Tailor-made Antitumor Agents based on DNA Sequence

• Project/Area Number: 17016033
• Research Category: Grant-in-Aid for Scientific Research on Priority Areas
• Allocation Type: Single-year Grants
• Review Section: Biological Sciences
• Research Institution: Kyoto University
• Principal Investigator: SUGIYAMA Hiroshi Kyoto University, 大学院・理学研究科, 教授 (50183843)
• Co-Investigator(Kenkyū-buntansha): BANDO Toshikazu 京都大学, 大学院・理学研究科, 准教授 (20345284)
• Project Period (FY): 2005 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥46,900,000 (Direct Cost: ¥46,900,000); Fiscal Year 2009: ¥8,900,000 (Direct Cost: ¥8,900,000); Fiscal Year 2008: ¥8,900,000 (Direct Cost: ¥8,900,000); Fiscal Year 2007: ¥9,800,000 (Direct Cost: ¥9,800,000); Fiscal Year 2006: ¥9,800,000 (Direct Cost: ¥9,800,000); Fiscal Year 2005: ¥9,500,000 (Direct Cost: ¥9,500,000)
• Keywords: Py-Imポリアミド / テーラーメード抗がん剤 / 塩基配列認識 / 配列特異的アルキル化 / 遺伝子発現制御 / ピロール-イミダゾールポリアミド / 配列特異的DNAアルキル化

Research Abstract

Direct regulation of the expression level of a specific gene will provide a promising approach for cancer therapy. N-Methylpyrrole (Py) and N-methylimidazole (Im) polyamides are a new type of small molecules that precisely bind to the minor groove of the DNA duplex in a sequence-specific fashion. We designed and synthesized a series of sequence-specific alkylating Py-Im polyamide conjugates that selectively alkylate taget DNA sequences. We have demonstrated that sequence-specific alkylating agents possess gene-silencing activities and a promising potency against human cancer cell lines.

Research Products

• [Journal Article] Cooperative Alkylation of Double-strand Human Telomere Repeat Sequences by PI Polyamides with 11-Base-pair Recognition Based on a Heterotrimeric Design. (2009)
  • Author(s): Kashiwazaki, G.; Bando, T.; Shinohara, K.; Minoshima, M.; Nishijima, S.; Sugiyama, H.
  • Journal Title: Bioorg. Med. Chem. 17 Pages: 1393-1397
  • Peer Reviewed
• [Journal Article] A Novel Gene Silence, Pyrrole-Imidazole Polyamide Targeting Human Lectin-like Oxidized Low-density Lipoprotein Receptor-1 Gene Improves Endothelial Cell Function. (2009)
  • Author(s): Ueno, T.; Fukuda, N.; Tsunemi, A.; Yao, E.H.; Matsuda, H.; Tahira, K.; Matsumoto, T.; Matsumoto, Y.; Nagase, H.; Sugiyama, H.; Sawamura, T.
  • Journal Title: J. Hypertens. 27 Pages: 508-516
  • Peer Reviewed
• [Journal Article] Determination of Pyrrole-Imidazole Polyamide in Rat Plasma by Liquid Chromatography-Tandem Mass Spectrometry. (2009)
  • Author(s): Nagashima, T.; Aoyama, T.; Fukasawa, A.; Watabe, S.; Fukuda, N.; Ueno, T.; Sugiyama, H.; Nagase, H.; Matsumoto, Y.
  • Journal Title: J. Chromatogr. Analyt. Technol. Biomed. Life Sci. 877 Pages: 1070-1076
  • NAID: 10028015578
  • Peer Reviewed
• [Journal Article] Development of a Molecule-Recognized Promoter DNA Sequence for Inhibition of HER2 Expression. (2009)
  • Author(s): Suzuki, T.; Asami, Y.; Takahashi, T.; Wang, X.; Watanabe, T.; Bando, T.; Sugiyama, H.; Fukuda, N.; Nagase, H.
