Elucidation of the pathogenic mechanism of progression of Parkinson's disease using a primate genetic modification model

• Project/Area Number: 16KT0193
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 特設分野
• Research Field: Complex Systems Disease Theory
• Research Institution: Kyoto University
• Principal Investigator: Inoue Kenichi 京都大学, 霊長類研究所, 助教 (90455395)
• Research Collaborator: TAKATA MASAHIKO ; NINOMIYA TAIHEI
• Project Period (FY): 2016-07-19 – 2019-03-31
• Project Status: Completed (Fiscal Year 2018)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2018: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: 神経科学 / 脳神経疾患 / パーキンソン / ウイルスベクター / 霊長類 / ウィルスベクター

Outline of Final Research Achievements

In the present study, by using viral vectors, we developed a novel macaque Parkinson's disease model (alpha-synuclein overexpression model) in which progressive neurodegeneration persisted for more than several months. We also developed a tyrosine hydroxylase promoter with relatively high selectivity for dopamine cells and efficient transgene expression. In addition, we have developed and performed various analyses of Parkinson's disease models, including behavioral, histological, and biochemical analyses, as well as brain function image and electrophysiological analyses. In these experiments, we have obtained some new findings about the the pathogenic mechanism of progression of Parkinson's disease, such as changes in the concentrations of various enzymes in cerebrospinal fluid, the dynamics of oscillation in the brain including synchronization between fields, and the inhibitory effect of calcium-binding protein on the progression of the disease.

Academic Significance and Societal Importance of the Research Achievements

症状発症時には８割もの黒質ドーパミン神経細胞が細胞死を起こしているパーキンソン病の根治治療には、無症状だが脳内で病態が進行している状態を検出し、その進行を止めるための治療を行うことが極めて重要となる。ヒトの病態進行と近い進行性の黒質ドーパミン細胞の神経変性を示すマカクザルパーキンソン病モデルの開発という本研究の成果は、パーキンソン病の病態機序の理解に大きく寄与できるほか、このモデル動物において経時的に本研究で一部開発した様々な解析法によるデータを取得することにより、病態の進行様式や疾病の発症機序の解明や、病態の段階を検出しうるマーカーの開発が進むことが期待される。

Research Products

• [Int'l Joint Research] HM Cinac(スペイン)
• [Journal Article] Enhancement of the transduction efficiency of a lentiviral vector for neuron-specific retrograde gene delivery through the point mutation of fusion glycoprotein type E. (2019)
  • Author(s): Kato S; Sugawara M; Kobayashi K; Kimura K; Inoue K; Takada M; Kobayashi K
  • Journal Title: J Neurosci Methods Volume: 311 Pages: 147-155
  • DOI: 10.1016/j.jneumeth.2018.10.023
  • Peer Reviewed
• [Journal Article] A note on retrograde gene transfer efficiency and inflammatory response of lentiviral vectors pseudotyped with FuG-E vs. FuG-B2 glycoproteins. (2019)
  • Author(s): Tanabe S; Uezono S; Tsuge H; Fujiwara M; Miwa M; Kato S; Nakamura K; Kobayashi K; Inoue K; Takada M
  • Journal Title: Scientific Reports Volume: 9 Issue: 1 Pages: 3567-3567
  • DOI: 10.1038/s41598-019-39535-1
  • NAID: 120006818701
  • Peer Reviewed / Open Access
• [Journal Article] Segmentation of the rabies virus genome (2018)
  • Author(s): Hidaka Yuya、Lim Chang-Kweng、Takayama-Ito Mutsuyo、Park Chun-Ho、Kimitsuki Kazunori、Shiwa Nozomi、Inoue Ken-ichi、Itou Takuya
  • Journal Title: Virus Research Volume: 252 Pages: 68-75
  • DOI: 10.1016/j.virusres.2018.05.017
  • Peer Reviewed / Open Access
• [Journal Article] Causal Role of Neural Signals Transmitted From the Frontal Eye Field to the Superior Colliculus in Saccade Generation (2018)
  • Author(s): Matsumoto Masayuki、Inoue Ken-ichi、Takada Masahiko
  • Journal Title: Frontiers in Neural Circuits Volume: 12 Pages: 69-69
  • DOI: 10.3389/fncir.2018.00069
  • NAID: 120006773079
  • Peer Reviewed / Open Access
• [Journal Article] Recruitment of calbindin into nigral dopamine neurons protects against drug-induced parkinsonism. Mov Disord [Epub ahead of print] doi: 10.1002/mds.107 (2018)
