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Development of new types of retrovirus vectors that modulate epigenetical regulation.

Research Project

Project/Area Number 17016015
Research Category

Grant-in-Aid for Scientific Research on Priority Areas

Allocation TypeSingle-year Grants
Review Section Biological Sciences
Research InstitutionThe University of Tokyo

Principal Investigator

IBA Hideo  The University of Tokyo, 医科学研究所, 教授 (60111449)

Co-Investigator(Kenkyū-buntansha) HARAGUCHI Takeshi  東京大学, 医科学研究所, 助教 (10549455)
MIZUTANI Taketoshi  東京大学, 医科学研究所, 助教 (00376617)
MINOGUCHI Shigeru  東京大学, 医科学研究所, 助教 (60322757)
YAMAMICHI Nobutake  東京大学, 医学部附属病院, 非常勤医員 (30463897)
ITO Taiji  東京大学, 医科学研究所, 助手 (60343109)
Project Period (FY) 2005 – 2009
Project Status Completed (Fiscal Year 2009)
Budget Amount *help
¥46,200,000 (Direct Cost: ¥46,200,000)
Fiscal Year 2009: ¥9,300,000 (Direct Cost: ¥9,300,000)
Fiscal Year 2008: ¥9,300,000 (Direct Cost: ¥9,300,000)
Fiscal Year 2007: ¥9,300,000 (Direct Cost: ¥9,300,000)
Fiscal Year 2006: ¥9,300,000 (Direct Cost: ¥9,300,000)
Fiscal Year 2005: ¥9,000,000 (Direct Cost: ¥9,000,000)
Keywordsレトロウイルスベクター / RelB / requiem / 足場非依存的な増殖 / Brm / NFkappaB / SWI / SNFクロマチン構造変換因子 / miRNA / SNF複合体 / NFkB / AP-1 / Requiem / Decoy RNA / レンチウイルスベクター / microRNA / プロモーター / NF-I / RNA干渉 / 大腸癌 / feedback 制御 / in situ hybridization / レトロウイルス / shRNA / miR-21 / NFI-B / レンチウイルス / ウイルスベクター / 遺伝子治療 / GFP遺伝子 / Brm遺伝子 / siRNA
Research Abstract

In this project, we have designed, prepared and improved several retrovirus/lentivirus vectors that carry efficient transcriptional units for the expression of short hairpin (sh) RNA, miRNA, and the newly developed RNA decoy molecule that inhibits specific miRNA (TuD RNA). Using these vector systems, we have studied cancer epigenetics by concentrating on SWI/SNF chromatin remodeling complex.
We have noticed that in human tumor cell lines that are deficient in Brm expression, expression of MLV and HIV vectors that were exogenously transduced into them are rapidly silenced stochastically. We finally demonstrated that Brm-type SWI/SNF complex is essential for the stable expression of retro/lentivirus.
Brm is further shown to have antioncogenic potential because exogenous introduction of Brm reduces oncogenic potential of cell lines deficient in Brm expression. In all these cell lines examined, the functional Brm gene is present and is actively transcribed; Brm expression was suppressed at t … More he post-transcriptional level. We hypothesized that Brm is targeted by certain miRNAs and screened several miRNA candidates and finally have shown that miR-199a target Brm mRNA. Interestingly, all the cancer cell lines deficient in Brm have high levels of miR-199a, whereas in Brm expressing cells, miR-199a was marginally expressed.
We further show these distinct expression patterns are resulted from double-negativefeedback regulation formed between Brm and miR-199a-5p/-3p via a transcription factor Egr1. We additionally have shown that miR-21 and its target NFIB, a negative transcriptional regulator, also forms a robust feedback regulation in cancer cell lines.
We have also shown human requiem protein functions as an adaptor protein that links SWI/SNF complex and RelB/p52. We further showed that introduction of shRNA against REQ strongly suppresses anchourage-independent growth, but does not affect growth in monolayer culture at all in tumor cell lines such as Panc-1, in which non-canonical NFκB pathway is constitutively activated. Less

Report

(6 results)
  • 2009 Annual Research Report   Final Research Report ( PDF )
  • 2008 Annual Research Report
  • 2007 Annual Research Report
  • 2006 Annual Research Report
  • 2005 Annual Research Report
  • Research Products

    (94 results)

All 2009 2008 2007 2006 2005 Other

All Journal Article (46 results) (of which Peer Reviewed: 22 results) Presentation (45 results) Remarks (3 results)

  • [Journal Article] Loss of the Brm-type SWI/SNF chromatin remodeling complex is a strong barrier to the Tat-independent transcriptional elongation of HIV-1 transcripts.2009

    • Author(s)
      Mizutani T, Ishizaka A, Tomizawa M, Okazaki T, Yamamichi N, Kawana-Tachikawa A, Iwamoto A, Iba H.
    • Journal Title

      J of Virol. 83

      Pages: 11569-11580

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] LNA in situ hybridization analysis of miR-21 expression during colorectal cancer development.2009

    • Author(s)
      Yamamichi N, Shimomura R, Inada K, Sakurai K, Haraguchi T, Ozaki Y, Fujita S, Mizutani T, Furukawa C, Fujishiro M, Ichinose M, Shiogama K, Tsutsumi Y, Omata M, Iba H.
    • Journal Title

