Unified crosstalk of signal transduction, chromatin regulation, and RNA processing by transcription complexes
Project/Area Number |
17054022
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Research Category |
Grant-in-Aid for Scientific Research on Priority Areas
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Allocation Type | Single-year Grants |
Review Section |
Biological Sciences
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Research Institution | University of Toyama |
Principal Investigator |
OHKUMA Yoshiaki University of Toyama, 大学院・医学薬学研究部, 教授 (70192515)
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Co-Investigator(Kenkyū-buntansha) |
廣瀬 豊 富山大学, 大学院医学薬学部, 助手 (00218851)
田中 亜紀 富山大学, 大学院医学薬学部, 助手 (50432109)
高野 克彦 富山大学, 薬学部, 助手 (50293303)
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Co-Investigator(Renkei-kenkyūsha) |
HIROSE Yutaka 富山大学, 大学院・医学薬学研究部, 准教授 (00218851)
TANAKA Aki 富山大学, 大学院・医学薬学研究部, 助教 (50432109)
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Project Period (FY) |
2005 – 2009
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Project Status |
Completed (Fiscal Year 2009)
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Budget Amount *help |
¥77,900,000 (Direct Cost: ¥77,900,000)
Fiscal Year 2009: ¥14,700,000 (Direct Cost: ¥14,700,000)
Fiscal Year 2008: ¥14,700,000 (Direct Cost: ¥14,700,000)
Fiscal Year 2007: ¥17,000,000 (Direct Cost: ¥17,000,000)
Fiscal Year 2006: ¥17,000,000 (Direct Cost: ¥17,000,000)
Fiscal Year 2005: ¥14,500,000 (Direct Cost: ¥14,500,000)
|
Keywords | RNAポリメラーゼII(Pol II) / 基本転写因子TFIIH / CTD / メディエーター複合体 / リン酸化 / コファクター / TFIIE / フォークヘッドドメイン / ルディエーター複合体 / CDK19 / 酵母2ハイブリッド法 / Rpb5 / 基本転写因子 / TFIIEβ / Rpb / MED15 / KIXドメイン / CDK8 / CDK11 / TFIIH / Rpb12 / Znフィンガードメイン |
Research Abstract |
The applicants characterized the functions of two general transcription factors, TFIIE and TFIIH, which assist Pol II for accurate transcription, and the multisubunit Mediator complex which transduces transcriptional activation signals to Pol II in the nucleus. As for Mediator, we demonstrated that two CDK subunits play a role as a molecular switch between positive and negative regulations in transcription, and we further elucidated the molecular mechenism of TFIIH regulation by TFIIE upon binding.
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Report
(6 results)
Research Products
(75 results)
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[Journal Article] Prolyl isomerase Pin1 shares functional similarity with phosphorylated CTD interacting factor PCIF1 in vertebrate cells2009
Author(s)
Yunokuchi, I., Fan, H., Iwamoto, Y., Araki, C., Yuda, M., Umemura, H., Harada, F., Ohkuma, Y., Hirose, Y
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Journal Title
Genes Cells 14
Pages: 1105-1118
Related Report
Peer Reviewed
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[Journal Article] Prolylisomerase Pin1 shares functional similarity with phosphorylated CTD interacting factor PCIF1 in vertebrate cells.2009
Author(s)
Yunokuchi, I., Fan, H., Iwamoto, Y., Araki, C., Yuda, M., Umemura, H., Harada, F., Ohkuma, Y., Hirose, Y.
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Journal Title
Genes Cells 14
Pages: 1105-1118
Related Report
Peer Reviewed
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[Journal Article] Structural insight into the TFIIE/TFIIH: TFIIE and p53 share the binding region on TFIIH2008
Author(s)
Okuda, M., *Tanaka, A., Satoh, M., Takazawa, M., Mizuta, S., Ohkuma, Y., Nishimura, Y
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Journal Title
EMBO J 27
Pages: 1161-1171
Related Report
Peer Reviewed
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[Journal Article] Human Mediator Kinase Subunit CDK11 Plays a Negative Role in Viral Activator VP16-dependent Transcription2008
Author(s)
Tsutsui, T., Umemura, H., Tanaka, A., Mizuki, F., Hirose, Y., Ohkuma, Y
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Journal Title
Genes Cells 13
Pages: 817-826
Related Report
Peer Reviewed
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[Journal Article] Human phosphorylated CTD-interacting protein, PCIF1, negatively modulates gene expression by RNA polymerase II2008
Author(s)
Hirose, Y., *Iwamoto, Y., Sakuraba, K., Yunokuchi, I., Harada, F., Ohkuma, Y
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Journal Title
Biochem. Biophys. Res. Comm 69
Pages: 449-455
Related Report
Peer Reviewed
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[Journal Article] A kinase subunit of the human Mediator complex, CDK8, positively regulates transcriptional activation2007
Author(s)
Furumoto, T., Tanaka, A., Ito, M., Malik, S., Hirose, Y., Hanaoka, F., Ohkuma, Y
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Journal Title
Genes Cells 12
Pages: 119-132
Related Report
Peer Reviewed
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[Journal Article] Studies of Schizosaccharomyces pombe TFIIE indicate conformational and functional changes in RNA polymerase II at transcription initiation2005
Author(s)