  • Author(s): Inoue K, Miyachi S, Nishi K, Okado H, Nagai Y, Minamimoto T, Nambu A, Takada M
  • Journal Title: Mov Disord Volume: － Issue: 2 Pages: 200-209
  • DOI: 10.1002/mds.107
  • Peer Reviewed
• [Journal Article] Alterations in the reduced pteridine contents in the cerebrospinal fluids of LRRK2 mutation carriers and patients with Parkinson's disease. (2018)
  • Author(s): Ichinose H; Inoue K; Arakawa S; Watanabe Y; Kurosaki H; Koshiba S; Hustad E; Takada M; Aasly JO
  • Journal Title: J Neural Transm Volume: 125 Issue: 1 Pages: 45-52
  • DOI: 10.1007/s00702-017-1784-x
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Multisynaptic projections from the amygdala to the ventral premotor cortex in macaque monkeys: Anatomical substrate for feeding behavior. (2018)
  • Author(s): Ishida H; Inoue K; Takada M
  • Journal Title: Front Neuroanat Volume: 12 Pages: 3-3
  • DOI: 10.3389/fnana.2018.00003
  • Peer Reviewed / Open Access
• [Journal Article] Visuomotor signals for reaching movements in the rostro-dorsal sector of the monkey thalamic reticular nucleus. (2017)
  • Author(s): Saga Y, Nakayama Y, Inoue K, Yamagata T, Hashimoto M, Tremblay L, Takada M, Hoshi E
  • Journal Title: Eur J Neurosci Volume: 印刷中 Issue: 9 Pages: 1186-1199
  • DOI: 10.1111/ejn.13421
  • NAID: 120006305932
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] The use of an optimized chimeric envelope glycoprotein enhances the efficiency of retrograde gene transfer of a pseudotyped lentiviral vector in the primate brain. (2017)
  • Author(s): Tanabe S, Inoue K, Tsuge H, Uezono S, Nagaya K, Fijiwara M, Kato S, Kobayashi K, Takada M
  • Journal Title: Neurosci Res Volume: 印刷中 Pages: 45-52
  • DOI: 10.1016/j.neures.2017.02.007
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] High-speed and scalable whole-brain imaging in rodents and primates (2017)
  • Author(s): Seiriki K, Kasai A, Hashimoto T, Schulze W, Niu M, Yamaguchi S, Nakazawa T, Inoue K, Uezono S, Takada M, Naka Y, Igarashi H, Tanuma M, Waschek JA, Ago Y, Tanaka KF, Hayata-Takano A, Nagayasu K, Shintani N, Hashimoto R, Kunii Y, Hino M, Matsumoto J, Yabe H, Nagai T, Fujita K, Matsuda T, Takuma K, Baba A, Hashimoto H
  • Journal Title: Neuron Volume: 94 Issue: 6 Pages: 1085-1100
  • DOI: 10.1016/j.neuron.2017.05.017
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Pseudotyped lentiviral vectors for retrograde gene delivery into target brain regions. (2017)
  • Author(s): Kobayashi K; Inoue K; Tanabe S; Kato S; Takada M; Kobayashi K
  • Journal Title: Front Neuroanat Volume: 11 Pages: 65-65
  • DOI: 10.3389/fnana.2017.00065
  • Peer Reviewed / Open Access
• [Journal Article] Nonhuman primate optogenetics: Recent advances and future directions. (2017)
  • Author(s): Galvan A; Stauffer WR; Ackerson L; El-Shamayleh Y; Inoue K; Ohayon S; Schmid M.
  • Journal Title: J Neurosci Volume: 37 Issue: 45 Pages: 10894-10903
  • DOI: 10.1523/jneurosci.1839-17.2017
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Propagated but Topologically Distributed Forebrain Neurons Expressing Alpha-Synuclein in Aged Macaque. (2016)
  • Author(s): Kimura K,Inoue K, Kuroiwa Y, Tanaka F, Takada M
  • Journal Title: PLoS ONE Volume: 11 Issue: 11 Pages: e0166861-e0166861
  • DOI: 10.1371/journal.pone.0166861
  • NAID: 120005954462
  • Peer Reviewed / Open Access
• [Journal Article] PET imaging-guided chemogenetic silencing reveals a critical role of primate rostromedial caudate in reward evaluation. (2016)
  • Author(s): Nagai Y, Kikuchi E, Lerchner W, Inoue Ki, Ji B, Eldridge MA, Kaneko H, Kimura Y, Oh-Nishi A, Hori Y, Kato Y, Hirabayashi T, Fujimoto A, Kumata K, Zhang MR, Aoki I, SuharaT, Higuchi M, Takada M, Richmond BJ, Minamimoto T.