      Clin. Cancer Res. 15

      Pages: 4009-4016

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Cdx2 and the Brm-type SWI/SNF complex cooperatively regulate villin expression in gastrointestinal cells.2009

    • Author(s)
      Yamamichi N, Inada K, Furukawa C, Sakurai K, Tando T, Ishizaka A, Haraguchi T, Mizutani T, Fujishiro M, Shimomura R, Oka M, Ichinose M, Tsutsumi Y, Omata M, Iba H.
    • Journal Title

      Exp. Cell Res. 315

      Pages: 1779-1789

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Vectors expressing efficient RNA decoys achieve the long-term suppression of specific microRNA activity in mammalian cells.2009

    • Author(s)
      Haraguchi T, Ozaki Y, Iba H.
    • Journal Title

      Nucleic Acids Res. 37

    • Related Report
      2009 Final Research Report
  • [Journal Article] Loss of the Brm-type SWI/SNF chromatin remodeling complex is a strong barrier to the Tat-independent transcriptional elongation of HIV-1 transcripts2009

    • Author(s)
      Mizutani, T., Ishizaka, A., Tomizawa, M., Okazaki, T., Yamamichi, N., Kawana-Tachikawa, A., Iwamoto, A., Iba, H.
    • Journal Title

      Journal of Virology 83

      Pages: 11569-11580

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] LNA in situ hybridization analysis of miR-21 expression during colorectal cancer development2009

    • Author(s)
      Yamamichi, N., Shimomura, R., Inada, K., Sakurai, K., Haraguchi, T., Ozaki, Y., Fujita, S., Mizutani, T., Furukawa, C., Fujishiro, M., Ichinose, M., Shiogama, K., Tsutsumi, Y., Omata, M., Iba, H
    • Journal Title

      Clinical Cancer Research 15

      Pages: 4009-4016

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Cdx2 and the Brm-type SWI/SNF complex cooperatively regulate villin expression in gastrointestinal cells2009

    • Author(s)
      Yamamichi, N., Inada, K., Furukawa, C., Sakurai, K., Tando, T., Ishizaka, A., Haraguchi, T., Mizutani, T., Fujishiro, M., Shimomura, R., Oka, M., Ichinose, M., Tsutsumi, Y., Omata, M., Iab, H.
    • Journal Title

      Experimental Cell Research 315

      Pages: 1779-1789

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] miRNAの活性抑制法とその利用によるヒトがん治療「miRNAが変える創薬・がん・発生研究-次世代高感度アレイによる統合解析が明らかにするmiRNAの生命機能」2009

    • Author(s)
      櫻井浩平、古川千尋、原口健、伊庭英夫
    • Journal Title

      分子細胞治療 8

      Pages: 350-357

    • Related Report
      2009 Annual Research Report
  • [Journal Article] がん研究の巨人"Saburo"逝く-花房秀三郎博士の歩み-2009

    • Author(s)
      伊庭英夫
    • Journal Title

      現代化学 6月号

      Pages: 37-39

    • Related Report
      2009 Annual Research Report
  • [Journal Article] miR-21の発現亢進と発癌-亢進は癌の原因か、結果か? またいかに亢進抑制するのか?2009

    • Author(s)
      原口健、伊庭英夫
    • Journal Title

      実験医学 27

      Pages: 1201-1207

    • Related Report
      2009 Annual Research Report
  • [Journal Article] miRNAと転写制御因子が形成するネットワーク2009

    • Author(s)
      伊庭英夫
    • Journal Title

      ゲノム医学 9

      Pages: 23-29

    • Related Report
      2009 Annual Research Report
  • [Journal Article] Mir-21 gene expression triggered by AP-1 is sustained through a double negative feedback mechanism.2008

    • Author(s)
      Fujita S, Ito T, Mizutani T, Minoguchi S, Yamamichi N, Sakurai K, Iba, H.
    • Journal Title

      J. Mol. Biol 378

      Pages: 492-504

    • Related Report
      2009 Final Research Report
  • [Journal Article] Instability of retroviral DNA methylation in embryonic stem cells.2008

    • Author(s)
      Minoguchi S, Iba, H.
    • Journal Title

      Stem Cells 26

      Pages: 1166-1173

    • Related Report
      2009 Final Research Report
  • [Journal Article] Class-specific regulation of pro-inflammatory genes by MYD88 pathways and IκBζ.2008

    • Author(s)
      Kayama H, Ramirez-Carrozzi VR, Yamamoto M, Mizutani T, Kuwata H, Iba H., Matsumoto M, Honda, K, Smale ST, Takeda, K.
    • Journal Title

      J. Biol. Chem. 283

      Pages: 12468-12477

    • Related Report
      2009 Final Research Report
  • [Journal Article] Brm transactivates the telomerase reverse transcriptase (TERT) gene and modulates the splicing patterns of its transcripts in concert with p54nrb2008

    • Author(s)
      Ito T, Watanabe H, Yamamichi N, Kondo S, Tando T, Haraguchi T, Mizutani T, Sakurai K, Fujita S, Izumi T, Isobe T, Iba H.
    • Journal Title

