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Functional genomics using transgenic zebrafish

Research Project

Project/Area Number 17018034
Research Category

Grant-in-Aid for Scientific Research on Priority Areas

Allocation TypeSingle-year Grants
Review Section Biological Sciences
Research InstitutionNational Institute of Genetics

Principal Investigator

KAWAKAMI Koichi  National Institute of Genetics, 個体遺伝研究系, 教授 (70195048)

Project Period (FY) 2005 – 2009
Project Status Completed (Fiscal Year 2009)
Budget Amount *help
¥70,000,000 (Direct Cost: ¥70,000,000)
Fiscal Year 2009: ¥13,500,000 (Direct Cost: ¥13,500,000)
Fiscal Year 2008: ¥13,500,000 (Direct Cost: ¥13,500,000)
Fiscal Year 2007: ¥13,700,000 (Direct Cost: ¥13,700,000)
Fiscal Year 2006: ¥13,600,000 (Direct Cost: ¥13,600,000)
Fiscal Year 2005: ¥15,700,000 (Direct Cost: ¥15,700,000)
Keywords動物ゲノム / ゲノム機能発現 / 遺伝子発現調節 / ゲノムデータベース / 分子生物学 / 発生遺伝学 / ゼブラフィッシュ / トランスポゾン / 挿入変異 / エンハンサートラップ / 遺伝子トラップ / トランスジェニック動物 / セブラフィッシュ / 転移酵素 / トランスジェニックフィッシュ / インサーショナルミュータジェネシス / 発生 / 器官形成
Research Abstract

We successfully developed novel genetic methods by using the Tol2 transposon system such as gene trapping, enhancer trapping, the Gal4-UAS system and in vivo transposition system. By utilizing these methods, we created more than 600 of transgenic fish lines that express GFP or Gal4 in specific tissues, cells and organs. By analyzing these transgenic fish, we studied the function of important developmental genes. Further, we constructed a database of the transgenic fish resource and facilitated collaboration with researchers all over the world. Thus, our achievements greatly contributed advances of the study of vertebrate genes.

Report

(6 results)
  • 2009 Annual Research Report   Final Research Report ( PDF )
  • 2008 Annual Research Report
  • 2007 Annual Research Report
  • 2006 Annual Research Report
  • 2005 Annual Research Report
  • Research Products

    (198 results)

All 2010 2009 2008 2007 2006 2005 Other

All Journal Article (81 results) (of which Peer Reviewed: 38 results) Presentation (107 results) Book (3 results) Remarks (6 results) Patent(Industrial Property Rights) (1 results)

  • [Journal Article] Mys Protein Regulates Protein Kinase A Activity by Interacting with Regulatory Type IαSubunit during Vertebrate Development.2010

    • Author(s)
      Kotani, T., Iemura, S.I., Natsume, T., Kawakami, K., Yamashita, M.
    • Journal Title

      The Journal of Biological Chemistry 285(in press)

      Pages: 5106-5116

    • Related Report
      2009 Final Research Report
  • [Journal Article] Development of the circadian oscillator during differentiation of mouse embryonic stem cells in vitro2010

    • Author(s)
      Yagita, K.
    • Journal Title

      Proc.Natl.Acad.Sci.USA 107

      Pages: 3846-3851

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Efficient transient rescue of hematopoietic mutant phenotypes in zebrafish using Tol2-mediated transgenesis2010

    • Author(s)
      Takeuchi, M.
    • Journal Title

      Development Growth & Differentiation 52

      Pages: 245-250

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Mys Protein Regulates Protein Kinase A Activity by Interacting with Regulatory Type I α Subunit during Vertebrate Development2010

    • Author(s)
      Kotani, T.
    • Journal Title

      The Journal of Biological Chemistry 285

      Pages: 5106-5116

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Real-time monitoring of circadian clock oscillations in primary cultures of mammalian cells using Tol2 transposon-mediated gene transfer strategy2010

    • Author(s)
      Yagita, K.
    • Journal Title

      BMC Biotechnology 10(1)

      Pages: 3-3

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Interaction with surrounding normal epithelial cells influences signalling pathways and behaviour of Src-transformed cells2010

    • Author(s)
      Kajita, M.
    • Journal Title

      Journal of Cell Science 123

      Pages: 171-180

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Sleep-wake regulation and hypocretin-melatonin interaction in zebrafish.2009

    • Author(s)
      Appelbaum, L., Wang, G.X., Maro, G.S., Mori, R., Tovin, A., Marin, W., Yokogawa, T., Kawakami, K., Smith, S.J., Gothilf, Y., Mignot, E., Mourrain, P.
    • Journal Title

      Proc. Natl. Acad. Sci. USA 106

      Pages: 21942-21947

    • Related Report
      2009 Final Research Report
  • [Journal Article] Illuminating cell-cycle progression in the developing zebrafish embryo.2009

    • Author(s)
      Sugiyama, M., Sakaue-Sawano, A., Iimura, T., Fukami, K., Kitaguchi, T., Kawakami, K., Okamoto, H., Higashijima, SI., Miyawaki, A.
    • Journal Title

      Proc. Natl. Acad. Sci. USA 106

      Pages: 20812-20817

    • Related Report
      2009 Final Research Report
  • [Journal Article] A novel conserved evx1 enhancer links spinal interneuron morphology and cis-regulation from fish to mammals.2009

    • Author(s)
      Suster, M.L., Kania, A., Liao, M., Asakawa, K., Charron, F., Kawakami, K., Drapeau, P.
    • Journal Title

      Developmental Biology 325

      Pages: 422-433

    • Related Report
      2009 Final Research Report
  • [Journal Article] Afferent neurons of the zebrafish lateral line are strict selectors of hair-cell orientation.2009

    • Author(s)
      Faucherre, A., Pujol-Marti J., Kawakami, K., Lopez-Schier, H.
    • Journal Title

      PLoS One 4

    • Related Report
      2009 Final Research Report
  • [Journal Article] Mechanism of development of ionocytes rich in vacuolar-type H(+)-ATPase in the skin of zebrafish larvae.2009

    • Author(s)
      Esaki, M., Hoshijima, K., Nakamura, N., Munakata, K., Tanaka, M., Ookata, K., Asakawa, K., Kawakami, K., Wang, W., Weinberg, E.S., Hirose, S.
    • Journal Title

      Developmental Biology 329

      Pages: 116-129

    • Related Report
      2009 Final Research Report
  • [Journal Article] Olfactory neural circuitry for attraction to amino acids revealed by transposon-mediated gene trap approach in zebrafish2009

    • Author(s)
      Koide, T., Miyasaka, N., Morimoto, K., Asakawa, K., Urasaki, A., Kawakami, K., Yoshihara, Y.
    • Journal Title

      Proc. Natl. Acad. Sci. USA 106

      Pages: 9884-9889

    • Related Report
      2009 Final Research Report
  • [Journal Article] Dynamic coupling of pattern formation and morphogenesis in the developing vertebrate retina2009

    • Author(s)
      Picker, A., Cavodeassi, F., Machate, A., Bernauer, S., Hans, S., Abe, G., Kawakami, K., Wilson, S.W., Brand, M.
    • Journal Title

      PLoS Biology 7(10)

    • Related Report
      2009 Final Research Report
  • [Journal Article] Transposon-mediated BAC transgenesis in zebrafish and mice.2009

    • Author(s)
      Suster, M.L., Sumiyama, K., Kawakami, K.
    • Journal Title

      BMC Genomics 10

      Pages: 477-477

    • Related Report
      2009 Final Research Report
  • [Journal Article] The Tol2-mediated Gal4-UAS method for gene and enhancer trapping in zebrafish.2009

    • Author(s)
      Asakawa, K., Kawakami, K.
    • Journal Title

      Methods 49(3)

