Reverse Genetic Analysis of DNA Damage responses in the Avian DT40 Cell Line and the Medaka Fish

• Project/Area Number: 17013039
• Research Category: Grant-in-Aid for Scientific Research on Priority Areas
• Allocation Type: Single-year Grants
• Review Section: Biological Sciences
• Research Institution: Kyoto University
• Principal Investigator: TAKEDA Shunichi Kyoto University, 大学院・医学研究科, 教授 (60188191)
• Co-Investigator(Kenkyū-buntansha): TANIGUCHI Yoshihito 京都大学, 大学院・医学研究科, 准教授 (00324616)
• Project Period (FY): 2005 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥120,000,000 (Direct Cost: ¥120,000,000); Fiscal Year 2009: ¥24,000,000 (Direct Cost: ¥24,000,000); Fiscal Year 2008: ¥24,000,000 (Direct Cost: ¥24,000,000); Fiscal Year 2007: ¥24,000,000 (Direct Cost: ¥24,000,000); Fiscal Year 2006: ¥24,000,000 (Direct Cost: ¥24,000,000); Fiscal Year 2005: ¥24,000,000 (Direct Cost: ¥24,000,000)
• Keywords: 癌 / ゲノム / 細胞・組織 / バイオテクノロジー / 放射線 / DNA組み換え / DNA修復 / 変異 / ゲノム不安定性 / シスプラチン / DT40 / メダカ / p53 / 放射線治療 / トポイソメラーゼ阻害薬 / 相同組み換え

Research Abstract

A number of anti-cancer agents, including radiotherapy, induce DNA damage, arrest DNA replication, and thereby induce apoptosis. Therefore, functional analysis of DNA repair factors may contribute to improvement of anti-cancer treatments. For this functional analysis, we have developed unique reverse genetic model systems, the avian somatic cell line (DT40) and the Medaka fish. We have revealed the role of ubiquitylation, some DNA polymerases, and BRCA1/2 in DNA damage responses.

Research Products

• [Journal Article] accumulation of RAD51 at double-strand (2010) GEMIN2 promotes breaks in homologous recombination. (2010)
  • Author(s): Takizawa Y, Qing Y, Takaku M, Ishida T, Morozumi Y, Tsujita T, Kogame T, Hirota K, Takahashi M, Shibata T, Kurumizaka H, Takeda S.
  • Journal Title: Nucleic Acids Res. (in press)
  • Peer Reviewed
• [Journal Article] DNA polymerasesνandθare Takeda S. required for efficient Immunoglobulin V gene diversification in chicken. (2010)
  • Author(s): Kohzaki M, Nishihara K, Hirota K, Sonoda E, Yoshimura M, Ekino S, Butler JE, Watanabe M, Halazonetis T
  • Journal Title: J C e l l B i o l. (in press)
  • Peer Reviewed
• [Journal Article] Collaborative action of Brca1 Taniguchi and CtIP in elimination of covalent modifications from double-strand breaks to facilitate subsequent break repair. (2010)
  • Author(s): Nakamura K, Kogame T, Oshiumi H, Shinohara A, Sumitomo Y, Agama K, Pommier Y, Tsutsui KM, Tsutsui K, Hartsuiker E, Ogi T, Takeda S, Y.
  • Journal Title: P L o S G e n e t. 6
  • Peer Reviewed
• [Journal Article] PTIP promotes DNA double-strand break repair through homologous recombination. (2010)
  • Author(s): Wang X, Takenaka K, Takeda S.
  • Journal Title: G e n e s C e l l s (in press)
  • Peer Reviewed
• [Journal Article] approach using DNA-repair-deficient (2010) A novel chicken DT40 cell lines for screening and characterizing the genotoxicity of environmental contaminants. (2010)
  • Author(s): Ji K, Kogame T, Choi K, Wang X, Lee J, Taniguchi Y, Takeda S.
  • Journal Title: Environ Health Perspect. 117 Pages: 1737-44
  • Peer Reviewed
• [Journal Article] A novel approach using DNA-repair-deficient chicken DT40 cell lines for screening and characterizing the genotoxicity of environmental contaminants. Environ Health Perspect (2010)
  • Author(s): Ji K, Kogame T, Choi K, Wang X, Lee J, Taniguchi Y, Takeda S.
  • Journal Title: Environ Health Perspect. VOL.117 Pages: 1737-1744
  • Peer Reviewed
• [Journal Article] Collaborative action of Brcal and CtIP in elimination of covalent modifi cations from double-strand breaks to facilitate subsequent break repair. (2010)
  • Author(s): Nakamura K, Kogame T, Oshiumi H, Shinohara A, Sumitomo Y, Agama K, Pommier Y, Tsutsui KM, Tsutsui K, Hartsuiker E, Ogi T, Takeda S, Taniguchi Y.
  • Journal Title: PLoS Genet. VOL.6
  • Peer Reviewed
• [Journal Article] Bloom DNA helicase facilitates Hirota K. (2009) homologous recombination between diverged homologous sequences. (2009)
  • Author(s): Kikuchi K, Abdel-Aziz HI, Yamazoe M, Taniguchi Y, Takeda S
  • Journal Title: J B i o l C h e m. 284 Pages: 26360-7
  • Peer Reviewed
• [Journal Article] the non-homologous end-joining repair Okada N. (2009) Genetic evidence that pathway is involved in LINE retrotransposition. (2009)
  • Author(s): Suzuki J, Yamaguchi K, Kajikawa M, Ichiyanagi K, Adachi N, Koyama H, Takeda S
  • Journal Title: P L o S G e n e t. 5
  • Peer Reviewed
• [Journal Article] An ATM and ATR Takeda S, dependent checkpoint inactivates spindle assembly by targeting CEP63. (2009)
  • Author(s): Smith E, Dejsuphong D, Balestrini A, Hampel M, Lenz C, Vindigni A, Costanzo V.