      Biochemical J. 411

      Pages: 201-209

    • Related Report
      2009 Final Research Report
  • [Journal Article] Putative promoter regions of miRNA genes involved in evolutionarily conserved regulatory systems among vertebrates.2008

    • Author(s)
      Fujita S, Iba H.
    • Journal Title

      Bioinformatics 24

      Pages: 303-308

    • Related Report
      2009 Final Research Report
  • [Journal Article] Mir-21 gene expression triggered by AP-1 is sustained through a double negative feedback mechanism2008

    • Author(s)
      Fujita, S., Ito, T., Mizutani, T., Minoguchi, S., Yamarnichi, N., Sakurai, K., and Iba, H.
    • Journal Title

      J.Mol.Biol. 378

      Pages: 492-504

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Instability of retroviral DNA methylation in embryonic stem cells2008

    • Author(s)
      Minoguchi,S. and Iba, H.
    • Journal Title

      Stein Cells 26

      Pages: 1166-1173

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Class-specific regulation of pro-inflammatory genes by MYD88 pathways and IκBζ2008

    • Author(s)
      Kayama, H., Ramirez-Carrozzi, V.R., Yamamoto, M., Mizutani, T., Kuwata, H., Iba, H., Matsumoto, M., Honda, K., Smale, S.T., and Takeda, K.
    • Journal Title

      J. Biol. Chem. 283

      Pages: 12468-12477

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Brm transactivates the telomerase reverse transcriptase (TERT) gene and modulates the splicing patterns of its transcripts in concert with p54^<nrb>2008

    • Author(s)
      Ito, T., Watanabe, H., Yamamichi, N., Kondo, S. , Tando, T. ,H araguchi, T., Mizutani, T., Sakurai, K., Fujita, S., Izumi, T., Isobe, T., and Iba, H.
    • Journal Title

      Biochemical J. 411

      Pages: 201-209

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Putative promoter regions of miRNA genes involved in evolutionarily conserved regulatory systems among vertebrates2008

    • Author(s)
      Fujita, S., and Iba, H.
    • Journal Title

      Bioinformatics 24

      Pages: 303-308

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] miRNAの遺伝子制御ネットワーク2008

    • Author(s)
      藤田修二、伊庭英夫
    • Journal Title

      蛋白質核酸酵素 53

      Pages: 1781-1786

    • Related Report
      2008 Annual Research Report
  • [Journal Article] クロマチン構造変換因子と癌-Brm型SWI/SNF複合体と胃癌2008

    • Author(s)
      山道信毅、伊庭英夫
    • Journal Title

      臨床検査 52

      Pages: 693-697

    • Related Report
      2008 Annual Research Report
  • [Journal Article] Mir-21 gene expression triggered by AP-1 is sustained through a double negative feedback mechanism.2008

    • Author(s)
      Fujita, S., Ito, T., Mizutani, T., Minoguchi, S., Yamamichi, N., Sakurai, K. and Iba, H.
    • Journal Title

      J. Mol. Biol. (In press)

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Instability of retroviral DNA methylation in embryonic stem cells.2008

    • Author(s)
      Minoguchi, S. and Iba, H.
    • Journal Title

      Stem Cells (In press)

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Class-specific regulation of pro-inflammatory genes by MYD88 pathways and lκBζ.2008

    • Author(s)
      Kayama, H., Ramirez-Carrozzi, V. R, Yamamoto, M., Mizutani, T., Kuwata, H., Iba, H., Matsumoto, M., Honda, K., Smale, S.T., and Takeda, K.
    • Journal Title

      J. Biol. Chem. (In press)

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Brm transactivates the telomerase reverse transcriptase (TERT) gene and modulates the splicing patterns of its transcripts inconcert with p54^<nrb>.2008

    • Author(s)
      Ito, T., Watanabe, H., Yamamichi, N., Kondo, S., Tando, T., Haraguchi, T., Mizutani, T., Sakurai, K., Fujita, S., Izumi, T., Isobe, T., and Iba, H.
    • Journal Title

      Biochemical J. 411

      Pages: 201-209

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Putative promoter regions of miRNA genes involved in evolutionarily conserved regulatory systems among vertebrates.2008

    • Author(s)
      Fujita, S. and Iba, H.
    • Journal Title

      Bioinformatics 24

      Pages: 303-308

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] SiRNAs do not induce RNA-dependent transcriptional silencing of retrovirus in human cells.2007

    • Author(s)
      Haraguchi T, Mizutani T, Yamamichi N, Ito T, Minoguchi S, Iba, H.
    • Journal Title

      FEBS Letter 581

      Pages: 4949-4954

    • Related Report
      2009 Final Research Report
  • [Journal Article] Frequent loss of Brm expression in gastric cancer correlates with histological features and differentiation state.2007

    • Author(s)
      Haraguchi T, Fujita S, Tsutsumi, Y, Omata M, Iba, H.
    • Journal Title

      Cancer Research 67

      Pages: 10727-10735

    • Related Report
      2009 Final Research Report
  • [Journal Article] RNA-dependent transcriptional silencing does not contribute to retroviral gene silencing in human cells.2007

