Molecular Analysis of Brain System Controls under the Pure Genetic Background

• Project/Area Number: 17024011
• Research Category: Grant-in-Aid for Scientific Research on Priority Areas
• Allocation Type: Single-year Grants
• Review Section: Biological Sciences
• Research Institution: The University of Tokyo
• Principal Investigator: MISHINA Masayoshi The University of Tokyo, 大学院・医学系研究科, 教授 (80144351)
• Co-Investigator(Kenkyū-buntansha): UEMURA Takeshi 東京大学, 大学院・医学系研究科, 助教 (00372368) ; TAKEUCHI Tomonori 東京大学, 大学院・医学系研究科, 助教 (50323613)
• Project Period (FY): 2005 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥342,600,000 (Direct Cost: ¥342,600,000); Fiscal Year 2009: ¥61,600,000 (Direct Cost: ¥61,600,000); Fiscal Year 2008: ¥73,000,000 (Direct Cost: ¥73,000,000); Fiscal Year 2007: ¥73,400,000 (Direct Cost: ¥73,400,000); Fiscal Year 2006: ¥58,200,000 (Direct Cost: ¥58,200,000); Fiscal Year 2005: ¥76,400,000 (Direct Cost: ¥76,400,000)
• Keywords: 記憶 / 学習 / 情動 / 海馬 / 線条体 / 小脳 / 標的遺伝子組換え / 誘導型Cre組換え酵素 / 情動記憶 / 音刺激恐怖条件付け学習 / 長期記憶 / マウス / NMDA受容体 / medium spiny neuron / 運動学習 / シナプス可塑性 / グルタミン酸受容体 / PDZ蛋白質 / ノックアウトマウス / ネットワーク形成 / シナプス形成 / 神経回路網形成 / PDZ蛋白 / 運動失調 / 海馬CA3領域 / 反回性回路 / 脳領域特異的遺伝子ノックアウト / EEG spike / multiple unit activity / 同期発火 / グルタミン酸受容体GluRδ2 / 誘導型CrePRリコンビナーゼ / 小脳プルキニエ細胞 / シナプス結合 / 平行線維-プルキニエ細胞シナプス / アクティブゾーン / PSD

Research Abstract

We established C57BL/6 mouse lines expressing Cre or inducible CrePR recombinase genes under the control of hippocampus-, striatum- or cerebellum-specific promoters. By the use of the brain-region selective gene manipulation system, we have analyzed brain systems that control learning and memory. We found that the striatum is essential for the formation of fear memory under weak unconditioned stimulus. Furthermore, we showed that the facilitation of LTD induction at parallel fiber-Purkinje cell synapses enhances the optokinetic response adaptation, indicating that the synaptic plasticity at the specific site of a motor control circuit determines the efficacy of a motor learning.

Research Products

• [Journal Article] Trans-synaptic interaction of GluRδ2 and neurexin through Cbln1 mediates synapse formation in the cerebellum (2010)
  • Author(s): Uemura, T., Lee, S., Yasumura, M., Takeuchi, T., Yoshida, T., Ra, M., Taguchi, R., Sakimura, K., Mishina, M.
  • Journal Title: Cell (in press)
  • Peer Reviewed
• [Journal Article] Strain differences in stress responsivity are associated with divergent amygdala gene expression and glutamate-mediated neuronal excitability (2010)
  • Author(s): Mozhui, K., Karlsson, R-M., Kash, T., Ihne, J., Norcross, M., Patel, S., Farrell, M., Hill, E., Martin, K., Camp, M., Fitzgerald, P., Ciobanu, D., Sprengel, R., Mishina, M., Wellman, C., Winder, D., Williams, R.W., Holmes, A.
  • Journal Title: J. Neurosci. 30 Pages: 5357-5367
  • Peer Reviewed
• [Journal Article] Estrogen regulates the expression of N-methyl-D-aspartate (NMDA) receptor subunit epsilon 4 (Grin2d), that is essential for the normal sexual behavior in female mice (2010)
  • Author(s): Ikeda, K., Fukushima, T., Ogura, H., Tsukui, T., Mishina, M., Muramatsu, M., Inoue, S.
  • Journal Title: FEBS Lett 584 Pages: 806-810
  • Peer Reviewed
• [Journal Article] Estrogen regulates the expression of N-methyl-D-aspartate(NMDA)receptor subunit epsilon 4(Grin2d), that is essential for the normal sexual behavior in female mice (2010)
  • Author(s): Ikeda, K., Fukushima, T., Ogura, H., Tsukui, T., Mishina, M., Muramatsu, M., Inoue, S.
  • Journal Title: FEBS Lett. 584 Pages: 806-810
  • Peer Reviewed
• [Journal Article] Strain differences in stress responsivity are associated with divergent amygdala gene expression and glutamate-mediated neuronal excitability (2010)
  • Author(s): Mozhui, K., Karlsson, R-M., Kash, T., Ihne, J., Norcross, M., Patel, S., Farrell, M., Hill, E., Martin, K., Camp, M., Fitzgerald, P., Ciobanu, D., Sprengel, R., Mishina, M., Wellman, C., Winder, D., Williams, R.W., Holmes, A.
  • Journal Title: J.Neurosci. (印刷中(掲載確定))
  • Peer Reviewed
• [Journal Article] Trans-synaptic interaction of GluRδ2 and neurexin through Cbln1 mediates synapse formation in the cerebellum (2010)
  • Author(s): Uemura, T., Lee, S., Yasumura, M., Takeuchi, T., Yoshida, T., Ra, M., Taguchi, R., Sakimura, K., Mishina, M.