  • Journal Title: Nature Communications Volume: 7 Issue: 1 Pages: 13605-13605
  • DOI: 10.1038/ncomms13605
  • NAID: 120005898960
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Presentation] 融合糖タンパク質E型変異体を用いた神経特異的逆行性ウイルスベクターによるマーモセット脳内への遺伝子導入効率の向上. (2019)
  • Author(s): 菅原正晃、加藤成樹、木村 慧、井上 謙一、高田 昌彦、小林 和人
  • Organizer: 第8回日本マーモセット研究会大会
• [Presentation] Retrograde gene transfer efficacy and inflammatory response of HiRet vs. NeuRet lentiviral vectors in the motor cortical input system of nonhuman primates and rodents. (2019)
  • Author(s): Otsuka Y; Tsuge H; Uezono S; Tanabe S; Fujiwara M; Miwa M; Kato S; Nakamura K; Kobayashi K; Inoue K; Takada M
  • Organizer: 第8回日本マーモセット研究会大会
• [Presentation] Retrograde gene transfer efficacy and inflammatory response of lentiviral vectors pseudotyped with FuG-E vs. FuG-B2 glycoproteins in the striatal input system of primates and rodents. (2019)
  • Author(s): Tanabe S; Uezono S; Tsuge H; Fujiwara M; Nagaya K; Nagaya N; Sugawara M; Miwa M; Konoike N; Kato S; Nakamura K; Kobayashi K; Inoue K; Takada M
  • Organizer: 第8回日本マーモセット研究会大会
• [Presentation] ウイルスベクターを利用した霊長類における神経ネットワーク操作 (2019)
  • Author(s): 井上 謙一
  • Organizer: 玉川大学総合人間科学ワークショップ
  • Int'l Joint Research / Invited
• [Presentation] Deep brain stimulation of the ventral midbrain facilitates the output to forelimb muscles via the primary motor cortex in monkeys. (2019)
  • Author(s): Suzuki M; Inoue K; Nakagawa H; Isa T; Takada M; Nishimura Y
  • Organizer: 3rd International Brain Stimulation Conference
  • Int'l Joint Research
• [Presentation] 霊長類脳への神経細胞特異的かつ高効率な遺伝子導入を実現する改変AAVベクターの開発. (2019)
  • Author(s): 木村 慧、永井 裕司、田辺 創思、Zheng Andi、Oh Jungmin、藤原 真紀、中野 真由子、南本 敬史、井上 謙一、高田 昌彦
  • Organizer: 第8回 生理研-霊長研-新潟脳研 合同シンポジウム
• [Presentation] Pathway-selective optogenetics for elucidating neural network function in primates (2019)
  • Author(s): Inoue K
  • Organizer: DFG-AMED joint Workshop New Direction in Systems Neuroscience
  • Int'l Joint Research / Invited
• [Presentation] Neuron-specific efficient gene transduction in the primate brain with modified AAV vectors. (2018)
  • Author(s): Kimura K; Tanabe S; Fujiwara M; Nakano M; Nagai Y; Minamimoto T; Inoue K; Takada M
  • Organizer: 第41回日本神経科学大会
• [Presentation] Lentiviral vectors pseudotyped with FuG-E and FuG-E2 glycoprotein suitable for retrograde gene transfer into neural networks involving cerebral cortical areas in nonhuman primates and rodents. (2018)
  • Author(s): Otsuka Y; Tsuge H; Uezono S; Tanabe S; Fujiwara M; Miwa M; Kato S; Nakamura K; Kobayashi K; Inoue K; Takada M
  • Organizer: 第41回日本神経科学大会
• [Presentation] Using PET imaging to monitor chemogenetic manipulation of nigrostriatal dopamine system in common marmoset. (2018)
  • Author(s): Mimura K; Nagai Y; Inoue K; Suhara T; Takada M; Minamimoto T
  • Organizer: 第41回日本神経科学大会
• [Presentation] PET imaging of selective control of neural activity with a novel DREADD agonist. (2018)
  • Author(s): Nagai Y; Miyakawa N; Huang X; Slocum S; Ono M; Inoue K; Yan X; Liu J; English J; Shimojo M; Mimura K; Hirabayashi T; Bin J; Kumata K; Higuchi M; Zhang M; Takada M; Jin J; Roth B; Minamimoto T
  • Organizer: 第41回日本神経科学大会
