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Establishment of planar polarity for plant cell morphogenesis

Research Project

Project/Area Number 16KT0170
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section特設分野
Research Field Constructive Systems Biology
Research InstitutionKyoto University

Principal Investigator

Aoyama Takashi  京都大学, 化学研究所, 教授 (80202498)

Research Collaborator KATO Mariko  
YASUDA Keiko  
WATARI Machiko  
KISHIMOTO Taichi  
Project Period (FY) 2016-07-19 – 2019-03-31
Project Status Completed (Fiscal Year 2018)
Budget Amount *help
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2018: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Keywords細胞極性 / 細胞形態形成 / 植物細胞 / リン脂質シグナル / PIP5K / PIP2 / 低分子Gタンパク質 / ROP / ROP GTPase / 植物細胞形態形成 / ROP-GTPase / 植物 / 発生・分化 / シグナル伝達 / 脂質 / 蛋白質
Outline of Final Research Achievements

During root hair development of Arabidopsis thaliana, signaling factors including ROP2 and PIP5K3 are assumed to be involved in the mechanism that establishes the planar polarity for bulge formation. In this study, to elucidate the molecular basis of the mechanism, we performed in silico simulation of a model, in which ROP2, PIP5K3 and PI(4,5)P2 constitute a feedforward loop, and in planta constructive experiments for its validation. We expressed fluorescence protein-fused ROP2 and PIP5K3 using the GL2 promoter, which is active specifically in atrichoblasts. When they were expressed separately, they did not show local accumulation patterns like that observed in trichoblasts. Moreover, their co-expression did not influence their localization patterns to each other. As for interaction between ROP2 and PI(4,5)P2, co-localization of the ROP2 fusion protein with PI(4,5)P2 on the plasma membrane was suggested.

Academic Significance and Societal Importance of the Research Achievements

根毛や花粉管、コケ類の原糸体や仮根などの細胞先端成長においては、伸長極性の確立・維持機構としてLocalization Enhancing Network Self-sustaining (LENS)というものが提唱されている。根毛バルジ形成のために確立された平面内極性は先端成長極性として維持されることから、本研究は細胞先端成長の基盤となるLENSの実態解明につながるものと期待される。また、本研究のフィードフォワードループモデルにおけるPI(4,5)P2の直接的または間接的なROPのリクルートの可能性は、動物細胞系における同様の作業仮説の設定において有用な情報であると考えられる。

Report

(4 results)
  • 2018 Annual Research Report   Final Research Report ( PDF )
  • 2017 Research-status Report
  • 2016 Research-status Report
  • Research Products

    (16 results)

All 2019 2018 2017 2016 Other

All Int'l Joint Research (1 results) Journal Article (4 results) (of which Int'l Joint Research: 2 results,  Peer Reviewed: 4 results,  Open Access: 2 results) Presentation (11 results) (of which Int'l Joint Research: 2 results,  Invited: 1 results)

  • [Int'l Joint Research] 北京大学(中国)

    • Related Report
      2016 Research-status Report
  • [Journal Article] Arabidopsis PCaP2 modulates the phosphatidylinositol 4,5-bisphosphate signal on the plasma membrane and attenuates root hair elongation2019

    • Author(s)
      Kato Mariko、Tsuge Tomohiko、Maeshima Masayoshi、Aoyama Takashi
    • Journal Title

      The Plant Journal

      Volume: in press Issue: 4 Pages: 610-625

    • DOI

      10.1111/tpj.14226

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] PtdIns (3, 5) P 2 mediates root hair shank hardening in Arabidopsis2018

    • Author(s)
      Tomoko Hirano, Hiroki Konno, Seiji Takeda, Liam Dolan, Mariko Kato, Takashi Aoyama, Takumi Higaki, Hisako Takigawa-Imamura, Masa H Sato
    • Journal Title

      Nature Plants

      Volume: 11 Issue: 4 Pages: 447-447

    • DOI

      10.1038/s41477-019-0416-x

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] SRPP, a cell-wall protein is involved in development and protection of seeds and root hairs in Arabidopsis thaliana2017

    • Author(s)
      Natsuki Tanaka, Hiroshi Uno, Shohei Okuda, Shizuka Gunji, Ali Ferjani, Takashi Aoyama, Masayoshi Maeshima
    • Journal Title