  • Journal Title: Cell (印刷中(掲載確定))
  • Peer Reviewed
• [Journal Article] Influence of parallel fiber-Purkinje cell synapse formation on postnatal development of climbing fiber-Purkinje cell synapses: Lessons from GluRδ2 knockout mice (2009)
  • Author(s): Hashimoto, K., Yoshida, T., Sakimura, K., Mishina, M., Watanabe, M., Kano, M.
  • Journal Title: Neuroscience 162 Pages: 601-611
  • Peer Reviewed
• [Journal Article] NR2A at CA1 synapses is obligatory for the susceptibility of hippocampal plasticity to sleep loss (2009)
  • Author(s): Longordo, F., Kopp, C., Mishina, M., Lujan, R., Luthi, A.
  • Journal Title: J. Neurosci. 29 Pages: 9026-9041
  • Peer Reviewed
• [Journal Article] Ablation of NMDA receptors enhances the excitability of hippocampal CA3 neurons (2009)
  • Author(s): Fukushima, F., Nakao, K., Shinoe, T., Fukaya, M., Muramatsu, S., Sakimura, K., Kataoka, H., Mori, H., Watanabe, M., Manabe, T., Mishina, M.
  • Journal Title: PLoS ONE 4
  • Peer Reviewed
• [Journal Article] A novel form of memory for auditory fear conditioning at a low-intensity unconditioned stimulus (2009)
  • Author(s): Kishioka, A., Fukushima, F., Ito, T., Kataoka, H., Mori, H., Ikeda, T., Itohara, S., Sakimura, K., Mishina, M.
  • Journal Title: PLoS ONE 4
  • Peer Reviewed
• [Journal Article] NR2A at CA1 synapses is obligatory for the susceptibility of hippocampal plasticity to sleep loss (2009)
  • Author(s): Longordo, F., Kopp, C., Mishina, M., Lujan, R., Luthi, A.
  • Journal Title: J.Neurosci. 29 Pages: 9026-9041
  • Peer Reviewed
• [Journal Article] Influence of parallel fiber-Purkinje cell synapse formation on postnatal development of climbing fiber-Purkinje cell synapses : Lessons from GluRδ2 knockout mice (2009)
  • Author(s): Hashimoto, K., Yoshida, T., Sakimura, K., Mishina, M., Watanabe, M., Kano, M.
  • Journal Title: Neuroscience 162 Pages: 601-611
  • Peer Reviewed
• [Journal Article] Regulation of synaptic vesicle accumulation and axon terminal remodeling during synapse formation by distinct Ca^<2+> signaling (2009)
  • Author(s): Yoshida, T., Uchida, S., Mishina, M.
  • Journal Title: J.Neurochem. 111 Pages: 160-170
  • Peer Reviewed
• [Journal Article] A novel form of memory for auditory fear conditioning at a low-intensity unconditioned stimulus (2009)
  • Author(s): Kishioka, A., Fukushima, F., Ito, T., Kataoka, H., Mori, H., Ikeda, T., Itohara, S., Sakimura, K., and Mishina, M.
  • Journal Title: PLoS ONE 4
  • Peer Reviewed
• [Journal Article] Ablation of NMDA receptors enhances the excitability of hippocampal CA3 neurons (2009)
  • Author(s): Fukushima, F., Nakao, K., Shinoe, T., Fukaya, M., Muramatsu, S., Sakimura, K., Kataoka, H., Mori, H., Watanabe, M., Manabe, T., and Mishina, M.
  • Journal Title: PLoS ONE 4
  • Peer Reviewed
• [Journal Article] Influence of parallel fiber-purkinje cell synapse formation on postnatal development of climbing fiber-purkinje cell synapses in the cerebellum (2009)
  • Author(s): Hashimoto, K., Yoshida, T., Sakimura, K., Mishina, M., Watanabe, M., and Kano, M.
  • Journal Title: Neuroscience (in pess)(掲載確定)(印刷中)
  • Peer Reviewed
• [Journal Article] The amino-terminal domain of glutamate receptorδ2 triggers presynaptic differentiation (2008)
  • Author(s): Uemura, T., Mishina, M.
  • Journal Title: Biochem. Biophys. Res. Commun. 377 Pages: 1315-1319
  • Peer Reviewed
• [Journal Article] Role of major NMDAR or AMPA receptor subunits in MK-801 potentiation of ethanol intoxication (2008)
  • Author(s): Palachick, B., Chen, Y. -C., Enoch, A.J., Karlsson, R. -M., Mishina, M., Holmes, A.
  • Journal Title: Alcohol. Clin. Exp. Res. 32 Pages: 1479-1492
  • Peer Reviewed
• [Journal Article] Enhancement of both long-term depression induction and optokinetic response adaptation in mice lacking Delphilin (2008)
  • Author(s): Takeuchi, T., Ohtsuki, G., Yoshida, T., Fukaya, M., Wainai, T., Yamashita, M., Yamazaki, Y., Mori, H., Sakimura, K., Kawamoto, S., Watanabe, M., Hirano, T., Mishina, M.