  • Journal Title: N a t C e l l B i o l. 11 Pages: 278-85
  • Peer Reviewed
• [Journal Article] for single-strand lesions initiating (2009) Genetic evidence Nbs1-dependent homologous recombination in diversification of Ig V in chicken B lymphocytes. (2009)
  • Author(s): Nakahara M, Sonoda E, Nojima K, Sale JE, Takenaka K, Kikuchi K, Nakamura K, Sumitomo Y, Bree RT, Lowndes Taniguchi Y, NF, Takeda S.
  • Journal Title: P L o S G e n e t. 5
  • Peer Reviewed
• [Journal Article] Cohesin associates with Fukagawa T, Yen TJ, Yokomori spindle poles in a mitosis-specific manner and functions in spindle assembly in vertebrate cells. (2009)
  • Author(s): Kong X, Ball AR, Jr., Sonoda E, Feng J, Takeda S, K.
  • Journal Title: M o l B i o l C e l l. 20 Pages: 1289-301
• [Journal Article] Bloom DNA helicase facilitates homologous recombination between diverged homologous sequences. (2009)
  • Author(s): Kikuchi K, Abdel-Aziz HI, Taniguchi Y, Yamazoe M, Takeda S, Hirota K.
  • Journal Title: J Biol Chem. VOL.284 Pages: 26360-26367
  • Peer Reviewed
• [Journal Article] The vital link between the ubiquitin-proteasome pathway and DNA repair : Impact on cancer therapy. (2009)
  • Author(s): Motegi A, Murakawa Y, Takeda S.
  • Journal Title: Cancer Lett. VOL.283 Pages: 1-9
  • Peer Reviewed
• [Journal Article] Cohesin associates with spindle poles in a mitosis-specificmanner and functions in spindle assembly in vertebrate cells (2009)
  • Author(s): Kong X, Ball AR, Jr., Sonoda E, Fang J, Takeda S, Fukagawa T, Yen TJ, Yokoniori K
  • Journal Title: Mol Cell Biol 20 Pages: 1289-1301
  • Peer Reviewed
• [Journal Article] The Vital Link between the Ubiquitin-Proteasome Pathway and DNA Repair : Impact on Cancer Therapy (2009)
  • Author(s): Motegi A, Murakawa Y, Takeda S
  • Journal Title: Cancer Lett (in press)
  • Peer Reviewed
• [Journal Article] Genetic evidence for single-strand lesions initiating Nbs1-dependent homologousrecombination in diversification of Ig V in chicken B lymphocytes (2009)
  • Author(s): Nakahara M, Sonoda E, Nojima K, Sale JE, Takenaka K, Kikuchi K, Taniguchi Y, Nakamura K, Sumitomo Y, Bree RT, Lowndes NF, Takeda S
  • Journal Title: PLoS Genet 5
  • Peer Reviewed
• [Journal Article] An ATM and ATR dependent checkpoint inactivates spindle assembly by targeting CEP63 (2009)
  • Author(s): Smith E, Dejsuphong D, Balestrini A, Hampel M, Lenz C, Vindigni A, Takeda S, Costanzo V
  • Journal Title: Nat Cell Biol 11 Pages: 278-285
  • Peer Reviewed
• [Journal Article] Genetic Evidence That the Non-homologous End-joining Repair Pathway Is Involved in LINE Retrotransposition (2009)
  • Author(s): Suzuki J, Yamaguchi K, Kajikawa M, Ichiyanagi K, Adachi N, Koyama H, Takeda S, Okada N
  • Journal Title: PLoS Genet
  • Peer Reviewed
• [Journal Article] Cyclin-dependent kinases and cell-cycle Hunt T. transitions: does one fit all? (2008)
  • Author(s): Hochegger H, Takeda S
  • Journal Title: Nat Rev M o l C e l l B i o l 9(Review) Pages: 910-6
  • Peer Reviewed
• [Journal Article] PARP-1 ensures the modulation of replication Okumura K. fork progression by homologous recombination on damaged DNA. (2008)
  • Author(s): Sugimura K, Takebayashi S, Taguchi H, Takeda S
  • Journal Title: J C e l l B i o l. 183 Pages: 1203-12
  • Peer Reviewed
• [Journal Article] The 9-1-1 DNA clamp is Takeda S, required for immunoglobulin gene conversion. (2008)
  • Author(s): Saberi A, Nakahara M, Sale JE, Kikuchi K, Arakawa H, Buerstedde J-M, Sonoda E.
  • Journal Title: M o l C e l l B i o l. 28 Pages: 6113-22
  • Peer Reviewed
• [Journal Article] Single stranded DNA binding protein hSSB1 is critical for genomic stability. (2008)
  • Author(s): Richard D, Bolderson E, Cubeddu L, Wadsworth RIM, Savage K, Sharma GG, Nicolette ML, Tsvetanov S, McIlwraith MJ, Pandita R, Takeda S, West SC, Paull TT, Pandita1 TK, White MF, Hay TT, Gautier J, Khanna KK.
