Systems genomics in Drosophila

• Project/Area Number: 17017032
• Research Category: Grant-in-Aid for Scientific Research on Priority Areas
• Allocation Type: Single-year Grants
• Review Section: Biological Sciences
• Research Institution: Tokyo Metropolitan University
• Principal Investigator: AIGAKI Toshiro Tokyo Metropolitan University, 大学院・理工学研究科, 教授 (80150879)
• Co-Investigator(Kenkyū-buntansha): HAYASHI Shigeo 理化学研究所, CDB, グループディレクター (60183092) ; UEDA Ryu 国立遺伝学研究所, 系統生物センター, 教授 (40353429)
• Project Period (FY): 2005 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥178,000,000 (Direct Cost: ¥178,000,000); Fiscal Year 2009: ¥37,400,000 (Direct Cost: ¥37,400,000); Fiscal Year 2008: ¥35,300,000 (Direct Cost: ¥35,300,000); Fiscal Year 2007: ¥34,600,000 (Direct Cost: ¥34,600,000); Fiscal Year 2006: ¥34,700,000 (Direct Cost: ¥34,700,000); Fiscal Year 2005: ¥36,000,000 (Direct Cost: ¥36,000,000)
• Keywords: ゲノム / 遺伝子 / 表現型 / データベース / ショウジョウバエ / 遺伝子強制発現 / RNAi / 形態 / ストレス感受性 / 画像データ / 組織サイズ / ライブイメージング / 遺伝子強制 / 強制発現システム / 翅 / インスリンシグナリング / カスパーゼシグナリング / 強制発現ベクター / 強制発現変異体 / Gene Searchシステム / GS系統 / GSベクター / GAL4-UASシステム / 表現型情報

Research Abstract

Drosophila melanogaster is a model organism that have been extensively studied for their genes' functions through both forward and reverse genetic approaches. However, it has been estimated that only one-third of all genes in the genome could be mutated to easily detectable phenotypes, such as lethality, sterility or morphological abnormality. In order to reveal novel gene functions that may not be revealed with conventional approaches, we develop methods involving computational image analyses of adult wings and time-lapse confocal microscopic images of embryogenesis. To screen and identify new genes and/or new functions of known genes, we established and utilized a library of Gene Search (GS) lines and inducible RNAi lines as a resource to screen gain-of-function and reduction-of-function mutations. We identified and characterized various genes that include those involved in morphogenesis, immune response, stress resistance and longevity.

Research Products

• [Journal Article] a Drosophila melanogaster 5'-end mRNA transcription database (2009)
  • Author(s): Ahsan, B., Saito, TL., Hashimoto, S., Muramatsu, K., Tsuda, M., Sasaki, A., Matsushima, K., Aigaki, T., Morishita, S.
  • Journal Title: Nucleic Acids Res. 37
• [Journal Article] Mitochondrial Protein Preli-like Is Required for Development of Dendritic Arbors and Prevents Their Regression in the Drosophila Sensory Nervous System. (2009)
  • Author(s): Tsubouchi, A., Tsuyama, T., Fujioka, M., Kohda, H., Okamoto-Furuta, K., Aigaki, T., Uemura, T.
  • Journal Title: Development 136 Pages: 3757-3766
• [Journal Article] The Drosophila TRPA channel, Painless, regulates sexual receptivity in virgin females. (2009)
  • Author(s): Sakai, T., Kasuya, J., Kitamoto, T., Aigaki, T.
  • Journal Title: Genes Brain Behav. 8 Pages: 546-557
• [Journal Article] Aberrant E2F activation by polyglutamine expansion of androgen receptor in SBMA neurotoxicity. (2009)
  • Author(s): Suzuki, E., Zhao Y., Ito, S., Sawatsubashi, S., Murata, T., Furutani, T., Shirode, Y., Yamagata, K., Tanabe, M., Kimura, S., Ueda, T., Fujiyama, S., Lim, J., Matsukawa, H., Kouzmenko, AP., Aigaki, T., Tabata, T., Takeyama, KI., Kato, S.
  • Journal Title: Proc Natl Acad Sci USA. 106 Pages: 3818-3822
• [Journal Article] Essential requirement for RING finger E3 ubiquitin ligase Hakai in early embryonic development of Drosophila. (2009)
  • Author(s): Kaido, M., Wada, H., Shindo, M., Hayashi, S.
  • Journal Title: Genes to Cells 14 Pages: 1067-1077
• [Journal Article] A Blood-Borne PDGF/VEGF-like Ligand Initiates Wound-Induced Epidermal Cell Migration in Drosophila Larvae (2009)
  • Author(s): Wu, Y., Brock, A.R., Wang, Y., Fujitani, K., Ueda, R., Galko, M.J.
  • Journal Title: Curr. Biol. 19 Pages: 1-5
• [Journal Article] RNAi-Mediated Knockdown Showing Impaired Cell Survival in Drosophila Wing Imaginal Disc (2009)
  • Author(s): Umemori, M., Habara, O., Iwata, T., Maeda, K., Nishinoue, K., Okabe, A., Takemura, M., Takahashi, K., Saigo, K., Ueda, R., Adachi-Yamada, T.