  • Journal Title: PLoS ONE 3
  • Peer Reviewed
• [Journal Article] Role of the internal Shank-binding segment of glutamate receptorδ2 in synaptic localization and cerebellar functions (2008)
  • Author(s): Yasumura, M., Uemura, T., Yamasaki, M., Sakimura, K., Watanabe, M., Mishina, M.
  • Journal Title: Neurosci. Lett. 433 Pages: 146-151
  • Peer Reviewed
• [Journal Article] Effects of FAK ablation on cerebellar foliation, Bergmann glia positioning and climbing fiber territory on Purkinje cells (2008)
  • Author(s): Watanabe, F., Miyazaki, T., Takeuchi, T., Fukaya, M., Nomura, T., Noguchi, S., Mori, H., Sakimura, K., Watanabe, M., Mishina, M.
  • Journal Title: Eur. J. Neurosci. 27 Pages: 836-854
  • Peer Reviewed
• [Journal Article] Differential regulation of synaptic plasticity and cerebellar motor learning by the C-terminal PDZ-binding motif of GluRδ2. (2008)
  • Author(s): Kakegawa, W., Miyzaki, T., Emi, K., Matsuda, K., Kohda, K., Motohashi, J., Mishina, M., Kawahara, S., Watanabe, M., Yuzaki, M.
  • Journal Title: J. Neurosci. 28 Pages: 1460-1468
  • Peer Reviewed
• [Journal Article] Impaired discrimination learning in mice lacking the NMDA receptor NR2A subunit. (2008)
  • Author(s): Brigman, J.L., Feyder, M., Saksida, L.M., Bussey, T.J., Mishina, M., Holmes, A.
  • Journal Title: Learn. Mem. 15 Pages: 50-54
  • Peer Reviewed
• [Journal Article] Enhancement of both long-term depression induction and optokinetic response adaptation in mice lacking Delphilin (2008)
  • Author(s): Takeuchi, T., Ohtsuki, G., Yoshida, T., Fukaya, M., Wainai, T., Yamashita, M., Yamazaki, Y., Mori, H., Sakimura, K., Kawamoto, S., Watanabe, M., Hirano, T. and Mishina, M.
  • Journal Title: PLoS ONE 3
  • Peer Reviewed
• [Journal Article] Role of major NMDAR or AMPA receptor subunits in MK-801 potentiation of ethanol intoxication (2008)
  • Author(s): Palachick, B., Chen, Y.-C., Enoch, A. J., Karlsson, R.-M., Mishina, M. and Holmes, A.
  • Journal Title: Alcoholism : Clinical and Experimental Research 32 Pages: 1479-1492
  • Peer Reviewed
• [Journal Article] The amino-terminal domain of glutamate receptor δ2 triggers presynaptic differentiation (2008)
  • Author(s): Uemura, T., and Mishina, M.
  • Journal Title: Biochem. Biophys. Res. Commun. 377 Pages: 1315-1319
  • Peer Reviewed
• [Journal Article] Differential regulation of synaptic plasticity and cerebellar motor learning by the C-terminal PDZ-binding motif of GluRδ2. (2008)
  • Author(s): Kakegawa, W., et. al.
  • Journal Title: J. Neurosci. 28 Pages: 1460-1468
  • Peer Reviewed
• [Journal Article] Effects of FAK ablation on cerebellar foliation, Bergmann glia positioning and climbing fiber territory on Purkinje cells. (2008)
  • Author(s): Watanabe, F., et. al.
  • Journal Title: Eur. J. Neurosci. 27 Pages: 836-854
  • Peer Reviewed
• [Journal Article] Impaired discrimination learning in mice lacking the NMDA receptor NR2A subunit. (2008)
  • Author(s): Brigman, J., et. al.
  • Journal Title: Learn. Mem. 15 Pages: 50-54
  • Peer Reviewed
• [Journal Article] Role of the internal Shank-binding segment of glutamate receptor δ2 in synaptic localization and cerebellar functions. (2008)
  • Author(s): Yasumura, M., et. al.
  • Journal Title: Neurosci. Lett. 433 Pages: 146-151
  • Peer Reviewed
• [Journal Article] Regulation of long-term depression and climbing fiber territory by glutamate receptorδ2 at parallel fiber synapses through its C-terminal domain in cerebellar Purkinje cells (2007)
  • Author(s): Uemura, T., Kakizawa, S., Yamasaki, M., Sakimura, K., Watanabe, M., Iino, M., Mishina, M.
  • Journal Title: J. Neurosci. 27 Pages: 12096-12108
  • Peer Reviewed
• [Journal Article] Activation of NMDA receptors promotes dendritic spine development through MMP-mediated ICAM-5 cleavage (2007)
  • Author(s): Tian, L., Stefanidakis, M., Ning, L., Lint, P.V., Nyman-Huttunen, H., Libert, C., Itohara, S., Mishina, M., Rauvala, H., Gahmberg, C.G.
  • Journal Title: J. Cell Biol. 178 Pages: 687-700
  • Peer Reviewed
• [Journal Article] Nitric oxide (NO) serves as a retrograde messenger to activated neuronal NO synthase in the spinal cord via NMDA receptors (2007)
  • Author(s): Xu, L., Mabuchi, T., Katano, T., Matsumura, S., Okuda-Ashitaka, E., Sakimura, K., Mishina, M., Ito, S.
