Abnormal Differentiation in Cancer Cells and Intracellular Signaling

• Project/Area Number: 17014066
• Research Category: Grant-in-Aid for Scientific Research on Priority Areas
• Allocation Type: Single-year Grants
• Review Section: Biological Sciences
• Research Institution: Hiroshima University
• Principal Investigator: KIKUCHI Akira Hiroshima University, 医学系研究科, 教授 (10204827)
• Co-Investigator(Kenkyū-buntansha): YAMAMOTO Hideki 大阪大学, 医学系研究科, 准教授 (20372691) ; SATO Akira 大阪大学, 医学系研究科, 助教 (70464302) ; HINO Shin-ichirou 広島大学, 医歯薬学総合研究科, 助手 (00372699) ; KISHIDA Michiko 広島大学, 医歯薬学総合研究科, 助手 (40274089)
• Project Period (FY): 2005 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥142,700,000 (Direct Cost: ¥142,700,000); Fiscal Year 2009: ¥29,200,000 (Direct Cost: ¥29,200,000); Fiscal Year 2008: ¥29,200,000 (Direct Cost: ¥29,200,000); Fiscal Year 2007: ¥28,200,000 (Direct Cost: ¥28,200,000); Fiscal Year 2006: ¥28,200,000 (Direct Cost: ¥28,200,000); Fiscal Year 2005: ¥27,900,000 (Direct Cost: ¥27,900,000)
• Keywords: がん / Wnt / シグナル伝達 / 分化 / 極性 / 接着 / 転移 / がん遺伝子 / がん抑制遺伝子 / Wnt5a / 胃癌 / 細胞接着斑 / インテグリン / 細胞周期 / ラミニン5γ2 / 細胞運動 / JunD / 前立腺癌 / Wnt-5a / 翻訳後修飾 / 胃がん / β-カテニン / PKA / SUMO / PIASy / GSK-3 / h-prune

Research Abstract

In this study we focused in Wnt signaling among many intracellular signaling pathways involved in tumorigenesis. We analyzed the molecular mechanisms of tumorigenesis by abnormality in Wnt signaling in the light of differentiation and polarity. The following points are clarified. (1) Wnt proteins were purified and the roles of their post-translational modifications were clarified. (2) Receptor-mediated endocytic routes played a role in the selective activation of multiple Wnt signaling. (3) Wnt5a signaling regulated cell migration and cell polarity. (4) Abnormalities in Wnt5a signaling were involved in aggressiveness of human cancer. (5) Novel binding proteins to GSK-3, a protein kinase, were identified, and they were involved in the regulation of microtubule dynamics and focal adhesion. (6) Dvl regulated spindle dynamics and spindle assemble dynamics in mitosis.

Research Products

• [Journal Article] Binding of APC and disheveled mediates Wnt5a-regulated focal adhesion dynamics in migrating cells. (2010)
  • Author(s): Matsumoto,S., Fumoto,K. Okamoto,T. Kaibuchi,K., Kikuchi,A
  • Journal Title: EMBO J. 29 Pages: 1192-1204
• [Journal Article] Wnt5a signaling is involved in the aggressiveness of prostate cancer and expression of metalloproteinase. (2010)
  • Author(s): Yamamoto,H., Oue,N., Sato,A., Hasegawa,Y., Matsubara,A., Yamamoto,H., Yasui,W., Kikuch,A
  • Journal Title: Oncogene 29 Pages: 2036-2046
  • Peer Reviewed
• [Journal Article] LRP6 is internalized by Dkk1 to suppress its phosphorylation in the lipid raft and is recycled for reuse. (2010)
  • Author(s): Sakane,H., Yamamoto,H., Kikuchi,A
  • Journal Title: J.Cell Sci. 123 Pages: 360-368
  • Peer Reviewed
• [Journal Article] Wnt5a regulates distinct signaling pathways by binding to Frizzled2. (2010)
  • Author(s): Sato,A., Yamamoto,H., Sakane,H., Koyama,H., Kikuchi,A
  • Journal Title: EMBO J. 29 Pages: 41-54
  • Peer Reviewed
• [Journal Article] Wnt5a regulates distinct signaling pathways by binding to Frizzled2. (2010)
  • Author(s): Sato, A., et.al.
  • Journal Title: EMBO J. Volume: 29 Pages: 41-54
  • Peer Reviewed
• [Journal Article] LPR6 is internalized by Dkk1 to suppress its phosphorylation in the lipid raft and is recycled for reuse. (2010)
  • Author(s): Sakane, H., et.al.
