Cell Polarity Signaling and Cancer

• Project/Area Number: 17014076
• Research Category: Grant-in-Aid for Scientific Research on Priority Areas
• Allocation Type: Single-year Grants
• Review Section: Biological Sciences
• Research Institution: Yokohama City University
• Principal Investigator: OHNO Shigeo Yokohama City University, 医学研究科, 教授 (10142027)
• Co-Investigator(Kenkyū-buntansha): SUZUKI Atsushi 横浜市立大学, 大学院・医学研究科, 准教授 (00264606) ; AKIMOTO Kazunori 横浜市立大学, 医学部, 助教 (70285104) ; HIROSE Tomonori 横浜市立大学, 医学部, 助教 (20381668) ; MIZUNO Keiko 横浜市立大学, 医学部, 助手 (90221803)
• Project Period (FY): 2005 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥328,800,000 (Direct Cost: ¥328,800,000); Fiscal Year 2009: ¥66,100,000 (Direct Cost: ¥66,100,000); Fiscal Year 2008: ¥66,100,000 (Direct Cost: ¥66,100,000); Fiscal Year 2007: ¥66,300,000 (Direct Cost: ¥66,300,000); Fiscal Year 2006: ¥66,300,000 (Direct Cost: ¥66,300,000); Fiscal Year 2005: ¥64,000,000 (Direct Cost: ¥64,000,000)
• Keywords: aPKC / MDCK細胞 / PAR-3 / PAR-6 / 上皮細胞 / 前立腺がん / 胃がん / 乳がん / ASIP / 線虫 / 非対称分裂 / 極性 / 上皮

Research Abstract

A series of experiments focusing on the aPKC-PAR complex revealed (1) the new role of PAR1 on the recruitment and organization of extracellular laminin through utrophin/dystroglucan complex. We have also identified ASPP2, known as a mediator of apoptic function of p53, as a partner of the aPKC-PAR complex. (2) In mammary epithelial stem/progenitor cells the aPKC-ErbB2 pathway negatively regulates proliferation. (3) Relationships between aPKC overexpression and the recurrence of prostate cancer, metastasis of stomach cancer, and grade of mammary cancer. Intensive studies on prostate cancer cells revealed the aPKC-IL6 axis. These results reinforce the importance of the understanding of cell polarity for the understanding of cancer cells.

Research Products

• [Journal Article] AAA+ proteins RUVBL1 and RUVBL2 coordinate PIKK family and function in nonsense-mediated mRNA decay. (2010)
  • Author(s): Izumi N, Yamashita A, Iwamatsu A, Kurata R, Nakamura H, Saari B, Hirano H, Anderson P, Ohno S
  • Journal Title: Science Signaling 3(116)
• [Journal Article] AAA+ proteins RUVBL1 and RUVBL2 coordinate PIKK family and function in nonsense-mediated Mrna decay. (2010)
  • Author(s): Inumi N, Yamashita A, Iwamatsu A, Kurata R, Nakamura H, Saari B, Hirano H, Anderson P, Ohno S
  • Journal Title: Science Signaling 3(116)
  • Peer Reviewed
• [Journal Article] A cell polarity protein aPKClambda is required for eye lens formation and growth. (2009)
  • Author(s): Sugiyama Y, Akimoto K, Robinson M L, Ohno S, Quinlan R A
  • Journal Title: Dev Biol 336(2) Pages: 246-256
• [Journal Article] High expression of atypical protein kinase C lambda/iota in gastric cancer as a prognostic factor for recurrence. (2009)
  • Author(s): Takagawa R, Akimoto K, Ichikawa Y, Akiyama H, Kojima Y, Ishiguro H, Inayama Y, Aoki I, Kunisaki C, Endo I, Nagashima Y, Ohno S
  • Journal Title: Ann Surg Oncol 17(1) Pages: 81-88
• [Journal Article] aPKClambda/iota promotes growth of prostate cancer cells in an autocrine manner through transcriptional activation of interleukin-6. (2009)
  • Author(s): Ishiguro H, Akimoto K, Nagashima Y, Kojima Y, Sasaki T, Ishiguro-Imagawa Y, Nakaigawa N, Ohno S, Kubota Y, Uemura H
  • Journal Title: Proc Natl Acad Sci U S A 106(38) Pages: 16369-16374
• [Journal Article] An essential role of the aPKC-Aurora A-NDEL1 pathway in neurite elongation by modulation of microtubule dynamics. (2009)
  • Author(s): Mori D, Yamada M, Mimori-Kiyosue Y, Shirai Y, Suzuki A, Ohno S, Saya H, Wynshaw-Boris A, Hirotsune S
  • Journal Title: Nat Cell Biol 11(9) Pages: 1057-1068
• [Journal Article] Intracellular polarity protein PAR-1 regulates extracellular laminin assembly by regulating the dystroglycan complex. (2009)
  • Author(s): Masuda-Hirata M, Suzuki A, Amano Y, Yamashita K, Ide M, Yamanaka T, Sakai M, Imamura M, Ohno S