  • Journal Title: J. Antibiot. 62 Pages: 339-341
  • NAID: 10030635628
  • Peer Reviewed
• [Journal Article] Synthesis and Properties of PI Polyamide-SAHA Conjugate. (2009)
  • Author(s): Ohtsuki, A.; Kimura, M.T.; Minoshima, M.; Suzuki, T.; Ikeda, M.; Bando, T.; Nagase, H.; Shinohara, K.; Sugiyama, H.
  • Journal Title: Tetrahedron Lett. 50 Pages: 7288-7292
  • NAID: 120001741230
  • Peer Reviewed
• [Journal Article] Synthesis and Properties of Polyamide-SAHA Conjugate. (2009)
  • Author(s): Sugiyama, H., et al.
  • Journal Title: Tetrahedron Lett. 50 Pages: 7288-7292
  • Peer Reviewed
• [Journal Article] Cooperative alkylation of double-strand human telomere repeat sequences by PI polyamides with 11-base-pair recognition based on a heterotrimeric design (2009)
  • Author(s): Sugiyama, H., et al.
  • Journal Title: Bioorg. Med. Chem. 17 Pages: 1393-1397
  • Peer Reviewed
• [Journal Article] A Pyrrole-Imidazole Polyamide Targeting TGF-alInhibits Restenosis and Preserves Endothelialization in the Injured Artery (2009)
  • Author(s): Fukuda, N. , Sugiyama, H., et al.
  • Journal Title: Cardiovascular Research 81 Pages: 797-804
  • Peer Reviewed
• [Journal Article] Sequence-Specific Alkylation by Y-shaped and Tandem Hairpin Pyrrole-Imidazole Polyamides. (2008)
  • Author(s): Sasaki, S.; Bando, T.; Minoshima, M.; Shinohara, K.; Sugiyama, H.
  • Journal Title: Chem. Eur. J. 14 Pages: 864-870
  • Peer Reviewed
• [Journal Article] Requirement of β-Alanine Components in Sequence-Specific DNA Alkylation by Pyrrole-Imidazole Conjugates with Seven-Base Pair Recognition. (2008)
  • Author(s): Bando, T.; Minoshima, M.; Kashiwazaki, G.; Shinohara, K.; Sasaki, S.; Fujimoto, J.; Ohtsuki, A.; Murakami, M.; Nakazono, S.; Sugiyama, H.
  • Journal Title: Bioorg. Med. Chem. 16 Pages: 2286-2291
  • Peer Reviewed
• [Journal Article] Pyrrole-Imidazole Hairpin Polyamides with High Affinity at 5'-CGCG-3' DNA Sequence; Influence of Cytosine Methylation on Binding. (2008)
  • Author(s): Minoshima, M.: Bando, T.; Sasaki, S.; Fujimoto, J.; Sugiyama, H.
  • Journal Title: Nucleic. Acids. Res. 36 Pages: 2889-2894
  • Peer Reviewed
• [Journal Article] Detection of Triplet Repeat Sequences in the Double-Stranded DNA using Pyrene-Functionalized Pyrrole-Imidazole Polyamides with Rigid Linkers. (2008)
  • Author(s): Fujimoto, J.; Bando, T.; Uchida, S.; Iwasaki, M.; Shinohara, K.; Sugiyama, H.
  • Journal Title: Bioorg. Med. Chem. 16 Pages: 5899-5907
  • Peer Reviewed
• [Journal Article] A Novel Gene Silencer Pyrrole-Imidazole Polyamide Targeting LOX-1 Attenuates Restenosis of the Artery after Injury. (2008)
  • Author(s): Yao, E-H.; Fukuda, N.; Ueno, T.; Matsuda, M.; Matsumoto, M.; Nagase, H.; Matsumoto, Y.; Takasaka, A.; Serie, K.; Sugiyama, H.; Sawamura, T.