  • Journal Title: J.Cell Sci. Volume: 123 Pages: 360-368
  • Peer Reviewed
• [Journal Article] Wnt5a signaling is involved in the aggressiveness of prostate cancer and expression of metalloproteinase. (2010)
  • Author(s): Yamamoto, H., et.al.
  • Journal Title: Oncogene Volume: 29 Pages: 2036-2046
  • Peer Reviewed
• [Journal Article] Axin localizes to the centrosome and is involved in microtubule nucleation. (2009)
  • Author(s): Fumoto,K., Kadono,M., Izumi,N., Kikuchi,A
  • Journal Title: EMBO R. 10 Pages: 606-613
  • Peer Reviewed
• [Journal Article] Selective activation mechanisms of Wnt signaling pathways. (2009)
  • Author(s): Kikuchi,A., Yamamoto,H., Sato,A
  • Journal Title: Trends Cell Biol. 19 Pages: 119-129
  • Peer Reviewed
• [Journal Article] Laminin・2 mediates Wnt5a-induced invasion of gastric cancer cells. (2009)
  • Author(s): Yamamoto,H., Kitadai,.Y., Yamamoto,H., Oue,N., Ohdan,H., Yasui,W., Kikuchi,A
  • Journal Title: Gastroenterology 137 Pages: 242-252
  • Peer Reviewed
• [Journal Article] CHD8 suppreses p53-mediated apoptosis through histone H1 recruotment during early embryogenesis. (2009)
  • Author(s): Nishiyama,M., Oshikawa,K., Tukada,Y., Nakagawa,T., Iemura,S., Natsume,T., FanY., Kikuchi,A., Skoultchi,A.I., Nakayama,K
  • Journal Title: Nat.Cell Biol. 11 Pages: 172-182
  • Peer Reviewed
• [Journal Article] Laminin γ2 Mediates Wnt5a-induced Invasion of Gastric Cancer Cells. (2009)
  • Author(s): Yamamoto, H., et.al.
  • Journal Title: Gastroenterology Volume: 137 Pages: 242-252
  • Peer Reviewed
• [Journal Article] Axin localizes to the centrosome and is involved in microtubule nucleation. (2009)
  • Author(s): Fumoto, K., et.al.
  • Journal Title: EMBO R. Volume: 10 Pages: 606-613
  • Peer Reviewed
• [Journal Article] CHD8 suppresses p53-mediated apoptosis through histone H1 recruit-ment during early embryogenesis. (2009)
  • Author(s): Nishiyama, M., et.al.
  • Journal Title: Nat.Cell Biol. Volume: 11 Pages: 172-182
  • Peer Reviewed
• [Journal Article] Retinoic acid induces discrete Wnt-signaling-dependent differentiation in F9 cells. (2009)
  • Author(s): Inoue, A., et.al.
  • Journal Title: Biophys.Biochem., Res.Commun. Volume: 390 Pages: 564-569
  • Peer Reviewed
• [Journal Article] Fbw7 promotes ubiquitin-dependent degradation of c-Myb : involvement of GSK3-mediated phosphorylation of Thr-572 in mouse c-Myb. (2009)
  • Author(s): Kitagawa K., et.al.
  • Journal Title: Oncogene. Volume: 28 Pages: 2393-2405
  • Peer Reviewed
• [Journal Article] Wnt-Ryk signaling mediates axon growth inhibition andlimits functional recovery after spinal cord injury. (2009)
  • Author(s): Miyashita, T., et.al.
  • Journal Title: J Neurotrauma. Volume: 26 Pages: 955-964
  • Peer Reviewed
• [Journal Article] Laminin γ2 mediates Wnt5a-induced invasion of Gastric Cancer Cells (2009)
  • Author(s): Yamamoto, H., et.al.
  • Journal Title: Gastroenterology (in press)
  • Peer Reviewed
• [Journal Article] Axin localizes to the centrosome and is involved in microtubule nucleation (2009)
  • Author(s): Fumoto, K., et.al.
  • Journal Title: EMBO R (in press)
  • Peer Reviewed
• [Journal Article] Selective activation of mechanisms of Wnt signaling pathways (2009)
  • Author(s): Kikuchi, A., et.al.
  • Journal Title: Trends Cell Biol 19 Pages: 119-129
  • Peer Reviewed
• [Journal Article] CHD8 suppresses p53-mediated apoptosis through histone H1 recruitment during early embryogenesis (2009)
  • Author(s): Nishiyama, M., et.al.