• [Presentation] Enhanced gene transfer efficiency of neuron-specific retrograde lentiviral vector with variants of fusion glycoprotein type E into the mouse and common marmoset brains. (2018)
  • Author(s): Sugawara M; Kato S; Kimuta K; Inoue K; Takada M; Kobayashi K
  • Organizer: 第41回日本神経科学大会
• [Presentation] Pathway-selective optogenetic modulation of value-biased saccade circuits in the monkey basal ganglia. (2018)
  • Author(s): Amita H; Hikosaka O; Hyoung F.K; Inoue K; Takada M
  • Organizer: 第41回日本神経科学大会
• [Presentation] Development of viral vectors for delivering functional molecules into nonhuman primate brains. (2018)
  • Author(s): Inoue K
  • Organizer: 第41回日本神経科学大会
  • Invited
• [Presentation] Microarchitecture of binocular disparity processing in V2: combined use of intrinsic optical imaging and 2-photon Ca2+ imaging. (2018)
  • Author(s): Fang Y; Hatanaka G; Inagaki M; Inoue K; Takada M; Fujita I
  • Organizer: International Workshop Monitoring and manipulating brain function in non-human primates
  • Int'l Joint Research
• [Presentation] High-resolution imaging of primate brains using FAST. (2018)
  • Author(s): Hashimoto H; Seiriki K; Kasai A; Nakazawa T; Inoue K; Takada M
  • Organizer: International Workshop Monitoring and manipulating brain function in non-human primates
  • Int'l Joint Research
• [Presentation] Using PET imaging to monitor chemogenetic manipulation of nigrostriatal dopamine system in common marmoset. (2018)
  • Author(s): Mimura K; Nagai Y; Inoue K; Matsumoto J; Suhara T; Nishijo H; Takada M; Minamimoto T
  • Organizer: International Workshop Monitoring and manipulating brain function in non-human primates
  • Int'l Joint Research
• [Presentation] Neuron-specific efficient gene transduction in the primate brain with Morphological modified AAV vectors. (2018)
  • Author(s): Kimura K; Tanabe S; Fujiwara M; Nagai Y; Minamimoto T; Inoue K; Takada M
  • Organizer: International Workshop Monitoring and manipulating brain function in non-human primates
  • Int'l Joint Research
• [Presentation] Differences in retrograde gene transfer efficiency and cytotoxicity between lentiviral vectors pseudotyped with FuG-E; FuG-E2; and FuG-B2 glycoprotein in primate brains. (2018)
  • Author(s): Otsuka Y; Tanabe S; Tsuge H; Uezono S; Fujiwara M; Miwa M; Kato S; Nakamura K; Kobayashi K; Inoue K; Takada M
  • Organizer: International Workshop Monitoring and manipulating brain function in non-human primates
  • Int'l Joint Research
• [Presentation] Development of viral vectors for delivering functional molecules into nonhuman primate brains (2018)
  • Author(s): Inoue K
  • Organizer: International Workshop Monitoring and manipulating brain function in non-human primates
  • Int'l Joint Research
• [Presentation] マーモセットの帯状皮質を巡る基底核連関構築様式 (2018)
  • Author(s): 上園志織、田辺創思、藤原真紀、柘植仁美、中村克樹、井上謙一、高田昌彦
  • Organizer: 第33回 日本大脳基底核研究会
• [Presentation] Manipulation of primate neural networks by means of modified viral vectors (2018)
  • Author(s): Inoue K
  • Organizer: International Conference Viral Manipuration in Monkey
  • Int'l Joint Research / Invited
• [Presentation] 高精細全脳イメージング技術FASTの開発と精神疾患モデルマウスの病態解析 ―脳全体を対象とした仮説フリーな病態・薬物治療機序の組織学的解析― (2018)
  • Author(s): 勢力薫、笠井淳司、丹生光咲、田沼将人、五十嵐久人、中澤敬信、山口瞬、井上謙一、高田昌彦、橋本均
  • Organizer: 第68回日本薬学会近畿支部総会・大会
