Analysis in pathogenesis of bacterial lethal soft-tissue infection and application to prevention and therapy.

• Project/Area Number: 17019021
• Research Category: Grant-in-Aid for Scientific Research on Priority Areas
• Allocation Type: Single-year Grants
• Review Section: Biological Sciences
• Research Institution: Kanazawa University
• Principal Investigator: SHIMIZU Tohru Kanazawa University, 医学系, 教授 (80235655)
• Co-Investigator(Kenkyū-buntansha): OHTANI Kaori 金沢大学, 医学系, 講師 (30377410) ; NAKAGAWA Ichiro 東京医科歯科大学, 医歯薬総合研究科, 教授 (70294113)
• Project Period (FY): 2005 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥104,000,000 (Direct Cost: ¥104,000,000); Fiscal Year 2009: ¥20,500,000 (Direct Cost: ¥20,500,000); Fiscal Year 2008: ¥20,500,000 (Direct Cost: ¥20,500,000); Fiscal Year 2007: ¥20,900,000 (Direct Cost: ¥20,900,000); Fiscal Year 2006: ¥21,100,000 (Direct Cost: ¥21,100,000); Fiscal Year 2005: ¥21,000,000 (Direct Cost: ¥21,000,000)
• Keywords: 細菌感染症 / ゲノム解析 / トランスクリプトーム / 遺伝子発現制御 / 病原性 / ウェルシュ菌 / A群レンサ球菌 / マイクロアレイ / 遺伝子発現調節 / オートファジー / 遺伝子発現解析 / 二成分制御系 / オートインデューサー / 細胞壁 / 細胞内寄生性細菌 / DNAマイクロアレイ / エンドサイトーシス / ストレプトリジンO

Research Abstract

In Clostridium perfringens and Streptococcus pyogenes, both of which causes severe soft-tissue infection (flesh-eating bacteria), we proceed comprehensive analyses by genome sequencing and DNA microarray experiments. We found that these bacteria share common mechanism using small non-coding RNA for the control of virulence expression and that both have different unique mechanism for genome maintenance and total regulatory system for virulence control. These research would be important for finding molecular targets for prevention and/or therapy, which will lead to the innovation of new bacterial infection control.

Research Products

• [Journal Article] Identification of a two-component VirR/VirS regulon in Clostridium perfringens (2010)
  • Author(s): Ohtani, K., Hirakawa, H., Tashiro, K., Yoshizawa, S., Kuhara, S., Shimizu, T.
  • Journal Title: Anaerobe (in press)
  • Peer Reviewed
• [Journal Article] Genetic and biochemical analysis of a class C nonspecific acid phosphatase (NSAP) of Clostridium perfringens (2010)
  • Author(s): Wang, R., Ohtani, K., Wang, Y., Yuan, Y., Hassan, S., Shimizu, T.
  • Journal Title: Microbiol 156 Pages: 167-173
  • Peer Reviewed
• [Journal Article] Identification of a two-component VirR/VirS regulon in Clostridium perfringens (2010)
  • Author(s): Ohtani, K., et al.
  • Journal Title: Anaerobe (in press)
  • Peer Reviewed
• [Journal Article] Genetic and biochemical analysis of a class C nonspecific acid phosphatase (NSAP) of Clostridium perfringens (2010)
  • Author(s): Wang, R., et al.
  • Journal Title: Microbiology 156 Pages: 167-173
  • Peer Reviewed
• [Journal Article] Contact with enterocyte-like Caco-2 cells induces rapid upregulation of toxin production by Clostridium perfringens type C isolates (2009)
  • Author(s): Vidal, J.E., Ohtani, K., Shimizu, T., McClane, B.A.
  • Journal Title: Cell. Microbiol. 11 Pages: 1306-1328
  • Peer Reviewed
• [Journal Article] Virulence gene regulation by the agr system in Clostridium perfringens (2009)
  • Author(s): Ohtani, K., Yuan, Y., Hassan, S., Wang, R., Wang, Y., Shimizu, T.
  • Journal Title: J. Bacteriol. 191 Pages: 3919-3927
  • Peer Reviewed
• [Journal Article] Comparative genomic analyses of Streptococcus mutans provide insights into chromosomal shuffling and species-specific content (2009)
  • Author(s): Maruyama, F., Kobata, M., Kurokawa, K., Nishida, K., Sakurai, A., Nakano, K., Nomura, R., Kawabata, S., Ooshima, T., Nakai, K., Hattori, M., Hamada, S., Nakagawa, I.