  • Journal Title: Nat. Cell Biol 11 Pages: 172-182
  • Peer Reviewed
• [Journal Article] Polycomb-group complex 1 acts as an E3 ubiquitin ligase for Geminin to sustain hematopoietic stem cell activity. (2008)
  • Author(s): Ohtsubo,M., Yasunaga,S., Ohno,Y., Tsumura,M., Okada,S., Ishikawa,N., Shirao,K., Kikuchi,A., Nishitani,H., Kobayashi,M., Takihara,Y
  • Journal Title: Proc.Natl.Acad.Sci.USA. 105 Pages: 10396-10401
  • Peer Reviewed
• [Journal Article] AIP regulates stability of Aurora-A at early mitotic phase coordinately with GSK-3・. (2008)
  • Author(s): Fumoto,K., Lee, P-C., Saya,H., Kikuchi,A
  • Journal Title: Oncogene 27 Pages: 4478-4487
  • Peer Reviewed
• [Journal Article] Tumor formation due to abnormalities in the ・-catenin-independent pathway of Wnt signaling. (2008)
  • Author(s): Kikuchi,A. Yamamoto,H
  • Journal Title: Cancer Sci. 99 Pages: 202-208
  • Peer Reviewed
• [Journal Article] GSK-3・ regulates proper mitotic spindle formation in cooperation with a component of the がγ-tubulin ring complex, GCP5. (2008)
  • Author(s): Izumi,N., Fumoto,K., Izumi,S., Kikuchi,A
  • Journal Title: J.Biol.Chem. 283 Pages: 12981-12991
  • Peer Reviewed
• [Journal Article] IGFBP-4 is a canonical Wnt inhibitor that promotes cardiac myogenesis. (2008)
  • Author(s): Zhu,W., Shiojima,I., Ito,Y., Li,Z., Ikeda,H., Yoshida,M., Naito,A., Nishi,J., Ueno,H., Umezawa,A., Minamino1,T., Nagai,T., Kikuchi,A., Asashima,M., Komuro,I
  • Journal Title: Nature 454 Pages: 345-349
  • Peer Reviewed
• [Journal Article] Wnt3a and Dkk1 regulate distinct internalization pathways of LRP6 to tune the activation of ・-catenin signaling. (2008)
  • Author(s): Yamamoto,H., Sakane,H., Yamamoto,H., Michiue,T., Kikuch,A
  • Journal Title: Dev. Cell 15 Pages: 37-48
• [Journal Article] IFN-induced transmembrane protein1 promotes the invasion at the early stage of head and neck cancer progression (2008)
  • Author(s): Hatano, H., et.al.
  • Journal Title: Cli. Cancer Res 14 Pages: 6097-6105
  • Peer Reviewed
• [Journal Article] Polycomb-group complex1 acts as an E3 ubiquitin ligase for Geminin to sustain hematopoietic stem cell activity (2008)
  • Author(s): Ohtsubo, M., et.al.
  • Journal Title: Proc. Natl. Acad. Sci. USA 105 Pages: 10396-10401
  • Peer Reviewed
• [Journal Article] AIP regulates stability of Aurora-A at early mitoticphase coordinately with GSK-3β (2008)
  • Author(s): Fumoto, K., et.al.
  • Journal Title: Oncogene 27 Pages: 4478-4487
  • Peer Reviewed
• [Journal Article] IGFBP-4 is an inhibitor of canonical Wnt signaling reauired for cardiocrenesis (2008)
  • Author(s): Zhu, W., et.al.
  • Journal Title: Nature 454 Pages: 345-349
  • Peer Reviewed
• [Journal Article] Wnt3a and Dkk1 regulate distinct internalization pathways of LRP6 to tune the activation of β-catenin signaling (2008)
  • Author(s): Yamamoto, H., et.al.
  • Journal Title: Dev. Cell 15 Pages: 37-48
  • Peer Reviewed
• [Journal Article] GSK-3β regulates proper mitotic spindle formation in cooperation with a component of the γ-tubulin ring complex, GCP5 (2008)
  • Author(s): Izumi, N., et.al.
  • Journal Title: J. Biol. Chem 283 Pages: 12981-12991
  • Peer Reviewed
• [Journal Article] Tumor formation due to abnormalities in the β-catenin-independent pathway of Wnt signaling (2008)
  • Author(s): Kikuchi, A., et.al.
  • Journal Title: Cancer Sci 99 Pages: 202-208
  • NAID: 10024001624
  • Peer Reviewed
• [Journal Article] Tumor formation due to abnormalities in the β-catenin-independent pathway of Wnt siqnaling. (2008)
  • Author(s): Kikuchi, A. Yamamoto, H.