  • Journal Title: N a t u r e 453 Pages: 677-81
  • Peer Reviewed
• [Journal Article] DNA damage response protein ASCIZ links base excision repair with immunoglobulin gene conversion. (2008)
  • Author(s): Oka H, Sakai W, Sonoda E, Nakamura J, Asagoshi K, Wilson SH, Kobayashi M, Yamamoto K, Heierhorst J, Takeda S, Taniguchi Y.
  • Journal Title: B i o c h e m B i o p h y s R e s C o m m u n. 371 Pages: 225-9
  • Peer Reviewed
• [Journal Article] Ku70 prevents genome instability resulting from heterozygosity of the telomerase RNA component in a vertebrate tumour line (2008)
  • Author(s): Faure V, Wenner T, Cooley C, Bourke E, Farr CJ, Takeda S, Morrison CG
  • Journal Title: DNA Repair (Mist) 7 Pages: 713-724
  • Peer Reviewed
• [Journal Article] DNA damage response protein ASCIZ links base excision repair with immunoglobulin gene conversion (2008)
  • Author(s): Oka H. Sakai if, Sonoda E, Nakamura J, Asagoshi K, Wilson Si, Kobayashi N, Yamamoto K. Heierhorst J, Takeda S. Taniguchi Y
  • Journal Title: Biochem Biophys Rei Commun 371 Pages: 225-229
  • Peer Reviewed
• [Journal Article] Single stranded DNA binding protein hSSB1 is critical for genomic stability (2008)
  • Author(s): Richard K, Boidersan E. Cubeddu L, Wadsonrth RIM, Savage K. Sharma CG, Nicolette ML, Tsvetanov S. MIlwraith MJ. Pandita R, Takeda S. Hay TT. Gautier J. West SC. Paull TT. Pandital TR. White MF. Khanna KK
  • Journal Title: Nature 453 Pages: 677-681
  • Peer Reviewed
• [Journal Article] The 9-1-1 DNA clamp is required for immunoglobulin gene conversion (2008)
  • Author(s): Saberi A, Nakahara M, Sale JE, Kikuchi K, Arakawa H, Buerstedde J-M, Takeda S, Sonoda E
  • Journal Title: Mol Cell Biol 28 Pages: 6113-6122
  • Peer Reviewed
• [Journal Article] KU70/80, DNA-PKcs, and Artemis are essential for the rapid induction of apoptosis after massive DSB formation (2008)
  • Author(s): Abe T, Ishiai H. Hosono Y, Yoshimura A, Tada S, Adachi N. Koyama H. Takata N, Takeda S. Enomoto T. Seki M
  • Journal Title: Cell Signal 20 Pages: 1978-1985
  • Peer Reviewed
• [Journal Article] PARP-1 ensures the modulation of replication fork progression by homologous recombination on damaged DNA (2008)
  • Author(s): Sugimura K, Takebayashi S, Taguchi H, Takeda S, Okumura K
  • Journal Title: J Cell Biol 183 Pages: 1203-1212
  • Peer Reviewed
• [Journal Article] Cyclin-dependent kinases and cell-cycle transitions : does onefit all? (2008)
  • Author(s): Hochegger H, Takeda S, Hunt T
  • Journal Title: Nat Rev Mol Cell Biol (Review) 9 Pages: 910-916
  • Peer Reviewed
• [Journal Article] Single stranded DNA binding protein hSSB1 is critical for genomic stability. (2008)
  • Author(s): Richard D, Bolderson E, Cubeddu L, Wadsworth RIM, Savage K, Sharma GG, Nicolette M.L, Tsvetanov S, Mcllwraith MJ, Pandita R, Takeda S, Hay TT, Gautier J, West SC, Paull TT, Pandital TK, White MF, Khanna KK
  • Journal Title: Nature (in press)
  • Peer Reviewed
• [Journal Article] Ctp1/CtIP and the MRN Taniguchi Y, complex collaborate in the initial steps of homologous recombination. (2007)
  • Author(s): Takeda S, Nakamura K, Paull TT.
  • Journal Title: M o l C e l l. 28(Review) Pages: 351-2
  • Peer Reviewed
• [Journal Article] Connecting the Dots between Septins and the DNA Damage Checkpoint. (2007)
  • Author(s): Kinoshita M, Takeda S.
  • Journal Title: C e l l 130(Review) Pages: 777-9
  • Peer Reviewed
• [Journal Article] RAD51 up-regulation bypasses BRCA1 function Bishop DK. and is a common feature of BRCA1-deficient breast tumors. (2007)
  • Author(s): Martin RW, Orelli BJ, Yamazoe M, Minn AJ, Takeda S
  • Journal Title: C a n c e r R e s. 67 Pages: 9658-65
  • Peer Reviewed
• [Journal Article] Functional interactions between BLM and XRCC3 in the cell. (2007)
  • Author(s): Otsuki M, Seki M, Inoue E, Yoshimura A, Kato G, Yamanouchi S, Kawabe Y, Tada S, Shinohara A, Komura J, Ono T, Takeda S, Ishii Y, Enomoto T.
  • Journal Title: J C e l l B i o l. 179 Pages: 53-63
• [Journal Article] Inhibitors of the proteasome suppress homologous DNA recombination in mammalian cells. (2007)
  • Author(s): Murakawa Y, Sonoda E, Barber LJ, Zeng W, Yokomori K, Kimura H, Niimi A, Lehmann AR, Zhao GY, Hochegger H, Boulton SJ, Takeda S.