      Pages: 275-281

    • Related Report
      2009 Final Research Report
  • [Journal Article] Cis-regulation and chromosomal rearrangement of the fgf8 locus after the teleost/tetrapod split2009

    • Author(s)
      Komisarczuk, A.Z., Kawakami, K., Becker, T.S.
    • Journal Title

      Developmental Biology 336(2)

      Pages: 301-312

    • Related Report
      2009 Final Research Report
  • [Journal Article] Sleep-wake regulation and hypocretin-melatonin interaction in zebrafish2009

    • Author(s)
      Appelbaum, L.
    • Journal Title

      Proc.Natl.Acad.Sci.USA 106

      Pages: 21942-21947

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Illuminating cell-cycle progression in the developing zebrafish embryo2009

    • Author(s)
      Sugiyama, M.
    • Journal Title

      Proc.Natl.Acad.Sci.USA 106

      Pages: 20812-20817

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] The transgensis and gene and enhancer trap methods in zebrafish by using the Tol2 transposable element2009

    • Author(s)
      Kawakami, K.
    • Journal Title

      Essential zebrafish methods : genetics and genomics

      Pages: 153-173

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Cis-regulation and chromosomal rearrangement of the fgf8 locus after the teleost/tetrapod split2009

    • Author(s)
      Komisarczuk, A.Z.
    • Journal Title

      Developmental Biology 336(2)

      Pages: 301-312

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] The Tol2-mediated Ga14-UAS method for gene and enhancer trapping in zebrafish2009

    • Author(s)
      Asakawa, K.
    • Journal Title

      Methods 49(3)

      Pages: 275-281

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Transposon-mediated BAC transgenesis in zebrafish and mice2009

    • Author(s)
      Suster, ML.
    • Journal Title

      BMC Genomics 16;10

      Pages: 477-477

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Dynamic coupling of pattern formation and morphogenesis in the developing vertebrate retina2009

    • Author(s)
      Picker, A.
    • Journal Title

      PLoS Biol 7(10)

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Estrogen-responsive transient expression assay using a brain aromatase-based reporter gene in zebrafish(Danio rerio)2009

    • Author(s)
      Kim, DJ.
    • Journal Title

      Comp Med 59(5)

      Pages: 416-423

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A combination of transposable elements and magnetic cell sorting provides a very efficient transgenesis system for chicken primary erythroid progenitors2009

    • Author(s)
      Mejia-Pous, C.
    • Journal Title

      BMC Biotechnology 18;9(1)

      Pages: 81-81

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Developmental toxicity and brain aromatase induction by high genistein concentrations in zebrafish embryos2009

    • Author(s)
      Kim, DJ.
    • Journal Title

      Toxicology Mechanisms and Methods 19(3)

      Pages: 251-256

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Olfactory neural circuitry for attraction to amino acids revealed by transposon-mediated gene trap approach in zebrafish2009

    • Author(s)
      Koide, T.
    • Journal Title

      Proc.Natl.Acad.Sci.USA 106

      Pages: 9884-9889

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Transgenesis in zebrafish with the tol2 transposon system2009

    • Author(s)
      Suster, ML.
    • Journal Title

      Methods in Molecular Biology 561

      Pages: 41-63

    • Related Report
      2009 Annual Research Report
  • [Journal Article] Transcriptional regulation of a myeloid-lineage specific gene lysozyme C during zebrafish myelopoiesis2009

    • Author(s)
      Kitaguchi, T.
    • Journal Title

      Mechanisms of Development 126

      Pages: 314-323

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Benomyl induction of brain aromatase and toxic effects in the zebrafish embryo2009

    • Author(s)
      Kim, DJ.
    • Journal Title

      Journal of Applied Toxicology 29(4)

      Pages: 289-294

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Analysis of genes and genome by the tol2-mediated gene and enhancer trap methods2009

    • Author(s)
      浦崎明宏
    • Journal Title

      Methods in Molecular Biology 546

      Pages: 85-102

    • Related Report
      2009 Annual Research Report
  • [Journal Article] Transient and stable transgenesis using tol2 transposon vectors2009

    • Author(s)
      Kikuta, H.
    • Journal Title

      Methods in Molecular Biology 546

      Pages: 69-84

    • Related Report
      2009 Annual Research Report
  • [Journal Article] Mechanism of development of ionocytes rich in vacuolar-typeH(+)-ATPase in the skin of zebrafish larvae2009

    • Author(s)
      Esaki, M.
    • Journal Title

      Developmental Biology 329

      Pages: 116-129

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] メダカトランスポゾンTbl2が開く新しいゼブラフィッシュ研究2009

    • Author(s)
      浦崎明宏
    • Journal Title

      細胞工学 28

      Pages: 586-591

    • Related Report
      2009 Annual Research Report
  • [Journal Article] Recapitulation of zebrafish sncga expression pattern and labeling the habenular complex in transgenic zebrafish using green fluorescent protein reporter gene2009

    • Author(s)
      Chen, YC.
    • Journal Title

      Developmental Dynamics 238

      Pages: 746-754

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Afferent neurons of the zebrafish lateral line are strict selectors of hair-cell orientation2009

    • Author(s)
      Faucherre, A.
    • Journal Title

      PLoS ONE. 4

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A novel conserved evx1 enhancer links spinal interneuron morphology and cis-regulation from fish to mammals2009

    • Author(s)
      Suster, ML.
    • Journal Title

      Developmental Biology 325

      Pages: 422-433

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Expression of H-RASV12 in a zebrafish model of Costellosyndrome causes cellular senescence in adult proliferating cells2009

    • Author(s)
      Santoriello, C.
    • Journal Title

      Disease models & Mechanisms 2

      Pages: 56-57

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Insertional mutagenesis by the Tol2 transposon-mediated enhancer trap approach generated mutations in two developmental genes: tcf7 and synembryn-like.2008

    • Author(s)
      Nagayoshi, S., Hayashi, E., Abe, G., Osato, N., Asakawa, K., Urasaki, A., Horikawa, K., Ikeo, K., Takeda, H., Kawakami, K.
    • Journal Title

      Development 135

      Pages: 159-169

    • Related Report
      2009 Final Research Report
  • [Journal Article] Genetic dissection of neural circuits by Tol2 transposon-mediated Gal4 gene and enhancer trapping in zebrafish2008

    • Author(s)
      Asakawa, K., Suster, M.L., Mizusawa, K., Nagayoshi, S., Kotani, T., Urasaki, A., Kishimoto, Y., Hibi, M., Kawakami, K.
    • Journal Title

      Proc. Natl. Acad. Sci. USA 105

      Pages: 1255-1260

    • Related Report
      2009 Final Research Report
  • [Journal Article] misty somites, a maternal effect gene identified by transposon-mediated insertional mutagenesis in zebrafish that is essential for the somite boundary maintenance.2008

    • Author(s)
      Kotani, T., Kawakami, K.
    • Journal Title

      Developmental Biology 316

      Pages: 383-396

    • Related Report
      2009 Final Research Report
  • [Journal Article] Targeted gene expression by the Gal4-UAS system in zebrafish2008

    • Author(s)
      Asakawa, K., Kawakami, K.
    • Journal Title

      Development Growth & Differentiation 50

      Pages: 391-399

    • NAID

      10021096914

    • Related Report
      2009 Final Research Report
  • [Journal Article] Efficient transposition of the Tol2 transposable element from a single-copy donor in zebrafish2008

    • Author(s)
      Urasaki, A., Asakawa, K., Kawakami, K.
    • Journal Title

      Proc. Natl. Acad. Sci. USA 105

      Pages: 19827-19832

    • Related Report
      2009 Final Research Report
  • [Journal Article] Efficient transposition of the Tol2 transposable element from a single-copy donor in zebrafish2008