    • Author(s)
      Haraguchi, T., Mizutani, T., Yamamichi, N., Ito, T., Minoguchi, S., and Iba, H.
    • Journal Title

      FEBS Letter 581

      Pages: 4949-4954

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Frequent loss of Brm expression in gastric cancer correlates with histological features and differentiation state.2007

    • Author(s)
      Yamamichi, N., Inada, K., Ichinose, M., Yamamichi-Nishina, M., Mizutani, T., Watanabe, H., Shiogama, K., Okazaki, T., Fujishiro, M., Yahagi, N., Haraguchi T., Fujita, S., Tsutsumi, Y., Omata, M. and Iba, H.
    • Journal Title

      Cancer Research 22

      Pages: 10727-10735

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Behavior of transplanted bone marrow derived mesenchymal stem cells in periodontal defects.2006

    • Author(s)
      Hasegawa N, Kawaguchi H, Hirachi A, Takeda K, Mizuno N, Nishimura M, Koike C, Tsuji K, Iba, H, Kato Y, Kurihara, H.
    • Journal Title

      J. Periodontol. 77

      Pages: 1003-7

    • Related Report
      2009 Final Research Report
  • [Journal Article] A protein associated with Toll-like Receptor 4 (PRAT4A) regulates cell surface expression of TLR4.2006

    • Author(s)
      Wakabayashi Y, Kobayashi M, Akashi-Takamura S, Tanimura N, Konno K, Takahashi K, Ishii T, Mizutani T, Iba, H, Kouro T, Takaki S, Takatsu K., Oda, Y., Ishihama, Y., Saitoh, S -I., Miyake, K.
    • Journal Title

      J. Immunol 177

      Pages: 1772-1779

    • Related Report
      2009 Final Research Report
  • [Journal Article] SWI/SNF complex is essential for NRSF-mediated suppression of neuronal genes in human nonsmall cell lung carcinoma cell lines.2006

    • Author(s)
      Watanabe H, Mizutani T, Haraguchi T, Yamamichi N, Minoguchi S, Yamamichi-Nishina M, Mori N, Kameda T, Sugiyama T, Iba H.
    • Journal Title

      Oncogene 25

      Pages: 470-479

    • Related Report
      2009 Final Research Report
  • [Journal Article] SWI/SNF complex is essential for NRSF-mediated suppression of neuronal genes in human nonsmall cell lung carcinoma cell lines.2006

    • Author(s)
      Watanabe H. et al.
    • Journal Title

      Oncogene 25

      Pages: 470-479

    • Related Report
      2006 Annual Research Report
  • [Journal Article] A protein associated with toll-like receptor 4 (PRAT4A) regulates cell surface expression of TLR4.2006

    • Author(s)
      Wakabayashi Y. et al.
    • Journal Title

      Journal of Immunol 177

      Pages: 1772-1779

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Behavior of transplanted bone marrow derived mesenchymal stem cells in periodontal defects.2006

    • Author(s)
      Hasegawa N. et al.
    • Journal Title

      Journal of Periodontology 77

      Pages: 1003-1007

    • Related Report
      2006 Annual Research Report
  • [Journal Article] SWI/SNF complex is essential for NRSF-mediated suppression of neuronal genes in human nonsmall cell lung carcinoma cell lines2006

    • Author(s)
      Watanabe, H. et al.
    • Journal Title

      Oncogene 25

      Pages: 470-479

    • Related Report
      2005 Annual Research Report
  • [Journal Article] The Brm gene suppressed at the post-transcriptional level in various human cell lines is inducible by transient HDAC inhibitor treatment, which exhibits anti-oncogenic potential.2005

    • Author(s)
      Yamamichi N, Yamamichi-Nishina M, Mizutani T, Watanabe H, Minoguchi S, Kobayashi N, Kimura S, Ito T, Yahagi N, Ichinose M, Omata M, Iba, H.
    • Journal Title

      Oncogene 24

      Pages: 5471-5481

    • Related Report
      2009 Final Research Report
  • [Journal Article] The Brm gene suppressed at the post-transcriptional level in various human cellines is inducible by transient HDAC inhibitor treatment, which exhibits antioncogenic potential2005

    • Author(s)
      Yamamichi, N. et al.
    • Journal Title

      Oncogene 24

      Pages: 5471-5481

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Requiem protein links RelB/p52 and the Brm-type SWI/SNF complex in a non-canonical NFκB pathway.

    • Author(s)
      Tando T, Ishizaka A, Watanabe H, Ito T, Iida S, Haraguchi T, Mizutani T, Izumi T, Isobe T, Akiyama T, Inoue J, Iba H.
    • Journal Title

      J Biol. Chem. (in press)

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article]

    • Author(s)
      Yamamichi N, Inada K, Ichinose M, Yamamichi-Nishina M, Mizutani T, Watanabe, H., Shiogama K, Okazaki T, Fujishiro M, Yahagi N
    • Related Report
      2009 Final Research Report
  • [Journal Article] Role of glucocorticoid receptor in the regulation of cellular sensitivity to irinotecan hydrochloride

    • Author(s)
      Akagi, T., Fukagawa, T., Kage, Y., To, H. , Matsunaga, N. , Koyanagi, S. , Uchida, A., Fujii, A., Iba, H., Ikemura, T., Aramaki, H., Higuchi, S., and Ohdo.S.
    • Journal Title