  • Journal Title: C a n c e r R e s. 67 Pages: 8536-8543
  • Peer Reviewed
• [Journal Article] An essential role for Cdk1 in S phase control, revealed by chemical genetics in vertebrate cells. (2007)
  • Author(s): Hochegger H, Dejsuphong D, Sonoda E, Saberi A, Rajendra E, Kirk J, Hunt T, Takeda S.
  • Journal Title: J C e l l B i o l. 178 Pages: 257-68
  • Peer Reviewed
• [Journal Article] Poly (ADP-ribose) Takeda S, Polymerase-1 Accelerates Single-Strand Break Repair in Concert with Poly (ADP-ribose) Glycohydrolase. (2007)
  • Author(s): Fisher A, Hochegger H, Caldecott KW.
  • Journal Title: M o l C e l l B i o l. 27 Pages: 5597-5605
  • Peer Reviewed
• [Journal Article] RAD18and poly[ADPribose]polymerase independently suppress the access of nonhomologous end joining to double strand breaks and facilitate homologous recombination mediated repair. (2007)
  • Author(s): Saberi A, Hochegger H, Szuts D, Lan L, Yasui A, Sale JE, Taniguchi Y, Y, Hua W, Yokomori K, Helleday T, Teraoka Murakawa H, Arakawa H, Buerstedde JM, Takeda S.
  • Journal Title: M o l C e l l B i o l. 27 Pages: 2562-2571
  • Peer Reviewed
• [Journal Article] Cooperative Roles of Vertebrate Fbh1 and Blm DNA Helicases in Avoidance of Crossovers during Recombination Initiated by Replication Fork Collapse. (2007)
  • Author(s): Kohzaki M, Hatanaka A, Sonoda E, Yamazoe M, Kikuchi K, Trung NV, Szuts D, Sale JE, Shinagawa H, Watanabe M, Takeda S.
  • Journal Title: M o l C e l l B i o l. 27 Pages: 2812-2820
  • Peer Reviewed
• [Journal Article] A critical role for the ubiquitin-conjugating enzyme Ubc13 in initiating homologous recombination. (2007)
  • Author(s): Zhao GY, Sonoda E, Barber LJ, Oka H, Murakawa Y, Yamada K, Ikura T, Wang X, Kobayashi M, Yamamoto K, Boulton SJ, Takeda S.
  • Journal Title: M o l C e l l. 25 Pages: 663-675
  • NAID: 130007000910
  • Peer Reviewed
• [Journal Article] DNA-Dependent Protein Kinase Inhibits AID-Induced Antibody Gene Conversion. (2007)
  • Author(s): Cook AJ, Raftery JM, Lau KK, Jessup A, Harris RS, Takeda S, Jolly CJ
  • Journal Title: PLoS Biol. 5 Pages: 80-80
  • Peer Reviewed
• [Journal Article] Cooperative Roles of Vertebrate Fbhl and Blm DNA Helicases in Avoidance of Crossovers during Recombination Initiated by Replication Fork Collapse. (2007)
  • Author(s): Kohzaki M, llatanaka A, Sonoda E, Yamazoe M, Kikuchi K, Trung NV, Szuts D, Sale JE, Shinagawa H, Watanabe M, Takeda S
  • Journal Title: Mol Cell Biol. 27 Pages: 2812-2820
  • Peer Reviewed
• [Journal Article] RAD18 and poly [ADP ribose]polymerase independently suppress the access of nonhomologous end joining to double strand breaks and facilitate homologous recombination mediated repair. (2007)
  • Author(s): Saberi A, Hochegger H, Szuts D, Lan L, Yasui A, Sale JE, Taniguchi Y, Murakawa Y, Hua W, Yokomori K, Helleday T, Teraoka H, Atakawa H, Buerstedde JM, Takeds S
  • Journal Title: Mol Cell Biol. 27 Pages: 2562-2571
  • Peer Reviewed
• [Journal Article] Interplay between DNA polymerases beta and lambda in repair of oxidation DNA damage in chicken DT40 cells. (2007)
  • Author(s): Tano K, Nakamura J, Asagoshi K, Arakawa H, Sonoda E, Braithwaite EK, Prasad R, Buerstedde JM, Takeda S, Watanabe M, Wilson SH
  • Journal Title: DNA Repair (Amst) 6 Pages: 869-875
  • Peer Reviewed
• [Journal Article] Histone H1 variant, H1R is involved in DNA damage response. (2007)
  • Author(s): Hashimoto H, Sonoda E, Takami Y, Kimura H, Nakayama T, Tachibana M, Takeda S, Shinkai Y
  • Journal Title: DNA Repair 6 Pages: 1584-1594
  • Peer Reviewed
• [Journal Article] Poly (ADP-ribose) Polymerase-1 Accelerates Single-Strand Break Repair in Concert with Poly (ADP-ribose) Glycohydrolase. (2007)
  • Author(s): Fisher A, Hochegger H, Takeda S, Caldecott KW
  • Journal Title: Mol Cell Biol. 27 Pages: 5597-5605
  • Peer Reviewed
• [Journal Article] An essential role for Cdkl in S phase control, revealed by chemical genetics in vertebrate cells. (2007)
  • Author(s): Hochegger H, Dejsuphong D, Sonoda E, Saberi A, Rajendra E, Kirk J, Hunt T, Takeda S
  • Journal Title: J Cell Biol. 178 Pages: 257-268
  • Peer Reviewed
