Chromatin dynamics underlying cellular memory
| Project/Area Number |
17002018
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| Research Category |
Grant-in-Aid for Specially Promoted Research
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| Allocation Type | Single-year Grants |
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| Review Section |
Biological Sciences
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| Research Institution | National Institute of Genetics |
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Principal Investigator |
HIROSE Susumu National Institute of Genetics, Department Developmental Genetics, Professor (90022730)
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| Co-Investigator(Kenkyū-buntansha) |
NISHIOKA Kenichi National Institute of Genetics, Department Developmental Genetics, Assistant Professor (80370120)
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| Project Period (FY) |
2005 – 2007
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| Project Status |
Completed (Fiscal Year 2007)
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| Budget Amount *help |
¥225,810,000 (Direct Cost: ¥173,700,000、Indirect Cost: ¥52,110,000)
Fiscal Year 2007: ¥66,170,000 (Direct Cost: ¥50,900,000、Indirect Cost: ¥15,270,000)
Fiscal Year 2006: ¥69,810,000 (Direct Cost: ¥53,700,000、Indirect Cost: ¥16,110,000)
Fiscal Year 2005: ¥89,830,000 (Direct Cost: ¥69,100,000、Indirect Cost: ¥20,730,000)
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| Keywords | Cellular memory / Chromatin / Drosophila / Mouse / GAGA factor / FACT / Supercoling factor / non-coding RNA |
|---|
| Research Abstract |
We have obtained following results through this study.
1. GAGA factor FACT complex recruits chromatin remodeling complex PBAP to Pc/trx response elements, induces chromatin remodeling, and maintains expression of Hox genes.
2. GAGA factor-FACT complex binds to a GA repeat sequence downstream the white gene, induces chromatin remodeling, replaces histone H3 by its variant H3.3, and maintains white gene expression by counteracting the heterochromatin spreading.
3. Transcription of non-coding RNA through Pc/trx response element regulates Hox gene expression.
4. Supercoiling factor is recruited to chromatin acetylated at histone H4 K16 by MOF, and contributes dosage compensation of X chromosome through counteracting with ISWI.
5. Ash1 knock-out mice are homozygous viable but show homeotic transformation of the back-one. SPT16 knock-out mice are embryonic lethal in homozygotes. Its heterozygotes show a deviation in the levels of IgG sublasses.
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Report
(7 results)
Research Products
(145 results)
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[Journal Article] Drosophila Blimp-1 is a transient transcriptional repressor that controls timing of the ecdysone-induced developmental pathyway.2007
Author(s)
Agawa, Y., Sarhan, M., Kageyama, Y., Akagi, K., Takai, M., Hayashiyama, K., Wada, T., Handa, H., Iwamatsu, A., Hirose, S., Ueda, H.
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Journal Title
Mol. Cell. Biol. 27
Pages: 8739-8747
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Linker histone variants control chromatin dynamics during early embryogenesis.2005
Author(s)
Saeki, H., Ohsumi, K., Aihara, H., Ito, T., Hirose, S., Ura, K., Kaneda, Y.
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Journal Title
Proc. Natl. Acad. Sci. USA 102
Pages: 5697-5702
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Solution Structure of the HMG-box domain in the SSRP1 subunit of FACT.2005
Author(s)
Kasai, N., Tsunaka, Y., Ohki, I., Hirose, S., Morikawa, K., Tate, S.
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Journal Title
J. Biomol. NMR 32
Pages: 83-88
Description
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[Presentation] 遺伝子発現と発生分化2007
Author(s)
広瀬 進
Organizer
国立遺伝学研究所シンポジウム
Place of Presentation
三島
Year and Date
2007-04-20
Description
「研究成果報告書概要(和文)」より
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[Presentation] Transcriptional elongation of non-coding RNAs promoted by the Trithorax TAC1 complex represses Ubx by a transcriptional repression mechanism.2007
Author(s)
Petruck, S., Sedkov, Y., Riley, K., Hodgson, J., Schweisguth, R, Hirose, S., Janes, J., Brock, H., Mazo, A.
Organizer
47^<th> Annual Drosophila Research Conference
Place of Presentation
Philadelphia
Description
「研究成果報告書概要(欧文)」より
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[Presentation] 細胞記憶のメカニズム2006
Author(s)
広瀬 進
Organizer
半田研究室15周年記念シンポジウム
Place of Presentation
東京
Year and Date
2006-11-08
Description
「研究成果報告書概要(和文)」より
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[Presentation] 170 is a labile transcription factor induced by ecdysone in Drosophila melanogaster.2006
Author(s)
Sarhan, M., Takai, M., Agawa, Y., Hashiyama, K., Hirose, S., Ueda, H.
Organizer
20^<th> IUBMB International Congress of Biochmistry and Molecular Biology and 11^<th> OBMB Congress
Place of Presentation
Kyoto
Description
「研究成果報告書概要(欧文)」より
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[Presentation] Structural Analysis of human nucleosome core particles.2006
Author(s)
Tsunaka, Y., Tate, S., Okuwaki, M., Nagata, K., Hirose, S., Morikawa, K.
Organizer
20^<th> IUBMB International Congress of Biochmistry and Molecular Biology and II^<th> OBMB Congress
Place of Presentation
Kyoto
Description
「研究成果報告書概要(欧文)」より
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[Presentation] Biological function of the ISWI chromatin remodeling complex RSF.2006
Author(s)
Hanai, K., Nakayama, T., Furuhashi, H., Nishioka, K., Yamamoto, T., Akasaka, K., Hirose, S.
Organizer
20^<th> IUBMB International Congress of Biochmistry and Molecular Biology and 11^<th> OBMB Congress
Place of Presentation
Kyoto
Description
「研究成果報告書概要(欧文)」より
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[Presentation] Biological function of chromatin remodeling factor RSF.2005
Author(s)
Hanai, K., Nakayama, T., Furuhashi, H., Nishioka, K., Yamamoto, T., Akasaka, K., Hirose, S.
Organizer
28^<th> Japan Molecular Biology Society Meeting
Place of Presentation
Fukuoka
Description
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