  • Journal Title: Gene Regulation and Systems Biology 3 Pages: 11-20
• [Journal Article] Overexpression of grappa encoding a histone methyltransferase enhances stress resistance in Drosophila (2009)
  • Author(s): Olivier, L., Togawa, T., Tsuda, M., Matsuo, T., Loic, E., Aigaki, T.
  • Journal Title: Hereditas 146 Pages: 19-28
• [Journal Article] Autophagic control of listeria through intracellular innate immune recognition in Drosophila. (2009)
  • Author(s): Yano, T., Mita, S., Ohmori, H., Oshima, Y., Fujimoto, Y., Ueda, R., Takada, H., Goldman, WE., Fukase, K., Silverman, N., Yoshimori, T., Kurata, S.
  • Journal Title: at Immunol 9(8) Pages: 908-916
• [Journal Article] MachiBase : a Drosophila melanoogasxter 5'-end mRNA transcription database. (2009)
  • Author(s): Ahsan, B., et al
  • Journal Title: Nucleic Acids Res. 37
  • Peer Reviewed
• [Journal Article] Mitochondrial Protein Preli-like Is Required for Development of Dendritic Arbors and Prevents Their Regression in the Drosophila Sensory Nervous System. (2009)
  • Author(s): Tsubouchi, A., et al
  • Journal Title: Development 136 Pages: 3757-3766
  • Peer Reviewed
• [Journal Article] The Drosophila TRPA channel, Painless, regulates sexual receptivity in virgin females. (2009)
  • Author(s): Sakai, T., Kasuya, J., Kitamoto, T., Aigaki, T
  • Journal Title: Genes Brain Behay. 8 Pages: 546-557
  • Peer Reviewed
• [Journal Article] Essential requirement for RING finger E3 ubiquitin ligase Hakai in early embryonic develonment of Drosophila. (2009)
  • Author(s): Kaido, M., Wada, H., Shindo, M., Hayashi, S.
  • Journal Title: Genes to Cells. 14 Pages: 1067-1077
  • Peer Reviewed
• [Journal Article] Blood-Borne PDGF/VEGF-like Ligand Initiates Wound-Induced Epidermal Cell Migration in Drosophila Larvae. (2009)
  • Author(s): Wu, Y., Brock, A., et al
  • Journal Title: Curr.Biol. 19 Pages: 1-5
  • Peer Reviewed
• [Journal Article] RNAi-Mediated Knockdown Showing Impaired Cell Survival in Drosophila Wing Imaginal Disc. (2009)
  • Author(s): Umemori, M., et al
  • Journal Title: Gene Regulation and Systems Biology 3 Pages: 11-20
  • Peer Reviewed
• [Journal Article] Overexpression of grappa encoding a histone methyltransferase enhances stress resistance in Drosophila (2009)
  • Author(s): List O, Togawa T, Tsuda M, Matsuo T, Elard L, Aigaki T
  • Journal Title: Hereditas 146(1) Pages: 19-28
  • Peer Reviewed
• [Journal Article] Aberrant E2F activation by polyglutamine expansion of androgen receptor in SBMA neurotoxicity (2009)
  • Author(s): Suzuki, E., et.al
  • Journal Title: Proc Natl Acad Sci USA 106 Pages: 3818-3822
  • Peer Reviewed
• [Journal Article] MachiBase: a Drosophila melanogaster 5'-end mRNA transcription database (2009)
  • Author(s): Ahsan, B., et.al.
  • Journal Title: Nucleic Acids Res 37
  • Peer Reviewed
• [Journal Article] Protein kinase A inhibits a consolidated form of memory in Drosophila (2008)
  • Author(s): Horiuchi, J., Yamazaki, D., Naganos, S., Aigaki, T., Saitoe, M.
  • Journal Title: Proc Natl Acad Sci U S A 105 Pages: 20976-20981
• [Journal Article] Behavioral analyses of mutants for two odorant-binding protein genes, Obp57d and Obp57e, in Drosophila melanogaster. (2008)
  • Author(s): Harada, E., Haba, D., Aigaki, T., Matsuo, T.
  • Journal Title: Genes Genet Syst 83 Pages: 257-264
  • NAID: 10024396266
• [Journal Article] A wave of EGFR signaling determines cell alignment and intercalation in the Drosophila tracheal placode (2008)
  • Author(s): Nishimura, M., Inoue, Y., Hayashi, S.
  • Journal Title: Development 134(23) Pages: 4273-4282
• [Journal Article] Dual function of Src in the maintenance of adherens junction during epithelial morphogenesis (2008)
  • Author(s): Shindo, M., Wada, H., Kaido, M., Tateno, M., Aigaki, T., Tsuda, L., Hayashi, S.
  • Journal Title: Development 135(7) Pages: 1355-64
• [Journal Article] Conversion of plasma membrane topology during epithelial tube connection requires Arf-like 3 small GTPase in Drosophila (2008)
  • Author(s): Kakihara, K., Shinmyozu, K., Kato, K., Wada, H., Hayashi, S.