• [Journal Article] Inhibitors of the proteasome suppress homologous DNA recombination in mammalian cells. (2007)
  • Author(s): Murakawa Y, Sonoda E, Barber LJ, Zeng W, Yokomori K, Kimura H, Niimi A, Lehmann AR, Zhao GY, Hochegger H, Boulton SJ and Takeda S
  • Journal Title: Cancer Res. 67 Pages: 8536-8543
  • Peer Reviewed
• [Journal Article] DNA damage-dependent acetylation and ubiquitination 1 of H2AX enhances chromatin dynamics. (2007)
  • Author(s): Ikura T, Tashiro S, Kakino A, Shima H, Jacob N, Amunugama R, Yoder K, Izumi S, Kuraoka I, Tanaka K, Kimura H, Ikura M, Nishikubo S, Ito T, Muto A, Miyagawa K, Takeda S, Fishel R, Igarashi K and Kamiya K
  • Journal Title: Mol Cell Biol. 27 Pages: 7028-7040
  • Peer Reviewed
• [Journal Article] Functional interactions between BLM and XRCC3 in the cell. (2007)
  • Author(s): Otsuki M, Seki M, Inoue E, Yoshimura A, Koto G, Yamanouchi S, Kawabe Y, Tada S, Shinohara A, Komura J, Ono T, Takeda S, Ishii Y, and Enomoto T
  • Journal Title: J Cell Biol. 179 Pages: 53-63
  • Peer Reviewed
• [Journal Article] Cells deficient in the FANC/BRCA pathway are hypersensitive to plasma levels of formaldehyde. (2007)
  • Author(s): Ridpath JR, Nakamura A, Tano K, Luke A.M., Sonoda E, Arakawa E, Buerstedde J-M, Gillespie DAF, Sale JE, Yamazoe M, Bishop DK, Takata M, Takeda S, Watanabe M, Swenberg JA, Nakamura J.
  • Journal Title: Cancer Res. 67 Pages: 11117-11122
  • Peer Reviewed
• [Journal Article] RAD51 up-regulation bypasses BRCA1 function and is a common feature of BRCA1-deficient breast tumors. (2007)
  • Author(s): Martin RW, Orelli BJ, Yamazoe M, Minn AJ, Takeda S, Bishop DK
  • Journal Title: Cancer Res. 67 Pages: 9658-9665
  • Peer Reviewed
• [Journal Article] DDB1 gene disruption causes a severe growth defect and apoptosis in chicken DT40 cells. (2007)
  • Author(s): Wakasugi M, Matsuura K, Nagasawa A, Fua D, Shimizu H, Yamamoto K, Takeda S, Matsunaga T
  • Journal Title: Biochem Biophys Res Commun. 364 Pages: 771-777
  • Peer Reviewed
• [Journal Article] Ctp1/CtIP and the MRN complex collaborate in the initial steps of homologous recombination. (Review) (2007)
  • Author(s): Takeda S, Nakamura K, Taniguchi Y, Paull TT
  • Journal Title: Mol Cell 28 Pages: 351-352
  • Peer Reviewed
• [Journal Article] A critical role for the ubiquitin-conjugating enzyme Ubc13 in initiating homologous recombination. (2007)
  • Author(s): Zhao GY, Sonoda E, Barber LJ, Oka H, Murakawa Y, Yamada K, Ikura T, Wang X, Kobayashi M, Yamamoto K, Boulton SJ, Takeda S
  • Journal Title: Mol Cell Biol 25 Pages: 663-675
  • NAID: 130007000910
• [Journal Article] Generation of medaka gene knockout models by target-selected mutagenesis. (2006)
  • Author(s): Taniguchi Y, Takeda S, M, Kamei Y, Todo T, Sasado T, Deguchi T, Furutani-Seiki Kondoh H, Mudde J, Yamazoe M, Hidaka M, Mitani H, Toyoda A, Sakaki Y, Plasterk RH, Cuppen E.
  • Journal Title: G e n o m e B i o l. 7(12)
  • Peer Reviewed
• [Journal Article] Vertebrate POLQ and POL cooperate in Base Excision Repair ofβOxidative DNA Damage. (2006)
  • Author(s): Yoshimura A, Kohzaki M, Nakamura J, Asagoshi K, Sonoda E, Hou E, Prasad R, Wilson SH, Tano K, Yasui A, Lan L, Seki M, Wood RD, Arakawa H, Buerstedde JM, Hochegger H, Okada T, Hiraoka M, Takeda S.
  • Journal Title: M o l C e l l. 24 Pages: 115-25
  • Peer Reviewed
• [Journal Article] Ubiquitin-Binding Motifs in REV1 Protein Are Required For Its Role in the Tolerance of DNA Damage. (2006)
  • Author(s): Guo C, Tang TS, Bienko M, Parker JL, Bielen AB, Sonoda E, Takeda S, Dikic I, Ulrich HD, Friedberg EC.
  • Journal Title: M o l C e l l B i o l. 26 Pages: 8892-900
  • Peer Reviewed
• [Journal Article] end-joining and homologous Differential usage of non-homologous recombination in double strand break repair. (2006)
  • Author(s): Sonoda E, Hochegger H, Saberi A, Taniguchi Y, Takeda S.
  • Journal Title: D N A R e p a i r ( A m s t ) 5(Review) Pages: 1021-9
  • Peer Reviewed
• [Journal Article] Interacts with PCNA: Significance of the REV1 BRCT Domain In Vitro and In REV1 Protein Vivo. (2006)
  • Author(s): Guo C, Sonoda E, Tang TS, Parker JL, Bielen AB, Takeda S, Ulrich HD, Friedberg EC.