  • Journal Title: Nitric Oxide 17 Pages: 18-24
  • Peer Reviewed
• [Journal Article] Regulation of dendritogenesis via a lipid raft-associated Ca2+/calmodulin-dependent protein kinase CLICK-III/CaMKIγ (2007)
  • Author(s): Takemoto-Kimura, S., Ishihara-Ageta, N., Nonaka, M., Adachi-Morishima, A., Mano, T., Okamura, M., Fujii, H., Fuse, T., Hoshino, M., Suzuki, S., Kojima, M., Mishina, M., Okuno, H., Bito, H.
  • Journal Title: Neuron 54 Pages: 755-770
  • Peer Reviewed
• [Journal Article] Conditional gene targeting on the pure C57BL/6 genetic background (2007)
  • Author(s): Mishina, M., Sakimura, K.
  • Journal Title: Neurosci. Res. 58 Pages: 105-112
  • Peer Reviewed
• [Journal Article] Mouse liaison for integrative brain research (2007)
  • Author(s): Aiba, A., Inokuchi, K., Ishida, Y., Itohara, S., Kobayashi, K., Masu, M., Mishina, M., Miyakawa, T., Mori, H., Nakao, K., Obata, Y., Sakimura, K., Shirosihi, T., Wada, K., Yagi, T.
  • Journal Title: Neurosci. Res. 58 Pages: 103-104
  • Peer Reviewed
• [Journal Article] Enhanced cell-to-cell contacts between activated microglia and pyramidal cell dendrites following kainic acid-induced neurotoxicity in the hippocampus (2007)
  • Author(s): Hasegawa, S., Yamaguchi, M., Nagao, H., Mishina, M., Mori, K.
  • Journal Title: J. Neuroimmunol. 186 Pages: 75-85
  • Peer Reviewed
• [Journal Article] Signal pathways coupled to activation of neuronal nitric oxide synthase in the spinal cord by nociceptin/orphanin FQ (2007)
  • Author(s): Xu, L., Okuda-Ashitaka, E., Matsumura, S., Mabuchi, T., Okamoto, S., Sakimura, K., Mishina, M., Ito, S.
  • Journal Title: Neuropharmacology 52 Pages: 1318-1325
  • Peer Reviewed
• [Journal Article] Ca2+ permeability of the channel pore is not essential for theδ2 glutamate receptor channel to regulate synaptic plasticity and motor coordination (2007)
  • Author(s): Kakegawa, W., Miyazaki, T., Hirai, H., Motohashi, J., Mishina, M., Watanabe, M., Yuzaki, M.
  • Journal Title: J. Physiol. 579 Pages: 729-735
  • Peer Reviewed
• [Journal Article] Ca^<2+>permeability of the channel pore is not essential for the δ2 glutamate receptor channel to regulate synaptic plasticity and motor coordination. (2007)
  • Author(s): Kakegawa, W., et. al.
  • Journal Title: J. Physiol. 579 Pages: 729-735
  • Peer Reviewed
• [Journal Article] Signal pathways coupled to activation of neuronal nitric oxide synthase in the spinal cord by nociceptin/orphanin FQ. (2007)
  • Author(s): Xu, L., et. al.
  • Journal Title: Neuropharmacology 52 Pages: 1318-1325
  • Peer Reviewed
• [Journal Article] Mouse liaison for integrative brain research. (2007)
  • Author(s): Aiba, A., et. al.
  • Journal Title: Neurosci. Res. 58 Pages: 103-104
• [Journal Article] Conditional gene targeting on the pure C57BL/6 genetic background. (2007)
  • Author(s): Mishina, M. and Sakimura, K.
  • Journal Title: Neurosci. Res. 58 Pages: 105-112
  • Peer Reviewed
• [Journal Article] Enhanced cell-to-cell contacts between activated microglia and pyramidal cell dendrites following kainic acid-induced neurotoxicity in the hippocampus. (2007)
  • Author(s): Hasegawa, S., et. al.
  • Journal Title: J. Neuroimmunol. 186 Pages: 75-85
  • Peer Reviewed
• [Journal Article] Regulation of dendritogenesis via a lipid raft-associated Ca^<2+>/calmodulin-dependent protein kinase CLICK-III/CaMKIy. (2007)
  • Author(s): Takemoto-Kimura, S., et. al.
  • Journal Title: Neuron 54 Pages: 755-770
  • Peer Reviewed
• [Journal Article] Nitric oxide (NO) serves as a retrograde messenger to activate neuronal NO synthase in the spinal cord via NMDA receptors. (2007)
  • Author(s): Xu, L., et. al.
  • Journal Title: Nitric Oxide 17 Pages: 18-24
  • Peer Reviewed
• [Journal Article] Activation of NMDA receptors promotes dendritic spine development through MMP-mediated ICAM-5 cleavage. (2007)
  • Author(s): Tian, L., et. al.
  • Journal Title: J. Cell Biol. 178 Pages: 687-700
  • Peer Reviewed
• [Journal Article] Regulation of long-term depression and climbing fiber territory by glutamate receptor δ2 at parallel fiber synapses through its C-terminal domain in cerebellar Purkinje cells. (2007)
  • Author(s): Uemura, T., et. al.
  • Journal Title: J. Neurosci. 27 Pages: 12096-12108
  • Peer Reviewed
• [Journal Article] Ca^<2+> permeability of the channel pore is not essential for the δ2 glutamate receptor channel to regulate synaptic plasticity and motor coordination. (2007)
  • Author(s): Kakegawa, W., Miyzaki, T., Hirai H., Motohashi, J., Mishina, M., Watanabe, M., Yuzaki, M.