Hayashi, K., Watanabe, T., Tanaka, A., Furumoto, T., Sato-Tsuchiya, C., Kimura, M., Yokoi, M., Ishihama, A., Hanaoka, F., Ohkuma, Y
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Journal Title
Genes Cells 10
Pages: 207-224
Related Report
Peer Reviewed
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[Presentation] Mediator MED15 and general transcription factor TFIIE interact to regulate RNA polymerase II transcription2009
Author(s)
Nakatsubo, T., Tanaka, A., Tsutsui, T., Mizuki, F., Ohkuma, Y
Organizer
The 2009 CSH Meeting on Mechanisms of Eukaryotic Transcription
Place of Presentation
Cold Spring Harbor, NY, USA
Year and Date
20090800
Related Report
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[Presentation] Functional characterization of the vertebrate phosphorylated CTD-interacting protein PCIF12009
Author(s)
Hirose, Y., Iwamoto, Y., Yunokuchi, I., Araki, C., Harada, H., Ho, K., Shuman, S., Mizuta, S., Ohkuma, Y
Organizer
The 2009 CSH Meeting on Mechanisms of Eukaryotic Transcription
Place of Presentation
Cold Spring Harbor, NY, USA
Year and Date
20090800
Related Report
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[Presentation] Specific interaction of TFIIEα acidic domain with TFIIH p62 PH domain in regulation of initiation transcription2008
Author(s)
Tanaka, A., Mizuta, S., Takazawa, M., Okuda, M., Nishimura, Y., Ohkuma, Y
Organizer
8th EMBL Transcription Meeting
Place of Presentation
EMBL, Heidelberg, Germany
Year and Date
20080823-20080827
Related Report
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[Presentation] Functional study on novel Mediator subunit CDK11 in transcriptional regulation2008
Author(s)
Tsutsui, T., Umemura, H., Tanaka, A., Mizuki, F., Hirose, Y., Ohkuma, Y
Organizer
8th EMBL Transcription Meeting
Place of Presentation
EMBL, Heidelberg, Germany
Year and Date
20080800
Related Report
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[Presentation] Functional studies on TFIIE and its mediated changes of RNA polymerase II in association with TFIIH and Mediator2007
Author(s)
Nakatsubo, T., Goto, S., Iida, Y., Tanaka, A., Ohkuma, Y
Organizer
10th Cold Spring Harbor Meeting on Cancer Cells: mechanisms of eukaryotic transcription
Place of Presentation
Cold Spring Harbor, NY, USA
Year and Date
20070800
Related Report
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[Presentation] hCDK8, a kinase subunit of human Mediator complex, plays a positive role in transcription2007
Author(s)
Umemura, H., Tsutsui, T., Nakatsubo, T., Tanaka, A., Hirose, Y., Ohkuma, Y
Organizer
10th Cold Spring Harbor Meeting on Cancer Cells
Place of Presentation
Cold Spring Harbor, NY, USA
Year and Date
20070800
Related Report
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[Presentation] Functional characterization of the phosphorylated RNA polymerase II interacting factor PCIF12006
Author(s)
Hirose, Y., Araki, C., Yuda, M., Yunokuchi, I., Fan, H., Harada, F., Ohkuma, Y
Organizer
RNA 2006 Izu "Functional RNAs and Regulatory Machinery"
Place of Presentation
Izunokuni, Japan1
Year and Date
20061200
Related Report
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[Presentation] A kinase subunit of human Mediator complex CDK8 plays a positive role in transcription2006
Author(s)
Furumoto, T., Tanaka, A., Malik, S., Roeder, R.G., Hanaoka, F., Ohkuma, Y
Organizer
7th EMBL Transcription Meeting
Place of Presentation
Heidelberg, Germany
Year and Date
20060800
Related Report
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[Presentation] Functional studies of human Mediator subunits2006
Author(s)
Furumoto, T., Ito, M., Roeder, R.G., Hanaoka, F., Ohkuma, Y
Organizer
20th IUBMB International Congress of Biochemistry and Molecular Biology and 11th FAOBMB Congress
Place of Presentation
Kyoto, Japan
Year and Date
20060600
Related Report
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[Presentation] Functional characterization of the phosphorylated RNA polymerase II interacting factor PCIF12006
Author(s)
Hirose, Y., Yuda, M., Yunokuchi, I., Fan, H., Ho, K., Shuman, S., Harada, F
Organizer
20th IUBMB International Congress of Biochemistry and Molecular Biology and 11th FAOBMB Congress
Place of Presentation
Kyoto, Japan
Year and Date
20060600
Related Report
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[Presentation] TFIIE induces conformational and functional changes in RNA polymerase II during transcription initiation2005
Author(s)
Hayashi, K., Watanabe, T., Tanaka, A., Furumoto, T., Sato-Tsuchiya, C., Yokoi, M., Hanaoka, F., Ohkuma, Y
Organizer
9th Cold Spring Harbor Meeting on Cancer Cells: mechanisms of eukaryotic transcription
Place of Presentation
Cold Spring Harbor, NY, USA
Year and Date
20050900
Related Report
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[Presentation] Functional studies of human Mediator subunit Med18/p28b2005
Author(s)
Furumoto, T., Tanaka, A., Malik, S., Ito, M., Roeder, R.G., Hanaoka, F., Ohkuma, Y
Organizer
9th Cold Spring Harbor Meeting on Cancer Cells: mechanisms of eukaryotic transcription
Place of Presentation
Cold Spring Harbor, NY, USA
Year and Date
20050900
Related Report
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