• [Presentation] Monkey Features Location Identfication Using Convolutional Neural Networks (2018)
  • Author(s): Labuguen R; Gaurav V; Blanco SN; Matsumoto J; Inoue K; Shibata T
  • Organizer: 第28回日本神経回路学会・全国大会
• [Presentation] Optogenetic activation of cutaneous and proprioceptive afferent in the rat sciatic nerve. (2018)
  • Author(s): Kubota S; Sidikejiang W; Kudoh M; Inoue K; Umeda T; Takada M; Seki K
  • Organizer: Neuroscience 2018
  • Int'l Joint Research
• [Presentation] Pathway-selective manipulation of neural network in primates. (2018)
  • Author(s): Inoue K
  • Organizer: フライブルグ大学セミナー
  • Invited
• [Presentation] Development of viral vectors for delivering functional molecules into nonhuman primate brains. (2018)
  • Author(s): Inoue K
  • Organizer: Genetic technologies for systems neurosciences in non-human primates
  • Int'l Joint Research / Invited
• [Presentation] Pathway-selective manipulation of primate neural networks by means of modified viral vectors. (2018)
  • Author(s): Inoue K
  • Organizer: ピッツバーグ大学大学セミナー
  • Invited
• [Presentation] サル腹側中脳は一次運動野を介して筋出力を促通する. (2018)
  • Author(s): 鈴木迪諒、井上謙一、中川浩、伊佐正、高田昌彦、西村幸男
  • Organizer: 次世代脳プロジェクト冬のシンポジウム
• [Presentation] マーモセットにおける大脳基底核および小脳から前部・後部帯状皮質への多シナプス性入力様式 (2018)
  • Author(s): 上園志織、田辺創思、藤原真紀、柘植仁美、中村克樹、井上謙一、高田昌彦
  • Organizer: 第7回日本マーモセット研究会大会
• [Presentation] 化学遺伝学とPET イメージングの融合による黒質線条体ドーパミン神経活動制御 (2018)
  • Author(s): 南本敬史、三村喬生、永井裕司、井上謙一、須原哲也、高田昌彦
  • Organizer: 第7回日本マーモセット研究会大会
• [Presentation] 狂犬病ウイルスゲノムの分節化 (2017)
  • Author(s): 日高侑也、小林由紀、伊藤睦代、林昌宏、井上謙一、伊藤琢也
  • Organizer: 第16回狂犬病研究会
• [Presentation] Cells of origin of multisynaptic projections from amygdala to ventral premotor cortex in macaques (2017)
  • Author(s): Ishida H; Inoue K; Hoshi E; Takada M
  • Organizer: Different elements of Primate Neural Networks in the Connectome Era
  • Int'l Joint Research
• [Presentation] Organization of multisynaptic inputs from the basal ganglia to the premotor cortex in macaque monkeys - Retrograde transneuronal dual tracing using rabies viral vectors. (2017)
  • Author(s): Inoue K; Fujiwara M; Uezono S; Tanabe S; Ishida H; Hoshi E; Takada M
  • Organizer: Cold Spring Harbor Asia Conference: Primate Neuroscience
  • Int'l Joint Research
• [Presentation] 霊長類および齧歯類の線条体入力系におけるFuGシュードタイプレンチウイルスベクターの遺伝子導入特性 (2017)
  • Author(s): 田辺 創思、井上 謙一、上園 志織、柘植 仁美、中村 克樹、小林 和人、高田 昌彦
  • Organizer: 第32回 日本大脳基底核研究会
• [Presentation] マーモセットにおける大脳基底核から帯状皮質への多シナプス性入力様式 (2017)
  • Author(s): 上園 志織、田辺 創思、藤原 真紀、柘植 仁美、中村 克樹、井上 謙一、高田 昌彦
  • Organizer: 第32回 日本大脳基底核研究会
• [Presentation] Differences in retrograde gene transfer efficiency and cytotoxicity between lentiviral vectors pseudotyped with FuG-E and FuG-B2 glycoprotein in the striatal input system of rodents and primates. (2017)
  • Author(s): Tanabe S; Uezono S; Tsuge H; Fujiwara M; Nagaya K; Nagaya N; Sugawara M; Miwa M; Konoike N; Kato S; Nakamura K; Kobayasi K; Inoue K; Takada M
  • Organizer: 第40回日本神経科学大会
• [Presentation] Phase-amplitude coupling in cerebro-basal ganglia-cerebellar networks: A new model of hypo- and hyperkinetic movement disorders. (2017)
  • Author(s): Ninomiya T; Nagai Y; Inoue K; Hori Y; Kikuchi E; Lee J; Suhara T; Iriki A; Minamimoto T; Takada M; Isoda M; Matsumoto M; Mccairn K.W.