    • Author(s)
      Urasaki, A.
    • Journal Title

      Proc. Natl. Acad. Sci. USA 105

      Pages: 19827-19832

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Tracing retinal fiber trajectory with a method of transposon-mediated genomic integration in chick embryo2008

    • Author(s)
      Harada, H.
    • Journal Title

      Development Growth & Differentiation 50

      Pages: 697-702

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Transposition of the vertebrate Tol2 transposable element in Drosophila melanogaster2008

    • Author(s)
      Urasaki, A.
    • Journal Title

      Gene 425

      Pages: 64-68

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] G2R Cre reporter transgenic zebrafish2008

    • Author(s)
      Yoshikawa, S.
    • Journal Title

      Developmental Dynamics 237

      Pages: 2460-2465

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Views on four key questions about zebrafish research2008

    • Author(s)
      Alestrom, P.
    • Journal Title

      Zebrafish 5

      Pages: 9-24

    • Related Report
      2008 Annual Research Report
  • [Journal Article] Targeted gene expression by the Ga14-UAS system in zebrafish2008

    • Author(s)
      Asakawa K.
    • Journal Title

      Development, Growth & Differentiation 50

      Pages: 391-399

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Transposon-mediated stable integration and tetracycline-inducible expression of electroporated transgenes in chicken embryos2008

    • Author(s)
      Takahashi, Y.
    • Journal Title

      Methods in Cell Biology 87

      Pages: 271-280

    • Related Report
      2008 Annual Research Report
  • [Journal Article] misty somites, a maternal effect gene identified by transposon-mediated insertional mutagenesis in zebrafish that is essential for the somite boundary maintenance2008

    • Author(s)
      Kotani, T.
    • Journal Title

      Development Biology 316

      Pages: 383-396

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Genetic dissection of neural circuits by To12 transposon-mediated Gal4 gene and enhancer trapping in zebrafish2008

    • Author(s)
      Asakawa, K.
    • Journal Title

      Proc.Natl.Acad.Sci.USA 105

      Pages: 1255-1260

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Insertional mutagenesis by the To12 transposon-mediated enhancer trap approach generated mutations in two developmental genes : tcf7 and synembryn-like2008

    • Author(s)
      Nagayoshi, S.
    • Journal Title

      Development 135

      Pages: 159-169

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Patterning the zebrafish diencephalon by the conserved zinc-finger protein Fezl.2007

    • Author(s)
      Jeong, J. -Y., Einhorn, Z., Mathur, P., Chen, L., Lee, S., Kawakami, K., Guo, S.
    • Journal Title

      Development 134

      Pages: 127-136

    • Related Report
      2009 Final Research Report
  • [Journal Article] Targeting neural circuitry in zebrafish using GAL4 enhancer trapping.2007

    • Author(s)
      Scott, E.K., Mason, L., Arrenberg, A.B., Ziv, L., Gosse, N.J., Xiao, T., Chi, N.C., Asakawa, K., Kawakami, K., Baier, H.
    • Journal Title

      Nature Methods 4

      Pages: 323-326

    • Related Report
      2009 Final Research Report
  • [Journal Article] Tol2: a versatile gene transfer vector in vertebrates2007

    • Author(s)
      Kawakami, K.
    • Journal Title

      Genome Biology 8(Suppl1)

    • Related Report
      2009 Final Research Report
  • [Journal Article] To12 : a versatile gene transfer vector in vertebrates2007

    • Author(s)
      Kawakami, K.
    • Journal Title

      Genome Biology 8

      Pages: 7-7

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Nodal signals mediate interactions between the extra-embryonic and embryonic tissues in zebrafish.2007

    • Author(s)
      Fan, X.
    • Journal Title

      Developmental Biology 310

      Pages: 363-378

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A cardiac myosin light chain kinase regulates sarcomere assembly in the vertebrate heart.2007

    • Author(s)
      Seguchi, 0.
    • Journal Title

      The Journal of Clinical Investigation 117

      Pages: 2812-2824

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Localization of ammonia transporter Rhcg1 in mitochondrion-rich cells of yolk sac, gill, and kidney of zebrafish and its ionic strength-dependent expression.2007

    • Author(s)
      Nakada, T.
    • Journal Title

      Am J Physiol Regul Integr Comp Physiol 293

      Pages: 1743-1753

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Recombinant To12 transposase with activity in Xenopus embryos.2007

    • Author(s)
      Shibano, T.
    • Journal Title

      FEBS letters 581

      Pages: 4333-4336

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] stable integration and conditional expression of electroporated transgenes in chicken embryos2007

    • Author(s)
      Sato, Y.
    • Journal Title

      Developmental Biology 305

      Pages: 616-624

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Targeting neural circuitry in zebrafish using GAL4 enhancer trapping2007

    • Author(s)
      Scott, E.K.
    • Journal Title

      Nature Methods 48

      Pages: 323-326

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 脊椎動物におけるトランスポゾンを用いた遺伝学的方法論2007

    • Author(s)
      浦崎明宏
    • Journal Title

      実験医学 25

      Pages: 2507-2512

    • Related Report
      2007 Annual Research Report
  • [Journal Article] To12トランスポゾンを用いたゼブラフィッシュGaL4エンハンサートラップ法の確立2007

    • Author(s)
      浅川和秀
    • Journal Title

      バイオテクノロジージャーナル 7

      Pages: 603-606

    • Related Report
      2007 Annual Research Report
  • [Journal Article] Patterning the zebrafish diencephalon by the conserved zinc-finger protein Fezl.2007

    • Author(s)
      Jae-Yeon Jeong
    • Journal Title

      Development 134

      Pages: 127-136

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Visualization in zebrafish larvae of Na+ uptake in mitochondrion-rich cells whose differentiation is dependent on foxi3a2007

    • Author(s)
      Masahiro Esaki
    • Journal Title

      American Journal of Physiology : Regulatory Integrative and Comparative Physiology 292

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Spatiotemporal localization of germ plasm RNAs during zebrafish oogenesis.2007

    • Author(s)
      Kosaka, K.
    • Journal Title

      Mechanism of Development 124

      Pages: 279-289

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Transposon-mediated gene trapping in zebrafish2006

    • Author(s)
      Kotani, T., Nagayoshi, S., Urasaki, A., Kawakami, K.
    • Journal Title

      Methods 39

      Pages: 199-206

    • Related Report
      2009 Final Research Report
  • [Journal Article] Functional dissection of the Tol2 transposable element identified the minimal cis-sequence and a highly repetitive sequence in the subterminal region essential for transposition.2006

    • Author(s)
      Urasaki, A., Morvan, G., Kawakami, K.
    • Journal Title

      Genetics 174

      Pages: 639-649

    • Related Report
      2009 Final Research Report
  • [Journal Article] Evaluating the biological relevance of putative enhancers using Tol2 transposon-mediated transgenesis in zebrafish.2006

    • Author(s)
      Fisher, S., Grice, E.A., Vinton, R.M., Bessling, S.L., Urasaki, A., Kawakami, K., McCallion, A.S.
    • Journal Title

      Nature protocols 1

      Pages: 1297-1305

    • Related Report
      2009 Final Research Report
  • [Journal Article] Genomic organization, alternative splicing, and multiple regulatory regions of the zebrafish fgf8 gene.2006

    • Author(s)
      Fumitaka Inoue
    • Journal Title

      Development Growth & Differentiation 48

      Pages: 447-462

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Tol2 transposon mediated transgenesis in Xenopus tropicalis.2006

    • Author(s)
      Michelle R.Johnson Hamlet
    • Journal Title

      Genesis 44

      Pages: 438-445

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Cadherin is required for dendritic morphogenesis and synaptic terminal organization of retinal horizontal cells.2006