  • Journal Title: J. Physiol. 579 Pages: 729-735
• [Journal Article] Signal pathways coupled to activation of neuronal nitric oxide synthase in the spinal cord by nociceptin/orphanin FQ. (2007)
  • Author(s): Xu, L., Okuda-Ashitaka, E., Matsumura, S., Mabuchi, T., Okamoto S., Sakimura, k., Mishina, M., Ito, S.
  • Journal Title: Neuropharmacology. 52 Pages: 1318-1325
• [Journal Article] Mouse liaison for integrative brain research. (2007)
  • Author(s): Aiba, A., Inokuchi, K., Ishida, Y., Itohara, S., Kobayashi, K., Masu, M., Mishina, M., Miyakawa, T., Mori, H., Nakao, k., Obata Y., Sakimura, K., Shiroishi, T., Wada, k., Yagi, T.
  • Journal Title: Neurosci Res. 58(in press)
• [Journal Article] Conditional gene targeting on the pure C57BL/6 genetic background. (2007)
  • Author(s): Mishina, M., Sakimura, K.
  • Journal Title: Neurosci. Res. 58(in press)
• [Journal Article] Dietary restriction increases hippocampal neurogenesis by molecular mechanisms independent of NMDA receptors (2006)
  • Author(s): Kitamura, T., Mishina, M., Sugiyama, H.
  • Journal Title: Neurosci. Lett. 393 Pages: 94-96
  • Peer Reviewed
• [Journal Article] Telencephalin slows spine maturation (2006)
  • Author(s): Matsuno, H., Okabe, S., Mishina, M., Yanagida, T., Mori, K., Yoshihara, Y.
  • Journal Title: J. Neurosci. 26 Pages: 1776-1786
  • Peer Reviewed
• [Journal Article] Telencephalin slows spine maturation. (2006)
  • Author(s): Matsuno, H., Okabe, S., Mishina, M., Yanagida, T., Mori, K., Yoshihara, Y.
  • Journal Title: J. Neurosci. 26 Pages: 1776-1786
• [Journal Article] Dietary restriction increases hippocampal neurogenesis by molecular mechanisms independent of NMDA receptors. (2006)
  • Author(s): Kitamura, T., Mishina, M., Sugiyama, H
  • Journal Title: Neurosci. Lett. 393 Pages: 94-96
• [Journal Article] Telencephalin slows spine maturation. (2006)
  • Author(s): Matsuno, H., Okabe, S., Mishina, M., Yanagida, T., Mori, K., Yoshihara, Y.
  • Journal Title: J.Neurosci. 26 Pages: 1776-1786
• [Journal Article] Characterization of hippocampal theta rhythm in wild-type mice and glutamate receptor subunitδ2 mutant mice during eyeblink conditioning with a short trace interval (2005)
  • Author(s): Takatsuki, K., Kawahara, S., Mishina, M., Kirino, Y.
  • Journal Title: Brain Res. 1063 Pages: 159-167
  • Peer Reviewed
• [Journal Article] N-methyl-D-aspartate receptors play important roles in acquisition and expression of the eyeblink conditioned response in glutamate receptor subunitδ2 mutant mice (2005)
  • Author(s): Kato, Y., Takatsuki, K., Kawahara, S., Fukunaga, S., Mori, H., Mishina, M., Kirino, Y.
  • Journal Title: Neuroscience 135 Pages: 1017-1023
  • Peer Reviewed
• [Journal Article] Identification and characterization of a novel Delphilin variant with an alternative N-terminus (2005)
  • Author(s): Yamashita, T., Miyagi, Y., Ono, M., Ito, H., Watanabe, K., Sonoda, T., Tsuzuki, K., Ozawa, S., Aoki, I., Okuda, K., Mishina, M., Kawamoto, S.
  • Journal Title: Mol. Brain Res. 141 Pages: 83-95
  • Peer Reviewed
• [Journal Article] Target-cell-specific left-right asymmetry of NMDA receptor content in Schaffer collateral synapses inε1/NR2A knockout mice (2005)
  • Author(s): Wu, Y., Kawakami, R., Fukaya, M., Sakimura, K., Mishina, M., Watanabe, M., Ito, I., Shigemoto, R.
  • Journal Title: J. Neurosci. 25 Pages: 9213-9226
  • Peer Reviewed
• [Journal Article] Fyn-kinase-mediated phosphorylation of NMDA receptor NR2B subunit at Tyr 1472 is essential for maintenance of neuropathic pain (2005)
  • Author(s): Abe, T., Matsumura, S., Katano, T., Mabuchi, T., Takagi, K., Xu, L., Yamamoto, A., Hattori, K., Yagi, T., Watanabe, M., Nakazawa, T., Yamamoto, T., Sasaki, Y., Suzuki, M., Mishina, M., Nakai Y., Ito, S.
  • Journal Title: Eur. J. Neurosci. 22 Pages: 1445-1454
  • Peer Reviewed
• [Journal Article] Activation of NR2A-containing NMDA receptors is not obligatory for NMDA-receptor dependent long-term potentiation (2005)
  • Author(s): Weitlauf, C., Honse, Y., Auberson, Y.P., Mishina, M., Lovinger, D.M., Winder, D.G.
