• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

1次感覚ニューロンにおけるb系列ガングリオシドの機能的役割

Research Project

Project/Area Number 17046018
Research Category

Grant-in-Aid for Scientific Research on Priority Areas

Allocation TypeSingle-year Grants
Review Section Biological Sciences
Research InstitutionDokkyo Medical University

Principal Investigator

結城 伸泰  獨協医科大学, 医学部, 助教授 (60285913)

Co-Investigator(Kenkyū-buntansha) 小鷹 昌明  獨協医科大学, 医学部, 講師 (50322409)
船越 慶  獨協医科大学, 医学部, 助手 (50406188)
高久 静香  獨協医科大学, 医学部, 助手 (50383013)
Project Period (FY) 2005 – 2006
Project Status Completed (Fiscal Year 2006)
Budget Amount *help
¥5,200,000 (Direct Cost: ¥5,200,000)
Fiscal Year 2006: ¥2,600,000 (Direct Cost: ¥2,600,000)
Fiscal Year 2005: ¥2,600,000 (Direct Cost: ¥2,600,000)
Keywords糖鎖 / 脂質 / 神経科学 / シグナル伝達 / 免疫
Research Abstract

ニューロパチーにより運動失調をきたしうる。感覚性運動失調型のニューロパチーではIgG抗GD1b抗体やb系列ガングリオシドに対するIgM抗体が、小脳性運動失調型のニューロパチーではIgG抗GQ1b抗体が検出されることがある。
本研究では、作業仮説「b系列ガングリオシドが固有感覚を司る1次感覚ニューロンの機能維持に重要な役割を演じている」を検証するため、GD1b、GQ1bを含むb系列ガングリオシドを発現していないマウスの解析を始めた。
GD3合成酵素をノックアウトしたF4マウスとC57BL6を戻し交配し、F8世代のマウスを得た。
固有感覚を司るIa群線維の起始細胞は脊髄神経節に存在し、筋紡錘の錘内筋から筋肉情報(入力)を受け脊髄後根を経由し、脊髄前角に伝達している。前角のγ-運動ニューロンがこれらの情報を受けて、錘内筋に指令を出している。
GD3合成酵素ノックアウトマウスと、その同腹の野生型マウスにおいて、後根神経節におけるパルブアルブミン(深部感覚をつかさどる大型ニューロンのマーカー)、イソレクチンB4(小型ニューロンのマーカー)を用いて免疫組織化学を行い、大型・小型ニューロンの局在をすでに確認した。今後これらで染色される単位面積当たりの神経細胞数を計測する。ノックアウトマウスにおいてパルブアルブミンで染色される細胞数が少なく、イソレクチンB4で染色される細胞数は不変であるという結果が期待される。

Report

(2 results)
  • 2006 Annual Research Report
  • 2005 Annual Research Report
  • Research Products

    (33 results)

All 2007 2006 2005 Other

All Journal Article (33 results)

  • [Journal Article] Recognition characteristics of monoclonal antibodies that are cross-reactive with gangliosides and lipo-oligosaccharide from Campylobacter jejuni strains associated with Guillain-Barrc and Fisher syndromes.2007

    • Author(s)
      Houliston RS
    • Journal Title

      Biochemistry 46

      Pages: 36-44

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Leukocyte and complement activation by GM1-specific antibodies is associated with acute motor axonal neuropathy in rabbits.2007

    • Author(s)
      Van Sorge NM
    • Journal Title

      J Neuroimmunol 182

      Pages: 116-123

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Ganglioside-specific IgG and IgA recruit leukocyte effector functions in Guillain-Barre syndrome.2007

    • Author(s)
      Van Sorge NM
    • Journal Title

      J Neuroimmunol 182

      Pages: 177-184

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Treatment of Miller Fisher syndrome and other related disorders. Treatment for Fisher syndrome, Bickerstaffs brainstem encephalitis and related disorders.2007

    • Author(s)
      Overell J
    • Journal Title

      Cochrane Database Syst Rev 1

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Comprehensive analysis of bacterial risk factors for the development of Guillain-Barre syndrome after Campylobacter jejuni enteritis.2006

    • Author(s)
      Koga M
    • Journal Title

      J Infect Dis 193

      Pages: 547-555

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Rhinolalia after diarrhea : a sole motor symptom occurring in post-infectiousneuropathy associated with anti-ganglioside antibodies.2006

    • Author(s)
      Kamitani T
    • Journal Title

      Eur J Neurol 13

      Pages: 203-204

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Isolated abducens nerve palsy as a regional variant of Guillain-Barre syndrome.2006

    • Author(s)
      Tatsumoto M
    • Journal Title

      J Neurol Sci 243

      Pages: 35-38

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Electrophysiological subtypes and prognosis of childhood Guillain-Barre syndrom in Japan.2006

    • Author(s)
      Nagasawa K
    • Journal Title

      Muscle Nerve 33

      Pages: 766-770

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Identification of a sialate O-acetyltransferase from Campylobacter jejuni : demonstration of direct transfer to the C-9 position of terminal α-2,8-linked sialic acid.2006

    • Author(s)
      Houliston RS
    • Journal Title

      J Biol Chem 281

      Pages: 11480-11486

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Severe cranial nerve involvement in a patient with monoclonal anti-MAG/SGPG IgM antibody and localized hard palate amyloidosis.2006

    • Author(s)
      Yoshida T
    • Journal Title

      J Neurol Sci 244

      Pages: 167-171

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Campylobacter coli enteritis and Guillain-Barre syndrome : no evidence of molecular mimicry and serological relationship.2006