    • Author(s)
      Koji Tanabe
    • Journal Title

      Development 133

      Pages: 4085-4096

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Functional dissection of the Tol2 transposable element identified the minimal cis-sequence and a highly repetitive sequence in the subterminal region essential for transposition.2006

    • Author(s)
      Akihiro Urasaki
    • Journal Title

      Genetics 174

      Pages: 639-649

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Evaluating the biological relevance of putative enhancers using Tol2 transposon-mediated transgenesis in zebrafish.2006

    • Author(s)
      Shannon Fisher
    • Journal Title

      Nature protocols 1

      Pages: 1297-1305

    • Related Report
      2006 Annual Research Report
  • [Journal Article] The Fugu tyrp1 promoter directs specific GFP expression in zebrafish : tools to study the RPE and the neural crest-derived melanophores.2006

    • Author(s)
      Zou, J.
    • Journal Title

      Pigment Cell Research 19

      Pages: 615-627

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Migration of zebrafish primordial germ cells : a role for Myosin contraction and cytoplasmic flow.2006

    • Author(s)
      Blaser, H.
    • Journal Title

      Developmental Cell 11

      Pages: 613-627

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Transposon tools and methods in zebrafish2005

    • Author(s)
      Kawakami, K.
    • Journal Title

      Developmental Dynamics 234

      Pages: 244-254

    • Related Report
      2009 Final Research Report
  • [Journal Article] Transposon tools and methods in zebrafish2005

    • Author(s)
      Koichi Kawakami
    • Journal Title

      Developmental Dynamics 234

      Pages: 244-254

    • Related Report
      2005 Annual Research Report
  • [Journal Article] The zebrafish pob gene encodes a novel protein required for survival of red cone photoreceptor cells2005

    • Author(s)
      Taylor, M.R., Kikkawa, S., Diez-Juan, A., Ramamurthy, V., Kawakami K..Carmeliet, P., Brockerhoff, S.E.
    • Journal Title

      Genetics 170

      Pages: 263-273

    • Related Report
      2005 Annual Research Report
  • [Presentation] From gene to function: a quest with a transposon.2010

    • Author(s)
      Kawakami, K.
    • Organizer
      Zebrafish 2010: 11th Australia & New Zealand Workshop.
    • Place of Presentation
      Sydney. Australia
    • Related Report
      2009 Final Research Report
  • [Presentation] From gene to function : a quest with a transposon2010

    • Author(s)
      川上浩一
    • Organizer
      zebrafish 2010 : 11th Australia & New Zealand Workshop
    • Place of Presentation
      Sydney
    • Related Report
      2009 Annual Research Report
  • [Presentation] 光るゼブラフィッシュリソース2009

    • Author(s)
      川上浩一
    • Organizer
      NBRPシンポジウム「NBRPが提供するミュータントリソース」
    • Place of Presentation
      横浜
    • Year and Date
      2009-12-09
    • Related Report
      2009 Annual Research Report
  • [Presentation] ゼブラフィッシュ胸鰭末端上皮の形態形成2009

    • Author(s)
      矢野十織
    • Organizer
      第6回 東北大学バイオサイエンスシンポジウム
    • Place of Presentation
      仙台
    • Year and Date
      2009-06-16
    • Related Report
      2009 Annual Research Report
  • [Presentation] zTrap : zebrafish gene trap and enhancer trap database2009

    • Author(s)
      川上浩一
    • Organizer
      データベースが拓くこれからのライフサイエンス
    • Place of Presentation
      東京
    • Year and Date
      2009-06-12
    • Related Report
      2009 Annual Research Report
  • [Presentation] Transposon-mediated gene trapping, enhancer trapping and insertional mutagenesis2009

    • Author(s)
      Kawakami, K.
    • Organizer
      The 4th Asia-Oceania Zebrafish Meeting.
    • Place of Presentation
      Jeju, Korea
    • Related Report
      2009 Final Research Report
  • [Presentation] Tol2-mediated transgenesis in zebrafish and mice.2009

    • Author(s)
      Kawakami, K.
    • Organizer
      Conference on Genome Engineering.
    • Place of Presentation
      Minneapolis, Minnesota
    • Related Report
      2009 Final Research Report
  • [Presentation] Transposon-mediated insertional mutagenesis: an overview.2009

    • Author(s)
      Kawakami, K.
    • Organizer
      Zebrafish Knockout Project: Organization & Strategy Meeting
    • Place of Presentation
      Cambridge, UK.
    • Related Report
      2009 Final Research Report
  • [Presentation] Recent advances in the Tol2 transposon technology in zebrafish.2009

    • Author(s)
      Kawakami, K.
    • Organizer
      3rd Strategic Conference of Zebrafish Investigators.
    • Place of Presentation
      Asilomar, California.
    • Related Report
      2009 Final Research Report
  • [Presentation] Genetic methods using the Tol2 transposable element in zebrafish2009

    • Author(s)
      川上浩一
    • Organizer
      International Symposium on Marine Genomics 2009
    • Place of Presentation
      Naha, Okinawa
    • Related Report
      2009 Annual Research Report
  • [Presentation] Role of the hindbrain in embyonic motility and location2009

    • Author(s)
      浅川和秀
    • Organizer
      第32回日本分子生物学会年会
    • Place of Presentation
      横浜
    • Related Report
      2009 Annual Research Report
  • [Presentation] Jagged-Notch signaling regulates patterning activity and structural role of the Notochord through control of cell differentiation2009

    • Author(s)
      Morita, R.
    • Organizer
      第32回日本分子生物学会年会
    • Place of Presentation
      横浜
    • Related Report
      2009 Annual Research Report
  • [Presentation] Mechanism of pectoral fin development in zebrafish using//prx1//limb-specific enhancer activity2009

    • Author(s)
      矢野十織
    • Organizer
      第32回日本分子生物学会年会
    • Place of Presentation
      横浜
    • Related Report
      2009 Annual Research Report
  • [Presentation] Gene trap revealed a roll of Sox5 in the morphogenesis of the semicircular canal2009

    • Author(s)
      Tanino, S.
    • Organizer
      第32回日本分子生物学会年会
    • Place of Presentation
      横浜
    • Related Report
      2009 Annual Research Report
  • [Presentation] An easy and effective approach to rescue zebrafish mutant phenotypes via transient transgenesis using Tol2 constructs2009

    • Author(s)
      竹内未紀
    • Organizer
      第32回日本分子生物学会年会
    • Place of Presentation
      横浜
    • Related Report
      2009 Annual Research Report
  • [Presentation] Genetic inactivation of the habenulo-interpeduncular projection enhances the conditioned fear response in zebrafish2009

    • Author(s)
      揚妻正和
    • Organizer
      第32回日本分子生物学会年会
    • Place of Presentation
      横浜
    • Related Report
      2009 Annual Research Report
  • [Presentation] Insertional mutagenesis of the mouse ES cell genome with the Tol2 transposon2009

    • Author(s)
      吉田綾子
    • Organizer
      第32回日本分子生物学会年会
    • Place of Presentation
      横浜
    • Related Report
      2009 Annual Research Report
  • [Presentation] Tol2を用いたゼブラフィッシュ遺伝子の網羅的破壊2009

    • Author(s)
      浦崎明宏
    • Organizer
      日本遺伝学会第81回大会
    • Place of Presentation
      松本
    • Related Report
      2009 Annual Research Report
  • [Presentation] 手綱核・脚間核路の遺伝学的破壊はゼブラフィッシュの恐怖反応を昂進させる2009

    • Author(s)
      揚妻正和
    • Organizer
      第32回日本神経科学大会
    • Place of Presentation
      名古屋
    • Related Report
      2009 Annual Research Report
  • [Presentation] カルシウムイメージング法を用いたゼブラフィッシュ機能的神経回路の可視化2009