  • Journal Title: M o l C e l l. 23 Pages: 265-71
  • Peer Reviewed
• [Journal Article] Genetic dissection of vertebrate 53BP1: a major role in non-homologous end joining of DNA double strand breaks. (2006)
  • Author(s): akamura K, Sakai W, Kawamoto T, Bree RT, Lowndes NF, Takeda S, Taniguchi Y.
  • Journal Title: D N A R e p a i r ( A m s t ) 5 Pages: 741-749
  • Peer Reviewed
• [Journal Article] Critical roles for Polζin Cellular Tolerance to Yamazoe M. Nitric Oxide induced DNA Damage. (2006)
  • Author(s): Xiaohua W, Takenaka K, Sonoda E, Hochegger H, Kawanishi S, Kawamoto T, Takeda S
  • Journal Title: C a n c e r R e s. 66 Pages: 748-54
  • Peer Reviewed
• [Journal Article] Genetic dissection of vertebrate 53BP1 : a major role in non-homologous end joining of DNA double strand breaks. (2006)
  • Author(s): Nakamura K, Sakai W, Kawamoto T, Bree RT, Lowndes NF, Takeda S, Taniguchi Y
  • Journal Title: DNA Repair (Amst) 5 Pages: 741-749
• [Journal Article] REV1 Protein Interacts with PCNA : Significance of the REV1 BRCT Domain In Vitro and In Vivo. (2006)
  • Author(s): Guo C, Sonoda E, Tang TS, Parker JL, Bielen AB, Takeda S, Ulrich HD, Friedberg EC
  • Journal Title: Mol Cell 23 Pages: 265-271
• [Journal Article] Differential usage of non-homologous end-joining and homologous recombination in double strand break repair. (2006)
  • Author(s): Sonoda E, Hochegger H, Saberi A, Taniguchi Y, Takeda S
  • Journal Title: DNA Repair (Amst) 5 Pages: 1021-1029
• [Journal Article] Ubiquitin-Binding Motifs in REV1 Protein Are Required For Its Role in the Tolerance of DNA Damage. (2006)
  • Author(s): Guo C, Tang TS, Blenko M, Parker JL, Blelen AB, Sonoda E, Takeda S, Ulrich HD, Dikic I, Friedberg EC
  • Journal Title: Mol Cell Biol 26 Pages: 8892-8900
• [Journal Article] Vertebrate POLQ and POLβ cooperate in Base Excision Repair of Oxidative DNA Damage. Mol Cell. 24 : 115-25 (2006)
  • Author(s): Yoshimura A, Kohzaki M, Nakamura J, Asagoshi K, Sonoda E, Hou E, Prasad R, Wilson SH, Tano K, Yasui A, Lan L, Seki M, Wood RD, Arakawa H, Buerstedde JM, Hochegger H, Okada T, Hiraoka M, Takeda S
  • Journal Title: Mol Cell 24 Pages: 115-125
• [Journal Article] Generation of medaka gene knockout models by target-selected mutagenesis. (2006)
  • Author(s): Taniguchi Y, Takeda S, Furutani-Seiki M, Kamei Y, Todo T, Sasado T, Deguchi T, Kondoh H, Mudde J, Yamazoe M, Hidaka M, Mitani H, Toyoda A, Sakaki Y., Plasterk RH., Cuppen E
  • Journal Title: Genome Biol 7
• [Journal Article] Critical roles for Polζ in Cellular Tolerance to Nitric Oxide induced DNA Damage (2006)
  • Author(s): Wu X, Takenaka K, Sonoda E, Hochegger H, Kawanishi S, Kawamoto T, Takeda S, Yamazoe
  • Journal Title: Cancer Research 66 Pages: 748-754
• [Journal Article] Multiple repair pathways mediate tolerance to chemotherapeutic cross-linking agents in vertebrate cells. (2005)
  • Author(s): Nojima K, Hochegger H, Saberi A, Fukushima T, Kikuchi K, Yoshimura M, Orelli BJ, Bishop DK, Hirano S, Ohzeki M, Ishiai M, Yamamoto K, Takata M, Arakawa H, Buerstedde JM, Yamazoe M, Kawamoto T, Araki K, Takahashi JA, Hashimoto N, Takeda S, Sonoda E.
  • Journal Title: C a n c e r R e s. 65 Pages: 11704-11
  • Peer Reviewed
• [Journal Article] Rad51 paralog deficiency on Similar effects of Brca2 truncation and immunoglobulin V gene diversification in DT40 cells support an early role for Rad51 paralogs in homologous recombination. (2005)
  • Author(s): Hatanaka A, Yamazoe M, Sale J.E., Takata M, Yamamoto K, Kitao H, Sonoda E, Kikuchi K, Yonetani Y, Takeda S.
  • Journal Title: M o l C e l l B i o l. 25 Pages: 1124-34
  • Peer Reviewed
• [Journal Article] in Homologous DNA Recombination Dual Roles for DNA Polymerase h and Translesion DNA Synthesis. (2005)
  • Author(s): Kawamoto T, Araki K, Sonoda E, Yamashita, Y.M., Harada, K.K., Kikuchi1, K., Masutani, C., Hanaoka, F., Nozaki, K., Hashimoto, N., Takeda, S.