      Plant Cell Physiology

      Volume: 58 Pages: 760-769

    • DOI

      10.1093/pcp/pcx008

    • NAID

      120006343667

    • Related Report
      2017 Research-status Report 2016 Research-status Report
    • Peer Reviewed
  • [Journal Article] RNA binding proteins RZ-1B and RZ-1C play critical roles in regulating pre-mRNA splicing and gene expression during development in Arabidopsis.2016

    • Author(s)
      Wu Z, Zhu D, Lin X, Miao J, Gu L, Deng X, Yang Q, Sun K, Zhu D, Cao X, Tsuge T, Dean C, Aoyama T, Gu H, Qu LJ.
    • Journal Title

      Plant Cell

      Volume: 28 Issue: 1 Pages: 55-73

    • DOI

      10.1105/tpc.15.00949

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Presentation] PtdIsn(4,5)P2産生酵素をコードするPIP5K遺伝子は花粉の発達過程において機能する2019

    • Author(s)
      加藤真理子、亘真智子、藤原崇志、青山卓史
    • Organizer
      第60回日本植物生理学会年会
    • Related Report
      2018 Annual Research Report
  • [Presentation] シロイヌナズナの根毛形成における平面内極性確立機構の解明2019

    • Author(s)
      岸本太地、加藤真理子、柘植知彦、青山卓史
    • Organizer
      第60回日本植物生理学会年会
    • Related Report
      2018 Annual Research Report
  • [Presentation] シロイヌナズナにおけるPIP5K7とPIP5K8の機能解析2019

    • Author(s)
      黒田凌、加藤真理子、柘植知彦、青山卓史
    • Organizer
      第60回日本植物生理学会年会
    • Related Report
      2018 Annual Research Report
  • [Presentation] シロイヌナズナPIP5K7およびPIP5K8の機能解析2018

    • Author(s)
      黒田凌、加藤真理子、柘植知彦、青山卓史
    • Organizer
      第31回植物脂質シンポジウム
    • Related Report
      2018 Annual Research Report
  • [Presentation] Genetic analysis of type B PIP5Ks in the plant cell morphogenesis2018

    • Author(s)
      Watari Machiko, Kato Mariko, Tsuge Tomohiko, Aoyama Takashi
    • Organizer
      The 29th International Conference of Arabidopsis Research
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Functions of Arabidopsis type-B phosphatidylinositol phosphate 5-kinase genes in plant growth and development2018

    • Author(s)
      Kato Mariko, Watari Machiko, Kuroda Ryo, Kishimoto Taichi, Kusano Hiroaki, Tsuge Tomohiko, Romain Blanc-Mathieu, Ogata Hiroyuki, Aoyama Takashi
    • Organizer
      The 23rd International Symposium on Plant Lipids
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Biological function of type-B phosphatidylinositol phosphate 5-kinase genes of Arabidopsis thaliana.2017

    • Author(s)
      Takashi Aoyama, Mariko Kato, Yukika Wada, Machiko Watari, Tomohiko Tsuge, Blanc-Mathieu Romain, Hiroyuki Ogata, Hiroaki Kusano
    • Organizer
      日本植物生理学会・2017年度年会
    • Place of Presentation
      鹿児島大学
    • Year and Date
      2017-03-16
    • Related Report
      2016 Research-status Report
    • Invited
  • [Presentation] シロイヌナズナPCaP2はPI(4,5)P2シグナルを調節することにより根毛の伸長を減衰する2017

    • Author(s)
      加藤真理子、柘植知彦、前島正義、青山卓史
    • Organizer
      日本植物生理学会・2018年度年会
    • Related Report
      2017 Research-status Report
  • [Presentation] シロイヌナズナにおけるPIP5K7とPIP5K8の機能解析2017

    • Author(s)
      黒田凌、加藤真理子、柘植知彦、青山卓史
    • Organizer
      日本植物生理学会・2018年度年会
    • Related Report
      2017 Research-status Report
  • [Presentation] BタイプPIP5Kの遺伝学的解析2017

    • Author(s)
      亘真智子、加藤真理子、柘植知彦、青山卓史
    • Organizer
      日本植物生理学会・2018年度年会
    • Related Report
      2017 Research-status Report
  • [Presentation] シロイヌナズナPLDz1およびPLDz2の細胞内局在2017

    • Author(s)
      島村亮太、谷口(山本)幸美、加藤真理子、柘植知彦、青山卓史
    • Organizer
      日本植物生理学会・2018年度年会
    • Related Report
      2017 Research-status Report

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Published: 2016-07-20   Modified: 2023-03-08  

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