    • Author(s)
      Funakoshi K
    • Journal Title

      J Neurol Sci 246

      Pages: 163-168

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Spectrum of neurological diseases associated with antibodies to minor gangliosides GM1b and GalNAc-GD1a.2006

    • Author(s)
      Tatsumoto M
    • Journal Title

      J Neuroimmunol 177

      Pages: 201-208

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Ganglioside mimicry as a cause of Guillain-Barre syndrome.2006

    • Author(s)
      Komagamine T
    • Journal Title

      CNS & Neurological Disorders-Drug Targets 5

      Pages: 391-400

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Rituximab therapy in chronic inflammatory demyelinating polyradiculoneuropathy with anti-SGPG IgM antibody.2006

    • Author(s)
      Gono T
    • Journal Title

      J Clin Neurosci 13

      Pages: 683-687

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Bacterial infections in Guillain-Barre and Fisher syndromes.2006

    • Author(s)
      Yuki N
    • Journal Title

      Cur Opin Neurol 19

      Pages: 451-457

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Relationship of bacterial strains to clinical syndromes of Campylobacter-associated neuropathies.2006

    • Author(s)
      Kimoto K
    • Journal Title

      Neurology 67

      Pages: 1837-1843

    • Related Report
      2006 Annual Research Report
  • [Journal Article] High affinity of anti-GM1 antibodies associated with disease onset in experimental neuropathy.2006

    • Author(s)
      Comin R
    • Journal Title

      J Neurosci Res 84

      Pages: 1085-1090

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Comprehensive analysis of bacterial risk factors for developing Guillain-Barre syndrome after Campylobacter jejuni enteritis.2006

    • Author(s)
      Koga M
    • Journal Title

      J Infect Dis 193

      Pages: 547-555

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Ambiguous value of Haemophilus influenzae isolation in Guillain-Barre and Fisher syndromes.2005

    • Author(s)
      Koga M
    • Journal Title

      J Neurol Neurosurg Psychiatry 76

      Pages: 1736-1738

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Campylobacter gene polymorphism as a determinant of clinical features of Guillain-Barre syndrome.2005

    • Author(s)
      Koga M
    • Journal Title

      Neurology 65

      Pages: 1376-1381

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Immunoglobulin KM allotypes are associated with the prevalence of autoantibodies to GD1a ganglioside, but not with susceptibility to the disease, in Japanese patients with Guillain-Barre syndrome.2005

    • Author(s)
      Pandey JP
    • Journal Title

      Neurogenetics 6

      Pages: 225-228

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Carbohydrate mimicry : a new paradigm of autoimmune diseases.2005

    • Author(s)
      Yuki N
    • Journal Title

      Curr Opin Immunol 17

      Pages: 577-582

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Electrophoresis-assisted open-tubular liquid chromatography/mass spectrometry for the analysis of lipooligosaccharide expressed by Campylobacter jejuni.2005

    • Author(s)
      Li J
    • Journal Title

      Electrophoresis 26

      Pages: 3360-3368

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Ganglioside mimicry as a cause of Guillain-Barre syndrome.2005

    • Author(s)
      Yuki N
    • Journal Title

      Curr Opin Neurol 18

      Pages: 557-561

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Intractable chronic inflammatory demyelinating polyneuropathy treated successfully with ciclosporin.2005

    • Author(s)
      Odaka M
    • Journal Title

      J Neurol Neurosurg Psychiatry 76

      Pages: 1115-1120

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Side effects of combined therapy of methylprednisolone and intravenous immunoglobulin in Guillain-Barre syndrome.2005

    • Author(s)
      Odaka M
    • Journal Title

      Eur Neurol 53

      Pages: 194-196

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Antecedent infections in Fisher syndrome : a common pathogenesis of molecular mimicry.2005

    • Author(s)
      Koga M
    • Journal Title

      Neurology 64

      Pages: 1605-1611

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Patterns and serial changes in electrodiagnostic abnormalities of axonal Guillain-Barre syndrome.2005

    • Author(s)
      Hiraga A
    • Journal Title

      Neulogyro 64

      Pages: 856-860

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Does Campylobacter jejuni infection elicit "demyelinating" Guillain-Barre syndrome?2005

    • Author(s)
      Kuwabara S
    • Journal Title

      Neulogyro 64

      Pages: 766-767

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Overexpression of GD1a ganglioside sensitizes motor nerve terminals to anti-GD1a antibody-mediated injury in a model of acute motor axonal neuropathy.2005

    • Author(s)
      Goodfellow JA
    • Journal Title

      J Neurosci 25

      Pages: 1620-1628

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Epidemiology of Campylobacter jejuni isolated from patients with Guillain-Barre and Fisher syndromes in Japan.2005

    • Author(s)
      Takahashi M
    • Journal Title

      J Clin Microbiol 43

      Pages: 335-339

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Isolated abducens nerve palsy as a regional variant of Guillain-Barre syndrome.

    • Author(s)
      Tatsumoto M
    • Journal Title

      J Neurol Sci (in press)

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Rhinolalia after diarrhea : a sole motor symptom occurring in postinfectious neuropathy associated with anti-ganglioside antibodies.

    • Author(s)
      Kamitani T
    • Journal Title

      Eur J Neurol (in press)

    • Related Report
      2005 Annual Research Report

URL: 

Published: 2005-04-01   Modified: 2018-03-28  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi