| Project/Area Number |
17019051
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| Research Category |
Grant-in-Aid for Scientific Research on Priority Areas
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| Allocation Type | Single-year Grants |
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| Review Section |
Biological Sciences
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| Research Institution | Kyushu University |
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Principal Investigator |
TAHIRA Tomoko (2007-2009) Kyushu University, 生体防御医学研究所, 助教 (50155230)
林 健志 (2005-2006) 九州大学, 生体防御医学研究所, 教授 (00019671)
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| Co-Investigator(Kenkyū-buntansha) |
HAYASHI Kenshi 九州大学, 生体防御医学研究所, 特任教授 (00019671)
HORIUCHI Takahiko 九州大学, 大学病院, 准教授 (90219212)
KUKITA Yoji 大阪府立病院機構, 大阪府立成人病センター研究所, 研究員 (60372744)
HIGASA Koichiro 九州大学, 学術研究員 (10419583)
田平 知子 九州大学, 生体防御医学研究所, 助手 (50155230)
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| Project Period (FY) |
2005 – 2009
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| Project Status |
Completed (Fiscal Year 2009)
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| Budget Amount *help |
¥117,000,000 (Direct Cost: ¥117,000,000)
Fiscal Year 2009: ¥21,000,000 (Direct Cost: ¥21,000,000)
Fiscal Year 2008: ¥21,000,000 (Direct Cost: ¥21,000,000)
Fiscal Year 2007: ¥25,300,000 (Direct Cost: ¥25,300,000)
Fiscal Year 2006: ¥25,500,000 (Direct Cost: ¥25,500,000)
Fiscal Year 2005: ¥24,200,000 (Direct Cost: ¥24,200,000)
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| Keywords | SNP / 疾患関連解析 / 自己免疫疾患 / SSCP / ハプロタイプ / データベース / 関連解析 / PLACE-SSCP / 一塩基多型 / 確定ハプロタイプ / 全胞状奇胎 / QSNPlite / プールDNAによるゲノムワイド関連解析 / 日本人確定ハプロタイプ / DHaploDB / SLE / 家族性滲出性硝子体網膜症 / CpGアイランド / 一塩基多型(SNP) / SSCP解析 / 高精度アレル頻度決定 / ソフトウェア「QSNP lite」 / 胞状奇胎 / ゲノムワイドな確定ハプロタイプ / ゲノムワイドな関連解析 / 全身性エリテマトーデス(SLE) |
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| Research Abstract |
We have established the cost-effective and reliable pooling-based methods for genome-wide association study. This method screens disease-associated regions by determining allele frequencies of SNPs in pooled DNA first by microarray analysis and then by quantitative SSCP analysis. Using this method, we detected many disease-related genes of systemic lupus erythematosus. We also determined definitive haplotype consisting of SNPs and copy number polymorphisms by genotyping duplicated haploid genomes of complete hydatidiform moles. This linkage disequilibrium information of Japanese genome has been made available from "D-HaploDB".
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