  • Journal Title: J. Neurosci. 25 Pages: 8386-8390
  • Peer Reviewed
• [Journal Article] Effect of N-methyl-D-aspartate receptorε1 subunit gene disruption of the action of general anesthetic drugs in mice (2005)
  • Author(s): Sato, Y., Kobayashi, E., Murayama, T., Mishina, M., Seo, N.
  • Journal Title: Anesthesiology 102 Pages: 557-561
  • Peer Reviewed
• [Journal Article] Defective control and adaptation of reflex eye movements in mutant mice deficient in either the glutamate receptorδ2 subunit or Purkinje cells (2005)
  • Author(s): Katoh, A., Yoshida, T., Himeshima, Y., Mishina, M., Hirano, T.
  • Journal Title: Eur. J. Neurosci. 21 Pages: 1315-1326
  • Peer Reviewed
• [Journal Article] Role of hippocampal NMDA receptors in trace eyeblink conditioning (2005)
  • Author(s): Sakamoto, T., Takatsuki, K., Kawahara, S., Kirino, Y., Niki, H., Mishina, M.
  • Journal Title: Brain Res. 1039 Pages: 130-136
  • Peer Reviewed
• [Journal Article] Control of synaptic connection by glutamate receptorδ2 in the adult cerebellum (2005)
  • Author(s): Takeuchi, T., Miyazaki, T., Watanabe, M., Mori, H., Sakimura, K., Mishina, M.
  • Journal Title: J. Neurosci. 25 Pages: 2146-2156
  • Peer Reviewed
• [Journal Article] Rescue of abnormal phenotypes of theδ2 glutamate receptor-null mice by mutantδ2 transgenes (2005)
  • Author(s): Hirai, H., Miyazaki, T., Kakegawa, W., Matsuda, S., Mishina, M., Watanabe, M., Yuzaki, M.
  • Journal Title: EMBO Reports 6 Pages: 90-95
  • Peer Reviewed
• [Journal Article] Rescue of abnormal phenotypes of the δ2 glutamate receptor-null mice by mutant δ2 transgenes. (2005)
  • Author(s): Hirai, H., Miyazaki, T., Kakegawa, W., Matsuda S., Mishina, M., Watanabe, M., Yuzaki, M.
  • Journal Title: EMBO Report 6 Pages: 90-95
• [Journal Article] Control of synaptic connection by glutamate receptor δ2 in the adult cerebellum. (2005)
  • Author(s): Takeuchi, T., Miyazaki, T., Watanabe, M., Mori, H., Sakimura, K., Mishina, M.
  • Journal Title: J.Neurosci. 25 Pages: 2146-2156
• [Journal Article] Distinct roles of calcineurin-nuc lear factor o activated T-cells and protein kinase A-cAMP response element binding protein signaling in presynaptic differentiation. (2005)
  • Author(s): Yoshida, T., Mishina, M.
  • Journal Title: J.Neurosci. 25 Pages: 3067-3079
• [Journal Article] Role of hippocampal NMDA receptors in trace eyeblink conditioning. (2005)
  • Author(s): Sakamoto, T., Takatsuki, K., Kawahara, S., Kirino, Y., Niki, H., Mishina, M.
  • Journal Title: Brain Res. 1039 Pages: 130-136
• [Journal Article] Defective control and adaptation of reflex eye movements in mutant mice deficient in either the glutamate receptor δ2 subunit or Purkinje cells. (2005)
  • Author(s): Katoh, A., Yoshida, T., Himeshima, Y., Mishina, M., Hirano, T.
  • Journal Title: Eur.J.Neurosci. 21 Pages: 1315-1326
• [Journal Article] Effect of N-methyl-D-aspartate receptor ε1 subunit gene disruption of the action of general anesthetic drugs in mice. (2005)
  • Author(s): Sato, Y., Kobayashi, E., Murayama, T., Mishina, M., Seo, N.
  • Journal Title: Anesthesiology 102 Pages: 557-561
• [Journal Article] Autoantibodies and cell-mediated autoimmunity to NMDA-type GluRε2 in patients with Rasmussen's encephalitis and chronic progressive epilepsia partialis continua. (2005)
  • Author(s): Takahashi, Y., Mori, H., Mishina, M., Watanabe, M., Kondo, N., Shimomura, J., Kubota, Y., Matsuda, K., Fukushima, K., Shiroma, N., Akasaka, N., Nishida, H., Imamura A., Watanabe, H., Sugiyama, N., Ikezawa, M., Fujiwara, T.
  • Journal Title: Epilepsia 46(Suppl.5) Pages: 152-158
• [Journal Article] Identification and characterization of a novel member of murine semaphorin family. (2005)
  • Author(s): Taniguchi, M., Masuda T., Fukaya, M., Kataoka, H., Mishina, M., Yaginuma H., Watanabe, M., Shimizu, T.
  • Journal Title: Genes Cells 10 Pages: 785-792
• [Journal Article] Activation of NR2A-containing NMDA receptors is not obligatory for NMDA-receptor dependent long-term potentiation. (2005)
  • Author(s): Weitlauf, C., Honse, Y., Auberson, Y.P., Mishina, M., Lovinger, D.M., Winder, D.G.
  • Journal Title: J.Neurosci. 25 Pages: 8386-8390
• [Journal Article] Fyn-kinase-mediated phosphorylation of NMDA receptor NR2B subunit at Tyr 1472 is essential for maintenance of neuropathic pain. (2005)
  • Author(s): Abe, T., Matsumura, S., Katano, T., Mabuchi, T., Takagi, K., Xu L., Yamamoto A., Hattori, K., Yagi, T., Watanabe, M., Nakazawa, T., Yamamoto, T., Sasaki, Y., Suzuki, M., Mishina, M., Nakai Y., Ito, S.
