Mechanism of translation-coupled mRNA decay
| Project/Area Number |
17026004
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| Research Category |
Grant-in-Aid for Scientific Research on Priority Areas
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| Allocation Type | Single-year Grants |
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| Review Section |
Biological Sciences
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| Research Institution | Nagoya City University |
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Principal Investigator |
HOSHINO Shinichi Nagoya City University, Graduate School of Pharmaceutical Sciences, Professor (40219168)
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| Project Period (FY) |
2005 – 2006
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| Project Status |
Completed (Fiscal Year 2006)
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| Budget Amount *help |
¥14,200,000 (Direct Cost: ¥14,200,000)
Fiscal Year 2006: ¥7,100,000 (Direct Cost: ¥7,100,000)
Fiscal Year 2005: ¥7,100,000 (Direct Cost: ¥7,100,000)
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| Keywords | genetics / gene expression / nucleic acids / enzyme |
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| Research Abstract |
In this study, we have investigated the initiation mechanism of mRNA decay. Control of mRNA decay is a fundamentally important step in determining the amount of protein produced from the mRNA by translation, and mRNA decay is intimately linked to and regulated by translation. In eukaryotes, decay of most mRNAs is initiated by shortening of the poly(A)-tail at the 3' end, referred to as deadenylation. Deadenylation is the first and rate-limiting step and also the most efficient step in controlling mRNA decay. Two major deadenylase complexes, Caf1-Ccr4 and Pan2-Pan3, play central roles in this process. However, the molecular mechanism triggering deadenylation remained elusive. We previously showed that translation termination factor eRF3 interacts with poly(A) binding protein (PABPC1) through its N-domain and that eRF3 regulates the initiation of mRNA decay at the poly(A) tail shortening step through the interaction with PABPC1 in a manner coupled to translation termination. Thus, transl
… More
ation termination triggers mRNA decay. Here we examined the mechanism of mRNA deadenylation and obtained evidence fir a molecular interplay between translation termination factor eRF3 and mRNA deadenylases. We have demonstrated that eRF3-mediated deadenylation is catalyzed by both of the two major mRNA deadenylase complexes, Caf1-Ccr4 and Pan2-Pan3, in yeast and humans. Interestingly, translation termination complex eRF1-eRF3, Pan2-Pan3 and Caf1-Ccr4 competitively bind to the PABC domain of PABPC1. In each complex, eRF3, Pan3 and Tob, respectively, mediate PABPC1-binding. The PAM2 motifs found in both eRF3 and the two deadenylase complexes are responsible both for their binding to PABPC1 and for mRNA deadenylation. The termination complex and the deadenylase complex are exchanged on PABPC1 in a translation-dependent manner. Recruitment of the two deadenylase complexes to PABPC1 leads to the activation of both enzymes. From these results, we suggest a mechanism of mRNA deadenylation by Pan2-Pan3 and Caf1-Ccr4 in cooperation with eRF3 and PABPC1. Less
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Report
(3 results)
Research Products
(37 results)
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[Journal Article] Mechanism of mRNA deadenylation : evidence for a molecular interplaybetween translation termination factor eRF3 and mRNA deadenylases.2007
Author(s)
Funakoshi, Y., Doi, Y., Hosoda, N., Uchida, N., Osawa, M., Shimada, I., Tsujimoto, M., Suzuki, T., Katada, T., Hoshino, S.
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Journal Title
Genes & Development 21
Pages: 3135-3148
Description
「研究成果報告書概要(和文)」より
Related Report
Peer Reviewed
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[Journal Article] LARK activates posttranscriptional expression of an essential mammalian clock protein, PERIOD1.2007
Author(s)
Kojima, S., Matsumoto, K., Hirose, M., Shimada, M., Nagano, M., Shigeyoshi, Y., Hoshino, S., Tei, K., Saigo, K., Green, CB., Sakaki, Y., Tei, H
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Journal Title
Proc. Natl. Acad. Sci 104
Pages: 1859-1864
Description
「研究成果報告書概要(和文)」より
Related Report
Peer Reviewed
-
[Journal Article] Mechanism of mRNA deadenylation : evidence for a molecular interplay between translation termination factor eRF3 and mRNA deadenylases.2007
Author(s)
Funakoshi, Y., Doi, Y., Hosoda, N., Uchida, N., Osawa, M., Shimada, I., Tsujimoto, M., Suzuki, T., Katada, T., Hoshino, S.