  • Organizer: 第40回日本神経科学大会
• [Presentation] Organization of multisynaptic inputs from the basal ganglia to the dorsal and ventral premotor cortices in macaque monkeys: retrograde transneuronal dual tracing with fluorescent rabies viral vectors. (2017)
  • Author(s): Inoue K; Fujiwara M; Uezono S; Tanabe S; Tsuge H; Nagaya N; Nagaya K; Ishida H; Hoshi E; Takada M
  • Organizer: 第40回日本神経科学大会
• [Presentation] The lentiviral vector pseudotyped with FuG-E glycoprotein is more suitable; compared with the FuG-B2 type; for retrograde gene transfer into the cortical input system of primates. (2017)
  • Author(s): Tsuge H; Uezono S; Tanabe S; Fujiwara M; Nagaya K; Nagaya N; Sugawara M; Miwa M; Konoike N; Kato S; Nakamura K; Kobayashi K; Inoue K; Takada M
  • Organizer: 第40回日本神経科学大会
• [Presentation] Organization of multisynaptic inputs from the basal ganglia and cerebellum to the cingulate cortex in common marmosets as revealed by retrograde transneuronal labeling with rabies virus. (2017)
  • Author(s): Uezono S; Tsuge H; Tanabe S; Fujiwara M; Nagaya N; Nagaya K; Inoue K; Takada M
  • Organizer: 第40回日本神経科学大会
• [Presentation] Pathway-selective manipulation of neural circuits (2017)
  • Author(s): Inoue K
  • Organizer: NIMH Workshop Neural Circuits: Gaps and Opportunities
  • Int'l Joint Research / Invited
• [Presentation] 狂犬病ウイルスゲノムの分節化 (2017)
  • Author(s): 日高 侑也、林 昌宏、伊藤 睦代、朴 天鍋、君付 和範、井上 謙一、小林 由紀、伊藤 琢也
  • Organizer: 第160回日本獣医学会学術集会
• [Presentation] Analysis of epitope on rabies virus phosphoprotein recognized by a single chain variable fragment which inhibits rabies virus growth. (2017)
  • Author(s): Kaku Y; Inoue K; Inoue S; Noguchi A; Mirikawa S
  • Organizer: 第65回日本ウイルス学会学術集会
• [Presentation] Pathway-selective optogenetics for elucidating neural network function in primates. (2017)
  • Author(s): Inoue K
  • Organizer: Neuroscience 2017
  • Int'l Joint Research / Invited
• [Presentation] Arrangement of multisynaptic inputs from the basal ganglia to the dorsal and ventral premotor cortical areas in macaques: retrograde transneuronal double labeling with fluorescent rabies viral vectors. (2017)
  • Author(s): Inoue K; Fujiwara M; Uezono S; Tanabe S; Ishida H; Hoshi E; Takada M
  • Organizer: Neuroscience 2017
  • Int'l Joint Research
• [Presentation] FAST; High-speed serial-sectioning imaging for whole brain analysis with high scalability. (2017)
  • Author(s): Kasai A; Seiriki K; Hashimoto T; Niu M; Yamaguchi S; Naka Y; Igarashi H; Tamura M; Nakazawa T; Inoue K; Takada M; Fujita K; Hashimoto H
  • Organizer: Neuroscience 2017
  • Int'l Joint Research
• [Presentation] Organization of multisynaptic inputs from the basal ganglia and cerebellum to the anterior and posterior cingulate cortical areas in common marmosets: Retrograde transneuronal double labeling with fluorescent rabies viral vectors. (2017)
  • Author(s): Uezono S; Tanabe S; Fujiwara M; Tsuge H; Nakamura K; Inoue K; Takada M
  • Organizer: Neuroscience 2017
  • Int'l Joint Research
• [Presentation] Optogenetic modulation of saccade-controlling circuits in the monkey basal ganglia (2017)
  • Author(s): Amita H; Kim H.F; Inoue K; Takada M; Hikosaka O
  • Organizer: Neuroscience 2017
  • Int'l Joint Research
• [Presentation] Differences in retrograde gene transfer efficiency and cytotoxicity between lentiviral vectors pseudotyped with FuG-E and FuG-B2 glycoprotein in rodent and primate brains. (2017)
  • Author(s): Tanabe S; Tsuge H; Uezono S; Fujiwara M; Miwa M; Konoike N; Kato S; Nakamura K; Kobayashi K; Inoue K; Takada M
  • Organizer: Neuroscience 2017
  • Int'l Joint Research
• [Presentation] Cells of origin of multisynaptic projections from amygdala to ventral premotor cortex in macaques (2017)
  • Author(s): Ishida H; Inoue K; Hoshi E; Takada M
  • Organizer: 平成29年度生理研研究会 第7回社会神経科学研究会
• [Presentation] 霊長類における光遺伝学を利用した神経回路操作 (2017)
  • Author(s): 井上 謙一
  • Organizer: 日本学術振興会 光電相互変換第125委員会 第239回研究会 「光技術が切り開く新しいバイオサイエンス・テクノロジー」
  • Invited
• [Presentation] ウイルスベクターを利用した霊長類における神経ネットワーク操作 (2017)
  • Author(s): 井上謙一
  • Organizer: 第6回 生理研-霊長研-脳研 合同シンポジウム
  • Place of Presentation: 新潟大学脳研究所統合脳機能研究センター, 新潟
  • Invited
• [Presentation] Prevention of MPTP-induced parkinsonism by recruitment of calbindin into nigral dopamine neurons. (2017)
  • Author(s): Inoue K, Miyachi S, Nishi K, Okado H, Nagai Y, Minamimoto T, Nambu A, Takada M
  • Organizer: the 12th International Basal Ganglia Society Meeting; IBAGS 2017.