  • Journal Title: Eur.J.Neurosci. 22 Pages: 1445-1454
• [Journal Article] Target-cell-specific left-right asymmetry of NMDA receptor content in Schaffer collateral synapses in ε1/NR2A knockout mice. (2005)
  • Author(s): Wu, Y., Kawakami, R., Fukaya, M., Sakimura, K., Mishina, M., Watanabe, M., Ito, I., Shigemoto, R.
  • Journal Title: J.Neurosci. 25 Pages: 9213-9226
• [Journal Article] Identification and characterization of a novel Delphilin variant with an alternative N-terminus. (2005)
  • Author(s): Yamashita, T., Miyagi, Y., Ono, M., Ito, H., Watanabe, K., Sonoda, T., Tsuzuki, K., Ozawa, S., Aoki, I., Okuda, K., Mishina, M., Kawamoto, S.
  • Journal Title: Mol.Brain Res. 141 Pages: 83-95
• [Journal Article] N-methyl-D-aspartate receptors play important roles in acquisition and expression of the eyeblink conditioned response in glutamate receptor subunit δ2 mutant mice. (2005)
  • Author(s): Kato, Y., Takatsuki, K., Kawahara, S., Fukunaga, S., Mori, H., Mishina, M., Kirino, Y.
  • Journal Title: Neuroscience 135 Pages: 1017-1023
• [Journal Article] Characterization of hippocampal theta rhythm in wild-type mice and glutamate receptor subunit δ2 mutant mice during eyeblink conditioning with a short trace interval. (2005)
  • Author(s): Takatsuki K., Kawahara S., Mishina, M., Kirino, Y.
  • Journal Title: Brain Res. 1063 Pages: 159-167
• [Presentation] A study of the role of the layer 4 of the cerebral cortex in fear memory (2010)
  • Author(s): N.Endo, T.Uemura, K.Sakimura, S.Itohara, M.Mishina
  • Organizer: The 83^<nd> Annual Meeting of the Japanese Pharmacological Society
  • Place of Presentation: Osaka International Convention Center, Osaka, Japan
  • Year and Date: 2010-03-18
• [Presentation] Control of synaptic connection by Cbln1 in the adult cerebellum (2010)
  • Author(s): M.Yasumura, T.Takeuchi, T.Uemura, K.Sakimura, M.Mishina
  • Organizer: The 83^<nd> Annual Meeting of the Japanese Pharmacological Society
  • Place of Presentation: Osaka International Convention Center, Osaka, Japan
  • Year and Date: 2010-03-17
• [Presentation] Identification of Cbln1 as a novel ligand for glutamate receptor δ2 (2010)
  • Author(s): T.Uemura, S-J.Lee, M.Yasumura, T.Yoshida, M.Ra, R.Taguchi, M.Mishina
  • Organizer: The 83^<nd> Annual Meeting of the Japanese Pharmacological Society
  • Place of Presentation: Osaka International Convention Center, Osaka, Japan
  • Year and Date: 2010-03-17
• [Presentation] High-affinity and selective binding of Cbln1 to glutamate receptor δ2 (2010)
  • Author(s): S.J.Lee, T.Uemura, M.Mishina
  • Organizer: The 83^<nd> Annual Meeting of the Japanese Pharmacological Society
  • Place of Presentation: Osaka International Convention Center, Osaka, Japan
  • Year and Date: 2010-03-17
• [Presentation] Establishment of a mouse line for inducible ablation of neurons in the layer 4 of the cerebral cortex (2009)
  • Author(s): N.Endo, T.Uemura, K.Sakimura, S.Itohara, M.Mishina
  • Organizer: The 32^<nd> Annual Meeting of the Japan Neuroscience Society
  • Place of Presentation: Nagoya Congress Center, Nagoya, Japan
  • Year and Date: 2009-09-18
• [Presentation] Effects of inducible ablation of cerebellar granule or Purkinje cells on motor control (2009)
  • Author(s): E.Kato, T.Uemura, X.Chen, F.Watanabe, M.Yasumura, S.Itohara, K.Sakimura, M.Mishina
  • Organizer: The 32^<nd> Annual Meeting of the Japan Neuroscience Society
  • Place of Presentation: Nagoya Congress Center, Nagoya, Japan
  • Year and Date: 2009-09-18
• [Presentation] The role of the N-terminal domain of glutamate receptor δ2 in synapse formation (2009)
  • Author(s): T.Uemura, M.Mishina
  • Organizer: The 32^<nd> Annual Meeting of the Japan Neuroscience Society
  • Place of Presentation: Nagoya Congress Center, Nagoya, Japan
  • Year and Date: 2009-09-17
• [Presentation] GruRε2(NR2B)is specifically involved in the corticospinal synapse elimination during development (2009)
  • Author(s): T.Ohno, H.Maeda, N.Murabe, T.Kamiyama, N.Yoshioka, M.Mishina, M.Sakurai
  • Organizer: The 32^<nd> Annual Meeting of the Japan Neuroscience Society
  • Place of Presentation: Nagoya Congress Center, Nagoya, Japan
  • Year and Date: 2009-09-16
• [Presentation] Role of the C-terminal PDZ binding domain of glutamate receptor 82 in synaptic plasticity and climbing fiber wiring (2008)
  • Author(s): T. Uemura, S. Kakizawa, M. Yamasaki, K. Sakimura, M. Watanabe, M. Iino and M. Mishina
  • Organizer: The 31^<th> Annual Meeting of the Japan Neuroscience Society