  • Journal Title: Hypertension 52 Pages: 86-92
  • Peer Reviewed
• [Journal Article] Development of Pyrrole-Imidazole Polyamide for Specific Regulation of Human Aurora Kinase A and B Gene Expression. (2008)
  • Author(s): Takahashi, T.; Asami, Y.; Kitamura, E.; Suzuki, T.; Wang, X.; Igarashi, J.; Morohashi, A.; Shinojima, Y.; Kanou, H.; Saito, K.; Takasu, T.; Nagase, H.; Harada, Y.; Kuroda, K.; Watanabe, T.; Kumamoto, S.; Aoyama, T.; Matsumoto, Y.; Bando, T.; Sugiyama, H.; Yoshida-Noro, C.; Fukuda, N.; Hayashi N.
  • Journal Title: Chem. Biol. 15 Pages: 829-841
  • Peer Reviewed
• [Journal Article] Perylene-Conjugated Pyrrole Polyamide as a Sequence-Specific Fluorescent Probe. (2008)
  • Author(s): Fujimoto, J.; Bando, T.; Minoshima, M.; Kashiwazaki, G.; Nishijima, S.; Shinohara, K.; Sugiyama, H.
  • Journal Title: Bioorg. Med. Chem. 16 Pages: 9741-9744
  • Peer Reviewed
• [Journal Article] Pyrrole-imidazole hairpin polyamides with high affinity at 5'-CGCG-3' DNA sequence ; Influence of cytosine methylation on binding (2008)
  • Author(s): Sugiyama H.; Bando, T.
  • Journal Title: Nucleic. Acids. Res 36 Pages: 2889-28941
• [Journal Article] Development of Pyrrole-Imidazole Polyaiide for Specific Regulation of Human Aurora kinase A and B Gene Expression (2008)
  • Author(s): Takahashi, T ; Nagase, H.; Sugiyama, H
  • Journal Title: Chemistry & Biology 15 Pages: 829-841
  • Peer Reviewed
• [Journal Article] Requirement of b-Alanine Components in Sequence-Specific DNA Alkylation by Pyrrole-Imidazole Conjugates with Seven-Base Pair Recognition (2008)
  • Author(s): Sugiyama H.; Bando, T.
  • Journal Title: Bioorgatic & Medicinal Chemistry 16 Pages: 2286-2291
  • Peer Reviewed
• [Journal Article] Seauence-Specific Alkylation by Y-shaped and Tandem Hairpin Pyrrole-Imidazole Polyamides (2008)
  • Author(s): Sugiyama H.; Bando, T.
  • Journal Title: Chem. Eur. J. 18 Pages: 864-870
  • Peer Reviewed
• [Journal Article] DNA Alkylation by Pyrrole-Imidazole seco-CBI Conjugates with an Indole Linker: Sequence-Specific DNA Alkylation with Ten-Base-Pair Recognition through Hetelodimer Formation. (2007)
  • Author(s): Minoshima, M.; Bando, T.; Sasaki, S.; Shinohara, K.; Shimizu, T.; Fujimoto, J.; Sugiyama, H.
  • Journal Title: J. Am. Chem. Soc. 129 Pages: 5384-5390
  • Peer Reviewed
• [Journal Article] Detection of CAG Repeat DNA Sequences by Pyrene-Functionalized Pyrrole-Imidazole Polyamides. (2007)
  • Author(s): Bando, T.;Fujimoto, J.; Minoshima, M.; Shinohara, K.; Sasaki, S.; Kashiwazaki, G.; Mizumura, M.; Sugiyama, H.
  • Journal Title: Bioorg. Med. Chem. 15 Pages: 6937-6942
  • Peer Reviewed
• [Journal Article] Photochemical Determination of Different DNA Structures (2007)
  • Author(s): Sugiyama, H; Xu, Y.
