Comprehensive analysis of interactions between plants and microbes

• Project/Area Number: 17018041
• Research Category: Grant-in-Aid for Scientific Research on Priority Areas
• Allocation Type: Single-year Grants
• Review Section: Biological Sciences
• Research Institution: Kazusa DNA Research Institute
• Principal Investigator: TABATA Satoshi Kazusa DNA Research Institute, 副所長 (70197549)
• Co-Investigator(Kenkyū-buntansha): KAWATUCHI Masayoshi 基礎生物学研究所, 共生システム研究部門, 教授 (30260508) ; SAEKI Kazuhiko 奈良女子大学, 理学部, 教授 (40201511) ; MINAMISAWA Kiwamu 東北大学, 大学院・生命科学研究科, 教授 (70167667) ; 中村 保一 財団法人かずさDNA研究所, 植物ゲノム研究部・植物ゲノム情報研究室, 室長 (60370920)
• Project Period (FY): 2005 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥146,900,000 (Direct Cost: ¥146,900,000); Fiscal Year 2009: ¥29,000,000 (Direct Cost: ¥29,000,000); Fiscal Year 2008: ¥29,000,000 (Direct Cost: ¥29,000,000); Fiscal Year 2007: ¥28,700,000 (Direct Cost: ¥28,700,000); Fiscal Year 2006: ¥28,700,000 (Direct Cost: ¥28,700,000); Fiscal Year 2005: ¥31,500,000 (Direct Cost: ¥31,500,000)
• Keywords: マメ科植物 / ミヤコグサ / 根粒菌 / 共生窒素固定 / タンパク質相互作用 / 共生 / 窒素固定

Research Abstract

Molecular mechanisms of symbiotic nitrogen-fixation between legumes and rhizobia have been investigated by molecular-genetics and genomics approaches. Genes that are essential or related to the establishment of symbiosis, nodule formation and its regulation, and nitrogen fixation were isolated, and their functions were identified.