  • Journal Title: BMC Genomics 10 Pages: 358-358
  • Peer Reviewed
• [Journal Article] Cost-effective DNA sequencing and template preparation from bacterial colony and plasmid (2009)
  • Author(s): Maruyama, F., Nozawa, T., Aikawa, C., Sakurai, A., Nakagawa, I.
  • Journal Title: J. Biosci. Bioeng. 107 Pages: 471-473
  • NAID: 110007225791
  • Peer Reviewed
• [Journal Article] Virulence gene regulation by the agr system in Clostridium perfringens (2009)
  • Author(s): Ohtani, K, et al.
  • Journal Title: Journal of Bacteriology 191 Pages: 3919-3927
  • Peer Reviewed
• [Journal Article] Virulence gene regulation by the agr system in Clostridium perfringens (2009)
  • Author(s): K. Ohtani, et.al.
  • Journal Title: J. Bacteriol (印刷中)
  • Peer Reviewed
• [Journal Article] Cost-effective DNA sequencing and template preparation from bacterial colony and plasmid (2009)
  • Author(s): F. Maruyama, et.al.
  • Journal Title: J. Biosci. Bioeng (印刷中)
  • Peer Reviewed
• [Journal Article] Streptococcus pyogenes degrades extracellular matrix in chondrocytes via MMP-13 (2009)
  • Author(s): A. Sakurai, et.al.
  • Journal Title: Biochem Biophys Res Commun (印刷中)
  • Peer Reviewed
• [Journal Article] Streptococcus pyogenes degrades extracellular matrix in chondrocytes via MMP-13, Biochem. (2008)
  • Author(s): Sakurai, A., Okahashi, N., Maruyama, F., Ooshima, T., Hamada, S., Nakagawa, I.
  • Journal Title: Biophys. Res. Commun. 373 Pages: 450-454
  • Peer Reviewed
• [Journal Article] Demonstration of mother to child transmission of Streptococcus mutans using multilocus sequence typing (2008)
  • Author(s): Lapirattanakul, J., Nakano, K., Nomura R., Hamada, S., Nakagawa, I., Ooshima, T
  • Journal Title: Caries Res. 42 Pages: 466-474
  • Peer Reviewed
• [Journal Article] Characterization of Genes Regulated Directly by the VirR/VirS System in Clostridium perfringens (2008)
  • Author(s): Okumura, K., Ohtani, K., Hayashi, H., Shimizu, T.
  • Journal Title: J. Bacteriol. 190(23) Pages: 7719-7727
  • Peer Reviewed
• [Journal Article] Carbon catabolite repression of type IV pilus-dependent gliding motility in the anaerobic pathogen Clostridium perfringens (2008)
  • Author(s): Mendez, M., Huang, I.H., Ohtani, K., Grau, R., Shimizu, T., Sarker, M.R.
  • Journal Title: J. Bacteriol. 190 Pages: 48-60
  • Peer Reviewed
• [Journal Article] Characterization of genes regulated directlY by the VirR/VirS system in Clostridium perfringens (2008)
  • Author(s): K. Okumura, et.al.
  • Journal Title: J. Bacteriol 190 Pages: 7719-7727
  • Peer Reviewed
• [Journal Article] Carbon catabolite repression of type IV pilus-dependent gliding motility in the anaerobic pathogen Clostridium perfringens (2008)
  • Author(s): Mendez M., Huang IH., Ohtani K., Grau R., Shimizu T. and Sarker, MR.
  • Journal Title: J. Bacteriol. 190 Pages: 48-60
  • Peer Reviewed
• [Journal Article] Streptococcus mutans Exhibits Clonal Variation as Revealed by Multilocus Sequence Typing (2007)
  • Author(s): Nakano, K., Nakano, K,, Lapirattanakul, J., Nomura, R., Nemoto, H., Alaluusua, S., Gronroos, L., Vaara, M., Hamada, S., Ooshima, T., Nakagawa, I.