  • Journal Title: Nature Protocols 2 Pages: 78-87
  • Peer Reviewed
• [Journal Article] DNA Alkylation by Pyrrole-Imidazole seco-CBI Conjugates with an Indole Linker: Sequence-Specific DNA Alkylation (2007)
  • Author(s): Sugiyama H.; Bando, T.
  • Journal Title: J. Am. Chem. Soc. 129 Pages: 5384-5390
  • Peer Reviewed
• [Journal Article] Development of Gene Silencing Pyrrole-Imidazole Polyamide Targeting the TGF-β1 Promoter for Treatment of Progressive Renal Diseases. (2006)
  • Author(s): Matsuda, H.; Fukuda, N.; Ueno, T.; Tahira, Y.; Ayame, H.; Zhang, W.; Bando, T.; Sugiyama, H.; Saito, S.; Matsumoto, K.; Mugishima, H.; Serie, K.
  • Journal Title: J. Am. Soc. Nephr. 17 Pages: 422-432
  • NAID: 10024923283
  • Peer Reviewed
• [Journal Article] Antitumor Activity of Sequence-Specific Alkylating Agents: Pyrrole-Imidazole CBI Conjugates with Indole Linker. (2006)
  • Author(s): Shinohara, K.; Bando, T.; Sasaki, S.; Sakakibara, Y.; Minoshima, M.; Sugiyama, H.
  • Journal Title: Cancer Science. 97 Pages: 219-225
  • Peer Reviewed
• [Journal Article] Efficient DNA Alkylation by a Pyrrole-Imidazole CBI Conjugate with an Indole Linker: Sequence Specific Alkylation with Nine-Base-Pair Recognition. (2006)
  • Author(s): Bando, T.; Sasaki, S.; Minoshima, M.; Dohno, C.; Shinohara, K.; Narita, A.; Sugiyama, H.
  • Journal Title: Bioconjugate Chem. 17 Pages: 715-720
  • Peer Reviewed
• [Journal Article] Alkylation of Template Strand of Coding Region Causes Effective Gene Silencing. (2006)
  • Author(s): Shinohara, K.; Sasaki, S.; Minoshima, M.; Bando, T.; Sugiyama, H.
  • Journal Title: Nucleic Acids Res. 34 Pages: 1189-1195
  • Peer Reviewed
• [Journal Article] Discrimination of Hairpin Polyamides with anα-Substituted-γ-Aminobutyric Acid as a 5'-TG-3' Reader in DNA Minor Groove. (2006)
  • Author(s): Zhang, W.; Bando, T.; Sugiyama, H.
  • Journal Title: J. Am. Chem. Soc. 128 Pages: 8766-8776
  • Peer Reviewed
• [Journal Article] Synthesis and Biological Properties of Sequence-Specific DNA-Alkylating Pyrrole-Imidazole Polyamides. (2006)
  • Author(s): ando, T.; Sugiyama, H.
  • Journal Title: Acc. Chem. Res. 39 Pages: 935-944
  • Peer Reviewed
• [Journal Article] Sequence-Specific Alkylation of Double-Strand Human Telomere Repeat Sequence by Pyrrole-Imidazole Polyamides with Indole Linkers. (2006)
  • Author(s): Sasaki, S.; Bando, T.; Minoshima, M.; Shimizu, T.; Shinohara, K.; Takaoka, T.; Sugiyama, H.
  • Journal Title: J. Am. Chem. Soc. 128 Pages: 12162-12168
  • Peer Reviewed
• [Journal Article] Efficient DNA Alkylation by a Pyrrole-Imidazole CBI Conjugate with an Indole Linker : Sequence Specific Alkylation with Nine-Base-Pair Recognition. (2006)
  • Author(s): Sugiyama H., Bando, T.