  • Journal Title: M o l C e l l. 20 Pages: 793-9
  • Peer Reviewed
• [Journal Article] Similar effects of Brca2 truncation and Rad51 paralog deficiency on immunoglobulin V gene diversification in DT40 cells support an early role for Rad51 paralogs in homologous recombination. (2005)
  • Author(s): Hatanaka A, Yamazoe M, Sale J E, Takata M, Yamamoto K., Kitao H, Sonoda E, Kikuchi K, Yonetani Y, Takeda S
  • Journal Title: Molecular Cell Biology 25 Pages: 1124-1134
• [Journal Article] Multiple repair pathways mediate tolerance to chemotherapeutic cross-linking agents in vertebrate cells. (2005)
  • Author(s): Nojima K, Hochegger H, Saberi A, Fukushima T, Kikuchi K, Yoshimura M, Orelli B, Bishop DK, Hirano S, Ohzeki M, Ishiai M, Yamamoto K, Takata M, Arakawa H, Buerstedde JM, Yamazoe M, Kawamoto T, Araki K, Takahashi JA, Hashimoto N, Takeda S, Sonoda E
  • Journal Title: Cancer Research 65 Pages: 11704-11711
• [Journal Article] PTIP promotes DNA double-strand break repair through homologous recombination.
  • Author(s): Wang X, Takenaka K, Takeda S
  • Journal Title: Genes Cells (in press)
  • Peer Reviewed
• [Journal Article] Cooperative Roles of Vertebrate Fbh1 and Blm DNA Helicases in Avoidance bf Crossovers during Recombination Initiated by Replication Fork Collapse.
  • Author(s): Kohzaki M, Hatanaka A, Sonoda E, Yamazoe M, Kikuchi K, Trung NV, Szuts D, Sale JE, Shinagawa H, Watanabe M, Takeda S
  • Journal Title: Mol Cell Biol (in Dress)
• [Journal Article] RAD18 and poly[ADP ribose]polymerase independently suppress the access of nonhomologous end joining to double strand breaks and facilitate homologous recombination mediated repair.
  • Author(s): Saberi A, Hochegger H, Szuts D, Lan L, Yasui A, Sale JE, Taniguchi Y, Murakawa Y, Hua W, Yokomori K, Helleday T, Teraoka H, Arakawa H, Buerstedde JM, Takeda S
  • Journal Title: Mol Cell (in press)
• [Presentation] Analysis of Immunoglobulin V Gene Diversification in the Chicken DT40 B Lymphocyte Line Reveals the Role of DNA Polymerases in Homologous Recombination and Translesion DNA Synthesis (2010)
  • Author(s): Takeda S
  • Organizer: International Conference on Radiation and Cancer Biology at Nagasaki 2010
  • Place of Presentation: Nagasaki University, Nagasaki(長崎県)
  • Year and Date: 2010-02-19
• [Presentation] Interactions between the RNF8 and RAD18 " Differential Functional E3 ubiquitin ligases in Translesion DNA Synthesis and DSB Repair " (2010)
  • Author(s): Takeda S
  • Organizer: EMBO-Recombination Mechanism
  • Place of Presentation: Il Ciocco, Italy(発表予定だったが、直前に家族の事情によりキャンセル)
• [Presentation] Chemical Genetic Method to Detect Environmental Mutagens and to Screen Anti-Malignant Chemical Compounds (2009)
  • Author(s): Takeda S
  • Organizer: The 40th International Symposium of the Princess Takamatsu Cancer Research Fund
  • Place of Presentation: Hotel Grand Palace(東京都)
  • Year and Date: 2009-11-11
• [Presentation] Systematic Reverse Genetic Study of DNA Damage Response, Using the Chicken DT40 Cells (2009)
  • Author(s): Takeda S
  • Organizer: Annual Meeting of the Korean Society for Molecular and Cellular Biology
  • Place of Presentation: COEX Center, Seoul(韓国)
  • Year and Date: 2009-10-16
• [Presentation] DNAを損傷するタイプの抗がん治療時に機能するDNA修復分子 (2009)
  • Author(s): 武田俊一
  • Organizer: 癌学会学術総会
  • Place of Presentation: パシフィコ横浜,横浜,(神奈川)
  • Year and Date: 2009-10-02
• [Presentation] Reverse Genetic Analysis of DNA polymerases in TLS (2009)
  • Author(s): Takeda S
  • Organizer: Gordon Research Conference
  • Place of Presentation: Colby-Sawyer College, New London, New Hampshire(アメリカ)
  • Year and Date: 2009-08-11
• [Presentation] Reverse Genetic Study of RAD51 mediators, BRCAI, BRCA2, RAD51 paralogs, RAD52, Swsl and Sfrl (2009)
  • Author(s): Takeda S
  • Organizer: FASEB Genetic Recombination and Genome Rerrangements
  • Place of Presentation: Snowmass Village, Colorado(アメリカ)
  • Year and Date: 2009-08-06
• [Presentation] A novel approach using DNA repair-deficient chicken DT40 cell lines for screening and char acterizing the genotoxicity of chemical compounds and environmental contaminants (2009)
  • Author(s): Takeda S
  • Organizer: Chromosome Dynamics and Genome Stability
  • Place of Presentation: Villars sur Ollon(スイス)
  • Year and Date: 2009-05-16
• [Presentation] Regulation of DNA damage response by ubiquitin ligases (2009)
  • Author(s): Takeda S
  • Organizer: DNA Repair
  • Place of Presentation: クレタ島(ギリシャ)
  • Year and Date: 2009-04-22