  • Place of Presentation: Merida, Mexico
  • Int'l Joint Research
• [Presentation] 狂犬病ウイルスベクターを用いた逆行性越シナプス的ラベル法によるマーモセット帯状皮質への入力様式の解明：大脳基底核からの入力について (2017)
  • Author(s): 上園志織、柘植仁美、田辺創思、藤原真紀、長屋七奈、長屋清美、井上謙一、高田昌彦
  • Organizer: 第112回日本解剖学会総会・全国学術集会
  • Place of Presentation: 長崎大学、長崎
• [Presentation] Manipulation of primate neural networks by means of modified viral vectors (2016)
  • Author(s): Inoue K
  • Organizer: NHP Chemogenetics Workshop
  • Place of Presentation: National Institute of Health, Bethesda, USA
  • Year and Date: 2016-12-01
  • Int'l Joint Research / Invited
• [Presentation] Primate models for elucidating the circuit pathology of nigrostriatal dopamine system (2016)
  • Author(s): Inoue K
  • Organizer: 第39回日本神経科学大会サテライトシンポジウム：大脳基底核の機能と疾患：基礎と臨床
  • Place of Presentation: パシフィコ横浜，横浜
  • Int'l Joint Research / Invited
• [Presentation] Motivation center in the ventral midbrain directly activates the descending motor pathways via the primary motor cortex. (2016)
  • Author(s): Suzuki M, Inoue K, Nakagawa H, Takada M, Isa T, Nishimura Y
  • Organizer: 第39回日本神経科学大会
  • Place of Presentation: パシフィコ横浜，横浜
• [Presentation] Origin of multisynaptic projections from the amygdala to the forelimb region of the ventral premotor cortex in macaque monkeys. (2016)
  • Author(s): Ishida M, Inoue K, Takada M, Hoshi E
  • Organizer: 第39回日本神経科学大会
  • Place of Presentation: パシフィコ横浜，横浜
• [Presentation] Investigations of spontaneously naturally emerging parkinsonism-cerebellar syndromein an aged Japanese macaque (Macaca fuscata yakui): a potential analogue of multiplesystem atrophy. (2016)
  • Author(s): K.W. McCairn, Ninomiya T, Nagai Y, Go Y, Inoue K, Kimura K, Matsumoto M, Minamimoto M, Isoda M, Takada M
  • Organizer: 第39回日本神経科学大会
  • Place of Presentation: パシフィコ横浜，横浜
• [Presentation] Comparison of efficiency of retrograde gene transfer between lentiviral vectors pseudotyped with FuG-E and FuG-B2 glycoprotein in primate brains: Cortical input system. (2016)
  • Author(s): Tsuge H, Uezono S, Tanabe S, Fujiwara M, Nagaya K, Sugawara M, Miwa M, Konoike N, Kato S, Nakamura K, Kobayashi K, Inoue K, Takada M
  • Organizer: 第39回日本神経科学大会
  • Place of Presentation: パシフィコ横浜，横浜
• [Presentation] Comparison of the efficiency of retrograde gene transfer between lentiviral vectors pseudotyped with FuG-E and FuG-B2 glycoprotein in primate brains: Striatal input system. (2016)
  • Author(s): Tanabe S, Uezono S, Tsuge H, Fujiwara M, Nagaya K, Sugawara M, Miwa M, Konoike N, Kato S, Nakamura K, Kobayashi K, Inoue K,
  • Organizer: 第39回日本神経科学大会
  • Place of Presentation: パシフィコ横浜，横浜
• [Presentation] Use of an optimized chimeric envelope glycoprotein for enhancement of the efficiency of retrograde gene transfer of a pseudotyped lentiviral vector in the primate brain. (2016)
  • Author(s): Inoue K, Tanabe S, Tsuge H, Ueno T, Nagaya K, Fujiwara M, Sugawara M, Kato S, Kobayashi K, Takada M
  • Organizer: 第39回日本神経科学大会