  • Journal Title: Bioconjugate Chem. 17・3 Pages: 715-720
• [Journal Article] Discrimination of Hairpin Polyamides with an Alpha-Substituted-Gamma-Aminobutyric Acid as a 5'-TG-3' Reader in DNA Minor Groove. (2006)
  • Author(s): Sugiyama H., Bando, T.
  • Journal Title: J.Am.Chem.Soc. 128・27 Pages: 8766-8776
• [Journal Article] Sequence-Specific Alkylation of Double-Strand Human Telomere Repeat Sequence by Pyrrole-Imidazole Polyamides with Indole Linkers. (2006)
  • Author(s): Sugiyama H., Bando, T.
  • Journal Title: J.Am.Chem.Soc. 128・37 Pages: 12162-12168
• [Journal Article] Synthesis and Biological Properties of Sequence-Specific DNA-Alkylating Pyrrole-Imidazole Polyamides (2006)
  • Author(s): Sugiyama H., Bando, T.
  • Journal Title: Acc.Chem.Res. 39・12 Pages: 935-944
• [Journal Article] Base Pair Recognition of the Stereochemically alpha-Substituted gamma-turn of Pyrrole-Imidazole Hairpin Polyamides. (2006)
  • Author(s): Sugiyama H., Zhang W.
  • Journal Title: J.Am.Chem.Soc. 128 ・ 46 Pages: 14905-14912
• [Journal Article] The New Models of the Human Telomere d[AGGG(TTAGGG)3] in K+ Solution. (2006)
  • Author(s): Sugiyama H., Xu Y.
  • Journal Title: Bioorg.Med.Chem. 14 Pages: 5584-5591
• [Journal Article] Development of Gene Silencing Pyrrole-Imidazole Polyamide Targeting the TGF-β1 Promoter for Treatment of Progressive Renal Diseases. (2006)
  • Author(s): Sugiyama H., Bando, T., N.Fukuda
  • Journal Title: J.Am.Soc.Nephr. 17・Feb Pages: 422-432
  • NAID: 10024923283
• [Journal Article] Antitumor Activity of Sequence-specific Alkylating Agents : Pyrrole-imidazole CBI conjugates with Indole linker (2006)
  • Author(s): Sugiyama H., K.Shinohara
  • Journal Title: Cancer Science 97・3 Pages: 219-225
• [Journal Article] Alkylation of templete strand of coding region causes effective gene silencing (2006)
  • Author(s): Sugiyama H., K.Shinohara
  • Journal Title: Nucleic Acids Research 34・4 Pages: 1189-1195
• [Journal Article] Synthetic Pyrrole-Imidazole Polyamide Inhibits Expression of the Human Transforming Growth Factor-β1 Gene. (2005)
  • Author(s): Lai, Y. -M.; Fukuda, N.; Ueno, T.; Kishioka, H.; Matsuda, H.; Saito, S.; Matsumoto, K.; Ayame, H.; Bando, T.; Sugiyama, H.; Mugishima, H.; Kim, S. -J.
  • Journal Title: J. Pharmacol. Exp. Ther. 315 Pages: 571-575
  • Peer Reviewed
• [Journal Article] Specific Adenine Alkylation by Pyrrole-Imidazole CBI Conjugates. (2005)
  • Author(s): Bando, T.; Narita, A.; Sasaki, S.; Sugiyama, H.
  • Journal Title: J. Am. Chem. Soc. 127 Pages: 13890-13895
  • Peer Reviewed
• [Journal Article] Synthetic Pyrrole-Imidazole Polyamide Inhibits Expression of the Human Transforming Growth Factor-β1 Gene. (2005)
  • Author(s): Sugiyama H., Bando, T., N.Fukuda
  • Journal Title: J.Pharmacol.Exp.Ther. 315・2 Pages: 571-575
• [Journal Article] Specific Adenine Alkylation by Pyrrole-Imidazole CBI Conjugates (2005)
  • Author(s): Sugiyama H., Bando, T.