  • Journal Title: Genes Cells 14(7) Pages: 835-850
• [Journal Article] Interaction between PAR-3 and the aPKC-PAR-6 complex is indispensable for apical domain development of epithelial cells. (2009)
  • Author(s): Horikoshi Y, Suzuki A, Yamanaka T, Sasaki K, Mizuno K, Sawada H, Yonemura S, Ohno S
  • Journal Title: J. Cell Science 122(10) Pages: 1595-1606
• [Journal Article] SMG-8 and SMG-9, two novel subunits of the SMG-1 complex, regulate remodeling of the mRNA surveillance complex during nonsense-mediated mRNA decay. (2009)
  • Author(s): Yamashita A, Izumi N, Kashima I, Ohnishi T, Saari B, Katsuhata Y, Muramatsu R, Morita T, Iwamatsu A, Hachiya T, Kurata R, Hirano H, Anderson P, Ohno S
  • Journal Title: Genes Dev. 23(9) Pages: 1091-1105
• [Journal Article] An essential role of the universal polarity protein, aPKClambda, on the maintenance of podocyte slit diaphragms. (2009)
  • Author(s): Hirose T, Satoh D, Kurihara H, Kusaka C, Hirose H, Akimoto K, Matsusaka T, Ichikawa I, Noda T, Ohno S
  • Journal Title: PLoS ONE 4(1)
• [Journal Article] The 8th and 9th tandem spectrin-like repeats of utrophin cooperatively form a functional unit to interact with polarity-regulating kinase PAR-1b. (2009)
  • Author(s): Yamashita K, Suzuki a, Satoh Y, Ide M, Amano Y, Masuda-Hirata M, Hayasi Y.K, Hamada K, Ogata K, Ohno S
  • Journal Title: Biochem Biophys Res.Commun 391(1) Pages: 812-817
  • Peer Reviewed
• [Journal Article] A cell polarity protein aPKC1ambda is required for eye lens formation and growth. (2009)
  • Author(s): Sugiyama Y, Akimoto K, Robinson M.L, Ohno S, Qinlan R.A
  • Journal Title: Dev Biol 336(2) Pages: 246-256
  • Peer Reviewed
• [Journal Article] High expression of atypical protein kinase C lambda/iota in gastric cancer as aprognostic factor for recurrence. (2009)
  • Author(s): Takagawa R, Akimoto K, Ichikawa Y, Akiyama H, Kojima Y, Ishiguro H, Inayama Y, Aoki I, Kunisaki C, Endo I, Ngashima Y, Ohno S
  • Journal Title: Ann Surg Oncol 17(1) Pages: 81-88
  • Peer Reviewed
• [Journal Article] aPKClambda/iota promotes growth of prostate cancer cells in an autocrine manner through transcriptional activation of interleukin-6. (2009)
  • Author(s): Ishiguro H, Akimoto K, Nagashima Y, Kojima Y, Sasaki T, Ishiguro-Imagawa Y, Nakaigawa N, Ohno S, Kubota Y, Uemura H
  • Journal Title: Proc Natl Acad Sci USA 106(38) Pages: 16369-16374
  • Peer Reviewed
• [Journal Article] An essential role of the aPKC-Aurora A-NDEL1 pathway in neurite elongationby modulation of microtubule dynamics. (2009)
  • Author(s): Mori D, Yamada M, Mimori-Kiyosue Y, Shirai Y, Suzuki A, Ohno S, Saya H, Wynshaw-Boris A, Hirotsune S
  • Journal Title: Nat Cell Biol 11(9) Pages: 1057-1068
  • Peer Reviewed
• [Journal Article] Interaction between PAR-3 and the aPKC-PAR-6 complex is indispensable for apical domain development of epithelial cells. (2009)
  • Author(s): Horikoshi Y, Suzuki A, Yamanaka T, Sasaki K, Mizuno K, Sawada H, Yonmura S, Ohno S
  • Journal Title: Journal of Cell Science 122(10) Pages: 1595-1606
  • Peer Reviewed
• [Journal Article] SMG-8 and SMG-9, two novel subunits of the SMG-1 complex, regulate remodeling of the mRNA surveillance complex during nonsense-mediated Mrna decay. (2009)
  • Author(s): Yamashita A, Izumi N, Kashima I, Ohnishi T, Saari B, Katsuhata Y, Muramatsu R, Morita T, Iwamatsu A, Hachiya T, Kurata R, Hirano H, Anderson P, Ohno S
  • Journal Title: Genes & Development 23(9) Pages: 1091-1105
  • Peer Reviewed
• [Journal Article] An essential role of the universal polarity protein, aPKC1ambda, on the maintenance of podocyte slit diaphragms. (2009)
  • Author(s): Hirose T, Satoh D, Kurihara H, Kusaka C, Hirose H, Akimoto K, Matsusaka T, Ichikawa I, Noda T, Ohno S
  • Journal Title: PLoS ONE 4(1) Pages: 4194-4194
  • Peer Reviewed
