| Budget Amount *help |
¥48,500,000 (Direct Cost: ¥48,500,000)
Fiscal Year 2009: ¥9,700,000 (Direct Cost: ¥9,700,000)
Fiscal Year 2008: ¥9,700,000 (Direct Cost: ¥9,700,000)
Fiscal Year 2007: ¥9,700,000 (Direct Cost: ¥9,700,000)
Fiscal Year 2006: ¥9,700,000 (Direct Cost: ¥9,700,000)
Fiscal Year 2005: ¥9,700,000 (Direct Cost: ¥9,700,000)
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| Research Abstract |
Mammalians have multiple DNA polymerases for translesion DNA synthesis (TLS), which show different specificities for lesions to bypass. In order to investigate how a TLS DNA polymerase appropriate for bypassing a given DNA lesion is recruited, we studied on the interactions of human Polκ, Polη or Polι with other proteins. Polκ, Polη and Polι each interact with a C-terminal region of REV1 (REV1-CTD). We found 1) interaction of Polk with REV1-CTD is necessary for its functions, 2) the interaction of Polη is not required for the enzyme to bypass UV-induced lesions, but it may be required for the enzyme to bypass other lesions. Polη interacts with PCNA and also with Rad6-Rad18 complex that ubiquitinates PCNA in DNA-damaged cells. Therefore, Polη likely has a priority to bind Ub-PCNA when Rad6-Rad18 complex modifies PCNA in a stalled replication fork. When Polη cannot bypass the lesion in the stalled replication fork, switching from Polη to another TLS DNA polymerase may occur through their interactions with REV1-CTD.
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