  • Journal Title: J. Clin. Microbiol. 45 Pages: 2616-2625
  • Peer Reviewed
• [Journal Article] Involvement of Hrs in Destruction of Group A Streptococcus (GAS):Regulation of Autophagosome Maturation (2007)
  • Author(s): Tamai, K., Tanaka, K., Nara, A., Yamamoto, A., Nakagawa, I., Yoshimori, T., Ueno, Y., Shimosegawa, T., Sugamura K.
  • Journal Title: Biochem. Biophys. Res. Commun. 360 Pages: 721-727
  • Peer Reviewed
• [Journal Article] Virulence of Porphyromonas gingivalis is altered by substitution of fimbria gene with different genotype (2007)
  • Author(s): Kato T, Kawai S, Nakano K, Inaba H, Kuboniwa M, Nakagawa I, Tsuda K, Omori H, Ooshima T, Yoshimori T, Amano A.
  • Journal Title: Cell. Microbiol. 9 Pages: 753-765
  • Peer Reviewed
• [Journal Article] Streptocccus mutans exhibits clonal variations as revealed by multilocus sequence typing (2007)
  • Author(s): Nakano N, Lapirattanakul J, Nomura R, Nemoto H, Alaluusua S, Groroos L., Vaara M, Hamada S, Ooshima T, Nakagawa I.
  • Journal Title: J. Clin. Microbiol., J. Clin. Microbiol. 45 Pages: 2616-2625
  • Peer Reviewed
• [Journal Article] Detection of a group II intron without open reading frame in the alpha toxin gene of Clostridium perfringens isolated from a broiler chicken (2007)
  • Author(s): Ma, M., Ohtani, K., Shimizu, T., and Misawa, N.
  • Journal Title: J. Bacteriol. 189 Pages: 1633-1640
  • Peer Reviewed
• [Journal Article] Streptococcus mutans Exhibits Clonal Variation as Revealed by Multilocus Sequence Typing (2007)
  • Author(s): Nakano, K., Nakano K, Lapirattanakul J, Nomura R, Nemoto H, Alaluusua S, Gronroos L, Vaara M, Hamada S, Ooshima T and Nakagawa I.
  • Journal Title: J. Clin. Microbiol. 45 Pages: 2616-2625
  • Peer Reviewed
• [Journal Article] Detection of a group II intron without open reading frame in the alpha toxin gene of Clostridium perfringens isolated from a broiler chicken (2007)
  • Author(s): Ma, M., Ohtani, K., Shimizu, T., Misawa, N.
  • Journal Title: J.Bacteriol. 60 Pages: 1633-1640
• [Journal Article] Streptocccus mutans exhibits clonal variations as revealed by multilocus sequence typing (2007)
  • Author(s): Nakano N, Lapirattanakul J, Nomura R, Nemoto H, Alaluusua S, Groroos L., Vaara M, Hamada S, Ooshima T, Nakagawa I
  • Journal Title: J.Clin.icrobiol (印刷中)
• [Journal Article] Virulence of Porphyromonas gingivalis is altered by substitution of fimbria gene with different genotype (2007)
  • Author(s): Kato T, Kawai S, Nakano K, Inaba H, Kuboniwa M, Nakagawa I, Tsuda K, Omori H, Ooshima T, Yoshimori T, Amano A
  • Journal Title: Cell.Microbiol (印刷中)
• [Journal Article] Autophagy in innate immunity against intracellular bacteria. (2006)
  • Author(s): Amano A, Nakagawa I, Yoshimori T.
  • Journal Title: J. Biochem. (Tokyo) 140 Pages: 161-166
  • NAID: 10018842714
  • Peer Reviewed
• [Journal Article] Autophagy in innate immunity against intracellular bacteria. (2006)
  • Author(s): Amano A, Nakagawa I, Yoshimori T
  • Journal Title: J.Biochem.(Tokyo) 140 Pages: 161-166
  • NAID: 10018842714
• [Journal Article] Invasion of epithelial cells and proteolysis of cellular focal adhesion components by distinct types of Porphyromonas gingivalis fimbriae (2006)
  • Author(s): NaKagawa I, Inaba H, Yamamura T, Kato T, Kawai S, Ooshima T, Amano A
  • Journal Title: Infect.Immun. 74 Pages: 3773-3782
• [Journal Article] Role of glucose side chains with serotype-specific polysaccharide in the cariogenicity of Streptococcus mutans (2005)
  • Author(s): Nakano, K., Nomura, R., Nakagawa, I., Hamada, S., Ooshima, T.