• [Journal Article] Intracellular polarity protein PAR-1 regulates extracellular laminin assembly by regulating the dystroglycan Complex (2009)
  • Author(s): Masuda-Hirata M, Suzuki A, Amano Y, Yamashita K, Ide M, Yamanaka T, Sakai M, Imamura Mand Ohno S
  • Journal Title: Genes to Cells (in press)
  • Peer Reviewed
• [Journal Article] SMG-8 and SMG-9, two novel subunits of the SMG-1 complex, regulate remodeling of the mRNA surveillance complex during nonsense-mediated mRNA decay (2009)
  • Author(s): Yamashita A, Izumi N, Kashima I, Ohnishi T, Saari B, Katsuhata Y, Muramatsu R, Morita T, Iwamatsu A, Hachiya T, Kurata R, Hirano H, Anderson P, Ohno S
  • Journal Title: Genes & Development 23(9) Pages: 1091-1105
  • Peer Reviewed
• [Journal Article] Interaction between PAR-3 and the aPKC-PAR-6 complex is indispensable for apical domain development of epithelial cells (2009)
  • Author(s): Horikoshi Y, Suzuki A, Yamanaka T, Sasaki K, Mizuno K, Sawada H, Yonemura S, Ohno S
  • Journal Title: Journal of Cell Science 122(10) Pages: 1595-1606
  • Peer Reviewed
• [Journal Article] An essential role of the universal polarity protein, aPKClambda, on the maintenance of podocyte slit diaphragms (2009)
  • Author(s): Hirose T, Satoh D, KuriharaH, Kusaka C, Hirose H, Akimoto K, Matsusaka T, Ichikawa I, Noda T, Ohno S
  • Journal Title: PLoS ONE 4(1)
  • Peer Reviewed
• [Journal Article] aPKC enables development of zonula adherens by antagonizing centripetal contraction of the circumferential actomyosin cables. (2008)
  • Author(s): Kishikawa M, Suzuki A, Ohno S
  • Journal Title: J Cell Sci 121(Pt15) Pages: 2481-2492
• [Journal Article] The over- expression and altered localization of the atypical protein kinase C lambda/iota in breast cancer correlates with the pathologic type of these tumors. (2008)
  • Author(s): Kojima Y, Akimoto K, Nagashima Y, Ishiguro H, Shirai S, Chishima T, Ichikawa Y, Ishikawa T, Sasaki T, Kubota Y, Inayama Y, Aoki I, Ohno S, Shimada H
  • Journal Title: Hum Pathol 39(6) Pages: 824-831
• [Journal Article] Role of Lgl/Dlg/Scribble in the regulation of epithelial junction, polarity and growth. (2008)
  • Author(s): Yamanaka T, Ohno S
  • Journal Title: Front Biosci 13 Pages: 6693-6707
• [Journal Article] aPKC restricts the basolateral determinant PtdIns(3, 4, 5)P3 to the basal region. (2008)
  • Author(s): Takahama S, Hirose T, Ohno S
  • Journal Title: Biochem Biophys Res Commun 368(2) Pages: 249-255
• [Journal Article] Rho-kinase phosphorylates PAR-3 and disrupts PAR complex formation. (2008)
  • Author(s): Nakayama M, Goto T. M, Sugimoto M, Nishimura T, Shinagawa T, Ohno S, Amano M, Kaibuchi K
  • Journal Title: Dev Cell 14(2) Pages: 205-215
• [Journal Article] aPKC enables development of zonula adherens by antagonizing centripeta 1 contraction of the circumferential actomyosin cables (2008)
  • Author(s): Kishikawa M, Suzuki A, Ohno S
  • Journal Title: Journal of Cell Science 121(Pt15) Pages: 2481-2492
  • Peer Reviewed
• [Journal Article] The overexpression and altered localization of the atypical protein kinase C lambda/iota in breast cancer correlates with the pathologic type of these tumors (2008)
  • Author(s): Kojima Y, Akimoto K, Nagashima Y, Ishiguro H, Shirai S, Chishima T, Ichikawa Y, Ichikawa T, Sasaki T, Kubota Y, InayamaY, Aoki I, Ohno S, Shimada H
  • Journal Title: Hum Pathol 39(6) Pages: 824-831
  • Peer Reviewed
• [Journal Article] aPKC restricts the basolateral determinant PtdIns(3,4,5)P3 to the basal region (2008)
  • Author(s): Takahama S, Hirose T, Ohno S
  • Journal Title: Biochem. Biophys. Res. Commun 368(2) Pages: 249-255
  • Peer Reviewed
• [Journal Article] .aPKC restricts the basolateral determinant PtdIns(3,4,5)P3 to the basal region (2008)
  • Author(s): Takahama S, Hirose T, Ohno S
  • Journal Title: Biochem.Biophys.Res.Commun 368(2) Pages: 249-255
  • Peer Reviewed
• [Journal Article] Rho-kinase phosphorylates PAR-3 and disrupts PAR complex formation (2008)