      J. Pharmacol. Sci. (in press)

    • NAID

      10025734592

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Ccbc2 and the Brm-type SWI/SNF complex cooperatively regulate villin expression in gastrointestinal cells

    • Author(s)
      Yamamichi, N., Inada, K., Furukawa, C., Sakurai, K., Tando, T. , Ishizaka , A., Haraguchi, T., Mizutani, T., Fujishiro, M., Shimomura, R., Oka, M., Ichinose, M., Tsutsumi, Y., Ornata, M., and Iba, H.
    • Journal Title

      Exp. Cell Res. (in press)

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Vectors expressing efficient RNA decoys achieve the long-term suppression of specific microRNA activity in mammalian cells

    • Author(s)
      Haraguchi, T., Ozaki, Y., and Iba, H.
    • Journal Title

      Nucleic Acids Res. (in press)

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Presentation] Requiem functions in anon-canonical NFκB pathway as a linker protein between Rel/p52 and the SWI/SNF complex2009

    • Author(s)
      丹藤利夫、石坂彩、原口健、水谷壮利、秋山泰身、井上純一郎、伊庭英夫
    • Organizer
      第32回日本分子生物学会年会
    • Place of Presentation
      横浜
    • Year and Date
      2009-12-09
    • Related Report
      2009 Final Research Report
  • [Presentation] 特定のmicroRNAを高効率で阻害するDecoy RNAの設計(Design of efficient RNA decoys that inhibit specific microRNA activity in mammalian cells. )2009

    • Author(s)
      原口健、伊庭英夫
    • Organizer
      第32回日本分子生物学会年会
    • Place of Presentation
      横浜
    • Year and Date
      2009-12-09
    • Related Report
      2009 Final Research Report
  • [Presentation] Requiem functions in a non-canonical NFκB pathway as a linker protein between Rel/p52 and the SWI/SNF complex2009

    • Author(s)
      丹藤利夫、石坂彩、原口健、水谷壮利、秋山泰身、井上純一郎、伊庭英夫
    • Organizer
      第32回日本分子生物学会年会
    • Place of Presentation
      横浜
    • Year and Date
      2009-12-09
    • Related Report
      2009 Annual Research Report
  • [Presentation] 特定のmicroRNAを高効率で阻害する Decoy RNAの設計(Design of efficient RNA decoys that inhibit specific microRNA activity in mammalian cells)2009

    • Author(s)
      原口健、伊庭英夫
    • Organizer
      第32回日本分子生物学会年会
    • Place of Presentation
      横浜
    • Year and Date
      2009-12-09
    • Related Report
      2009 Annual Research Report
  • [Presentation] Synthesis of 5'-capped sirna for suppression of off-target effects and its capability to achieveprotein expression2009

    • Author(s)
      Ogata, A., Ueno, Y., Furukawa, C., Sakurai, K., Iba, H., Kitamura, Y., Kitade, Y.
    • Organizer
      Joint Symposium of 5th Annual Meeting of Oligonucleotide TherapeuticsSociety and the 19th Antisense Symposium
    • Place of Presentation
      福岡
    • Year and Date
      2009-11-03
    • Related Report
      2009 Final Research Report
  • [Presentation] Synthesis of 5'-capped sirna for suppression of off-target effects and its capability to achieve Protein expression2009

    • Author(s)
      Ogata, A., Ueno, Y., Furukawa, C., Sakurai, K., Iba, H., Kitamura, Y., Kitade, Y.
    • Organizer
      Joint Symposium of 5^<th> Annual Meeting of Oligonucleotide Therapeutics Society and the 19^<th> Antisense Symposium
    • Place of Presentation
      福岡
    • Year and Date
      2009-11-03
    • Related Report
      2009 Annual Research Report
  • [Presentation] レトロウイルスと宿主細胞間の相互作用のエピジェネティクス-ニワトリレトロウイルス研究の魅力とその展開2009

    • Author(s)
      伊庭英夫
    • Organizer
      第57回日本ウイルス学会学術集会
    • Place of Presentation
      東京
    • Year and Date
      2009-10-26
    • Related Report
      2009 Final Research Report
  • [Presentation] HIV-1 Tat非依存的な発現におけるSWI/SNF複合体の作用点の解析2009

    • Author(s)
      水谷壮利
    • Organizer
      第57回日本ウイルス学会学術集会
    • Place of Presentation
      東京
    • Year and Date
      2009-10-26
    • Related Report
      2009 Final Research Report
  • [Presentation] EBウイルス感染を制御する宿主転写因子の同定2009

    • Author(s)
      浅井理沙、丹藤利夫、伊庭英夫、神田輝、高田賢蔵、上間匡、川口寧
    • Organizer
      第57回日本ウイルス学会学術集会
    • Place of Presentation
      東京
    • Year and Date
      2009-10-26
    • Related Report
      2009 Final Research Report
  • [Presentation] クロマチン構造変換因子SWI/SNF複合体の胃癌における発現異常2009