  • Journal Title: Cancer Sci. 99 Pages: 202-208
  • Peer Reviewed
• [Journal Article] GSK-3β egulates proper mitotic spindle formationin cooperation with a component of the r-tubulinring complex, GCP5. (2008)
  • Author(s): Izumi, N., et. al.
  • Journal Title: J. Biol. Chem. 288 Pages: 12981-12991
  • Peer Reviewed
• [Journal Article] AIP regulates stability of Aurora-A at early mitotic phase coordinately with GSK-3β. (2008)
  • Author(s): Fumoto, K., et. al.
  • Journal Title: Oncogene (in press)
  • Peer Reviewed
• [Journal Article] IFITM1 promotes the invasion at the early stage of head and neck cancer progression. (2008)
  • Author(s): Hatano, H., et. al.
  • Journal Title: Gli. Cancer Res. (in press)
  • Peer Reviewed
• [Journal Article] Wnt3a and Dkkl regulate internalization of LRP6 differently to tune β-catenin pathway・ (2008)
  • Author(s): Yamamoto, H., et. al.
  • Journal Title: Dev. Cell (in press)
  • Peer Reviewed
• [Journal Article] Glycosylation and palmitoylation of Wnt-3a are coupled to produce an active form of Wnt-3a. (2007)
  • Author(s): Komekado,H., Yamamoto,H., Chiba,T., Kikuchi,A
  • Journal Title: Genes Cells 12 Pages: 521-534
  • Peer Reviewed
• [Journal Article] Regulation of Wnt signaling by receptor-mediated endocytosis. (2007)
  • Author(s): Kikuchi,A. Yamamoto,H
  • Journal Title: J.Biochem. 141 Pages: 443-451
  • NAID: 10020099687
  • Peer Reviewed
• [Journal Article] Dvl regulates endo- and exocytotic processes through binding to synaptotagmin. (2007)
  • Author(s): Kishida,S., Hamao,K., Inoue,M., Hasegawa,M., Matsuura,Y., Mikoshiba,K., Fukuda,M., Kikuchi,A
  • Journal Title: Genes Cells 12 Pages: 49-61
  • Peer Reviewed
• [Journal Article] Post-translational palmitoylation and glycosylation of Wnt-5a are necessary for its signaling. (2007)
  • Author(s): Kurayoshi,M., Yamamoto,H., Takada,S., Izumi,S., Kikuchi,A
  • Journal Title: Biochem.J. 402 Pages: 515-523
  • Peer Reviewed
• [Journal Article] Non-covalent binding of SUMO protease to SUMO is necessary for enzymatic activities and cell growth. (2007)
  • Author(s): Ihara,M., Koyama,H., Uchimura,Y., Saitoh,H., Kikuchi,A
  • Journal Title: J.Biol.Chem. 282 Pages: 16465-16475
  • Peer Reviewed
• [Journal Article] The DIX domain of Dishevelled confers Wnt signaling by dynamic polymerization. (2007)
  • Author(s): Schwarz-Romond,T., Fiedler1,M., Shibata,N., Butler,P.J.G., Kikuchi,A., Higuchi,Y., Bienz,M.
  • Journal Title: Nat.Strut.Mol.Biol. 14 Pages: 484-492
  • Peer Reviewed
• [Journal Article] Multiplicity of the interactions of Wnt proteins and their receptors. (2007)
  • Author(s): Kikuchi,A., Kishida,S., Yamamoto,H
  • Journal Title: Cell.Signal. 19 Pages: 659-671
  • Peer Reviewed
• [Journal Article] Post-translational palmitoylation and glycosylation of Wnt-5a are necessary for its signaling. (2007)
  • Author(s): Kurayoshi, M., et. al.
  • Journal Title: Biochem. J. 402 Pages: 515-523
  • Peer Reviewed
• [Journal Article] Dvl regulates endo- and exocytotic processes through binding to synaptotagmin. (2007)
  • Author(s): Kishida, S., et. al.
  • Journal Title: Genes Cells 12 Pages: 49-61
  • Peer Reviewed
• [Journal Article] Glycosylation and palmitoylation of Wnt-3a are coupled to produce an active form of Wnt-3a. (2007)
  • Author(s): Komekado, H., et. al.
  • Journal Title: Genes Cells 12 Pages: 521-534
  • Peer Reviewed
• [Journal Article] Wnt5a modulates glycogen synthase kinase 3 to induce phospshorylation of receptor tyrosine kinase Ror2. (2007)
  • Author(s): Yamamoto, H., et. al.