  • Author(s): Nakayma M, Goto TM, Sugimoto M, Nishimura T, Shinagawa T, Ohno S, Amano M, Kaibuchi K
  • Journal Title: Dev.Cell 14(2) Pages: 205-215
  • Peer Reviewed
• [Journal Article] Extrinsic Wnt signalling controls the polarity component aPKC. (2007)
  • Author(s): Ohno S
  • Journal Title: Nat. Cell Biol. 9(7) Pages: 738-740
• [Journal Article] Polarity-regulating kinase partitioning- defective 1/microtubule affinity-regulating kinase 2 negatively regulates development of dendrites on hippocampal neurons. (2007)
  • Author(s): Terabayashi T, Itoh T. J, Yamaguchi H, Yoshimura Y, Funato Y, Ohno S, Miki H
  • Journal Title: J Neurosci 27(48) Pages: 13098-107
• [Journal Article] Loss of partitioning-defective-3/isotype -specific interacting protein (par-3/ASIP) in the elongating spermatid of RA175 (IGSF4A/SynCAM)-deficient mice. (2007)
  • Author(s): Fujita E, Tanabe Y, Hirose T, Aurrand-Lions M, Kasahara T, Imhof B. A, Ohno S, Momoi T
  • Journal Title: Am J Pathol 171(6) Pages: 1800-10
• [Journal Article] Helicobacter pylori CagA targets PAR1/MARK kinase to disrupt epithelial cell polarity. (2007)
  • Author(s): Saadat I, Higashi H, Obuse C, Umeda M, Murata-Kamiya N, Saito Y, Lu H, Ohnishi N, Azuma T, Suzuki A, Ohno S, Hatakeyama M
  • Journal Title: Nature 447(7142) Pages: 330-333
• [Journal Article] Distant N- and C-terminal domains are required for intrinsic kinase activity of SMG-1, a critical component of nonsense-mediated mRNA decay. (2007)
  • Author(s): Morita T, Yamashita A, Kashima I, Ogata K, Ishiura S, Ohno S
  • Journal Title: J. Biol. Chem. 282(11) Pages: 7799-7808
• [Journal Article] Neonatal pancreatic cells redifferentiate into both neural and pancreatic lineages. Biochem. (2007)
  • Author(s): Zhao W, Hirose T, Ishikawa M, Oshima Y, Hirai S, Ohno S, Taniguchi H
  • Journal Title: Biophys. Res. Commun. 352(1) Pages: 84-90
• [Journal Article] Polarity-regulating kinase Parlb/MARK2 negatively regulates development of dendrites on hippocampal neurons (2007)
  • Author(s): Terabayshi T, Itoh T.J, Yamaguchi H, Yoshimura Y, Funato Y, Ohno S, Miki H
  • Journal Title: J.Neuroscience 27(48) Pages: 13098-13107
  • Peer Reviewed
• [Journal Article] Loss of Partitiong-defective-3/Isotye-Specific Interacting Protein(Par-3/ASIP)in the Elongating Spermatid of RA175(IGSF4A/SynCAM)Deficieen Mice (2007)
  • Author(s): Fujita E, Tanabe Y, Hirose T, Suzuki A, Aurrand-Lions M, Kasahara T, Imhof B.A, Ohno S, Momoi T
  • Journal Title: AmericanJ.Pathology 171(6) Pages: 1800-1810
  • Peer Reviewed
• [Journal Article] Extrinsic Wnt singalling controls the polarity component aPKC (2007)
  • Author(s): Ohno S
  • Journal Title: Nat.Cell Biol. 9(7) Pages: 738-740
  • Peer Reviewed
• [Journal Article] Helicobacter pylori CagA targets PARI/MARK kinase disrupt epitheila1 ecll polarity (2007)
  • Author(s): Saadat I, Higashi H, Obuse C, Umeda M, Murata-Kamiya N, Saito Y, Lu H, Ohnishi N, Axuma T, Suzuki A, Ohno S, Hatakeyama M
  • Journal Title: Nature 447(7142) Pages: 330-333
  • Peer Reviewed
• [Journal Article] Distant N-and C-terminaldomains are required for intrins kinase activi ty Of SMG-1, a critical component of nonsense-mediated mRNA decay (2007)
  • Author(s): Morita T, Yamashit A, Kashima I, Ogata K, Ishiura S, Ohno S
  • Journal Title: J.Biol, Chem 282(11) Pages: 7799-7808
  • Peer Reviewed
• [Journal Article] Neonatal pancreatic cells redifferentiate into both neural and pancreatic li neages (2007)
  • Author(s): Zhao W, Hirose T, Ishikawa M, Oshima Y, Hirai S, Ohno S, Taniguchi H
  • Journal Title: Bioche, Biophys.Res.Commun 352(1) Pages: 84-90
  • Peer Reviewed
• [Journal Article] JNK activator DLK regulates axon growth and neuronal migration in the developing cerebral cortex. (2006)
  • Author(s): Hirai S-I, Cui1 DF, Miyata T, Ogawa M, Kiyonari H, Suda Y, Aizawa S, Banba Y, Ohno S