  • Journal Title: Mechanisms of Development 125(3-4) Pages: 325-336
• [Journal Article] Practical guide of live imagining for developmental biologists, Development (2008)
  • Author(s): Kato, K., Hayashi, S.
  • Journal Title: Growth and Differentiation
• [Journal Article] Identification of the Drosophila core 1β1, 3-galactosyltransferase gene that synthesizes T antigen in the embryonic central nervous system and hemocytes. (2008)
  • Author(s): Yoshida, H., Fuwa, T., Arima, M., Hamamoto, H., Sasaki, N., Ichimiya, T., Osawa, K-I., Ueda, R., Nishihara, S.
  • Journal Title: Glycobiology 18(12) Pages: 1094-1104
• [Journal Article] Practical guide of live imaging for developmental biologists (2008)
  • Author(s): Kato K, Hayashi S
  • Journal Title: Dev Growth Differ 50(6) Pages: 381-390
  • NAID: 10021096887
  • Peer Reviewed
• [Journal Article] Transposition of the vertebrate To12 transposable element in Drosophila melanogaster (2008)
  • Author(s): Urasaki A, Mito T, Noji S, Ueda R, Kawakami K
  • Journal Title: Gene 425(1-2) Pages: 64-68
  • Peer Reviewed
• [Journal Article] Behavioral analyses of mutants for two odorant-binding protein genes, Obp 57d and Obp57e, in Drosophila melanogaster (2008)
  • Author(s): Harada, E. Haba, D., Aigaki, T., Matsuo, T
  • Journal Title: Genes Genet. Syst 83 Pages: 257-264
  • NAID: 10024396266
  • Peer Reviewed
• [Journal Article] Protein kinase A inhibits a consolidated form of memory in Drosophila (2008)
  • Author(s): Horiuchi, J., Yamazaki, D., Naganos, S.. Aieaki. T. and Saitoe. M
  • Journal Title: Proc Natl Acad Sci USA 105 Pages: 20976-20981
  • Peer Reviewed
• [Journal Article] Dual function of Src in the maintenance of adherens junctions during tra cheal epithelial morphogenesis (2008)
  • Author(s): Shindo, M., et.al.
  • Journal Title: Development 135 Pages: 1355-1364
  • Peer Reviewed
• [Journal Article] TCA回路を中心とした代謝調節と寿命制御 (2008)
  • Author(s): 木下善仁, 津田学, 相垣敏郎
  • Journal Title: 基礎老化研究 32 Pages: 21-23
  • NAID: 10024377815
  • Peer Reviewed
• [Journal Article] Conversion of plasma membrane topology during epithelial tube connection requires Arf-like 3 small GTPase in Drosophila (2008)
  • Author(s): Kakihara K, Shinmyozu K, Kato K, Wada H, Hayashi S
  • Journal Title: Mech Dev 125(3-4) Pages: 4273-4282
  • Peer Reviewed
• [Journal Article] Autophagic control of listeria through intracellular innate immune recognition in drosophila (2008)
  • Author(s): Yano T., et.al
  • Journal Title: Nat Immunol 9(8) Pages: 908-916
  • Peer Reviewed
• [Journal Article] Natural variation of ebony gene controlling thoracic pigmentation in Drosophila melanogaster. (2007)
  • Author(s): Takahashi, A., Takahashi, K., Ueda, R., Takano-Shimizu, T.
  • Journal Title: Genetics 177(2) Pages: 1233-1237
• [Journal Article] Light Activates Output from Evening Neurons and Inhibits Output from Morning Neurons in the Drosophila Circadian Clock. (2007)
  • Author(s): Picot, M., Cusumano, P., Klarsfeld, A., Ueda, R., Rouyer, F.
  • Journal Title: PLoS Biol. 5(11)
• [Journal Article] Odorant-binding proteins OBP57d and OBP57e affect taste perception and host-plant preference in Drosophila sechellia. (2007)
  • Author(s): Matsuo, T., Sugaya, S., Yasukawa, J., Aigaki, T., Fuyama, Y.
  • Journal Title: PLoS Biol. 5
• [Journal Article] Thioredoxin suppresses Parkin-associated Endothelin Receptor-like Recepto-induced neurrotoxicity and extends longevity in Drosophila. (2007)
  • Author(s): Umeda-Kameyama, Y., Tsuda, M., Ohkura, C., Matsuo, T., Namba, Y., Ohuchi, Y., Aigaki, T.
  • Journal Title: J. Biol. Chem. 282 Pages: 11180-11187
• [Journal Article] Antagonistic roles of Wnt5 and the Drl receptor in patterning the Drosophila antennal lobe. (2007)
  • Author(s): Yao, Y., Wu, Y., Yin, C., Ozawa, R., Aigaki, T., Wouda, R.R., Noordermeer, J.N., Fradkin, L.G., Hing, H.
  • Journal Title: Nat Neurosci. 10 Pages: 1423-1432
• [Journal Article] A mev-1-like dominant-negative SdhC increases oxidative stress and reduces lifespan in Drosophila. (2007)
  • Author(s): Tsuda M, Sugiura T, Ishii T, Ishii N, Aigaki T.