  • Journal Title: J.Am.Chem.Soc. 127・40 Pages: 13890-13895
• [Journal Article] The Chemical Biology That Controls DNA Function and Structure (2005)
  • Author(s): Sugiyama H., Bando, T.
  • Journal Title: 有機合成化学協会誌 63・10 Pages: 1016-1027
  • NAID: 130000928679
• [Presentation] 人工遺伝子スイッチによる細胞制御 (2010)
  • Author(s): 杉山弘
  • Organizer: 日本化学会弟90春季年会
  • Place of Presentation: 大阪
• [Presentation] 人工遺伝子スイッチによる細胞制御 (2010)
  • Author(s): 杉山弘
  • Organizer: 日本化学会弟90春季年会
  • Place of Presentation: 大阪近畿大学
• [Presentation] 遺伝子発現を制御するPIポリアミド (2009)
  • Author(s): 杉山弘
  • Organizer: 「生体調節のケミカルバイオロジー」シンポジウム
  • Place of Presentation: 前橋市
  • Year and Date: 2009-03-27
• [Presentation] Synthesis and Biological Properties of Sequence-Specific DNA-alkylating Pyrrole-Imidazole Polyamides (2009)
  • Author(s): H. Sugiyama
  • Organizer: The Next Generation Japanese Technology Showcase
  • Place of Presentation: New York, USA
• [Presentation] 遺伝子発現を制御するPIポリアミド, 「生体調節のケミカルバイオロジー」シンポジウム (2009)
  • Author(s): 杉山弘
  • Place of Presentation: 前橋市
• [Presentation] 塩基配列特異的アルキル化能を有する機能性PIポリアミド設計 (2009)
  • Author(s): 板東俊和, 杉山弘
  • Organizer: 第28回メディシナルケミストリーシンポジウム
  • Place of Presentation: 東京
• [Presentation] Design and Construction of DNA Nanostructures for Integration of Molecules (2009)
  • Author(s): H. Sugiyama
  • Organizer: International Symposium Watching Biomolecules in Action: Single Molecule Biology Symposium
  • Place of Presentation: Osaka
• [Presentation] ゲノム情報をあやつる有機化学 (2008)
  • Author(s): 杉山 弘
  • Organizer: SORSTジョイントシンポジウム(8)
  • Place of Presentation: コクヨホール(品川)
  • Year and Date: 2008-01-30
• [Presentation] 特定DNA塩基配列を標的とする機能性ポリアミドの開発 (2008)
  • Author(s): 板東俊和, 杉山弘
  • Organizer: 薬学会第128年会
  • Place of Presentation: 横浜
• [Presentation] Chemical biology that controls DNA structure and function (2008)
  • Author(s): H. Sugiyama
  • Organizer: International Symposium on Molecular Recognition of DNA: Biological Applications
  • Place of Presentation: Tokyo
• [Presentation] Pyrrole-Imidazole Polyamide as a Genetic Switch (2008)
  • Author(s): H. Sugiyama
  • Organizer: Chemical Biology 2008
  • Place of Presentation: Heidelberg, Germany
• [Presentation] Chemical Biology that Controls DNA Structure and Function (2008)
  • Author(s): H. Sugiyama
  • Organizer: 10th KAIST-KYOTO Chemistry Symposium
  • Place of Presentation: Daejeon, Korea
• [Presentation] DNAの塩基配列と構造を読む分子, 平成18年度科学交流フォーラム-医学を支える生命科学の最前線- (2007)
  • Author(s): 杉山弘
  • Place of Presentation: 静岡
• [Presentation] DNAを分子標的とした薬剤の設計 (2007)
  • Author(s): 杉山弘
  • Organizer: 日本薬学会北陸支部平成19年度特別講演会
  • Place of Presentation: 金沢