  • Journal Title: J. Neuroscience 15,26(46) Pages: 11992-12002
• [Journal Article] Inactivation of aPKCλresults in a loss of adherens junctions in neuroepithelial cells without affecting neurogenesis in mouse neocortex. (2006)
  • Author(s): Imai F, Hirai S-I, Akimoto K, Koyama H, Miyata T, Ogawa M, Noguchi S, Sasaoka T, Noda T, Ohno S
  • Journal Title: Development 133(9) Pages: 1735-1744
• [Journal Article] Lgl mediates apical domain disassembly by suppressing PAR-3/aPKC/PAR-6 complex to orient apical membrane polarity. (2006)
  • Author(s): Yamanaka T, Horikoshi Y, Izumi N, Suzuki A, Mizuno K, Ohno S
  • Journal Title: J. Cell Science 119 Pages: 2107-2118
• [Journal Article] The PAR-aPKC system: lessons in polarity. (2006)
  • Author(s): Suzuki A, Ohno S
  • Journal Title: J Cell Sci 119 Pages: 979-987
• [Journal Article] PAR3 is essential for cyst-mediated epicardial development by establishing apical cortical domains. (2006)
  • Author(s): Hirose T, Karasawa M, Sugitani Y, Fujisawa M, Akimoto K, Ohno S, Noda T
  • Journal Title: Development 133(7) Pages: 1389-1398
• [Journal Article] Binding of a novel SMG-1-Upf1 -eRF1-eRF3 complex (SURF) to the exon junction complex triggers Upf1 phosphory- lation and nonsense-mediated mRNA decay. (2006)
  • Author(s): Kashima I, Yamashita A, Izumi N, Kataoka N, Morishita R, Hoshino S, Ohno M, Dreyfuss G, Ohno S
  • Journal Title: Genes Dev. 20(3) Pages: 355-367
• [Journal Article] Inactivation of aPKC λ results in a loss of adherens junctions in neuroepithelial cells without affecting neurogenesis in mouse neocortex. (2006)
  • Author(s): Imai F, Hirai S-I, Akimoto K, Koyama H, Miyata T, Ogawa M, Noguchi S, Sasaoka T, Noda T, Ohno S
  • Journal Title: Development 133(9) Pages: 1735-1744
• [Journal Article] Lgl mediates apical domain disassembly by suppressing PAR-3/aPKC/PAR-6 complex to orient apical membrane polarity. (2006)
  • Author(s): Yamanaka T, Horikoshi Y, Izumi N, Suzuki A, Mizuno K, Ohno S
  • Journal Title: J.Cell Science 119 Pages: 2107-2118
• [Journal Article] The PAR-aPKC system : lessons in polarity. (2006)
  • Author(s): Suzuki A, Ohno S
  • Journal Title: J.Cell Science 119 Pages: 979-987
• [Journal Article] The {gamma}-Parvin-Integrin-Linked Kinase complex is critically involved in leukocyte-substrate interaction. (2006)
  • Author(s): Yoshimi R, Yamaji S, Suzuki A, Mishima W, Okamura M, Obana T, Matsuda C, Miwa Y, Ohno S, Ishigatsubo Y
  • Journal Title: J.Immunology 176(6) Pages: 13611-13624
• [Journal Article] A distinct PAR complex associates physically with VE-cadherin in vertebrate endothelial cells. (2006)
  • Author(s): Iden S, Rehder D, August B, Suzuki A, Wolburg-Buchholz K, Woburg H, Ohno S, Behrens J, Vestweber D, Klaus E
  • Journal Title: EMBO Rep. 7(12) Pages: 1239-1246
• [Journal Article] Binding of a novel SMG-1-Upf1-eRF1-eRF3 complex (SURF) to the exon junction complex triggers Upflphosphorylation and nonsense-mediated mRNA decay. (2006)
  • Author(s): Kashima I, Yamashita A, Izumi N, Kataoka N, Morishita R, Hoshino S, Ohno M, Dreyfuss G, Ohno S
  • Journal Title: Genes Dev. 20(3) Pages: 355-367
• [Journal Article] An association between the initial SMG-1-Upf1-eRF1-eRF3 complex and exon junction complex on mRNP is required for the SMG-1-mediated phosphorylation of Upf1 during nonsense-mediated mRNA decay. (2006)
  • Author(s): Kashima I, Yamashita A, lzumi N, Kataoka N, Morishita R, Hoshino S, Ohno M, Dreyfuss G, Ohno S
  • Journal Title: Genes Dev. 20(3) Pages: 355-367
• [Journal Article] An association between the initial SMG-1-Upfl-eRF1-eRF3 complex and exon junction complex on mRNP is required for the SMG-1-mediated phosphorylation of Upf1 during nonsense-mediated mRNA decay. (2006)
  • Author(s): Kashima I, Yamashita A, Izumi N, Kataoka N, Morishita R, Hoshino S, Ohno M, Dreyfuss G, Ohno S
  • Journal Title: Genes Dev. 20(3) Pages: 355-367