  • Journal Title: Biochem Biophys Res Commun. 363 Pages: 342-346
• [Journal Article] Tissue-specific laminin expression facilitates integrin-dependent association of the embryonic wing disc with the trachea in Drosophila. (2007)
  • Author(s): Inoue, Y., Hayashi, S.
  • Journal Title: Developmental Biology 304 Pages: 90-101
• [Journal Article] Small peptide regulators of actin-based cell morphogenesis encoded by a polycistronic mRNA (2007)
  • Author(s): Kondo, T., Hashimoto, T., Kato, K., Inagaki, S., Hayashi, S., Kageyama, Y.
  • Journal Title: Nature Cell Biology 9(6) Pages: 660-665
• [Journal Article] Differential control of cell affinity required for progression and refinement of cell boundary during Drosophila leg segmentation (2007)
  • Author(s): Sakurai, T, K., Kojima, T., Aigaki, T., Hayashi, S.
  • Journal Title: Developmental Biology
• [Journal Article] A female-biased expressed elongase involved in long-chain hydrocarbon biosynthesis and courtship behavior in Drosophila melanogaster. (2007)
  • Author(s): Chertemps, T., Duportets, L., Labeur, C., Ueda, R., Takahashi, K., Saigo, K., Wicker-Thomas, C.
  • Journal Title: Proc Natl Acad Sci USA 104(11) Pages: 4273-4278
• [Journal Article] ATF-2 regulates fat metabolism in Drosophila. (2007)
  • Author(s): Okamura, T., Shimizu, H., Nagao, T., Ueda, R., Ishii, S.
  • Journal Title: Mol Biol Cell 18(4) Pages: 1519-1529
• [Journal Article] A mev-l-like dominant-negative SdhC increases oxidative stress and reduces lifespan in Drosophila (2007)
  • Author(s): Tsuda M, Sugiura T, Ishii T, Ishii N, Aigaki T.
  • Journal Title: Biochem Biophys Res Commun 363 Pages: 342-346
  • Peer Reviewed
• [Journal Article] Tissue-specific laminin expression facilitates integrin-dependent association of the embryonic wing disc with the trachea in Drosophila (2007)
  • Author(s): Inoue, Y., Hayashi, S.
  • Journal Title: Developmental Biology 304 Pages: 90-101
  • Peer Reviewed
• [Journal Article] ATF-2 regulates fat metabolism in Drosophila (2007)
  • Author(s): Okamura, T, Shimizu, H, Nagao, T, Ueda R, and Ishii, S.
  • Journal Title: Mol Biol Cell 18(4) Pages: 1519-1529
  • Peer Reviewed
• [Journal Article] Thioredoxin Suppresses Parkin-associated Endothelin Receptor-like Receptor-induced Neurotoxicity and Extends Longevity in Drosophila (2007)
  • Author(s): Umeda-Kameyama Y, Tsuda M, Ohkura C, Matsuo T, Namba Y, Ohuchi Y, Aigaki T
  • Journal Title: Biol Chem 282 Pages: 11180-11187
• [Journal Article] The calcineurin regulator Sra plays an essential role in female meiosis in Drosophila (2006)
  • Author(s): Takeo, S., Tsuda, M., Akahori, S., Matsuo, T., Aigaki, T.
  • Journal Title: Current Biology 16 Pages: 1435-1440
• [Journal Article] POSH, a scaffold protein for JNK signaling, binds to ALG-2 and ALIX in Drosophila (2006)
  • Author(s): Tsuda, M., Seong K. -H., Aigaki, T.
  • Journal Title: FEBS letters 580 Pages: 3296-300
• [Journal Article] An unconventional myosin in Drosophila reverses the default handedness in visceral organs (2006)
  • Author(s): Hozumi, S., Maeda, R., Taniguchi, K., Kanai, M., Shirakabe, S., Sasamura, T., Speder, P., Noselli, S., Aigaki, T., Murakami, R., Matsuno, K.
  • Journal Title: Nature 440 Pages: 798-802
• [Journal Article] Regulation of Layer-Specific Targeting by Reciprocal Expression of a Cell Adhesion Molecule, Capricious. (2006)
  • Author(s): Shinza-Kameda, M., Takasu, E., Sakurai, K., Hayashi, S., Nose, A.
  • Journal Title: Neuron 49 Pages: 205-213
• [Journal Article] Drosophila IKK-Related Kinase Regulates Nonaapoptotic Function of Caspases via Degradation of IAPs (2006)
  • Author(s): Kuranaga, E., Kanuta, H., Tonoki, A., Takemoto, K., Tomioka, T., Kobayashi, M., Hayashi., S., Miura, M.
  • Journal Title: Cell 126 Pages: 583-596
• [Journal Article] IKKεRegulates F Actin Assembly and Interacts with Drosophila IAP1 in Cellular Morphogenesis (2006)
  • Author(s): Oshima, K., Takeda, M., Kuranaga, E., Ueda, R., Aigaki, T., Miura, M., Hayashi, S.