    • Author(s)
      山道信毅、稲田健一、一瀬雅夫、藤城光弘、小俣政男、伊庭英夫
    • Organizer
      触媒サブユニットBrmの発現欠失、組織型・分化マーカーとの関係消化器病学会
    • Place of Presentation
      京都
    • Year and Date
      2009-10-14
    • Related Report
      2009 Final Research Report
  • [Presentation] Gene regulatory networks formed between miR-21 and its targets during the course of carcinogenesis2009

    • Author(s)
      Iba, H.
    • Organizer
      第8回日中がんワークショップ
    • Place of Presentation
      大阪
    • Year and Date
      2009-10-05
    • Related Report
      2009 Annual Research Report 2009 Final Research Report
  • [Presentation] The Brm-type SWI/SNF chromatin remodeling complex plays an important role in transcriptional elongation of HIV-1 in the absence of Tat2009

    • Author(s)
      Ishizaka, A., Mizutani, T., Tomizawa, M., Kawana-Tachikawa, A., Iwamoto, A., Iba, H.
    • Organizer
      The 9thAwaji International Forum on Infection and Immunity
    • Place of Presentation
      淡路島
    • Year and Date
      2009-09-08
    • Related Report
      2009 Final Research Report
  • [Presentation] The Brin-type SWI/SNF chromatin remodeling complex plays an important role in transcriptional elongation of HIV-1 in the absence of Tat2009

    • Author(s)
      Ishizaka, A., Mizutani, T., Tomizawa, M., Kawana-Tachikawa, A., Iwamoto, A., Iba, H.
    • Organizer
      The 9^<th> Awaji International Forum on Infection and Immunity
    • Place of Presentation
      淡路島
    • Year and Date
      2009-09-08
    • Related Report
      2009 Annual Research Report
  • [Presentation] microRNAの異常発現と発がん2009

    • Author(s)
      伊庭英夫
    • Organizer
      第41回ケムバイオシンポジウム次世代医療:microRNAとcancer stem cells
    • Place of Presentation
      東京工業大学大岡山キャンパス
    • Year and Date
      2009-03-05
    • Related Report
      2009 Final Research Report
  • [Presentation] 特定のmicroRNAを長期間、高効率で阻害するDecoy RNA発現ベクター2009

    • Author(s)
      原口健、伊庭英夫
    • Organizer
      第68回日本癌学会学術総会
    • Place of Presentation
      横浜
    • Related Report
      2009 Final Research Report
  • [Presentation] A Catalytic Subunit of the SWI/SNF Complex is Required for Tat-independent Stable Expression of HIV-1 1st China-Japan2008

    • Author(s)
      A Catalytic Subunit of the SWI/SNF Complex is Required for Tat-independent Stable Expression of HIV-1 1st China-Japan
    • Organizer
      Joint Workshop on New-Generation Vaccines for Infectious Diseases
    • Place of Presentation
      北京
    • Year and Date
      2008-05-22
    • Related Report
      2009 Final Research Report
  • [Presentation] Brm, a catalytic subunit of the SWI/SNF complex is required for Tat-independent stable expression of HIV-12008

    • Author(s)
      Mizutani, T., Ishizaka, A., Tomizawa, M., Yamamichi, N., Tando, T., Iwamoto, A., and Iba, H.
    • Organizer
      China-Japan ajoint Workshop on New-Generation Vaccines for Infectious Diseases
    • Place of Presentation
      北京
    • Year and Date
      2008-05-22
    • Related Report
      2008 Annual Research Report
  • [Presentation] miR-21と標的遺伝子の発現制御ネットワーク解析(Analysis of regulatory network involving human miR-21 gene and its target genes. )2008

    • Author(s)
      藤田修二、山道信毅、原口健、尾崎由佳、伊庭英夫
    • Organizer
      第31回日本分子生物学会年会第81回日本生化学会大会合同大会
    • Place of Presentation
      神戸
    • Related Report
      2009 Final Research Report
  • [Presentation] ヒトvillin遺伝子の転写制御機構の解析2008

    • Author(s)
      古川千尋、山道信毅、丹藤利夫、石坂彩、櫻井浩平、原口健、稲田健一、下村龍一、伊庭英夫
    • Organizer
      第31回日本分子生物学会年会第81回日本生化学会大会合同大会
    • Place of Presentation
      神戸
    • Related Report
      2009 Final Research Report
  • [Presentation] miRNAが形成する制御ネットワークとがん2008

    • Author(s)
      伊庭英夫、藤田修二、山道信毅、櫻井浩平、水谷壮利
    • Organizer
      第67回日本癌学会学術総会
    • Place of Presentation
      名古屋
    • Related Report
      2009 Final Research Report
  • [Presentation] HIV-1のTat非依存的な発現を維持するSWI/SNF複合体の作用機序の解析2008

    • Author(s)
      石坂彩、水谷壮利、山道信毅、原口健、富沢麻利子、岩本愛吉、伊庭英夫
    • Organizer
      第5回日本ウイルス学会学術集会
    • Place of Presentation
      岡山
    • Related Report
      2009 Final Research Report
  • [Presentation] AP-1 triggers the sustained transcription of miR-21 through an evolutionarily conserved feedback mechanism2007