• [Journal Article] PAR-6-PAR-3 mediates Cdc42-induced Rac activation through the Rac GEFs STEF/Tiam1. (2005)
  • Author(s): Nishimura T, Yamaguchi T, Kato K, Yoshizawa M, Nabeshima Y, Ohno S, Hoshino M, Kaibuchi K
  • Journal Title: Nat. Cell Biol. 7(3) Pages: 270-277
• [Journal Article] The role of SMG-1 on nonsense-mediated mRNA decay. (2005)
  • Author(s): Yamashita A, Kashima I, Ohno S
  • Journal Title: Biochem Biophys.Acta (Proteins and Proteomics), Inhibitors of Protein Kinases 1754(1-2) Pages: 305-315
• [Journal Article] Role of SMG-1-Mediated phosphorylation of Upf1 in NMD. in Nonsense-Mediated mRNA decay. (2005)
  • Author(s): Yamashita A, Kashima I, Ohno S
  • Journal Title: Maquat, Landes Bioscience, (ISBN No.1-58706-296-8) - Pages: 85-95
• [Journal Article] Function of aPKClambda in differentiating photoreceptors is required for proper lamination of mouse retina. (2005)
  • Author(s): Koike C, Nishida A, Akimoto K, Nakaya M-A, Noda T, Ohno S, Furukawa T
  • Journal Title: J.Neurosci. 25(44) Pages: 10290-10298
• [Journal Article] Behavior of tight-junction, adherens-junction and cell polarity proteins during HNF-4alpha-induced epithelial polarization. (2005)
  • Author(s): Satohisa S, Chiba H, Osanai M, Ohno S, Kojima T, Saito T, Sawada N
  • Journal Title: Exp.Cell Res. 310(1) Pages: 66-78
• [Journal Article] Asymmetric distribution of PAR proteins in the mouse embryo begins at the 8-cell stage during compaction. (2005)
  • Author(s): Vinot S, Le T, Ohno S, Pawson T, Maro B, Louvet-Vallee S
  • Journal Title: Dev.Biol. 282(2) Pages: 307-319
• [Journal Article] Expression of MUK/DLK/ZPK, an activator of the JNK pathway, in the nervous systems of the developing mouse embryo. (2005)
  • Author(s): Hirai S-I, Kawaguchi A, Suenaga J, Ono M, Cui de F, Ohno S
  • Journal Title: Gene Expr.Patterns 5(4) Pages: 517-523
• [Journal Article] PAR-6-PAR-3 mediates Cdc42-induced Rac activation through the Rac GEFs STEF/Tiam1. (2005)
  • Author(s): Nishimura T, Yamaguchi T, Kato K, Yoshizawa M, Nabeshima Y, Ohno S, Hoshino M, Kaibuchi K
  • Journal Title: Nat.Cell Biol. 7(3) Pages: 270-277
• [Journal Article] Behavior of tight junction, adherens-junction and cell polarity proteins during HNF-4alpha-induced epithelial polarization. (2005)
  • Author(s): Satohisa S, Chiba H, Osanai M, Ohno S, Kojima T, Saito T, Sawada N
  • Journal Title: Exp.Cell Res. 310(1) Pages: 66-78
• [Journal Article] ASPP2 Regulates Epithelial Cell Polarity through the PAR Complex.
  • Author(s): Cong W, Hirose T, Harita Y, YamashitaA, Mizuno K, Hirano H, Ohno S.
  • Journal Title: Current Biology (in press)
• [Journal Article] Role of Lgl/Dlg/Scribble in the regulation of epithelial junction, polarity and growth
  • Author(s): Yamanaka T, Ohno S
  • Journal Title: Frontiers in Bioscience (in press)
  • Peer Reviewed
• [Journal Article] Symmetrically dividing cell specific division axes alteration obsered in proteasome depleted C.elegans embryo
  • Author(s): Sugiyama Y, Nishimura AOhno S
  • Journal Title: Mechanisms of Development (in press)
  • Peer Reviewed
• [Journal Article] Expression and localisation of apical junctional complex proteins in lens epithelial cells
  • Author(s): Sugiyama Y, Prescott A.R, Tholozan^a F M D, Ohno S, Quinlan R A
  • Journal Title: Experimental Eye Research (in press)
  • Peer Reviewed
• [Journal Article] The overexpression and altered localization of the atypical Protein Kinase C lambda/iota in breast cancer correlates with the pathological type of these tumors
  • Author(s): Kojima Y, Akimoto K, Nagashima Y, Ishiguro H, Shirai S, Chishima T, Ichikawa Y, Insiakama T, Sasaki T, Knbota Y, Knbota Y, Inayama Y, Aoki I, Ohno S, Shimada H
  • Journal Title: Human Pathology (in press)
  • Peer Reviewed
• [Presentation] 細胞シグナリングと疾患モデルのプロテオミクス第7回大会 (2009)
  • Author(s): 大野茂男
  • Organizer: 日本ヒトプロテオーム機構(JHUPO)
  • Place of Presentation: 東京
  • Year and Date: 2009-07-27