  • Journal Title: Current Biology 16 Pages: 1531-1537
• [Journal Article] An NRSF/REST-like repressor downstream of Ebi/SMRTER/Su(H) regulates eye development in Drosophila (2006)
  • Author(s): Tsuda, L., Kaido, M., Young-Mi Lim., Kato, K., Aigaki T., Hayashi, S.
  • Journal Title: The EMBO Journal 25 Pages: 3191-3202
• [Journal Article] Differential microarray analysis of Drosophila mushroom body transcripts using chemical ablation. (2006)
  • Author(s): Kobayashi M, Michaut L, Ino A, Honjo K, Nakajima T, Maruyama Y, Mochizuki H, Ando M, Ghangrekar I, Takahashi K, Saigo, K., Ueda, R., Gehring, W.J., Furukubo-Tokunaga, K.
  • Journal Title: Proc Natl Acad Sci USA 103(39) Pages: 14417-22
• [Journal Article] Control of axonal sprouting and dendrite branching by the Nrg-Ank complex at the neuron-glia interface (2006)
  • Author(s): Yamamoto, M., Ueda, R., Takahashi, K., Saigo, K., Uemura, T.
  • Journal Title: Curr Bio 16(16) Pages: 1678-83
• [Journal Article] Essential role of the apoptotic cell engulfment genes draper and ced-6 in programmed axon pruning during Drosophila metamorphosis. (2006)
  • Author(s): Awasaki, T., Tatsumi, R., Takahashi, K., Arai, K., Nakanishi, Y., Ueda, R., Ito, K.
  • Journal Title: Neuron 49 Pages: 855-67
• [Journal Article] Drosophila immunity: a large-scale in vivo RNAi screen identifies five serine proteases required for Toll activation. (2006)
  • Author(s): Kambris, Z., Brun, S., Jang, IH., Nam, HJ., Romeo, Y., Takahashi, K., Lee, WJ., Ueda, R., Lemaitre, B.
  • Journal Title: Curr Biol. 16 Pages: 808-813
• [Journal Article] Alternative trans-splicing: a novel mode of pre-mRNA processing. (2006)
  • Author(s): Horiuchi, T., Aigaki, T.
  • Journal Title: Biol. Cell 98 Pages: 135-140
• [Journal Article] Regulation of Layer-Specific Targeting by Reciprocal Expression of a Cell Adhesion Molecule (2006)
  • Author(s): Shinza-Kameda, M., Takasu, E., Sakurai, K., Hayashi, S., Nose, A.
  • Journal Title: Capricious. Neuron 49 Pages: 205-213
• [Journal Article] IKK epsilon regulates F actin assembly and interacts with Drosophila IAP1 in cellular morphogenesis. (2006)
  • Author(s): Oshima K, Takeda M, Kuranaga E, Ueda R, Aigaki T, Miura M, Hayashi S
  • Journal Title: Curr Biol 16 Pages: 1531-1537
• [Journal Article] The calcineurin regulator sra plays an essential role in female meiosis in Drosophila. (2006)
  • Author(s): Takeo S, Tsuda M, Akahori S, Matsuo T, Aigaki T
  • Journal Title: Curr Biol 16 Pages: 1435-1440
• [Journal Article] An NRSF/REST like repressor downstream of Ebi/SMRTER/Su(H) regulates eye development in Drosophila. (2006)
  • Author(s): Tsuda L, Kaido M, Lim YM, Kato K, Aigaki T, Hayashi S
  • Journal Title: EMBOJ 25 Pages: 3191-3202
• [Journal Article] POSH, a scaffold protein for JNK signaling, binds to ALG-2 and ALIX in Drosophila. (2006)
  • Author(s): Tsuda M, Seong KH, Aigaki T
  • Journal Title: FEBS Lett 580 Pages: 3296-3300
• [Journal Article] An unconventional myosin in Drosophila reverses the default handedness in visceral organs. (2006)
  • Author(s): Hozumi S, Maeda R, Taniguchi K, Knanai M, Shirakabe S, Sasamura T, Speder P, Noselli S, Aigaki T, Murakami R, Matsuno K
  • Journal Title: Nature 440 Pages: 798-802
• [Journal Article] Alternative trans-splicing : a novel mode of pre-mRNA processing. (2006)
  • Author(s): Horiuchi, T., Aigaki, T.
  • Journal Title: Biol. Cell 98 Pages: 135-140
• [Journal Article] A Genetic screen for suppressors of Drosophila NSF2 neuromuscular junction overgrowth (2005)
  • Author(s): Laviolette, M.J., Nunes, N., Peyre, J.B., Aigaki, T., Stewart, B.A.
  • Journal Title: Genetics 170 Pages: 779-792
• [Journal Article] Involvement of winged eye encoding a chromatin-associated bromo-adjacent homology domain protein in disc specification. (2005)
  • Author(s): Katsuyama, T., Sugawara, T., Tatsumi, M., Oshima, Y., Gehring, W., Aigaki, T., Kurata, S.