Research Products

• [Journal Article] Apoplastic plant subtilases support arbuscular mycorrhiza development in Lotus janonicus (2009)
  • Author(s): Takeda, N., 他
  • Journal Title: Plant J. 58 Pages: 766-777
  • Peer Reviewed
• [Journal Article] The temperature sensitive brush mutant of the legume Lotus japonicus reveals a link between root development and nodule infection by rhizobia (2009)
  • Author(s): Maekawa-Yoshikawa, M., 他
  • Journal Title: Plant Physiol. 149 Pages: 1785-1796
  • Peer Reviewed
• [Journal Article] Rearrangement of Actin Cytoskeleton Mediates Invasion of Lotus japonicus Roots by Mesorhizobium loti (2009)
  • Author(s): Yokota, K, 他
  • Journal Title: Plant Cell 21 Pages: 267-284
  • Peer Reviewed
• [Journal Article] A positive regulatory role for LjERF1 in the nodulation process is revealed by systematic analysis of nodule-associated transcription factors of Lotus japonicus. (2008)
  • Author(s): Asamizu, E., Shimoda, Y., Kouchi, H., Tabata, S., Sato, S.
  • Journal Title: Plant Physiol. 147(4) Pages: 2030-2040
• [Journal Article] A large scale analysis of protein-protein interactions in the nitrogenfixing bacterium Mesorhizobium loti (2008)
  • Author(s): Shimoda, Y., Shinpo, S., Kohara, M., Nakamura, Y., Tabata, S., Sato, S.
  • Journal Title: DNA Res. 15(1) Pages: 13-23
• [Journal Article] A positive regulatory role for LjERF1 in the nodulation process is revealed by systematic analysis of nodule-associated transcription factors of Lotus japonicus. (2008)
  • Author(s): Asamizu, E.
  • Journal Title: Plant Physiology 147 Pages: 2030-2040
  • Peer Reviewed
• [Journal Article] Construction of signature-tagged mutant library in Mesorhizobium loti as a powerful tool for functional genomics. (2008)
  • Author(s): Shimoda, Y.
  • Journal Title: DNA Research 15 Pages: 297-308
  • Peer Reviewed
• [Journal Article] A large scale analysis of protein-protein interactions in the nitrogen fixing bacterium Mesorhizobium loti (2008)
  • Author(s): Shimoda, Y.
  • Journal Title: DNA Research 15 Pages: 13-23
  • Peer Reviewed
• [Journal Article] CYCLOPS, a mediator of symbiotic intracellular accommodation. (2008)
  • Author(s): Yano, K.
  • Journal Title: Proc. Natl. Acad. Sci. USA 105 Pages: 20540-20545
  • Peer Reviewed
• [Journal Article] Polyubiquitin Promoter-Based Binary Vectors for Overexpression and Gene Silencing in Lotus japonicus (2008)
  • Author(s): Maekawa, T.
  • Journal Title: Molecular Plant Microbe Interaction 21 Pages: 375-382
  • Peer Reviewed
• [Journal Article] Requirement of Mesorhizobium loti Ornithine Transcarbamoylase for Successful Symbiosis with Lotus japonicus as Revealed by an Unexpected Long-Range Genome Deletion (2008)
  • Author(s): Mishima, E.
  • Journal Title: Plant Cell Physiol. 49 Pages: 301-313
  • Peer Reviewed
• [Journal Article] NUCLEOPORIN85 is required for calcium spiking, fungal and bacterial symbioses and seed production in Lotus japonicus. (2007)
  • Author(s): Saito K, Yoshikawa M, Yano K, Miwa H, Uchida H, Asamizu E, Sato S, Tabata S, Imaizumi-Anraku H, Umehara Y, Kouchi H, Murooka Y, Szczyglowski K, Downie JA, Parniske M, Hayashi M, Kawaguchi M.
  • Journal Title: Plant Cell 19 Pages: 610-624
• [Journal Article] NUCLEOPORIN85 Is Required for Calcium Spiking, Fungal and Bacterial Symbioses, and Seed Production in Lotus japonicus (2007)
  • Author(s): Saito, K.
  • Journal Title: Plant Cell 19 Pages: 610-624
  • Peer Reviewed
• [Journal Article] Genome sequencing and genome resources in model legumes (2007)
  • Author(s): Sato, S.
  • Journal Title: Plant Physiology 144 Pages: 588-593
  • Peer Reviewed
• [Journal Article] The Mesorhizobium loti purB gene is involved in infection thread formation and nodule development in Lotus japonicus (2007)
  • Author(s): Okazaki, S.
  • Journal Title: J. of Bacteriology 189 Pages: 8347-8352
  • Peer Reviewed
• [Journal Article] A novel ankyrin-repeat membrane protein, IGN1, is required for persistence of nitrogen-fixing symbiosis in root nodules of Lotus japonicus. (2007)
  • Author(s): Kumagai, H.
  • Journal Title: Plant Physiol. 143 Pages: 1293-1305
  • Peer Reviewed
• [Journal Article] Characterization of genomic clones and expression analysis of the three types of superoxide dismutases during nodule development in Lotus japonicus. (2007)
  • Author(s): Rubio, M. C.
  • Journal Title: Mol Plant Microbe Interact. 20 Pages: 262-275
  • Peer Reviewed
• [Journal Article] A Cytokinin Perception Mutant Colonized by Rhizobium in the Absence of Nodule Organogenesis (2007)
  • Author(s): Murray, J. D.
  • Journal Title: Science 315 Pages: 101-104
  • Peer Reviewed
• [Journal Article] Legume genome evolution viewed through the Medicago truncatula and Lotus japonicus genomes (2006)