    • Author(s)
      武藤彩
    • Organizer
      第32回日本神経科学大会
    • Place of Presentation
      名古屋
    • Related Report
      2009 Annual Research Report
  • [Presentation] アミノ酸への誘因行動を司るゼブラフィッシュ嗅覚神経回路の遺伝学的解析2009

    • Author(s)
      小出哲也
    • Organizer
      第32回日本神経科学大会
    • Place of Presentation
      名古屋
    • Related Report
      2009 Annual Research Report
  • [Presentation] 子宮内穿孔法とTol2トランスポゾンシステムを用いてグリア細胞特異的に遺伝子を発現させる方法の開発2009

    • Author(s)
      吉田綾子
    • Organizer
      第32回日本神経科学大会
    • Place of Presentation
      名古屋
    • Related Report
      2009 Annual Research Report
  • [Presentation] 小脳を構成するニューロンとグリアの発生-子宮内電気穿孔法を用いた網羅的解析-2009

    • Author(s)
      喜多善亮
    • Organizer
      第32回日本神経科学大会
    • Place of Presentation
      名古屋
    • Related Report
      2009 Annual Research Report
  • [Presentation] ゼブラフィッシュ運動系における機能的神経回路の可視化2009

    • Author(s)
      武藤彩
    • Organizer
      第15回小型魚類研究会
    • Place of Presentation
      名古屋
    • Related Report
      2009 Annual Research Report
  • [Presentation] 触覚刺激に対する逃避行動の発達における後脳の役割2009

    • Author(s)
      浅川和秀
    • Organizer
      第15回小型魚類研究会
    • Place of Presentation
      名古屋
    • Related Report
      2009 Annual Research Report
  • [Presentation] アミノ酸への誘因行動を介在するゼブラフィッシュ嗅覚神経回路の遺伝学的解析2009

    • Author(s)
      小出哲也
    • Organizer
      第15回小型魚類研究会
    • Place of Presentation
      名古屋
    • Related Report
      2009 Annual Research Report
  • [Presentation] Tol2システムを利用した一過性レスキュー解析2009

    • Author(s)
      竹内未紀
    • Organizer
      第15回小型魚類研究会
    • Place of Presentation
      名古屋
    • Related Report
      2009 Annual Research Report
  • [Presentation] IRESを用いたTol2トランスポゾンによる遺伝子トラップ法の開発2009

    • Author(s)
      阿部玄武
    • Organizer
      第15回小型魚類研究会
    • Place of Presentation
      名古屋
    • Related Report
      2009 Annual Research Report
  • [Presentation] ゼブラフィッシュ脊髄でのpax8遺伝子の発現パターンと回路形成における役割2009

    • Author(s)
      池永隆徳
    • Organizer
      第15回小型魚類研究会
    • Place of Presentation
      名古屋
    • Related Report
      2009 Annual Research Report
  • [Presentation] Botulinum Neurotoxin transgenic zebrafish for dissecting neuronal circuits in vivo and creating novel variants for neurotherapeutics2009

    • Author(s)
      Suster, M.L.
    • Organizer
      46th Congress of the European Societies of Toxicology
    • Place of Presentation
      Dresden, Germany
    • Related Report
      2009 Annual Research Report
  • [Presentation] Apical fold morphogenesis in zebrafish fin ; differences from tetrapod limb development2009

    • Author(s)
      矢野十織
    • Organizer
      16th International Society of Developmental Biologists Congress
    • Place of Presentation
      Edinburgh, Scotland
    • Related Report
      2009 Annual Research Report
  • [Presentation] Functional analysis of the habenula in control of fear2009

    • Author(s)
      Okamoto, H.
    • Organizer
      The 4th Asia-Oceania Zebrafish Meeting
    • Place of Presentation
      Jeju, Korea
    • Related Report
      2009 Annual Research Report
  • [Presentation] Transposon-mediated gene trapping, enhancer trapping and insertional mutagenesis2009

    • Author(s)
      川上浩一
    • Organizer
      The 4th Asia-Oceania Zebrafish Meeting
    • Place of Presentation
      Jeju, Korea
    • Related Report
      2009 Annual Research Report
  • [Presentation] 生命の不思議を解き明かす光るゼブラフィッシュ2009

    • Author(s)
      川上浩一
    • Organizer
      ゲノムひろば2009 in アキバ・ゲノム研究勢ぞろい
    • Place of Presentation
      東京
    • Related Report
      2009 Annual Research Report
  • [Presentation] Functional analysis of the habenula in control of fear2009

    • Author(s)
      揚妻正和
    • Organizer
      6th European Zebrafish Genetics and Development Meeting
    • Place of Presentation
      Rome
    • Related Report
      2009 Annual Research Report
  • [Presentation] Transgenic zebrafish Tg(Nogo-B:GFP)line recapitulates the Nogo-B expression pattern in diverse tissues including the liver and intestine2009

    • Author(s)
      Han, HW
    • Organizer
      6th European Zebrafish Genetics and Development Meeting
    • Place of Presentation
      Rome
    • Related Report
      2009 Annual Research Report
  • [Presentation] Hypocretin interacts with melatonin in regulating sleep in zebrafish2009

    • Author(s)
      Appelbaum, L.
    • Organizer
      6th European Zebrafish Genetics and Development Meeting
    • Place of Presentation
      Rome
    • Related Report
      2009 Annual Research Report
  • [Presentation] A novel zebrafish cyclin dx gene : expression profile, requirement for the development of primordium motor neuron, pmn and characterization of its promoter region2009

    • Author(s)
      Cheng, C.H.
    • Organizer
      6th European Zebrafish Genetics and Development Meeting
    • Place of Presentation
      Rome
    • Related Report
      2009 Annual Research Report
  • [Presentation] Formation of spinal network dependent on domain-specific Pax genes2009

    • Author(s)
      Ono, F.
    • Organizer
      6th European Zebrafish Genetics and Development Meeting
    • Place of Presentation
      Rome
    • Related Report
      2009 Annual Research Report
  • [Presentation] The gene trap approach reveals involvement of mekk3b in the functional blood vessel formation2009

    • Author(s)
      浦崎明宏
    • Organizer
      6th European Zebrafish Genetics and Development Meeting
    • Place of Presentation
      Rome
    • Related Report
      2009 Annual Research Report
  • [Presentation] Expression of H-RASV12 in a zebrafish model of Costello syndrome causes cellular senescence in adult proliferating cells2009

    • Author(s)
      Santoriello, C.
    • Organizer
      6th European Zebrafish Genetics and Development Meeting
    • Place of Presentation
      Rome
    • Related Report
      2009 Annual Research Report
  • [Presentation] Tol2-mediated transgenesis in zebrafish and mice2009

    • Author(s)
      川上浩一
    • Organizer
      Conference on Genome Engineering
    • Place of Presentation
      Minneapolis, Minnesota
    • Related Report
      2009 Annual Research Report
  • [Presentation] The zebrafish cdc73 is essential for embryogenesis and expression of fgf24 in pectoral fin development2009

    • Author(s)
      Abe, G.
    • Organizer
      42nd Annual Meeting for the Japanese Society of Developmental Biologists
    • Place of Presentation
      新潟
    • Related Report
      2009 Annual Research Report
  • [Presentation] Characterization of gene trap and enhancer trap transgenic zebrafish lines that express GaL4 in specific region of brain in adult stage2009

    • Author(s)
      Lal, P.
    • Organizer
      42nd Annual Meeting for the Japanese Society of Developmental Biologists
    • Place of Presentation
      新潟
    • Related Report
      2009 Annual Research Report
  • [Presentation] Apical fold morphogenesis of pectoral fin in zebrafish2009