• [Presentation] The cell polarity protein, aPKClambda, plays a critical rolein the maintenance of podocyte slit diaphragms, specific type if epithelial intercellular junctions (2008)
  • Author(s): Hirose T, Satoh D, Kurihara H, kusaka C, Hirose H, Akimoto K, Matsusaka T, Ichikawa I, Noda T, Ohno S
  • Organizer: BMB2008
  • Place of Presentation: Kobe
• [Presentation] Conditionally Replicative Adenoviral Vectors for the Treatment of Gastric Cancer (2008)
  • Author(s): Kosaka T, Ono H, Kunisaki C, Julia D, Ohno S, Yamamoto M, Shimada H
  • Organizer: The 67th Annual Meeting of the Japanese Cancer Association
  • Place of Presentation: Nagoya
• [Presentation] The role of aPKC lambda in prostate cancer progression (2008)
  • Author(s): Ishiguro H, Akimoto K, Nagashima Y, Kojima Y, Ishiguro Y, Sasaki T, Umemura H, Ohno S, Kubota Y
  • Organizer: The 67th Annual Meeting of the Japanese Cancer Association
  • Place of Presentation: Nagoya
• [Presentation] Translation Termination Complex During Nonsense-Mediated MRNA Decay (2008)
  • Author(s): Yamashita A, Izumi N, Kashima I, Katsuhata Y, Muramatsu R, Ohno S
  • Organizer: 2008 Cold spring harver labatory meeting on translational control
  • Place of Presentation: New York, USA
• [Presentation] aPKC1 regulates mammary-gland morphogenesis through the transcriptiona 1 suppression of ErbB2/HER-2 gene (2008)
  • Author(s): Akimoto K, Nagashima K, Ishiguro H, Kojima Y, Kim M, Sasaki K, Noda T, Ohno S
  • Organizer: Second JCA-AACR Special Joint Conference, The Latest Advances in Breast Cancer Research : From Basic Science to Therapeutics
  • Place of Presentation: Awaji, Hyogo, Japan
• [Presentation] Possible involvement of PAR-3 in the regulation of slit diaphragm through the direct interaction with Nephrin (2008)
  • Author(s): Satoh D, Hirose T, Kurihara H, Ohno S
  • Organizer: The 7th International Podocyte Conference
  • Place of Presentation: Toronto, Canada
• [Presentation] The cell polarity protein aPKCλ plays a critical role in the maintenance of podocyte slit diaphragms (2008)
  • Author(s): Hirose T, Satoh D, Kurihara H, Akimoto K, Matsusaka T, Ichikawa I, Noda, T, Ohno S
  • Organizer: The 7th International Podocyte Conference
  • Place of Presentation: Toronto, Canada
• [Presentation] The formation of SMG-1 : Upfl : eRF1 : eRF3 complex (SURF) on ribosomes precedes translation-dependent-Upfl phosphorylation during nonsense-medi ated mRNA decay (2008)
  • Author(s): Yamashita A, Kashima I, Izumi N, Katsuhata Y, Muramatsu R, Ohno S
  • Organizer: THE 21st NAITO CONFERENCE"Nuclear Dynamics and RNA[I]"
  • Place of Presentation: Yamanashi, Japan
• [Presentation] Conditional depletion of aPKCλ in eye lens causes microphthalmia and cataract (2008)
  • Author(s): Sugiyama Y, Akimoto K, Robinson M. L, Quinlan, R. A, Ohno S
  • Organizer: ARVO (Association for Research in Vision and Ophthalmology) 2008Annual Meeting, 2008
  • Place of Presentation: Florida USA
• [Presentation] 翻訳依存的なmRNA監視複合体形成および再構成がUPfl リン酸化を誘導する (2007)
  • Author(s): 山下 暁郎, 泉奈 津子, 鹿 勲, 森田 智子, 村松 玲子
  • Organizer: BMB2007-第30回日本分子生物学会年会・第80回日本生化学会大会 合同大会
  • Place of Presentation: 横浜
  • Year and Date: 2007-12-15
• [Presentation] 細胞極性タンパク質、PAR-1bはユーロフィン・ジストログカン複合体制御することを介して細胞外ラミニンの集積を制御している (2007)
  • Author(s): 鈴木 厚、山下 和成、大野 茂男
  • Organizer: BMB2007-第30回日本分子生物学会年会・第80回日本生化学会大会 合同大会
  • Place of Presentation: 横浜
  • Year and Date: 2007-12-11
• [Presentation] Polarity protein PAR-1b regulates extracellular laminin organization through regulation of the utrophin-dystroglycan complex (2007)
  • Author(s): Suzuki A, Amano Y, Yamashita K, Ohno S
  • Organizer: Yokchama Ciyt University 21st Century COEProgram Cell Polarity2007-International Symposium, Cell Polarity System and Future Medicine
  • Place of Presentation: Hayama
  • Year and Date: 2007-12-10
• [Presentation] Defective cell polarity in kidney podocytes causes severe glomerular disease (2007)
