| Project/Area Number |
17018016
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| Research Category |
Grant-in-Aid for Scientific Research on Priority Areas
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| Allocation Type | Single-year Grants |
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| Review Section |
Biological Sciences
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| Research Institution | Nagoya University |
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Principal Investigator |
HORI Hiroshi Nagoya University, 大学院・理学研究科, 名誉教授 (60116663)
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| Co-Investigator(Kenkyū-buntansha) |
MITANI Hiroshi 東京大学, 大学院・新領域創成科学研究科, 教授 (70181922)
SASAKI Takashi 慶応大学, 医学部, 助手 (70306843)
SHIMIZU Atsushi 慶応大学, 医学部, 助手 (30327655)
KANAMORI Akira 名古屋大学, 大学院・理学研究科, 助教 (40324389)
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| Project Period (FY) |
2005 – 2009
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| Project Status |
Completed (Fiscal Year 2009)
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| Budget Amount *help |
¥93,500,000 (Direct Cost: ¥93,500,000)
Fiscal Year 2009: ¥18,000,000 (Direct Cost: ¥18,000,000)
Fiscal Year 2008: ¥18,000,000 (Direct Cost: ¥18,000,000)
Fiscal Year 2007: ¥18,800,000 (Direct Cost: ¥18,800,000)
Fiscal Year 2006: ¥18,700,000 (Direct Cost: ¥18,700,000)
Fiscal Year 2005: ¥20,000,000 (Direct Cost: ¥20,000,000)
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| Keywords | メダカ / ゲノム / BACライブラリー / 染色体再編 / 比較ゲノム |
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| Research Abstract |
Evolution of the genome size in vertebrates is often affected by changes in the noncoding sequences, for which insertions and deletions (indels) of small nucleotide sequences and amplification of repetitive elements are considered responsible. In this project, we especially compared the genomic DNA sequences of two kinds of fish, medaka (Oryzias latipes) and fugu (Takifugu rubripes), which show two-fold difference in the genome size (800 Mb vs. 400 Mb). We determined a contiguous DNA sequence of 790 kb from the medaka chromosome LG22 (linkage group 22). The sequence was aligned common between two fishes, and were found abundant in various repetitive elements including many types of unclassified low copy repeats, all of which accounted for more than a half (54%) of the genome size difference. These results strongly indicated that amplification of repetitive elements and compilation of indels are major driving forces to facilitate changes in the genome size.
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