  • Place of Presentation: Tokyo International Forum. Japan
  • Year and Date: 2008-07-11
• [Presentation] Role of the internal Shank-binding segment of GluR82 in cerebellar functions (2008)
  • Author(s): M. Yasumura, T. Uemura, M. Yamasaki, K. Sakimura, M. Watanabe and M. Mishina
  • Organizer: The 31^<th> Annual Meeting of the Japan Neuroscience Society
  • Place of Presentation: Tokyo International Forum Japan
  • Year and Date: 2008-07-11
• [Presentation] Auditory fear memory requires the striatal neurons under weak conditioning (2008)
  • Author(s): A Kishioka, F Fukushima, T. Ito, H. Kataoka, H. Mori, T. Ikeda, S. Itohara, K. Sakimura and M. Mishina
  • Organizer: The 31^<th> Annual Meeting of the Japan Neuroscience Society
  • Place of Presentation: Tokyo International Forum Japan
  • Year and Date: 2008-07-10
• [Presentation] The ectopic location of Bergmann glia and altered climbing fiber territory on Purkinje cells by FAK ablation (2008)
  • Author(s): F Watanabe, T. Miyazaki, T. Takeuchi, K. Sakimura, M. Watanabe and M. Mishina
  • Organizer: The 31^<th> Annual Meeting of the Japan Neuroscience Society
  • Place of Presentation: Tokyo International Forum, Japan
  • Year and Date: 2008-07-10
• [Presentation] Motor dysfunction in neostriatal vesicular GABA transporter knock out mice (2008)
  • Author(s): K. Saito, T. Kakizaki, T. Momiyama, H. Kataoka, M. Mishina and Y. Yanaaawa
  • Organizer: The 31^<th> Annual Meeting of the Japan Neuroscience Society
  • Place of Presentation: Tokyo International Forum, Japan
  • Year and Date: 2008-07-09
• [Presentation] From glutamate receptors to brain dynamics. (2008)
  • Author(s): Mishina, M.
  • Organizer: The 81st Annual Meeting of the Japanese Pharmacological Society
  • Place of Presentation: Yokohama, Japan
  • Year and Date: 2008-03-19
• [Presentation] Auditory fear memory requires the striatal neurons under weak conditioning. (2008)
  • Author(s): Kishioka, A., et. al.
  • Organizer: The 81st Annual Meeting of the Japanese Pharmacological Society
  • Place of Presentation: Yokohama, Japan
  • Year and Date: 2008-03-17
• [Presentation] The C-terminal domain of GluRδ2 is essential for cerebellar LTD and regulation of Climbing fiber territory at parallel fiber synapses in cerebellar Purkinje cells (2008)
  • Author(s): Uemura, T., et. al.
  • Organizer: The 81st Annual Meeting of the Japanese Pharmacological Society
  • Place of Presentation: Yokohama, Japan
  • Year and Date: 2008-03-17
• [Presentation] Retardations in cerebellar foliation, the ectopic lacation of Bergmann glia, and altered climbing fiber territory on Pukinje cells by FAK abration. (2008)
  • Author(s): Watanabe, F., et. al.
  • Organizer: The 81st Annual Meeting of the Japanese Pharmacological Society
  • Place of Presentation: Yokohama, Japan
  • Year and Date: 2008-03-17
• [Presentation] Viral vector-mediated ablation of the hippocampal CA3 NMDA receptors in the mature mice generated large-amplitude spike activities. (2008)
  • Author(s): Fukushima, F., et. al.
  • Organizer: The 81st Annual Meeting of the Japanese Pharmacological Society
  • Place of Presentation: Yokohama, Japan
  • Year and Date: 2008-03-17
• [Presentation] Studies on glutamate receptor functions in learning and neural wiring by region-specific and inducible ablation. (2007)
  • Author(s): Mishina, M.
  • Organizer: 10th Southeast Asian Western Pacific Regional Meeting of Pharmacologists / 41st Annual Scientific Meeting of the Australasian Society of Clinical and Experimental Pharmacologists and Toxicologists
  • Place of Presentation: Adelaide, Australia
  • Year and Date: 2007-12-03
• [Presentation] Regulation of climbing fiber territory by the carboxyl terminal of GluRδ2 at parallel fiber synapses in cerebellar Purkinje cells. (2007)
  • Author(s): Uemura, T., et. al.
  • Organizer: Society for Neuroscience 37th Annual Meeting
  • Place of Presentation: San Diego, USA
  • Year and Date: 2007-11-06
• [Presentation] GluRε2[2B] dependent synapse elimination of corticospinal tract in mice. (2007)
  • Author(s): Ohno, T., et. al.
  • Organizer: The 30th Annual Meeting of the Japan Neuroscience Society
  • Place of Presentation: Yokohama, Japan
  • Year and Date: 2007-09-11
• [Presentation] Accelerated maturation of visual response properties in telencephalin knockout mice. (2007)
  • Author(s): Fagiolini, M., et. al.
  • Organizer: The 30th Annual Meeting of the Japan Neuroscience Society
  • Place of Presentation: Yokohama, Japan
  • Year and Date: 2007-09-11