-
Journal Title
Genes Dev 21
Pages: 3135-3148
Description
「研究成果報告書概要(欧文)」より
Related Report
-
[Journal Article] LARK activates posttranscriptional expression of an essential mammalian clock protein, PERIOD1.2007
Author(s)
Kojima, S., Matsumoto, K., Hirose, M., Shimada, M., Nagano, M., Shigeyoshi, Y., Hoshino, S., Ui-Tei, K., Saigo, K., Green, C. B., Sakaki, Y., Tei, H.
-
Journal Title
Proc Natl Acad Sci U S A 104
Pages: 1859-1864
Description
「研究成果報告書概要(欧文)」より
Related Report
-
[Journal Article] LARK activates posttranscriptional expression of an essential mammalian clock protein, PERIOD1.2007
Author(s)
Kojima, S., Matsumoto, K., Hirose, M., Shimada, M., Nagano, M., Shigeyoshi, Y., Hoshino, S., Tei, K., Saigo, K., Green, CB., Sakaki, Y., Tei, H
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Journal Title
Proc.Natl.Acad.Sci. 104
Pages: 1859-1859
Related Report
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[Journal Article] Binding of a novel SMG-1-Upf1-eRF1-eRF3 complex (SURF)to the exon junction complex triggers Upf1 phosphorylation and nonsense・mediatedmRNA decay.2006
Author(s)
Kashima, I., Yamashita, A., Izumi, N., Kataoka, N., Morishita, R., Hoshino, S., Ohno, M., Dreyfuss, G., Ohno, S
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Journal Title
Genes & Development 20
Pages: 355-367
Description
「研究成果報告書概要(和文)」より
Related Report
Peer Reviewed
-
[Journal Article] The decapping enzyme Dcp1 participates in translation termination through its interaction with the release factor eRF3 in budding yeast.2006
Author(s)
Kofuji, S., Sakuno, T., Takahashi, S., Araki, Y., Doi, Y., Hoshinoi, S., Katada, T.
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Journal Title
Biochem Biophys Res Commun 344
Pages: 547-553
Description
「研究成果報告書概要(和文)」より
Related Report
Peer Reviewed
-
[Journal Article] The decapping enzyme Dcpl participates in translation termination through its interaction with the release factor eRF3 in budding yeast.2006
Author(s)
Kofuji, S., Sakuno, T., Takahashi, S., Araki, Y., Doi, Y., Hoshino, S., Katada, T.
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Journal Title
Biochem Biophys Res Commun 344
Pages: 547-553
Description
「研究成果報告書概要(欧文)」より
Related Report
-
[Journal Article] Binding of a novel SMG-1-Upf1-eRF1-eRF3 complex (SURF) to the exon junction complex triggers Upf1 phosphorylation and nonsense-mediated mRNA decay.2006
Author(s)
Kashima, I., Yamashita, A., Izumi, N., Kataoka, N., Morishita, R., Hoshino, S., Ohno, M., Dreyfuss, G., Ohno, S.
-
Journal Title
Genes Dev 20
Pages: 355-367
Description
「研究成果報告書概要(欧文)」より
Related Report
-
[Journal Article] The decapping enzyme Dcp1 participates in translatio termination through its interaction with the release factor eRF3 in budding yeast.2006
Author(s)
Kofuji, S., Sakuno, T., Takahashi, S., Araki, Y., Doi, Y., Hoshino, S., Katada, T
-
Journal Title
Biochem.Biophys.Res.Commun. 344
Pages: 547-547
Related Report
-
[Journal Article] Binding of a novel SMG-1-Upf1-eRF1-eRF3 complex (SURF) to the exon junction complex triggers Upfl phosphorylation and nonsense-mediated mRNA decay.2006
Author(s)
Kashima, I., Yamashita, A., Izumi, N., Kataoka, N., Morishita, R., Hoshino, S., Ohno, M., Dreyfuss, G., Ohno, S
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Journal Title
Genes ＆ Development 20
Pages: 355-355
Related Report
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