  • Journal Title: Genes Cells 12 Pages: 1215-1223
  • Peer Reviewed
• [Journal Article] 工ncreased expression of h-prune is associated with tiraior progression and poor survival in gastric cancer. (2007)
  • Author(s): Oue, N., et. al.
  • Journal Title: Cancer Sci. 98 Pages: 1198-1205
  • Peer Reviewed
• [Journal Article] Sprouty2 and Sprouty4 are essential for embryonic morphogenesis and regulation of FGF signaling. (2007)
  • Author(s): Taniguchi, K., et. al.
  • Journal Title: Biochem. Biophys. Res. Commun. 352 Pages: 896-902
  • Peer Reviewed
• [Journal Article] Multiplicity of the interactions of Wnt proteins and their receplors. (2007)
  • Author(s): Kikuchi, A., et. al.
  • Journal Title: Cell. Signal. 19 Pages: 659-671
  • Peer Reviewed
• [Journal Article] Regulation of Wnt signaling by receptor-mediated endocytosis. (2007)
  • Author(s): Kikuchi, A. Yamanoto. H.
  • Journal Title: J. Biochem. 141 Pages: 443-451
  • NAID: 10020099687
  • Peer Reviewed
• [Journal Article] The DIX domain of Dishevelled confers Wnt signaling by dynamic polymerization. (2007)
  • Author(s): Schwarz-Romond, T., et. al.
  • Journal Title: Nat. Strut. Mol. Biol. 14 Pages: 484-492
  • Peer Reviewed
• [Journal Article] Non-covalent binding of SUMO protease to SUMO is necessary for enzymatic activities and cell growth. (2007)
  • Author(s): Thara, M., et. al.
  • Journal Title: J. Biol. Chem. 282 Pages: 16465-16475
  • Peer Reviewed
• [Journal Article] Dvlregulates endo-and exocytotic processes through binding to synaptotagmin (2007)
  • Author(s): Kishida, S., et al.
  • Journal Title: Genes Cells 12 Pages: 49-61
• [Journal Article] Post-translational palmitoylation and glcosylation of Wnt-5a are necessary for its signaling (2007)
  • Author(s): Kurayoshi, M., et al.
  • Journal Title: Biochem. J. 402 Pages: 515-523
• [Journal Article] Sprouty2 and Sprouty4 are essential for embryonic morphogenesis and regulation of FGF signaling. (2007)
  • Author(s): Taniguchi, K., et al.
  • Journal Title: Biochem. Biophys. Res. Commun. 352 Pages: 896-902
• [Journal Article] Glycosylation and palmitoylation of Wnt-3a are coupled to produce an active form of Wnt-3a. (2007)
  • Author(s): Komekado, H., et al.
  • Journal Title: Genes Cells (in press)
• [Journal Article] Multiplicity of the interactions of Wnt Proteins and their receptors. (2007)
  • Author(s): Kikuchi, A., et al.
  • Journal Title: Cell. Signal. (in press)
• [Journal Article] Regulation of Wnt signaling by receptor-mediated endocytosis (2007)
  • Author(s): Kikuchi, A., et al.
  • Journal Title: J. Biochem. (in press)
  • NAID: 10020099687
• [Journal Article] Developmemtal stage-specific, biphasic roles of Wnt/・-catenin signaling in cardiomyogenesis and hematopoiesis. (2006)
  • Author(s): Naito,A., Shiojima,I., Akazawa,H., Hidaka,K., Morisaki,T., Kikuchi,A., Komuro,I
  • Journal Title: Proc.Natl.Acad.Sci.USA. 103 Pages: 19812-19817
  • Peer Reviewed
• [Journal Article] Filopodia formation mediated by receptor tyrosine kinase Ror2 is required for Wnt5a-induced cell migration. (2006)
  • Author(s): Nishita,M., Yoo,S-K., Nomachi,A., Kani,S., Sougawa,N., Ohta,Y., Takada,S., Kikuchi,A., Minami,Y
  • Journal Title: J.Cell Biol. 175 Pages: 555-562
  • Peer Reviewed
• [Journal Article] Expression of Wnt-5a is correlated with aggressiveness of gastric cancer by stimulating cell migration and invasion. (2006)
  • Author(s): Kurayoshi,M., Oue,N., Yamamoto,H., Kishida,M., Inoue,A., Asahara,T., Yasui,W., Kikuchi,A
  • Journal Title: Cancer Res. 66 Pages: 10439-10448
  • Peer Reviewed