    • Author(s)
      矢野十織
    • Organizer
      42nd Annual Meeting for the Japanese Society of Developmental Biologists
    • Place of Presentation
      新潟
    • Related Report
      2009 Annual Research Report
  • [Presentation] An effective and convenient method to express transgene by the combination of in utero electroporation and the Tol2 transposon system ; inducible or cell-type specific expression in glia2009

    • Author(s)
      吉田綾子
    • Organizer
      42nd Annual Meeting for the Japanese Society of Developmental Biologists
    • Place of Presentation
      新潟
    • Related Report
      2009 Annual Research Report
  • [Presentation] Genetic inactivation of the asymmetric habenulo-interpeduncular projection enhances the conditioned fear response in zebrafish2009

    • Author(s)
      揚妻正和
    • Organizer
      The 2009 meeting on Synapses : from molecules to circuits & behavior, Cold Spring Harbor Laboratory
    • Place of Presentation
      New York
    • Related Report
      2009 Annual Research Report
  • [Presentation] Recent advances in the To12 transposon technology in zebrafish2009

    • Author(s)
      Kawakami, K.
    • Organizer
      3rd Strategic Conference of Zebrafish Investigators
    • Place of Presentation
      Asilomar, USA
    • Related Report
      2008 Annual Research Report
  • [Presentation] Jagged-Notch signaling regulates notochord morphogenesis in zebrafish2009

    • Author(s)
      Yamamoto, M.
    • Organizer
      3rd Strategic Conference of Zebrafish Investigators
    • Place of Presentation
      Asilomar, USA
    • Related Report
      2008 Annual Research Report
  • [Presentation] Targeted Gene Expression by Tol2 Transposon-mediated Gal4 Gene and Enhancer Trap Approaches in Zebrafish.2008

    • Author(s)
      Asakawa, K., Suster, M.L., Mizusawa, K., Nagayoshi, S., Kotani, T., Urasaki, A., Kishimoto, Y., Hibi, M., Kawakami, K.
    • Organizer
      8th International Conference on Zebrafish Development and Genetics
    • Place of Presentation
      Madison, Wisconsin
    • Related Report
      2009 Final Research Report
  • [Presentation] Transposon-mediated gene trapping and enhancer trapping in zebrafish2008

    • Author(s)
      Kawakami, K.
    • Organizer
      6th Annual International Conference on Transposition and Animal Biotechnology
    • Place of Presentation
      Berlin, Germany
    • Related Report
      2009 Final Research Report 2008 Annual Research Report
  • [Presentation] Genetic dissection of neural circuits by Tol2 transposon-mediated Gal4 gene and enhancer trapping in zebrafish.2008

    • Author(s)
      Kawakami, K.
    • Organizer
      Asia pacific zebrafish network meeting
    • Place of Presentation
      Auckland, New Zealand
    • Related Report
      2009 Final Research Report
  • [Presentation] 内耳でGFPを発現するゼブラフィッシュ系統を用いた変異系統の形態形成異常の解析2008

    • Author(s)
      Funahashi, J.
    • Organizer
      第31回日本分子生物学会年会第81回日本生化学会大会
    • Place of Presentation
      神戸
    • Related Report
      2008 Annual Research Report
  • [Presentation] 黒色素細胞におけるKirチャネルの発現はゼブラフィッシュのストライプ幅を制御する2008

    • Author(s)
      稲葉真史
    • Organizer
      第31回日本分子生物学会年会第81回日本生化学会大会
    • Place of Presentation
      神戸
    • Related Report
      2008 Annual Research Report
  • [Presentation] Exploiting the Tol2 transposon system for investigation of glial development in mouse central nervous system2008

    • Author(s)
      Yoshida, A.
    • Organizer
      第31回日本分子生物学会年会第81回日本生化学会大会
    • Place of Presentation
      神戸
    • Related Report
      2008 Annual Research Report
  • [Presentation] A Novel Conserved evx1 Enhancer Links Spinal Interneuron Morphology and Cis-regulation from Fish to Mammals2008

    • Author(s)
      Suster, M. L.
    • Organizer
      The 16th CDB Meeting Cis-sequence Regulation and its Evolution
    • Place of Presentation
      神戸
    • Related Report
      2008 Annual Research Report
  • [Presentation] The zebrafish parafibromin gene is essential for embryogenesis and expression of tbx5 target gines in pectoral fin development2008

    • Author(s)
      Abe, G.
    • Organizer
      第14回小型魚類研究会
    • Place of Presentation
      岡崎
    • Related Report
      2008 Annual Research Report
  • [Presentation] アミノ酸への誘引行動を介在するゼブラフィッシュ嗅覚神経回路の遺伝学的解析2008

    • Author(s)
      小出哲也
    • Organizer
      日本味と匂学会第42回大会
    • Place of Presentation
      富山
    • Related Report
      2008 Annual Research Report
  • [Presentation] BEHAVIORAL SCREENING OF GAL4-ENHANCER TRAP LINES WITH UAS-TETANUS TOXIN IN ADULT ZEBRAFISH2008

    • Author(s)
      Muto, A.
    • Organizer
      The 2008 meeting on Axon Guidance, Synaptogenesis & Neural Plasticity
    • Place of Presentation
      New York
    • Related Report
      2008 Annual Research Report
  • [Presentation] 脊椎動物発生過程においてプロテインキナーゼAの活性を制御する分子機構の解明2008

    • Author(s)
      小谷友也
    • Organizer
      日本動物学会第79回大会
    • Place of Presentation
      福岡市
    • Related Report
      2008 Annual Research Report
  • [Presentation] Genetic Dissection of Zebrafish Olfactory Circuitry Mediating Attractive Response to Amino Acids2008

    • Author(s)
      Koide, T.
    • Organizer
      International Symposium on Olfaction and Taste Meeting
    • Place of Presentation
      San Francisco
    • Related Report
      2008 Annual Research Report
  • [Presentation] Genetic dissection of neural circuits by Tol2 transposon-mediated Ga14 gene and enhancer trapping in zebrafish2008

    • Author(s)
      Kawakami, K.
    • Organizer
      6th FENS Forum of European Neuroscience
    • Place of Presentation
      Geneva Switzerland
    • Related Report
      2008 Annual Research Report
  • [Presentation] Targeted Gene Expression by Tol2 Transposon-mediated Gal4 Gene and Enhancer Trap Approaches in Zebrafish2008

    • Author(s)
      Asakawa, K.
    • Organizer
      8th International Conference on Zebrafish Development and Genetics
    • Place of Presentation
      Madison
    • Related Report
      2008 Annual Research Report
  • [Presentation] Overepression of Zebrafish IMP2 Induces Steatosis and ER Stress in the Liver of Transgenic Zebrafish as an Animal Model of Human Non-Alcoholic Fatty Liver Disease2008

    • Author(s)
      Gong, H.
    • Organizer
      8th International Conference on Zebrafish Development and Genetics
    • Place of Presentation
      Madison
    • Related Report
      2008 Annual Research Report
  • [Presentation] Functional redundancy of Tcf7 and Lef1, transcription factors mediating the Wnt signaling pathway, during zebrafish fin development2008

    • Author(s)
      Abe, G.
    • Organizer
      8th International Conference on Zebrafish Development and Genetics
    • Place of Presentation
      Madison
    • Related Report
      2008 Annual Research Report
  • [Presentation] Development of Pratform Technology for Sterile Control of Genetically Modified Fish2008

    • Author(s)
      Hu, S.
    • Organizer
      8th International Conference on Zebrafish Development and Genetics
    • Place of Presentation
      Madison
    • Related Report
      2008 Annual Research Report
  • [Presentation] Pax8 regulates development of dopaminergic neurons in diencephalon2008