  • Journal Title: Caries Res. 39 Pages: 262-268
  • Peer Reviewed
• [Journal Article] Identification and characterization of a cell-wall anchored DNase gene in Clostridium perfringens (2005)
  • Author(s): Okumura, K., Kawsar, H.I., Shimizu, T., Ohta T.,, Hayashi H., Shimizu, T.
  • Journal Title: FEMS Microbiol. Lett. 242 Pages: 281-285
  • Peer Reviewed
• [Journal Article] Identification and characterization of a cell-wall anchored DNase gene in Clostridium perfringens (2005)
  • Author(s): Okumura, K
  • Journal Title: FEMS Microbiol.Lett. 242 Pages: 281-285
• [Journal Article] Role of glucose side chains with serotype-specific polysaccharide in the cariogenicity of Streptococcus mutans (2005)
  • Author(s): Nakano, K.
  • Journal Title: Caries Res. 39 Pages: 262-268
• [Journal Article] Distribution of Porphyromonas gingivalis fimA genotypes in Japanese children and adolescents (2005)
  • Author(s): Tamura, K.
  • Journal Title: J.Periodontol. 76 Pages: 674-679
• [Journal Article] Inhibitory effects of Pofphyromonas gingivalis fimbriae on interactions between extracellular matrix proteins and cellular integrins (2005)
  • Author(s): Nakagawa, I.
  • Journal Title: Microbes Infect. 7 Pages: 157-163
• [Presentation] Complex regulatory networks for virulence in Clostridium perfringens (2009)
  • Author(s): Shimizu, T
  • Organizer: The 6th Clostridia: The Impact of Genomics on Disease Control
  • Place of Presentation: Instito Superiore di Sanita
  • Year and Date: 2009-10-21
• [Presentation] Complex regulatory networks for virulence in C.perfringens (2009)
  • Author(s): Shimizu, T.
  • Organizer: Clostridia : The Impact of Genomics on Disease Control
  • Place of Presentation: ローマ高等衛生院(イタリア)
  • Year and Date: 2009-10-21
• [Presentation] ウェルシュ菌における細胞間情報伝達による毒素産生調節機構の解析 (2009)
  • Author(s): 大谷郁
  • Organizer: 第82回日本細菌学会総会
  • Place of Presentation: 名古屋国際会議場
  • Year and Date: 2009-03-14
• [Presentation] A群レンサ球菌の全ゲノム発現解析に基づく細胞内生存機構の解明 (2009)
  • Author(s): 大野雅幸
  • Organizer: 第82回日本細菌学会総会
  • Place of Presentation: 名古屋国際会議場
  • Year and Date: 2009-03-14
• [Presentation] オートファジー誘導によるA群レンサ球菌感染上皮の細胞死制御機構の解析 (2009)
  • Author(s): 相川知宏
  • Organizer: 第82回日本細菌学会総会
  • Place of Presentation: 名古屋国際会議場
  • Year and Date: 2009-03-14
• [Presentation] Analysis of regulatory network related to sopre formation in Clostridium perfringens (2009)
  • Author(s): Y. Yuan
  • Organizer: 第82回日本細菌学会総会
  • Place of Presentation: 名古屋国際会議場
  • Year and Date: 2009-03-13
• [Presentation] Effect of bacterial and host factors on gene expression in Clostridium perfringens (2007)
  • Author(s): Ohtani, K., Isu, A., Ohkubo, J., Shimizu, T.
  • Organizer: 107th General Meeting of American Society for Microbiology
  • Place of Presentation: トロント(カナダ)
  • Year and Date: 2007-05-22
• [Presentation] Biological signaling to gene expression in Clostridium perfringens (2006)
  • Author(s): Ohtani, K
  • Organizer: The 5th Clostpath 2006
  • Place of Presentation: Hugh Stewart Hall, Nottingham Univ., U.K.
  • Year and Date: 2006-06-24
• [Book] RNA実験ノート・下 (2008)
  • Author(s): 清水 徹
  • Total Pages: 133
  • Publisher: 羊土社
• [Remarks]
  • URL: https://www.genome-sci.jp/modules/contents2/rewrite/tc_76.html