  • Author(s): Cannon, S.B., Sterck, L., Rombauts, S., Sato, S., Cheung, F., Gouzy, J., Wang, X., Mudge, J., Vasdewani, J., Schiex, T.et al.
  • Journal Title: Proc. Natl. Acad. Sci. U.S.A 103 Pages: 14959-14964
• [Journal Article] Deregulation of a Ca2+/calmodulin-dependent kinase leads to spontaneous nodule development (2006)
  • Author(s): Tirichine, L., Imaizumi-Anraku, H., Yoshida, S., Murakami, Y., Madsen, L.H., Miwa, H., Nakagawa, T., Sandal, N., Albrektsen, A.S., Kawaguchi, M.et al.
  • Journal Title: Nature 441 Pages: 1153-1156
• [Journal Article] Biosynthesis of ascorbic acid in legume root nodules (2006)
  • Author(s): Matamoros, M.A., Loscos, J., Coronado, M.J., Ramos, J., Sato, S., Testillano, P.S., Tabata, S., Becana, M.
  • Journal Title: Plant Physiology 141 Pages: 1068-1077
• [Journal Article] Control of petal shape and floral zygomorphy in Lotus japonicus (2006)
  • Author(s): Feng, X., Zhao, Z, Tian, Z., Xu, S., Luo, Y., Cai, Z., Wang, Y., Fang, J., Wang, Z., Weng, L.et al.
  • Journal Title: Proc. Natl. Acad. Sci. U.S.A. 103 Pages: 4970-4975
• [Journal Article] Lotus japonicus as a platform for legume research (2006)
  • Author(s): Sato, S., Tabata, S.
  • Journal Title: Curr. Opin. Plant Biol. 9 Pages: 128-132
• [Journal Article] A nucleoporin is required for induction of Ca2+ spiking in legume nodule development and essential for rhizobial and fungal symbiosis (2006)
  • Author(s): Kanamori, N., Madsen, L.H., Radutoiu, S., Frantescu M., Quistgaard, E.M., Miwa, H., Downie, J.A., James, E.K., Felle, H.H., Haaning, L.L.et al.
  • Journal Title: Proc. Natl. Acad. Sci U.S.A. 103 Pages: 359-364
• [Journal Article] Genetics of Symbiosis in Lotus japonicus : Recombinant Inbred Lines, Comparative genetic maps and map position of 35 symbiotic loci (2006)
  • Author(s): Sandal N., Petersen, T.R., Murray, J., Umehara, Y., Karas, B., Yano, K., Kumagai, H., Yoshikawa, M., Saito, K., Hayashi, M.et al.
  • Journal Title: Molecular Plant-Microbe Interactions 19 Pages: 80-91
• [Journal Article] Plastid proteins crucial for symbiotic fungal and bacterial entry into plant roots (2005)
  • Author(s): Imaizumi-AnraKu, H., Takeda, W., Charpentier, M., Perry, J., Miwa, H., Umehara, Y., Kouchi, H., Murakami, Y., Mulder, L., Vickers, K., et al.
  • Journal Title: Nature 433 Pages: 527-531
• [Journal Article] Lotus burttii takes a position of the third corner in the Lotus molecular genetics triangle (2005)
  • Author(s): Kawagucni, M., Pedrosa-Harand, A., Yano, K., Hayashi, M., Murooka, Y., Saito, K., Nagata, T., Namai, K., Nishida, H., Shibata, D., et al.
  • Journal Title: DNA Research 12 Pages: 63-71
• [Journal Article] Characterization of a Lotus japonicus symbiotic mutant, lot1, that shows a reduced nodule number and distorted trichomes (2005)
  • Author(s): Ooki, Y., Banba, M., Yano, K., Maruya, J., Sato, S., Tabata, S., Saeki, K., Hayashi, M., Kawaguchi, M., Izui, K.et al.
  • Journal Title: Plant Physiology 137 Pages: 1261-1271
• [Journal Article] The sulfate transporter SST1 is crucial for symbiotic nitrogen fixation in Lotusjaponicus root nodules (2005)
  • Author(s): Krusell, L., Krause, K., Ott, T., Desbrosses, G., Kraemer, U., Sato, S., Nakamura, Y., Tabata, S., James, E.K., Sandal, N et al.
  • Journal Title: Plant Cell 17 Pages: 1625-1636
• [Journal Article] klavier (klv), a novel hypernodulation mutant of Lotus japonicus affectedin leaf vein and floral induction (2005)
  • Author(s): Oka-Kira, E., Tateno, K., Miura, K., Haga, T., Hayashi, M., Harada, K., Sato, S., Tabata, S., Shikazono, N., Tanaka, A.et al.
  • Journal Title: Plant Journal 44 Pages: 505-515
• [Presentation] ミヤコグサにおけるCLV3様遺伝子の機能解析 (2010)
  • Author(s): 岡本暁, 他
  • Organizer: 第51回日本植物生理学会年会
  • Place of Presentation: 熊本大学
  • Year and Date: 2010-03-18
• [Presentation] 根粒形成をシステミックに抑制する2つの誘導性CLE遺伝子 (2009)
  • Author(s): 岡本曉
  • Organizer: 第50回日本植物生理学会年会
  • Place of Presentation: 名古屋大学
  • Year and Date: 2009-03-23
• [Presentation] ゲノミクス技法・情報に基づく根粒菌側共生遺伝子の同定 (2009)
  • Author(s): 佐伯和彦
  • Organizer: 第50回日本植物生理学会年会
  • Place of Presentation: 名古屋大学
  • Year and Date: 2009-03-23
• [Presentation] 窒素源による根粒形成抑制機構に関わるミヤコグサペプチドの探索 (2008)
  • Author(s): 大西恵理香
  • Organizer: 第49回日本植物生理学会年会
  • Place of Presentation: 札幌コンベンションセンター
  • Year and Date: 2008-03-20
• [Presentation] ミヤコグサにおけるCLV3様遺伝子の機能解析
  • Author(s): 岡本暁、中川知己、佐藤修正、田畑哲之、川口正代司
  • Organizer: 第51回日本植物生理学会年会
• [Presentation] ミヤコグサ根粒菌STM変異株の共生に関する表現型とタンパク質間相互作用情報を基にした新規共生関連遺伝子の機能類推
  • Author(s): 中務弘基、下田宣司、中村保一、田畑哲之、佐藤修正
  • Organizer: 第32回日本分子生物学会年会
• [Remarks] 根粒菌タンパク質間相互作用DB
  • URL: http://genome.kazusa.or.jp/rhizobase/
• [Remarks] 根粒菌タンパク質問相互作用DB
  • URL: http://genome.kazusa.or.jp/rhizobase/