    • Author(s)
      Ikenaga, T.
    • Organizer
      8th International Conference on Zebrafish Development and Genetics
    • Place of Presentation
      Madison
    • Related Report
      2008 Annual Research Report
  • [Presentation] Jagged-Notch Signaling is Involved in Zebrafish Notochord Development2008

    • Author(s)
      Yamamoto, M.
    • Organizer
      8th International Conference on Zebrafish Development and Genetics
    • Place of Presentation
      Madison
    • Related Report
      2008 Annual Research Report
  • [Presentation] Potassium ion contributes to pigment pattern formation2008

    • Author(s)
      Inaba, M.
    • Organizer
      8th International Conference on Zebrafish Development and Genetics
    • Place of Presentation
      Madison
    • Related Report
      2008 Annual Research Report
  • [Presentation] DNA Damage Response and Cellular Senescence in a Zebrafish Model of Costello Syndrome2008

    • Author(s)
      Santoriello, C.
    • Organizer
      8th International Conference on Zebrafish Development and Genetics
    • Place of Presentation
      Madison
    • Related Report
      2008 Annual Research Report
  • [Presentation] Functional redundancy of Tcf7 and Lef1, transcription factors mediating the Wnt signaling pathway, during zebrafish fin development2008

    • Author(s)
      Abe, G.
    • Organizer
      第41回日本発生生物学会
    • Place of Presentation
      徳島
    • Related Report
      2008 Annual Research Report
  • [Presentation] Jagged-Notch signahng is involved in zebrafish notochord development2008

    • Author(s)
      Yamamoto, M.
    • Organizer
      第41回日本発生生物学会
    • Place of Presentation
      徳島
    • Related Report
      2008 Annual Research Report
  • [Presentation] ゼブラフィッシュGal4トラップ法を用いた神経回路の可視化と機能阻害2008

    • Author(s)
      浅川和秀
    • Organizer
      第41回日本発生生物学会
    • Place of Presentation
      徳島
    • Related Report
      2008 Annual Research Report
  • [Presentation] Genetic Dissection of Neural Circuits by Tol2 Transposon-Mediated Gal4 and Enhancer Trapping in Zebrafish2008

    • Author(s)
      Asakawa, K.
    • Organizer
      The 6th annual CDB Symposium 2008, Turning Neurons into a Nervous System
    • Place of Presentation
      神戸
    • Related Report
      2007 Annual Research Report
  • [Presentation] Genetic dissection of neural circuits by To12 transposon-mediated Gal4 gene and enhancer trapping in zebrafish2008

    • Author(s)
      Asakawa, K.
    • Organizer
      The 2008 meeting on Neuronal Circuits : from Structure to Function, Cold Spring Harbor Laboratory
    • Place of Presentation
      New York
    • Related Report
      2007 Annual Research Report
  • [Presentation] Transposon-mediated gene trapping and enhancer trapping in zebrafish2007

    • Author(s)
      Kawakami, K.
    • Organizer
      Indian Society of Developmental Biologists
    • Place of Presentation
      Agra, India
    • Related Report
      2009 Final Research Report
  • [Presentation] Transposon-mediated gene and enhancer trapping in zebrafish.2007

    • Author(s)
      Kawakami, K.
    • Organizer
      The 16th Korea Genome Organization Conference
    • Place of Presentation
      Seoul, Korea.
    • Related Report
      2009 Final Research Report
  • [Presentation] Nrf2活性モニター用トランスジェニックゼブラフィッシュ系の開発2007

    • Author(s)
      辻田忠志
    • Organizer
      第30回日本分子生物学会年会・第80回日本生化学会大会
    • Place of Presentation
      横浜
    • Related Report
      2007 Annual Research Report
  • [Presentation] トランスポゾンを用いたGal4-UAS法による細胞の可視化と機能改変2007

    • Author(s)
      Kawakami, K.
    • Organizer
      第30回日本分子生物学会年会・第80回日本生化学会大会
    • Place of Presentation
      横浜
    • Related Report
      2007 Annual Research Report
  • [Presentation] 遺伝子トラップ系統を用いたゼブラフィッシュ三半規管形態形成の解析2007

    • Author(s)
      舟橋淳一
    • Organizer
      第30回日本分子生物学会年会・第80回日本生化学会大会
    • Place of Presentation
      横浜
    • Related Report
      2007 Annual Research Report
  • [Presentation] Transposon-mediated gene trapping and enhancer-trapping in zebrafish2007

    • Author(s)
      Kawakami, K.
    • Organizer
      ISDB
    • Place of Presentation
      Agra, India
    • Related Report
      2007 Annual Research Report
  • [Presentation] Transposon-mediated gene and enhancer trapping in zebrafish2007

    • Author(s)
      Kawakami, K.
    • Organizer
      The 16th Korea Genome Organization Conference
    • Place of Presentation
      Seoul
    • Related Report
      2007 Annual Research Report
  • [Presentation] 三次元タイムラプスによるゼブラフィッシュ三半規管形態形成の解析2007

    • Author(s)
      舟橋淳一
    • Organizer
      第13回小型魚類研究会
    • Place of Presentation
      東京
    • Related Report
      2007 Annual Research Report
  • [Presentation] 魚類の外部器官であるMitochondria-rich cells(MRCs)の複雑な発生分化機構2007

    • Author(s)
      江嵜正浩
    • Organizer
      第13回小型魚類研究
    • Place of Presentation
      東京
    • Related Report
      2007 Annual Research Report
  • [Presentation] ゼブラフィッシュバイオリソースII2007

    • Author(s)
      岡本仁
    • Organizer
      第13回小型魚類研究会
    • Place of Presentation
      東京
    • Related Report
      2007 Annual Research Report
  • [Presentation] Molybdenum cofactor biosynthesis essential for heparan sulfate formation and fgf signaling during vertebrate embryogenesis2007

    • Author(s)
      Kishimoto, Y.
    • Organizer
      5th European Zebrafish Genetics and Development Meeting
    • Place of Presentation
      Amsterdam
    • Related Report
      2007 Annual Research Report
  • [Presentation] Insertion of RFP using gene trap results in a knockdown of the pax8 gene2007

    • Author(s)
      Gebhart, N.
    • Organizer
      5th European Zebrafish Genetics and Development Meeting
    • Place of Presentation
      Amsterdam
    • Related Report
      2007 Annual Research Report
  • [Presentation] Visualization in zebrafish larvae of Na+ uptake in mitochondria-rich cells whose differentiation is dependent on foxi3A2007

    • Author(s)
      Esaki, M.
    • Organizer
      5th European Zebrafish Genetics and Development Meeting
    • Place of Presentation
      Amsterdam
    • Related Report
      2007 Annual Research Report
  • [Presentation] Analysis of conserved element regulating expression of fgf8 in zebrafish2007

    • Author(s)
      Komisarczuk, A.
    • Organizer
      5th European Zebrafish Genetics and Development Meeting
    • Place of Presentation
      Amsterdam
    • Related Report
      2007 Annual Research Report
  • [Presentation] Targeted gene expression by GAL4 gene and enhancer trapping in zebrafish defines subsets of neurons required for simple vetebrate behaviors2007

    • Author(s)
      Asakawa, K.
    • Organizer
      5th European Zebrafish Genetics and Development Meeting
    • Place of Presentation
      Amsterdam
    • Related Report
      2007 Annual Research Report
  • [Presentation] New genetic techniques by excision of the To12 transposon : isolation of revertants and creating frame shift mutations2007

    • Author(s)
      Urasaki, A.
    • Organizer
      5th European Zebrafish Genetics and Development Meeting
    • Place of Presentation
      Amsterdam
    • Related Report
      2007 Annual Research Report
  • [Presentation] ゼブラフィッシュにおける新しい遺伝学的方法論の確立2007