    • Author(s)
      Fujita S, Ito T, Mizutani T, Minoguchi S, Yamamichi N, Sakurai K, Iba H.
    • Organizer
      The 11th German-Japanese Cancer Workshop
    • Place of Presentation
      京都
    • Year and Date
      2007-11-30
    • Related Report
      2009 Final Research Report 2007 Annual Research Report
  • [Presentation] Frequent Loss of Brm Expression in Gastric Cancer Correlates with Histological Features and Differentiation State2007

    • Author(s)
      Yamamichi N, Inada K, Iba H.
    • Organizer
      第14回The East Asian Joint Symposium (EAJS14): Expanding Scientific Extending Therapeutic Horizons
    • Place of Presentation
      東京
    • Year and Date
      2007-11-13
    • Related Report
      2009 Final Research Report 2007 Annual Research Report
  • [Presentation] High expression of miR-21 was detected in colorectal carcinoma but not in adenoma by LNA-based in situhybridization2007

    • Author(s)
      山道信毅、下村龍一、水谷壮利、稲田健一、藤田修二、櫻井浩平、一瀬雅夫、堤寛、小俣政男、伊庭英夫
    • Organizer
      第66回日本癌学会総会
    • Place of Presentation
      横浜
    • Year and Date
      2007-10-05
    • Related Report
      2009 Final Research Report
  • [Presentation] レトロウイルス遺伝子発現の不安定性の解析2007

    • Author(s)
      岡崎拓矢、山道信毅、水谷壮利、石坂彩、古川千尋、伊庭英夫
    • Organizer
      第30回日本分子生物学会年会第80回日本生化学会大会合同大会
    • Place of Presentation
      横浜
    • Related Report
      2009 Final Research Report
  • [Presentation] HIV-1のTat非依存的な発現におけるSWI/SNF複合体の機能解析2007

    • Author(s)
      水谷壮利、石坂彩、山道信毅、原口健、伊庭英夫
    • Organizer
      第55回日本ウイルス学会学術集会
    • Place of Presentation
      札幌
    • Related Report
      2009 Final Research Report
  • [Presentation] Brm, a catalytic subunit of the SWI/SNF complex is required for Tat-independent stable expression of HIV-12007

    • Author(s)
      Mizutani T, Ishizaka A, Yamamichi N, Okazaki T, Iba H.
    • Organizer
      第7回淡路島感染・免疫フォーラム
    • Place of Presentation
      兵庫
    • Related Report
      2009 Final Research Report
  • [Presentation] AP-1 triggered transcription of miR-21 through an evolutionally conserved mechanism Systems Biology2007

    • Author(s)
      Fujita S, Ito T, Mizutani T, Minoguchi S, Yamamichi N, Iba H.
    • Organizer
      Global Regulation of Gene Expression
    • Place of Presentation
      New York, USA
    • Related Report
      2009 Final Research Report
  • [Presentation] The Novel Role of Dnmt3L in Maintenance of DNA Methylation-Dependent Retroviral Gene Silencing2007

    • Author(s)
      箕口滋、伊庭英夫
    • Organizer
      理化学研究所発生再生科学総合研究センターCDB Symposium 2007
    • Place of Presentation
      神戸
    • Related Report
      2009 Final Research Report
  • [Presentation] 幹細胞におけるDNAメチル化の動態制御機構2007

    • Author(s)
      箕口滋
    • Organizer
      熊本大学発生医学研究センター21世紀COEリエゾンラボ研究会
    • Place of Presentation
      熊本
    • Related Report
      2009 Final Research Report
  • [Presentation] AP-1 triggered transcription of miR-21 through an evolutionally conserved mechanism2007

    • Author(s)
      Fujita, S., Ito, T., Mizutani, T., Minoguchi, S., Yamanichi, N., Iba, H.
    • Organizer
      Systems Biology: Global Regulation of Gene Expression 2007 April
    • Place of Presentation
      New York
    • Related Report
      2007 Annual Research Report
  • [Presentation] SWI/SNF chromatin remodeling complex and cancer2006

    • Author(s)
      Iba, H.
    • Organizer
      The 10th Korea-Japan Cancer Research Workshop
    • Place of Presentation
      Tokyo, Japan
    • Related Report
      2009 Final Research Report
  • [Presentation] SWI/SNF複合体の触媒サブユニットBrmはTERT遺伝子の転写と選択的スプライシングに関与する2006

    • Author(s)
      伊藤太二、渡部博貴、山道信毅、近藤俊輔、板垣千春、丹藤利夫、水谷壮利、原口健、櫻井浩平、藤田修二、泉友則、礒辺俊明、伊庭英夫
    • Organizer
      日本分子生物学会2006フォーラム(分子生物学の未来)
    • Place of Presentation
      名古屋
    • Related Report
      2009 Final Research Report
  • [Presentation] クロマチン構造変換因子SWI/SNF複合体のHIV-1発現維持機構の機能解析2006

    • Author(s)
      水谷壮利、石坂彩、山道信毅、岡崎拓矢、伊庭英夫
    • Organizer
      日本分子生物学会2006フォーラム
    • Place of Presentation
      名古屋
    • Related Report
      2009 Final Research Report
  • [Presentation] レトロウイルスプロモーターサイレンシングにおけるRNAi機構の役割の検証2006