• [Presentation] Chemistry that Controls DNA Structure and Function (2007)
  • Author(s): H. Sugiyama
  • Organizer: East China University of Science and Technology
  • Place of Presentation: Shanghai, China
• [Presentation] Chemical biology that controls DNA structure and function (2007)
  • Author(s): H. Sugiyama
  • Organizer: The 5th Internationa. Symposium on Nucleic Acids Chemistry
  • Place of Presentation: Tokyo
• [Presentation] Selective Gene Silencing by Alkylating Pyrrole-Imidazole Polyamide (2006)
  • Author(s): H. Sugiyama
  • Organizer: KEYSTONE SYMPOSIA
  • Place of Presentation: Taos, New Mexico, USA
• [Presentation] dna配列を標的とするアルキル化ピロール-イミダゾールポリアミドの開発 (2006)
  • Author(s): 板東俊和, 杉山弘
  • Organizer: 第10回がん分子標的治療研究会総会
  • Place of Presentation: 東京
• [Presentation] dnaを中心としたケミカルバイオロジー (2005)
  • Author(s): 杉山弘
  • Organizer: 第3回ケモゲノミクス研究会
  • Place of Presentation: 京都
• [Presentation] "A Sequence-Specific DNA Alkylating Polyamide, Toward a Tailor-Made Antitumor Agent" Albany 2005 (2005)
  • Author(s): H. Sugiyama
  • Organizer: Conversation
  • Place of Presentation: Albany, USA
• [Book] ウィーバー分子生物学第4版 (2008)
  • Author(s): R.F. Weaver著, 杉山弘・井上丹・森井孝 監訳
  • Total Pages: 1022
  • Publisher: 化学同人
• [Book] ウィーバー分子生物学第4版 (2008)
  • Author(s): R. F. Weaver 著、杉山弘 監訳
  • Total Pages: 1022
  • Publisher: 化学同人
• [Book] ゲノム化学-医学、分子生物学への応用と展開 (2007)
  • Author(s): 齋藤烈, 杉山弘, 中谷和彦
  • Total Pages: 214
  • Publisher: 化学同人
• [Book] ゲノム化学 医学、分子生物学への応用と展開 (2007)
  • Author(s): 齋藤 烈, 杉山 弘, 中谷和彦
  • Total Pages: 214
  • Publisher: 化学同人
• [Remarks] 研究業績・論文リスト参照
  • URL: http://kuchem.kyoto-u.ac.jp/chembio/
• [Remarks]
  • URL: http://kuchem.kyoto-u.ac.jp/chembio/publication/all.html#2009
• [Remarks] 論文リスト
  • URL: http://kuchem.kvoto-u.ac.ip/chembio/Chemicalaage/publication2008.htm
• [Remarks] 論文リスト
  • URL: http://kuchem.kyoto-u.ac.jp/chembio/publication/all.html#2007
• [Patent(Industrial Property Rights)] 標的遺伝子特異的ヒストン修飾制御剤 (2008)
  • Inventor(s): 永瀬浩喜, 杉山弘
  • Industrial Property Rights Holder: 学校法人日本大学
  • Filing Date: 2008-07-01
  • Acquisition Date: 2008-07-01
• [Patent(Industrial Property Rights)] インターストランドクロスリンク剤の合成方法 (2008)
  • Inventor(s): 杉山弘
  • Industrial Property Rights Holder: 科学技術振興機構
  • Filing Date: 2008-01-11
  • Acquisition Date: 2008-01-11
• [Patent(Industrial Property Rights)] 標的遺伝子特異的ピストン修飾制御剤 (2008)
  • Inventor(s): 永瀬浩喜, 杉山弘
  • Industrial Property Rights Holder: 学校法人日本大学
  • Industrial Property Number: 2008-172795
  • Filing Date: 2008-07-01
• [Patent(Industrial Property Rights)] インターストランドクロスリンク剤の合成方法 (2008)
  • Inventor(s): 杉山 弘
  • Industrial Property Rights Holder: 科学技術振興機構
  • Filing Date: 2008-01-11