    • Author(s)
      川上浩一
    • Organizer
      東洋紡バイオ財団設立25周年記念シンポジウム「次世代へのメッセージ」
    • Place of Presentation
      大阪
    • Related Report
      2007 Annual Research Report
  • [Presentation] Somitic contribution to the formation of dorsal aorta involves cell migration regulated by Notch and ephrin2007

    • Author(s)
      Yoshiko Takahashi
    • Organizer
      第40回日本発生生物学会, 第59回日本細胞生物学会合同大会
    • Place of Presentation
      福岡
    • Related Report
      2007 Annual Research Report
  • [Presentation] ニワトリ胚視蓋極性形成におけるEnとFgfシグナルの役割2007

    • Author(s)
      原田英斉
    • Organizer
      第40回日本発生生物学会, 第59回日本細胞生物学会合同大会
    • Place of Presentation
      福岡
    • Related Report
      2007 Annual Research Report
  • [Presentation] ゼブラフィッシュGAL4トラップ法を用いた行動制御に関与する神経細胞の探索2007

    • Author(s)
      浅川和秀
    • Organizer
      第40回日本発生生物学会, 第59回日本細胞生物学会合同大会
    • Place of Presentation
      福岡
    • Related Report
      2007 Annual Research Report
  • [Presentation] ゼブラフィッシュにおけるJump starterトランスポゾンシステムの開発2007

    • Author(s)
      浦崎明宏
    • Organizer
      第40回日本発生生物学会, 第59回日本細胞生物学会合同大会
    • Place of Presentation
      福岡
    • Related Report
      2007 Annual Research Report
  • [Presentation] Xenopus胚において活性をもつ組換えTo12移転酵素の精製2007

    • Author(s)
      柴野卓志
    • Organizer
      第40回日本発生生物学会, 第59回日本細胞生物学会合同大会
    • Place of Presentation
      福岡
    • Related Report
      2007 Annual Research Report
  • [Presentation] ゼブラフィッシュ幼生におけるNaイオン吸収細胞の同定とその発生分化機構の解析2007

    • Author(s)
      江崎正浩
    • Organizer
      第40回日本発生生物学会, 第59回日本細胞生物学会合同大会
    • Place of Presentation
      福岡
    • Related Report
      2007 Annual Research Report
  • [Presentation] 遺伝子トラップ系統を用いたゼブラフィッシュ三半規管形態形成の解析2007

    • Author(s)
      舟橋淳一
    • Organizer
      第40回日本発生生物学会, 第59回日本細胞生物学会合同大会
    • Place of Presentation
      福岡
    • Related Report
      2007 Annual Research Report
  • [Presentation] モリブデン補酵素生合成は、硫酸イオン生成を通じて脊椎動物胚発生におけるFgfシグナル伝達に必須の役割を果たす2007

    • Author(s)
      岸本康之
    • Organizer
      第40回日本発生生物学会, 第59回日本細胞生物学会合同大会
    • Place of Presentation
      福岡
    • Related Report
      2007 Annual Research Report
  • [Presentation] Development of the Gal4-enhancer trap system using the Tol2 transposable element and its application to inhibition of synaptic transmission in zebrafish.2006

    • Author(s)
      Asakawa, K., Mizusawa, K., Urasaki, A., Kotani, T., Nagayoshi, S., Kishimoto, Y., Hibi, M., Kawakami, K.
    • Organizer
      7th International Conference on Zebrafish Development & Genetics
    • Place of Presentation
      Madison, Wisconsin.
    • Related Report
      2009 Final Research Report
  • [Presentation] Remobilization of integrated transposons: the jump starter system in zebrafish2006

    • Author(s)
      Urasaki, A., Asakawa, K., Kawakami, K.
    • Organizer
      7th International Conference on Zebrafish Development & Genetics
    • Place of Presentation
      Madison, Wisconsin
    • Related Report
      2009 Final Research Report
  • [Presentation] The Tol2 transposable element: minimum cis-requirements and remobilization2006

    • Author(s)
      Kawakami, K.
    • Organizer
      ASM conferences: Mobile DNA
    • Place of Presentation
      Banff, Canada
    • Related Report
      2009 Final Research Report
  • [Presentation] Gene and enhancer trapping using the Tol2 transposon system.2005

    • Author(s)
      Kawakami, K.
    • Organizer
      Strategic Conference of Zebrafish Investigators
    • Place of Presentation
      Salisbury Cove, Maine
    • Related Report
      2009 Final Research Report
  • [Presentation] Development of the Gal4-enhancer trap system using the Tol2 transposable element in zebrafish2005

    • Author(s)
      Asakawa, K., Ito, A., Urasaki, A., Kotani, T., Nagayoshi, S., Kishimoto, Y., Hibi, M., Kawakami, K.
    • Organizer
      2005 West Coast Zebrafish Meeting
    • Place of Presentation
      Eugene, Oregon
    • Related Report
      2009 Final Research Report
  • [Presentation] The Tol2-mediated genetic methodologies in zebrafish: gene trapping, enhancer trapping and remobilization.2005

    • Author(s)
      Urasaki, A., Asakawa, K., Kotani, T., Nagayoshi, S., Kishimoto, Y., Kawakami, K.
    • Organizer
      4th European Zebrafish Genetics and Development Meeting
    • Place of Presentation
      Dresden, Germany.
    • Related Report
      2009 Final Research Report
  • [Presentation] The Tol2 transposable element: efficient gene and enhancer trapping in vertebrate2005

    • Author(s)
      Kawakami, K.
    • Organizer
      FASEB Summer Research Conferences "Mammalian Mobile Elements"
    • Place of Presentation
      Tucson, Arizona.
    • Related Report
      2009 Final Research Report
  • [Book] Essential zebrafish methods: genetics and genomics(The transgensis and gene and enhancer trap methods in zebrafish by using the Tol2 transposable element)2009

    • Author(s)
      Kawakami, K.
    • Publisher
      Academic Press
    • Related Report
      2009 Final Research Report
  • [Book] Methods in Molecular Biology(Zebrafish: methods and protocols)2009

    • Author(s)
      Lieschke, G.J., Oates, A.C., Kawakami, K.
    • Total Pages
      335
    • Publisher
      Humana Press
    • Related Report
      2009 Final Research Report
  • [Book] Zebrafish : methods and protocols2009

    • Author(s)
      Lieschke, G.J.
    • Total Pages
      335
    • Publisher
      Humana Press, New York
    • Related Report
      2009 Annual Research Report
  • [Remarks]

    • URL

      http://kawakami.lab.nig.ac.jp/

    • Related Report
      2009 Final Research Report
  • [Remarks] zTrap: zebrafish gene trap and enhancer trap database

    • URL

      http://kawakami.lab.nig.ac.jp/zTrap/

    • Related Report
      2009 Final Research Report
  • [Remarks] 毎日新聞2008年1月15日掲載「魚の神経回路を制御」

    • Related Report
      2009 Final Research Report
  • [Remarks]

    • URL

      http://kawakami.lab.nig.ac.jp/

    • Related Report
      2009 Annual Research Report
  • [Remarks]

    • URL

      http://kawakami.lab.nig.ac.jp/

    • Related Report
      2008 Annual Research Report
  • [Remarks]

    • URL

      http://kawakami.lab.nig.ac.jp/

    • Related Report
      2007 Annual Research Report
  • [Patent(Industrial Property Rights)] ボツリヌス毒素遺伝子トランスジェニックフィッシュ2009

    • Inventor(s)
      川上浩一, サスター, マキシミリアーノ
    • Industrial Property Rights Holder
      大学共同利用機関法人情報・システム研究機構
    • Industrial Property Number
      2009-211821
    • Filing Date
      2009-09-14
    • Related Report
      2009 Annual Research Report 2009 Final Research Report

URL: 

Published: 2005-04-01   Modified: 2018-03-28  

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