349-6336
    • Related Report
      2017 Research-status Report
    • Peer Reviewed
  • [Journal Article] Simultaneous mid-infrared pulse generation and shaping in engineered quasi-phase-matched nonlinear crystals2017

    • Author(s)
      R. Nakamura
    • Journal Title

      Optics Communications

      Volume: 383 Pages: 545-550

    • DOI

      10.1016/j.optcom.2016.09.050

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Wide-bandgap nonlinear crystal LiGaS2 for femtosecond mid-infrared spectroscopy with chirped pulse upconversion2016

    • Author(s)
      R. Nakamura, Y. Inagaki, H. Hata, N. Hamada, N. Umemura, T. Kamimura
    • Journal Title

      Applied Optics

      Volume: 55 Issue: 33 Pages: 9365-9369

    • DOI

      10.1364/ao.55.009365

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Presentation] Polarization Sensitive Femtosecond Mid-Infrared Spectrometer Using Chirped-Pulse Upconversion2018

    • Author(s)
      R. Nakamura, Y. Inagaki, N. Umemura, T. Kamimura
    • Organizer
      Pacific Rim Conference on Lasers and Electro-Optics (CLEO-PR)
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research
  • [Presentation] CuInS2コロイドの可視・中赤外時間分解分光2018

    • Author(s)
      畑寛明, 向井駿, 東本慎也, 中村亮介
    • Organizer
      第79回応用物理学会秋季学術講演会
    • Related Report
      2018 Research-status Report
  • [Presentation] レーザー照射を用いたレジスト剥離現象の解析2017

    • Author(s)
      神村共住,布晃輔,山城鷹之,梅田悠史,辻本慎吾,倉前宏行,中村亮介,西山聖,堀邊英夫
    • Organizer
      The 34th International Conference of Photopolymer Science and Technology
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] Damage resistance of wide-bandgap nonlinear crystals for femtosecond mid-infrared spectrometer using chirped-pulse upconversion2016

    • Author(s)
      M. Akimoto, Y. Inagaki, H. Hata, N. Hamada, R. Nakamura, N. Umemura, M. Yoshimura, and T. Kamimura
    • Organizer
      48th Annual Symposium on Optical Materials for High-Power Lasers
    • Place of Presentation
      Boulder, CO, USA
    • Year and Date
      2016-09-25
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research
  • [Presentation] Laser removal of PVP without causing laser-induced surface damage2016

    • Author(s)
      K. Nuno, T. Yamashiro, S. Tuzimoto, R. Nakamura, S. Takagi, T. Nishiyama, H. Horibe, and T. Kamimura
    • Organizer
      48th Annual Symposium on Optical Materials for High-Power Lasers
    • Place of Presentation
      Boulder, CO, USA
    • Year and Date
      2016-09-25
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research
  • [Presentation] 和周波混合を用いた周波数領域2次元赤外分光法の開発II2016

    • Author(s)
      稲垣嘉清, 畑秀文, 神村共住, 梅村信弘, 濱田格雄, 中村亮介
    • Organizer
      日本物理学会2016年秋季大会
    • Place of Presentation
      金沢大学
    • Year and Date
      2016-09-13
    • Related Report
      2016 Research-status Report
  • [Presentation] リアルタイム時系列光子計測によるタンパク質のミリ秒構造追跡2016

    • Author(s)
      北林和樹, 神村共住, 濱田格雄, 中村亮介
    • Organizer
      日本物理学会2016年秋季大会
    • Place of Presentation
      金沢大学
    • Year and Date
      2016-09-13
    • Related Report
      2016 Research-status Report

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Published: 2016-07-20   Modified: 2023-03-08  

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