| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2018: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2017: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Outline of Final Research Achievements |
This study aimed to clarify the mechanisms regulating the autonomous neuronal circuit formation, especially focusing on the orthogonal interaction between Purkinje cell dendrites and granule cell axons in the cerebellar circuits. We showed that Spectrin beta III is essential for the perpendicular dendrite formation with respect to axonal fibers. The molecule forms regular periodic structures in the Purkinje cell dendrites and their filopodial base by using super-resolution microscopy STED. We also found that Spectrin beta III regulated the microtubule dynamics in the dendritic area. In the absence of Spectrin beta III, microtubule polymerized into the filopodial region more frequently than control, which resulted in the abnormal extension of filopodia in the wrong direction. Our results demonstrated that Spectrin beta III plays a pivotal role in the regulation of dendritic growth orientation during cerebellar circuit formation.
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