  • Place of Presentation: パシフィコ横浜，横浜
• [Presentation] In vivo electrophysiological evaluation of chanelrhodopsin-2-expressed dorsal root ganglion neurons in adults rats. (2016)
  • Author(s): Seki K, S Wupuer, Umeda T, Inoue K, Kudo M, Takada M
  • Organizer: 第39回日本神経科学大会
  • Place of Presentation: パシフィコ横浜，横浜
• [Presentation] Differential adeno-associated virus mediated gene transfer to dorsal root ganglion neurons with different size in common marmosets. (2016)
  • Author(s): Kudo M, S Wupuer, Inoue K, Takada M, Seki K
  • Organizer: 第39回日本神経科学大会
  • Place of Presentation: パシフィコ横浜，横浜
• [Presentation] 霊長類におけるウイルスベクターを利用した神経回路の光操作 (2016)
  • Author(s): 井上謙一
  • Organizer: ルミノジェネティクス研究会
  • Place of Presentation: KKRホテル熱海,熱海
• [Presentation] Multisynaptic projections from the basal nucleus of the amygdala to the ventral premotor cortex in macaque monkeys. (2016)
  • Author(s): Ishida H, Inoue K, Takada M, Hoshi E
  • Organizer: Neuroscience 2016
  • Place of Presentation: San Diego、USA
  • Int'l Joint Research
• [Presentation] Use of an optimized chimeric envelope glycoprotein enhances the efficiency of retrograde gene transfer of a pseudotyped lentiviral vector in the primate brain. (2016)
  • Author(s): Fujiwara M, Tanabe S, Tsuge H, Uezono S, Nagaya K, Sugawara M, Kato S, Kobayashi K, *Inoue K, Takada M
  • Organizer: Neuroscience 2016
  • Place of Presentation: San Diego、USA
  • Int'l Joint Research
• [Presentation] Studying the structure and function of primate brain by using viral vectors (2016)
  • Author(s): Inoue K
  • Organizer: The 7th International Neural Microcircuit Conference
  • Place of Presentation: 自然科学研究機構 岡崎コンファレンスセンター, 岡崎
  • Int'l Joint Research / Invited
• [Presentation] The lentiviral vector pseudotyped with FuG-E glycoprotien is more suitable, compared with FuG-B2, for retrograde gene transfer in the cortical input system of primate brains. (2016)
  • Author(s): Tsuge H, Uezono S, Tanabe S, Fujiwara M, Sugawara M, Miwa M, Konoike N, Kato S, Nakamura K, Kobayashi K, Inoue K, Takada M
  • Organizer: 第6回マーモセット研究会
  • Place of Presentation: 東京大学, 東京
• [Presentation] Differences in efficiency of retrograde gene transfer and cytotoxity between lentiviral vectors pseudotyped with FuG-E and FuG-B2 glycoprotein in primate brains. (2016)
  • Author(s): Sugawara M, Tanabe S, Uezono S, Tsuge H, Fujiwara M, Miwa M, Konoike N, Kato S, Nakamura K, Inoue K, Takada M, Kobayashi K
  • Organizer: 第6回マーモセット研究会
  • Place of Presentation: 東京大学, 東京
• [Book] Gene Therapy for Neurological Disorders: Methods and Protocols, Methods in Molecular Biology, vol 1382 (Manfredsson FP, ed) (2016)
  • Author(s): Kobayashi K, Kato S, Inoue K, Takada M, Kobayashi K
  • Total Pages: 493
  • Publisher: Springer
• [Remarks] 統合脳システム分野ホームページ
  • URL: http://www.pri.kyoto-u.ac.jp/sections/systems_neuroscience/index.html
• [Remarks] 京都大学霊長類研究所 統合脳システム分野 ホームページ
  • URL: http://www.pri.kyoto-u.ac.jp/sections/systems_neuroscience/index.html