  • Author(s): Hirose T, Satoh D, Kuriharawa I, Noda T, Ohno S
  • Organizer: Yokohama City University 21st Century COE Program, Cell Polarity 2007-International symposium on CellPolarity Ststem and Future Medicine
  • Place of Presentation: Hayamn
  • Year and Date: 2007-12-09
• [Presentation] aPKC role in prostatic cancer progression (2007)
  • Author(s): Ishiguro H, Akimoto K, Nagashima Y, Kojima Y, Ishiguro Y, Sasaki T, Uemura H, Ohono S, Kubota Y
  • Organizer: Yokohama City University 21st Century COE Program, Cell Polarity 2007-International symposium on CellPolarity Ststem and Future Medicine
  • Place of Presentation: Hayama
  • Year and Date: 2007-12-09
• [Presentation] The role of apkcl in breast cancer (2007)
  • Author(s): Akimoto K
  • Organizer: Yokohama City University 21st Century COE Program, Cell Polarity 2007-International symposium on CellPolarity Ststem and Future Medicine
  • Place of Presentation: Hayama
  • Year and Date: 2007-12-09
• [Presentation] The role of PAR1/MARK polarity kinase in the interaction between Heli civacter pylori CagA and SHP2 oncoprotein (2007)
  • Author(s): 紙谷 尚子, Iraj Saadat, 東 秀明, 小布施 力史, 梅田 真由美, 齊藤 康弘, Huaischeng Lu, 大西 なおみ, 東 健, 鈴木 厚, 大野 茂男, 畠山 昌則
  • Organizer: 第66回日本癌学会学術総会
  • Place of Presentation: 横浜
  • Year and Date: 2007-10-03
• [Presentation] The role of the PAR-aPKC complex in epithelial morphogenesis and tum origenesis (2007)
  • Author(s): 大野 茂男
  • Organizer: 第66回日本癌学会学術総会
  • Place of Presentation: 横浜
  • Year and Date: 2007-10-03
• [Presentation] The SMG-1:SMG-8:p60 Complex Regulated Phosphorylation Of Upf1Is Repuired For The Dissociation Of eRFs From mRNA Surveillance Complex During Nonsense-mediated mRNA decay (2007)
  • Author(s): Yamashita A, Izumi N, Kashima I, Ohnishi T, Amderson P, Ohno S.
  • Organizer: 第9回日本RNA学会年会
  • Place of Presentation: 名古屋
  • Year and Date: 2007-07-28
• [Presentation] The SMG-1:SMG-8:p60 Complex Regulated Phosphorylation OF Upf1 Is Required For The Dissociation Of eRFs From mRNA Surveillance Complex During Nonsense-mediated mRNA decay (2007)
  • Author(s): Yamashita A, Kashima I, Izumi N, Ohnishi T, Anderson P, Ohno S.
  • Organizer: 第12回国際RNA学会年会
  • Place of Presentation: University of Wisconsin,Madison
  • Year and Date: 2007-06-03
• [Presentation] A novel signaling pathway by which PAR-aPKC system regulates cell-substate interaction (2007)
  • Author(s): 鈴木 厚、山下 和成、天野 芳子、大野 茂男
  • Organizer: 第40回日本発生生物学会, 第59回細胞生物学会合同大会シンポジウム
  • Place of Presentation: 福岡
  • Year and Date: 2007-05-30
• [Presentation] mRNAの品質鹽機構〜分子機構とその振作技術の物学・医学への展開〜 (2007)
  • Author(s): 大野 茂男
  • Organizer: 日本生化学北陸支部会
  • Place of Presentation: 金沢
  • Year and Date: 2007-05-26
• [Book] 生化学・第2版 (2007)
  • Author(s): 大野 茂男
  • Total Pages: 217
  • Publisher: 東京化学同人
• [Remarks] 新聞報道
• [Remarks] 2010.4.13 経産業新聞、DNA損傷防ぐ仕組み解明
• [Remarks] 2009.10.20 日経産業新聞、前立腺がん再発関連の遺伝子発見
• [Remarks] 2009.2.22 読売新聞、腎糸球体の血液ろ過膜機能維持に新たな機構
• [Remarks] ホームページ
  • URL: http://www-user.yokohama-cu.ac.jp/~ohnos//Japanease/indexJ.html
• [Remarks]
  • URL: http://www-user.yokohama-cu.ac.jp/~ohnos/Japanease/indexJ.html
• [Remarks]
  • URL: http://www-user.yokohama-cu.acjp/?ohnos//Japanease/indexihtnal
• [Remarks]
  • URL: http://www-user.yokohama-cu.ac.jp/~ohnos/Japanease/indexJ.html
• [Patent(Industrial Property Rights)] 初期乳がんモデル用動物 (2009)
  • Inventor(s): 秋本和憲、大野茂男
  • Industrial Property Rights Holder: 公立大学法人横浜市立大学
  • Filing Date: 2009-06-18
• [Patent(Industrial Property Rights)] 初期乳がんモデル用動物 (2007)
  • Inventor(s): 秋本和憲、野田哲生、大野茂男
  • Industrial Property Rights Holder: 横浜市立大学
  • Industrial Property Number: 2007-167036
  • Filing Date: 2007-06-25
• [Patent(Industrial Property Rights)] mRNAサーベイランス経路を制御するタンパク質 (2007)
  • Inventor(s): 山下暁朗、大野茂男
  • Industrial Property Rights Holder: 横浜市立大学
  • Industrial Property Number: 2007-179140
  • Filing Date: 2007-07-08