  • Journal Title: Proc. Natl. Acad. Sci. USA 102 Pages: 15918-15923
• [Journal Article] Gain-of-function screen identifies a role of the Sec61alpha translocon in Drosophila postmitotic neurotoxicity. (2005)
  • Author(s): Kanuka, H, Hiratou, T, Igaki, T, Kanda, H, Kuranaga, E, Sawamoto, K, Aigaki, T, Okano, H, Miura, M.
  • Journal Title: Biochim. Biophys. Acta 1726 Pages: 225-237
• [Journal Article] The RING finger-scaffold protein POSH targets TAK1 to control immunity signaling in Drosophila. (2005)
  • Author(s): Tsuda, M., Langmann, C., Harden, N., Aigaki, T.
  • Journal Title: EMBO reports 6 Pages: 1082-1087
• [Journal Article] Expression of a secreted form of Dally, a Drosophila glypican, induces overgrowth phenotype by affecting action range of Hedgehog. (2005)
  • Author(s): Takeo, S., Akiyama, T., Firkus, C., Aigaki, T., Nakato, H.
  • Journal Title: Dev. Biol. 284 Pages: 204-218
• [Journal Article] A Genetic screen for suppressors of Drosophila NSF2 neuromuscular junction overgrowth. (2005)
  • Author(s): Laviolette, M.J., Nunes, N., Peyre, J.B., Aigaki, T., Stewart, B.A.
  • Journal Title: Genetics 170 Pages: 779-792
• [Journal Article] The slender lobes gene, identified by retarded mushroom body development, is required for proper nucleolar organization in Drosophila (2005)
  • Author(s): Orihara-Ono, M., Suzuki, M., Saito, M., Yoda, Y., Aigaki, T., Hama, C.
  • Journal Title: Dev. Biol. 281 Pages: 121-133
• [Journal Article] Enhancer Trapping With a RedFluorescent Protein Reporter in Drosophila. (2005)
  • Author(s): Akimoto, A., Wada, H., Hayashi, S.
  • Journal Title: Developmental Dynamics 233 Pages: 993-997
• [Journal Article] The transmembrane protein, Tincar, is involved in the development of the compound eye in Drosophila melanogaster. (2005)
  • Author(s): Hirota, Y., Sawamoto, K., Takahashi, K., Ueda, R., Okano, H.
  • Journal Title: Dev. Genes Evol. 215 Pages: 90-96
• [Journal Article] Wing-to-leg homeosis by Spineless causes an apoptosis regulated by Fish-lips, a novel Leucine-Rich Repeats transmembrane protein. (2005)
  • Author(s): Adachi-Yamada, T., Harumoto, T., Sakurai, K., Ueda, R., Saigo, K., O'Connor, M.B., Nakato, H.
  • Journal Title: Mol. Cell. Biol. 25 Pages: 3140-3150
• [Journal Article] Inhibitor of apoptosis 2 and TAK1-binding protein are components of the Drosophila Imd pathway. (2005)
  • Author(s): Kleino, A., Valanne, S., Ulvila, J., Kallio, J., Myllymaki, H., Enwald, H., Stoven, S., Poidevin, M., Ueda, R., Hultmark, D., Lemaitre, B., Ramet, M.
  • Journal Title: The EMBO J. 24 Pages: 3423-3434
• [Journal Article] G-protein gamma subunit 1 is required for sugar reception in Drosophila. (2005)
  • Author(s): Ishimoto, H., Takahashi, K., Ueda, R., Tanimura, T.
  • Journal Title: The EMBO J. 24 Pages: 3259-3265
• [Journal Article] Involvement of winged eye encoding a chromatin-associated bromo-adjacent homology domain protein in disc specification. (2005)
  • Author(s): Katsuyama T., Sugawara, T., Tatsumi, M., Oshima, Y., Gehring, W., Aigaki, T., Kurata, S.
  • Journal Title: Proc.Natl.Acad.Sci.USA 102 Pages: 15918-15923
• [Journal Article] Gain-of-function screen identifies a role of the Sec61 alpha translocon in Drosophila postmitotic neurotoxicity. (2005)
  • Author(s): Kanuka, H, Hiratou, T, Igaki T, Kanda, H, Kuranaga, E, Sawamoto, K, Aigaki, T, Okano, H, Miura, M.
  • Journal Title: Biochim.Biophys.Acta 1726 Pages: 225-237
• [Journal Article] The RING finger-scaffold protein POSH targets TAK1 to control immunity signaling in Drosophila. (2005)
  • Author(s): Tsuda M., Langmann, C., Harden, N., Aigaki, T.
  • Journal Title: EMBO reports 6 Pages: 1082-1087
• [Journal Article] Expression of a secreted form of Dally, a Drosophila glypican, induces overgrowth phenotype by affecting action range of Hedgehog. (2005)
  • Author(s): Takeo, S., Akiyama, T., Firkus, C., Aigaki, T, Nakato, H.
