| Project/Area Number |
17014016
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| Research Category |
Grant-in-Aid for Scientific Research on Priority Areas
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| Allocation Type | Single-year Grants |
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| Review Section |
Biological Sciences
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| Research Institution | The University of Tokyo |
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Principal Investigator |
MIYAJIMA Atsushi The University of Tokyo, 分子細胞生物学研究所, 教授 (50135232)
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| Co-Investigator(Kenkyū-buntansha) |
TANAKA Minoru 東京大学, 分子細胞生物学研究所, 助教 (80321909)
ITOH Tohru 東京大学, 分子細胞生物学研究所, 助教 (50396917)
TANIMIZU Naoki 東京大学, 分子細胞生物学研究所, 助教 (00333386)
SEKINE Keisuke 東京大学, 分子細胞生物学研究所, 助手 (00323569)
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| Co-Investigator(Renkei-kenkyūsha) |
TANAKA Minoru 東京大学, 分子細胞生物学研究所, 助教 (80321909)
ITOH Tohru 東京大学, 分子細胞生物学研究所, 助教 (50396917)
TANIMIZU Naoki 東京大学, 分子細胞生物学研究所, 助教 (00333386)
SAIJIO Eiko 東京大学, 分子細胞生物学研究所, 技術職員 (60376647)
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| Project Period (FY) |
2005 – 2009
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| Project Status |
Completed (Fiscal Year 2009)
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| Budget Amount *help |
¥48,900,000 (Direct Cost: ¥48,900,000)
Fiscal Year 2009: ¥10,400,000 (Direct Cost: ¥10,400,000)
Fiscal Year 2008: ¥10,400,000 (Direct Cost: ¥10,400,000)
Fiscal Year 2007: ¥8,400,000 (Direct Cost: ¥8,400,000)
Fiscal Year 2006: ¥8,400,000 (Direct Cost: ¥8,400,000)
Fiscal Year 2005: ¥11,300,000 (Direct Cost: ¥11,300,000)
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| Keywords | 肝細胞 / シグナル伝達 / モノクローナル抗体 / サイトカイン / 癌細胞 / 発生 / 分化 / 幹細胞 |
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| Research Abstract |
We prepared a panel of monoclonal antibodies for cell surface proteins on various liver cells, and developed culture systems using isolated cell populations. By applying those tools we characterized fetal liver hepatoblasts and adult liver stem/progenitor cells. We also characterized sinusoidal endothelial cells and stellate cells that constitute sinusoids. Liver mesothelial cells were also isolated and shown to support proliferation of fetal hepatocytes. Transcription factors and cell membrane proteins involved in hepatocyte differentiation were also identified and characterized.
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