• [Presentation] Analysis of Genome Maintenance Using Chemical Genetics (2009)
  • Author(s): 武田俊一
  • Organizer: 金沢がん生物学国際シンポジウム2009
  • Place of Presentation: 金沢(石川)
  • Year and Date: 2009-02-19
• [Presentation] A Critical Role, for the Ubiquitin-Conjugating Enzymes-Ubc13 and RNF8 in Homologous Recombination (2009)
  • Author(s): Takeda S
  • Organizer: Gordon Research Conference
  • Place of Presentation: Ventura(アメリカ)
  • Year and Date: 2009-02-10
• [Presentation] Detect Environmental Mutagens and to "Chemical Genetic Method to Screen Anti-Malignant Chemical Compounds " (2009)
  • Author(s): Takeda S
  • Organizer: The 40th International Symposium of the Princess Takamatsu Cancer Research Fund
  • Place of Presentation: Hotel Grand Palace, Tokyo
• [Presentation] DNA polymerases in TLS" Gordon Research "Reverse Genetic Analysis of Conference (2009)
  • Author(s): Takeda S
  • Organizer: Colby-Sawyer College, New London
  • Place of Presentation: New Hampshire, USA
• [Presentation] RAD51 mediators, BRCA1, BRCA2, RAD51 "Reverse Genetic Study of paralogs, RAD52, Sws1, and Sfr1" (2009)
  • Author(s): Takeda S
  • Organizer: FASEB Genetic Recombination and Genome Rerrangements
  • Place of Presentation: Snowmass Village, Colorado, USA
• [Presentation] repair-deficient chicken DT40 cell "A novel approach using DNA lines for screening and characterizing the genotoxicity of chemical compounds and environmental contaminants" (2009)
  • Author(s): Takeda S
  • Organizer: Chromosome Dynamics and Genome Stability, Villars sur Ollon
  • Place of Presentation: Switzerland
• [Presentation] response by ubiquitin ligases " DNA (2009)
  • Author(s): Takeda S
  • Organizer: Regulation of DNA damage Repair
  • Place of Presentation: Crete, Greece
• [Presentation] Ubiquitin-Conjugating Enzymes Ubc13 and " A Critical Role for the RNF8 in Homologous Recombination " (2009)
  • Author(s): Takeda S
  • Organizer: Gordon Research Conference
  • Place of Presentation: Ventura, USA
• [Presentation] ニワトリBリンパ細胞を使ったDNA損傷応答機構の遺伝学的解析 (2008)
  • Author(s): 武田俊一
  • Organizer: 放射線防護センターシンポジウム
  • Place of Presentation: 稲毛(千葉)
  • Year and Date: 2008-12-16
• [Presentation] A critical role for the ubiquitin-conjugating enzymes Ubc13 and RNF8 in homologous recombination (2008)
  • Author(s): 武田俊一
  • Organizer: 3R Symposium 2008
  • Place of Presentation: つま恋(静岡)
  • Year and Date: 2008-10-30
• [Presentation] A critical role for the ubiquitin-conjugating eniymes Ubc13 and RNF8 in homologous recombination (2008)
  • Author(s): Takeda S
  • Organizer: Genome Stability in Health and Disease
  • Place of Presentation: Tel Aviv(イスラエル)
  • Year and Date: 2008-09-23
• [Presentation] Collabofation of BRCA1 and CtIP in cellular tolerance totopoisomerase poisons (2008)
  • Author(s): rakeda S
  • Organizer: Gordon Research Conference
  • Place of Presentation: Oxford(イギリス)
  • Year and Date: 2008-07-22
• [Presentation] The role of anti-recombination in replication-fork collapse (2008)
  • Author(s): tkeda S
  • Organizer: Molecular and Clinical Mechanisms in Bloom's Syndome and Related Diosorders
  • Place of Presentation: Chicago(アメリカ)
  • Year and Date: 2008-05-28
• [Presentation] Multiple functions of Mie11/Rad50/Nbs1 complex (2008)
  • Author(s): Takeda S
  • Organizer: 2008 EMBO Recombination Mechamisms
  • Place of Presentation: I1 Ciocco (イタリア)
  • Year and Date: 2008-05-20
• [Presentation] BRCA1-CTIP complex plays a novel role in cellular tolerance to double-strand breaks associated with polypeptides in the presence of topoisomerase poisons. (2008)
  • Author(s): Takeda S, Nakamura K, Taniguchi Y
  • Organizer: Abcam, Maintenance of Genome Stability Conference 2008
  • Place of Presentation: Puerto Vallarta, Mexico
• [Presentation] A critical role for the ubiquitin-conjugating enzyme Ubc13 in initiating homologous recombination (2007)
  • Author(s): Takeda S
  • Organizer: CNIO Cancer Conferences
  • Place of Presentation: Madrid, Spain,
  • Year and Date: 2007-11-06
• [Presentation] A critical role for the ubiquitin-conjugating enzyme UBC13 in initiating homologous recombination. (2007)
  • Author(s): Takeda S
  • Organizer: FASEB Genetic Recombination & Genome Rearrangements
  • Place of Presentation: Snowmass Village, Colorado, USA
  • Year and Date: 2007-07-29
• [Presentation] 遺伝子破壊メダカ作製系の樹立,淡路,兵庫,5月17日,2007.(招待、特別講演) (2007)
  • Author(s): 武田俊一
  • Organizer: 第5回幹細胞シンポジウム
  • Place of Presentation: 兵庫県淡路市
  • Year and Date: 2007-05-17