  • Journal Title: Dev.Biol, 284 Pages: 204-218
• [Journal Article] A Genetic screen for suppressors of Drosophila NSF2 neuromuscular junction overgrowth. (2005)
  • Author(s): Laviolette M.J., Nunes, N., Peyre, J.B., Aigaki, T., Stewart, B.A.
  • Journal Title: Genetics 170 Pages: 779-792
• [Journal Article] A functional genomics strategy reveals clockwork orange as a transcriptional regulator in the Drosophila circadian clock
  • Author(s): Akira Matsumoto*, Maki Ukai-Tadenuma, Rikuhiro G. Yamada, Jerry Houl, Kenichiro D. Uno, Takeya Kasukawa, Brigitte Dauwalder, Taichi Q. Itoh, Kuniaki Takahashi, Ryu Ueda, Paul E. Hardin, Teiichi Tanimura, Hiroki R.
  • Journal Title: Genes and Development 21(13) Pages: 1687-1700
• [Journal Article] Drosophila orthologues of human b-secretase induce the secretion of a Golgi-resident transferase, heparan sulfate 6-O-sulfotransferase.
  • Author(s): Kotani, N., Kitazume, S., Kamimura, K., Takeo, S., Aigaki, T., Nakato, H., Hashimoto, Y.
  • Journal Title: J. Biochem. 137 Pages: 315-22
• [Presentation] ショウジョウバエゲノムの改変による寿命遺伝子の探索と解析 (2009)
  • Author(s): 相垣敏郎
  • Organizer: 日本基礎老化学会
  • Place of Presentation: 東京都健康長寿医療センター
  • Year and Date: 2009-10-31
• [Presentation] Mutations in Lipoyltrasferase(lipB) exhibits shorten life span and metabolic disorder in Drosophila. (2009)
  • Author(s): Tsuda, M., Y, Kishita. T, Kaneuchi, T, Aigaki
  • Organizer: JDRC.
  • Place of Presentation: 静岡
• [Presentation] Loss of lipoyltransferase (lipB) disrupts key metabolic pathways for energy production and leads to shortened lifespan in Drosophila. (2009)
  • Author(s): Kishita, Y., Tsuda, M., Aigaki, T.
  • Organizer: EDRC2009
  • Place of Presentation: Nice, France
• [Presentation] Drosophila sex-peptide: functional dissection and evolution. (2009)
  • Author(s): Tsuda, M., T, Aigaki
  • Organizer: 第32回日本分子生物学会
  • Place of Presentation: 横浜
• [Presentation] Role of laccase in cuticle sclerotization. (2009)
  • Author(s): T, Asano.J, Yatsu.K, Kusumoto.T, Aigaki
  • Organizer: 第9回日本ショウジョウバエ研究会
  • Place of Presentation: ヤマハリゾートつま恋
• [Presentation] Swagl, a novel gene tahat regulates body size in Drosophila. (2009)
  • Author(s): M, Funakoshi.K, Muramatsu.M, Tsuda.K, Hatsuta.S, Morishita., T, Aigaki
  • Organizer: 第9回日本ショウジョウバエ研究会
  • Place of Presentation: ヤマハリゾートつま恋
• [Presentation] In vitro binding assay of two odorant-binding proteins, OBP57d and OBP57e. (2009)
  • Author(s): E, Harada.T, Aigaki.T, Matsuo
  • Organizer: 第9回日本ショウジョウバエ研究会
  • Place of Presentation: ヤマハリゾートつま恋
• [Presentation] Inhibiting PKA activity in the mushroom bodies increases memory and may increase synaptic plasticity in Drosophila. (2009)
  • Author(s): J, Horiuchi.D, Yamazaki.T, Aigaki.M, Saitoe
  • Organizer: 第9回日本ショウジョウバエ研究会
  • Place of Presentation: ヤマハリゾートつま恋
• [Presentation] Identification of functionally important amino acids in two odorant-binding proteins, OBP57d and OBP57e. (2009)
  • Author(s): J, Nakagawa.T, Aigaki.T, Matsuo
  • Organizer: 第9回日本ショウジョウバエ研究会
  • Place of Presentation: ヤマハリゾートつま恋
• [Presentation] Conditional Knock-In Mutagenesis of Calcineurin Genes. (2009)
  • Author(s): Y, Nakai.M, Tsuda.T, Aigaki.