• [Journal Article] GSK-3・-regulated interaction of BICD with dynein is involved in microtubule anchorage at centrosome. (2006)
  • Author(s): Fumoto,K., Hoogenraad,C.C., Kikuchi,A
  • Journal Title: EMBO J. 25 Pages: 5670-5682
  • Peer Reviewed
• [Journal Article] Regulation of Wnt signaling by protein-protein interaction and post-translational modifications. (2006)
  • Author(s): Kikuchi,A., Kishida,S., Yamamoto,H
  • Journal Title: Experi.&Mol.Med. 38 Pages: 1-10
  • Peer Reviewed
• [Journal Article] Caveolin is necessary for Wnt-3a-induced internalization of LRP6 and accumulation of ・-catenin. (2006)
  • Author(s): Yamamoto,H., Komekado,H., Kikuchi,A
  • Journal Title: Dev.Cell 11 Pages: 213-223
  • Peer Reviewed
• [Journal Article] Glycogen synthase kinase-3 And h-prune regulate cell migration by modulating focal adhesions. Mol. (2006)
  • Author(s): Kobayashi,T., Hino,S., Oue,N., Asahara,T., Zollo,M., Yasui,W., Kikuchi,A
  • Journal Title: Cell.Biol. 26 Pages: 898-911
  • Peer Reviewed
• [Journal Article] Glycogen synthase kinase-3 Andh-prune regulatecell migration by modulating focal adhesions. (2006)
  • Author(s): Kobayashi T. et al.
  • Journal Title: Mol. Cell. Biol. 26 Pages: 898-911
• [Journal Article] Caveolin is necessary.for Wnt-3a-induced internalization of LRP6 and accumulation of β-catenin. (2006)
  • Author(s): Yamamoto, H. et al.
  • Journal Title: Dev. Cell 11 Pages: 213-223
• [Journal Article] GSK-3β-regulated interaction of BICD with dynein is involved in microtubule anchorage at centrosome. (2006)
  • Author(s): Fumoto, K., et al.
  • Journal Title: EMBO J. 25 Pages: 5670-5682
• [Journal Article] Expression of Wnt-5a is correlated with aggressiveness of gastric cancer by stimulating cell migration and invasion. (2006)
  • Author(s): Kurayoshi, M., et al.
  • Journal Title: Cancer Res. 66 Pages: 10439-10448
• [Journal Article] Filopodia formation mediated by receptor tyrosine kinase Ror2 is required for Wnt5a-induced cell migration (2006)
  • Author(s): Nishita, M. et al.
  • Journal Title: J. Cell Biol. 175 Pages: 555-562
• [Journal Article] Developmemtal stage-specific, biphasic roles of Wnt/β-catenin signaling in cardiomyogenesis and hematopoiesis (2006)
  • Author(s): Naito, A., et al.
  • Journal Title: Proc. Natl. Acad. Sci. USA 103 Pages: 19812-19817
• [Journal Article] Suppression of STAT3 activity by Duplin, Which is a negative regulator of the Wnt signal. (2006)
  • Author(s): Yamashina, K., et al.
  • Journal Title: J. Biochem 139 Pages: 305-314
• [Journal Article] Oogl, anoocyte-specific protein, interacts with Ras and Ras-signaling proteins during early embryogenesis. (2006)
  • Author(s): Tsukamoto, S., et al.
  • Journal Title: Biochem. Biopgys. Res. Commun. 343 Pages: 1105-1112
• [Journal Article] HTLV-I Tax dysregulates β-catenin signaling (2006)
  • Author(s): Tomita, M., et al.
  • Journal Title: J. Virol. 80 Pages: 10497-10505
• [Journal Article] Regulation of Wnt signaling by protein-protein interaction and post-translational modifications. (2006)
  • Author(s): Kikuchi.A., et al.
  • Journal Title: Exp. Mol. Med. 38 Pages: 1-10
• [Journal Article] Glycogen synthase kinase-3 And h-prune regulate cell migration by modulating focal adhesions. (2006)
  • Author(s): Kobayashi, T. et al.
  • Journal Title: Mol.Cell.Biol. 26 Pages: 898-911
• [Journal Article] Suppression of STAT3 activity by Duplin, Which is a negative regulator of the Wnt signal. (2006)
  • Author(s): Yamashina, K. et al.
  • Journal Title: J.Biochem. 139 Pages: 305-314
• [Journal Article] Regulation of Wnt signaling by protein-protein interaction and post-tranlational modifications. (2006)
  • Author(s): Kikuchi, A. et al.
