| Project/Area Number |
17023021
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| Research Category |
Grant-in-Aid for Scientific Research on Priority Areas
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| Allocation Type | Single-year Grants |
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| Review Section |
Biological Sciences
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| Research Institution | The University of Tokyo (2007-2009)
Osaka University (2005-2006) |
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Principal Investigator |
KANO Masanobu The University of Tokyo, 大学院・医学系研究科, 教授 (40185963)
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| Co-Investigator(Kenkyū-buntansha) |
SAKIMURA Kenji 新潟大学, 脳研究所, 教授 (40162325)
OKABE Shigeo 東京大学, 大学院・医学系研究科, 教授 (60204012)
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| Project Period (FY) |
2005 – 2009
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| Project Status |
Completed (Fiscal Year 2009)
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| Budget Amount *help |
¥251,100,000 (Direct Cost: ¥251,100,000)
Fiscal Year 2009: ¥48,500,000 (Direct Cost: ¥48,500,000)
Fiscal Year 2008: ¥48,800,000 (Direct Cost: ¥48,800,000)
Fiscal Year 2007: ¥49,200,000 (Direct Cost: ¥49,200,000)
Fiscal Year 2006: ¥49,900,000 (Direct Cost: ¥49,900,000)
Fiscal Year 2005: ¥54,700,000 (Direct Cost: ¥54,700,000)
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| Keywords | 分子・細胞神経科学 / ニューロン・シナプス・神経回路 / 神経発生・神経発達・神経再生・神経再建 / 姿勢・運動制御 / 神経科学 / シグナル伝達 / シナプス / 発達 / 脳・神経 / 小脳 / プルキンエ細胞 / 登上線維 |
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| Research Abstract |
This research aimed at elucidating the mechanisms underlying strengthening of necessary synaptic connections and elimination of redundant ones during postnatal development of the brain. For this purpose, we used climbing fiber to Purkinje cell synapses in the cerebellum as a model system. We have disclosed that postnatal development of climbing fiber to Purkinje cell synapse consists of four distinct phases : (1)functional differentiation, (2)dendritic translocation of a single climbing fiber, (3)early phase of synapse elimination, (4)late phase of synapse elimination. We have elucidated part of the molecular mechanisms underlying each of the four phases. Moreover, we have established an organotipic coculture preparation of cerebellar slices with medulla explants containing the inferior olive. In this preparation, formation and elimination of climbing fiber synapses occur in a manner similar to those seen in vivo. This preparation can be used for screening candidate molecules that may be involved in the development of climbing synapses. Through the five years' research, we have achieved most of the original purposes.
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