  • Organizer: 第9回日本ショウジョウバエ研究会
  • Place of Presentation: ヤマハリゾートつま恋
• [Presentation] Chemical genetics in Drosophila : development of a versatile culture system and an automated phenotype analysis for a high-throughput sample. (2009)
  • Author(s): Y, Sato.T, Kaneuchi.O, Masuda.K, Muramatsu.M, Tsuda.T, Aigaki
  • Organizer: 第9回日本ショウジョウバエ研究会
  • Place of Presentation: ヤマハリゾートつま恋
• [Presentation] Mutations in Lipoyltrasferase (lipB) exhibits shorten life span and metabolic disorder in Drosophila. (2009)
  • Author(s): S, Tomioka.J, Yasukawa.T, Aigaki.T, Matsuo
  • Organizer: 第9回日本ショウジョウバエ研究会
  • Place of Presentation: ヤマハリゾートつま恋
• [Presentation] Drosophila sex-peptide : functional dissection and evolution. (2009)
  • Author(s): 津田学 相垣敏郎
  • Organizer: 第32回日本分子生物学会
  • Place of Presentation: パシフィコ横浜
• [Presentation] Genes regulating body size, oxidative stress resistance and longevity in Drosophila (2008)
  • Author(s): Aigaki, T., et.al
  • Organizer: Korea-Japan joint symposium
  • Place of Presentation: Daegu, Korea
• [Presentation] Isoform-specific functions of BTB-Zn finger proteins encoded by lola in Drosophila (2008)
  • Author(s): Liesching, H., et.al
  • Organizer: International Conference of Genetics
  • Place of Presentation: Germany, Berlin
• [Presentation] ショウジョウバエの寿命と体の大きさを制御する新規遺伝子 (2008)
  • Author(s): 船越政史, 村松圭吾, 津田学, 初田浩志, 森下真一, 相垣敏郎
  • Organizer: 日本基礎老化学会第31回大会
  • Place of Presentation: 長野県松本市
• [Presentation] リボ酸転移酵素 (LipB) の機能抑制によるエネルギー代謝の変化と寿命延長効果 (2008)
  • Author(s): 木下善仁, 津田学, 相垣敏郎
  • Organizer: 日本基礎老化学会第31回大会
  • Place of Presentation: 長野県松本市
• [Presentation] Insulin Degrading Enzyme in Drosophila (2008)
  • Author(s): Kobayashi, T., Tsuda, M., Matsuo, T. and Aigaki, T
  • Organizer: 日本基礎老化学会第31回大会
  • Place of Presentation: 長野県松本市
• [Presentation] Role of Thioredoxin genes in longevity determination in Drosophila (2008)
  • Author(s): Tsuda, M. and Aigaki, T
  • Organizer: The Japan-Korea Joint Seminar
  • Place of Presentation: Nagasaki
• [Presentation] ショウジョウバエD-WIP機能破壊変異体の作製と表現型の解析 (2008)
  • Author(s): 宮田直政, 津田学, 家村俊一郎, 夏目徹, 相垣敏郎
  • Organizer: 第31回日本分子生物学会
  • Place of Presentation: 兵庫県神戸市
• [Presentation] ショウジョウバエのmRNA転写産物の5末端配列データベース (2008)
  • Author(s): Budrul Ahsan, 橋本真一, 村松圭吾, 津田学, 佐々木惇, 齊藤太郎, 松島綱治, 相垣敏郎, 森下真一
  • Organizer: 第31回日本分子生物学会
  • Place of Presentation: 兵庫県神戸市
• [Presentation] ショウジョウバエ寿命決定におけるチオレドキシン遺伝子の機能 (2008)
  • Author(s): 津田学, 大高領介, 相垣敏郎
  • Organizer: 第31回日本分子生物学会
  • Place of Presentation: 兵庫県神戸市
• [Presentation] Structures and functional properties of P{GS} vectors and insertions (2008)
  • Author(s): Aigaki T
  • Organizer: 20th European Drosophila Research Conference
  • Place of Presentation: Vienna,Austria
• [Book] 老化・老年病研究のため動 物実験ガイドブック72-77ショウジョウバエモデル(日本基礎老化学) (2008)
  • Author(s): 相垣敏郎
  • Publisher: 丸善
• [Book] 考化・老年病研究のための動物実験ガイドブック (日本基礎老化学会編) ショウジョウバエモデル (2008)
  • Author(s): 相垣敏郎
  • Publisher: アドスリー丸善
• [Remarks] GSベクター挿入系統のマップ情報、挿入サイトのゲノム配列、近傍の遺伝子、強制発現される遺伝子、特定のGAL4を使って強制発現を誘導した時に生じる表現型情報が登録されている。
  • URL: http://gsdb.biol.metro-u.ac.jp/%7Edclust/
• [Remarks] GS系統データベース
  • URL: http://gsdb.biol.metro-u.ac.jp/%7Eddust/
• [Remarks] RNAi系統データベース
  • URL: http://www.shigen.nig.ac.jp/fly/nigfly/index.jsp
• [Patent(Industrial Property Rights)] 生物用小型8連プラスチックセル (2006)
  • Inventor(s): 相垣 敏郎
  • Industrial Property Rights Holder: 首都大学東京
  • Filing Date: 2006
• [Patent(Industrial Property Rights)] 遺伝子探索ベクター隣接ゲノム配列の同定方法、及び酸化ストレス感受性変異体 (2005)
  • Inventor(s): 相垣敏郎
  • Industrial Property Rights Holder: 首都大学東京
  • Filing Date: 2005-11-09
• [Patent(Industrial Property Rights)] 遺伝子探索ベクター隣接ゲノム配列の同定方法、及び酸化ストレス感受性変異体 (2005)
  • Inventor(s): 相垣 敏郎
  • Industrial Property Rights Holder: 首都大学東京
  • Industrial Property Number: 2005-324980
  • Filing Date: 2005-11-09