  • Journal Title: Exp.Mol.Med. 38 Pages: 1-10
• [Journal Article] GSK-3 regulates phosphorylation of CRMP-2 and neuronal polarity. (2005)
  • Author(s): Yoshimura,T., Kawano,Y., Arimura,N., Kawabata,S., Kikuchi,A., KaibuchiK
  • Journal Title: Cell. 120 Pages: 137-149
  • Peer Reviewed
• [Journal Article] Phosphorylation of ・-catenin by cyclic AMP-dependent protein kinase stabilizes ・-catenin through inhibition of its ubiquitination. (2005)
  • Author(s): Hino,S-I., Tanji,C., Nakayama,K-I., Kikuchi,A
  • Journal Title: Mol.Cell.Biol. 25 Pages: 9063-9072
  • Peer Reviewed
• [Journal Article] SUMO-1 modification of PIASy, an E3 ligase, is necessary for PIASy-dependent activation of Tcf-4. (2005)
  • Author(s): Ihara,M., Yamamoto,H., Kikuchi,A
  • Journal Title: Mol.Cell.Biol. 25 Pages: 3506-3518
  • Peer Reviewed
• [Journal Article] SUMO-1 modification of PIASy, an E3 ligase, is necessary for PIASy-dependent activation of Tcf-4. (2005)
  • Author(s): Ihara, M. et al.
  • Journal Title: Mol.Cell.Biol 25 Pages: 3506-3518
• [Journal Article] Phosphorylation of β-Catenin by Cyclic AMP-dependent Protein Kinase Stabilizes β-Catenin through Inhibition of Its Ubiquitination. (2005)
  • Author(s): Hino, S-I. et al.
  • Journal Title: Mol.Cell.Biol 25 Pages: 9063-9072
• [Journal Article] GSK-3β regulates phosphorylation of CRMP-2 and neuronal polarity. (2005)
  • Author(s): Yoshimura, T. et al.
  • Journal Title: Cell 120 Pages: 137-149
• [Journal Article] Ubiquitin-interacting motifs of Epsin are involved in the regulation of insulin-dependent endocytosis (2005)
  • Author(s): Sugiyama, S. et al.
  • Journal Title: J.Biochem. 137 Pages: 355-364
• [Journal Article] Implication of galectin-3 in Wnt signaling (2005)
  • Author(s): Shimura, T. et al.
  • Journal Title: Cancer Res. 64 Pages: 3535-3537
• [Presentation] Regulation of Cell adhesion and Migration byWnt5a signaling (2009)
  • Author(s): 菊池章
  • Organizer: 第32回日本分子生物学会年会
  • Place of Presentation: 横浜
  • Year and Date: 2009-12-12
• [Presentation] Regulation of cell adhesion and migration by Wnt5a signaling (2009)
  • Author(s): Kikuchi, A.
  • Organizer: 第32回日本分子生物学会
  • Place of Presentation: 横浜市
  • Year and Date: 2009-12-12
• [Presentation] Wnt signaling and receptor-mediated endocytosis (2009)
  • Author(s): Kikuchi, A.
  • Organizer: Endotrack on the tracks of signalling
  • Place of Presentation: Il Ciocco, Italy
  • Year and Date: 2009-11-09
• [Presentation] Regulation of cell adhesion and migration by Wnt5a signaling (2009)
  • Author(s): Kikuchi, A.
  • Organizer: EMBO Workshop : Wnt Signalling in Development and Disease
  • Place of Presentation: Arolla, Switzerland
  • Year and Date: 2009-08-28
• [Presentation] Wntシグナルによる細胞運動制御とその異常による癌の悪性化 (2008)
  • Author(s): 菊池章
  • Organizer: 第31回日本分子生物学会年会、第81回日本生化学会大会シンポジウム
  • Place of Presentation: 神戸
  • Year and Date: 2008-12-12
• [Presentation] Wntシグナルによる細胞運動制御とその異常による癌の悪性化 (2008)
  • Author(s): 菊池章
  • Organizer: 第31回日本分子生物学会年会、第81回日本生化学会大会
  • Place of Presentation: 神戸
  • Year and Date: 2008-12-12
• [Presentation] 3次元培養を用いたDishevelledによる細胞運動・形態形成制御機構の解析 (2008)
  • Author(s): 松本真司
  • Organizer: 第31回日本分子生物学会年会、第81回日本生化学会大会
  • Place of Presentation: 神戸
  • Year and Date: 2008-12-11
• [Presentation] β-カテニン非依存性のWntシグナルによる細胞応答とがん (2008)
  • Author(s): 菊池章
  • Organizer: 第67回日本癌学会学術総会モーニングレクチャー
  • Place of Presentation: 横浜
  • Year and Date: 2008-10-29
• [Presentation] β-カテニン非依存性のWntシグナルによる細胞応答とがん (2008)
  • Author(s): 菊池章
  • Organizer: 第67回日本癌学会学術総会
  • Place of Presentation: 名古屋
  • Year and Date: 2008-10-29
• [Presentation] 脂質ラフトにおけるWntシグナルの活性制御 (2008)
  • Author(s): 山本英樹
  • Organizer: 第67回日本癌学会学術総会
  • Place of Presentation: 名古屋
  • Year and Date: 2008-10-28
• [Presentation] Wnt5aはlaminin-5γ2の発現を介して胃癌細胞の転移能を促進する (2008)
  • Author(s): 山本英喜
  • Organizer: 第67回日本癌学会学術総会
  • Place of Presentation: 名古屋
  • Year and Date: 2008-10-28
• [Presentation] Aggressiveness of cancer induced by abnormality of Wnt signaling. (2007)
  • Author(s): Kikuchi, A.