    • Author(s)
      原口健、水谷壮利、伊庭英夫
    • Organizer
      第54回日本ウイルス学会学術集会
    • Place of Presentation
      名古屋
    • Related Report
      2009 Final Research Report
  • [Presentation] 多くのヒト癌で高レベルに発現するmir-21遺伝子のプロモーター解析とmir-21の標的遺伝子の同定2006

    • Author(s)
      伊庭英夫、藤田修二、伊藤太二、水谷壮利
    • Organizer
      第65回日本癌学会学術総会
    • Place of Presentation
      横浜
    • Related Report
      2009 Final Research Report
  • [Presentation] 胃癌におけるクロマチン構造変換因子SWI/SNF複合体の触媒サブユニットBrm欠失:癌化におけるBrm欠失の役割2006

    • Author(s)
      山道信毅、稲田健一、渡部博貴、山道(仁科)光恵、水谷壮利、矢作直久、一瀬雅夫、堤寛、小俣政男、伊庭英夫
    • Organizer
      第65回日本癌学会学術総会
    • Place of Presentation
      横浜
    • Related Report
      2009 Final Research Report
  • [Presentation] Structure of human miR-21 promoter that is driven by AP-1 Computational prediction of promoter regions of vertebrate miRNAs2006

    • Author(s)
      Fujita S, Ito T, Yamamichi-Nishina M, Iba H.
    • Organizer
      20th IUBMB International Congress of Biochemistry and Molecular Biology and 11th FAOBMB Congress
    • Place of Presentation
      Kyoto, Japan
    • Related Report
      2009 Final Research Report
  • [Presentation] レトロウイルスの発現維持に必須な宿主因子Brmの特異な生合成過程2005

    • Author(s)
      伊庭英夫、山道信毅、水谷壮利、原口健
    • Organizer
      第53回日本ウイルス学会学術集会
    • Place of Presentation
      横浜
    • Year and Date
      2005-11-22
    • Related Report
      2009 Final Research Report
  • [Presentation] ES細胞に感染したレトロウイルスに見られるDNAメチル化に依存した可逆的ジーンサイレンシング2005

    • Author(s)
      箕口滋、渡部博貴、水谷壮利、伊庭英夫
    • Organizer
      第28回日本分子生物学会年会
    • Place of Presentation
      福岡
    • Related Report
      2009 Final Research Report
  • [Presentation] p54nrbとSWI/SNF複合体の相互作用とその生化学的意義2005

    • Author(s)
      伊藤太二、渡部博貴、山道信毅、近藤俊輔、板垣千春、丹藤利夫、水谷壮利、原口健、藤田修二、泉友則、礒辺俊明、伊庭英夫
    • Organizer
      第28回日本分子生物学会年会
    • Place of Presentation
      福岡
    • Related Report
      2009 Final Research Report
  • [Presentation] Reversible switching of MSCV Proviral Expression Reflects Dynamics of DNA Methylation in Embryonic Stem Cells2005

    • Author(s)
      箕口滋、渡部博貴、水谷壮利、伊庭英夫
    • Organizer
      文部科学省特定領域研究「幹細胞の可塑性と未分化性維持機構」国際シンポジウム
    • Place of Presentation
      京都
    • Related Report
      2009 Final Research Report
  • [Presentation] ヒト癌細胞株で転写後抑制を受けているSWI/SNF複合体の触媒サブユニットBrmは、HDAC阻害剤により発現誘導され抗癌活性を示す2005

    • Author(s)
      山道信毅、水谷壮利、渡部博貴、伊藤太二、矢作直久、一瀬雅夫、小俣政男、伊庭英夫
    • Organizer
      第64回日本癌学会学術総会
    • Place of Presentation
      札幌
    • Related Report
      2009 Final Research Report
  • [Presentation] BrmとBRG1の発現を共に欠失するヒト非小細胞肺がん細胞株における神経特異的遺伝子群の発現誘導とその機構2005

    • Author(s)
      渡部博貴、水谷壮利、山道信毅、伊庭英夫
    • Organizer
      第64回日本癌学会学術総会
    • Place of Presentation
      札幌
    • Related Report
      2009 Final Research Report
  • [Presentation] Unique expression mechanisms of Brm protein, a key regulatory host factor for continuous retroviral expression2005

    • Author(s)
      Yamamichi N, Mizutani T, Yamamichi-Nishina M, Haraguchi T, Kameda T, Iba H.
    • Organizer
      第5回あわじしま感染症・免疫フォーラム
    • Place of Presentation
      兵庫
    • Related Report
      2009 Final Research Report
  • [Remarks]

    • URL

      http://www.ims.u-tokyo.ac.jp/div-host-parasite/Version1.html

    • Related Report
      2009 Final Research Report
  • [Remarks]

    • URL

      http://www.ims.u-tokyo.ac.jp/div-host-parasite/Version1.html

    • Related Report
      2009 Annual Research Report
  • [Remarks]

    • URL

      http://www.ims.u-tokyo.ac.jp/div-host-parasite/research.html

    • Related Report
      2007 Annual Research Report

URL: 

Published: 2005-04-01   Modified: 2018-03-28  

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