  • Organizer: The Japan-Korea j oint meeting on Wnt signaling and human diseases.
  • Place of Presentation: Seoul, Korea
  • Year and Date: 2007-10-26
• [Presentation] Spatial and temporal regulation of Glycogen Synthase kinase-3 and Cellular function. (2007)
  • Author(s): Kikuchi, A.
  • Organizer: 2007 Annual meeting of Korean Society of Medical Biochemistry and Molecular Biology.
  • Place of Presentation: Seoul, Korea
  • Year and Date: 2007-10-25
• [Presentation] Spatial and temporal regulation of Glycogen Svnthase kinase-3 and Cellular function. (2007)
  • Author(s): Kikuchi, A.
  • Organizer: 2007 Annual meeting of Korean Society of Medical Biochemistry and Molecular Biology.
  • Place of Presentation: Seoul, Korea
  • Year and Date: 2007-10-25
• [Presentation] Wntシグナルによるがんの悪性化 (2007)
  • Author(s): 菊池章
  • Organizer: 第66回日本癌学会学術総会モーニングレクチャー
  • Place of Presentation: 横浜
  • Year and Date: 2007-10-05
• [Presentation] Wnt-5aによる細胞運動能の促進と胃癌の悪性化 (2007)
  • Author(s): 菊池章
  • Organizer: 第65回日本癌学会学術総会シンポジウム
  • Place of Presentation: 横浜
  • Year and Date: 2007-09-30
• [Presentation] Regulation of Wnt signaling by receptor-mediated endocytosis. (2007)
  • Author(s): Kikuchi, A.
  • Organizer: Sympoj ium of Wnt signaling in development and disease.
  • Place of Presentation: Berlin, Germany
  • Year and Date: 2007-09-14
• [Presentation] Regulation of Wnt signaling by receptor-mediated endocytosis. (2007)
  • Author(s): Kikuchi, A.
  • Organizer: Wnt Signaling in Development and Disease.
  • Place of Presentation: Berlin, Germany
  • Year and Date: 2007-09-13
• [Presentation] Expression of Wnt5a is correlated with aggressiveness of gastric and prostate cancer by stimulating cell migration and invasion. (2007)
  • Author(s): Kikuchi, A., Yamamoto,H
  • Organizer: Traditional Wnt meeting.
  • Place of Presentation: La Jolla. California, USA
  • Year and Date: 2007-06-22
• [Presentation] Expression of Wnt5a is correlated with aggressiveness of gastric and prostate cancer by stimulating cell miqration andinvasion. (2007)
  • Author(s): Kikuchi. A. Yamamoto. H.
  • Organizer: Traditional Wnt meeting.
  • Place of Presentation: La Jolla.California, USA
  • Year and Date: 2007-06-22
• [Presentation] Regulation of Cellular functions by Wnt signaling. (2006)
  • Author(s): Kikuchi, A.
  • Organizer: Cell Signaling and Protein Networking.
  • Place of Presentation: Seoul, Korea
  • Year and Date: 2006-05-18
• [Presentation] Caveolin-mediated endocytosis of LRP6 is required for activation of Wnt/・-catenin pathway. (2006)
  • Author(s): Kikuchi, A.
  • Organizer: Keystone symposia, Wnt and・-Catenin Signaling in Development and Disease.
  • Place of Presentation: Snowbird Resort, Snowbird, Utah, USA
• [Remarks] ホームページ等
  • URL: http://www.med.osaka-u.ac.jp/pub/molbiobc/